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The assessment of the protein profiles and oxidant/antioxidat status in conjunctival Brucella melitensis Rev1 vaccinated sheep

Yıl 2021, Cilt: 32 Sayı: 2, 101 - 106, 30.12.2021
https://doi.org/10.35864/evmd.934452

Öz

Brucellosis is a zoonotic and economically significant animal disease worldwide. The most frequently used vaccine to avoid brucellosis in small ruminants is the Rev1 conjunctival Brucella melitensis vaccine. The aim of this study was to investigate the effects of B.melitensis Revl conjunctival vaccine on total protein, albumin, globulin levels, protein profiles and oxidant/antioxidant status in sheep. Ten sheep were used as animal material for this purpose. The bloods taken before vaccination were used as negative control. The sera obtained one month after administration of single dose B.melitensis vaccine were used as experimental materials. The spectrophotometric method estimated total protein, albumin, globulin levels, total antioxidant capacity (TAS), and total oxidant capacity (TOS). Protein profile was determined by sodium-dodecyl-sulphate and native-polyacrylamide gel electrophoresis methods. It was determined that total protein and globulin levels increased slightly in sero positive sheep (P>0.05). There was no difference for protein profiles in both electrophoresis methods. The band densities of albumin decreased but gamma globulin increased slightly after vaccination. TAS levels decreased significantly (P<0.05), but TOS levels increased slightly (P>0.05). In conclusion, Rev-1 conjunctival Brucella vaccine was thought to be safe to use to prevent Brucellosis, and the addition of antioxidant after vaccination can reduce oxidative stress.

Kaynakça

  • Al-Khafaji WS, Al-Farwachi MI. (2012). Antioxidant status in pregnant ewes vaccinated with Rev 1 against brucellosis. Iraqi J Vet Sci. 26(1), 15-19.
  • Alton GG, Elberg SS. (1967). Rev-1 Brucella melitensis vaccine, a review of the 10 years of study. Vet Bull. 37, 793-800.
  • Anderson NL, Anderson NG. (2002). The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Prot. 1(11), 845-867.
  • Aruoma OI. (1996). Characterization of drugs as antioxidant prophylactics. Free Rad Biol Med. 20, 675-705.
  • Blasco JM. (1997). A review of the use of Brucella melitensis Rev-1 vaccine in adult sheep and goats. Prev Vet Med. 31, 275-283.
  • Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, Carnemolla B, Orecchia P, Zardi L, Righetti PG. (2004). Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis. 25(9), 1327-1333.
  • Celi P, Gabai G. (2015). Oxidant/antioxidant balance in animal nutrition and health: The role of protein oxidation. Front Vet Sci. 2, 1-13.
  • Çiftci G, Yiğit Ö, Çiftci A. (2019). The effects of the conjunctival Brucella vaccine on some biochemical parameters in sheep. Trop Anim Health Prod. 51, 355-361.
  • Diaz Aparicio E. (2013). Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Technol. 32(1), 53-60.
  • Dieffenbach CW, Tramont EC. (2005). Innate (general or nonspecific) host defense mechanisms. In: Mandell GL, Bennett JE, Dolin R (eds). Principles and Practice of Infectious Diseases. Sixth edition. Elsevier Churchill Livingstone, Philadelphia; pp.34-42.
  • Eilazab MFA. (2015). Evaluation of serum enzyme activities and protein fractions in Brucella-infected cows. Turk J Vet Anim Sci. 39, 480-484.
  • El-Boshy M, Abbas M, El-Khoderyl H, Osman S. (2009). Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius). Vet Med. 54, 25-32.
  • Erdogan S, Aslantas O, Celik S, Atik E. (2007). The effects of increased cAMP content on inflammation, oxidative stress and PDE4 transcripts during Brucella melitensis infection. Res Vet Sci. 82, 181-186.
  • Erel O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 37(4), 277-285.
  • Erel O. (2005). A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38(12), 1103-1111.
  • Halliwell B, Gutteridge JM. (1999). Free radicals in biology and medicine, 3rd ed., London: Oxford Science Publications.
  • Hamada DM, Mohamed AH, Mabrouk A, Emad M, Ah ME. (2013). Seroprevalence of abortion causing agents in Egyptian sheep and goat breeds and their effects on the animal’s performance. J Agricult Sci. 5, 92-101.
  • Hornback ML, Roop RM. (2006). The Brucella abortus xthA-1 gene product participates in base excision repair and resistance to oxidative killing but is not required for wild-type virulence in the mouse model. J Bacteriol. 188, 1295-1300.
  • Kaplan M. (1966). The problems of choice between control and eradication. Joint WHO/FAO Expert Committee Zoonoses, Geneva, Dec.6-12.
  • Karaagac L, Koruk ST, Koruk I, Aksoy N. (2011). Decreasing oxidative stress in response to treatment in patients with brucellosis: could it be used to monitor treatment? Int J Infect Dis. 15, e346-e349.
  • Kataria N, Kataria AK, Joshi A, Pandey N, Khan S. (2012). Serum Antioxidant Status to Assess Oxidative Stress in Brucella Infected Buffaloes. J Stress Physiol Biochem. 8, 5-9.
  • Kim JA, Sha Z, Mayfield JE. (2006). Regulation of Brucella abortus catalase. Infect Immun. 68, 3861-3866.
  • Laemmli UK. (1970). Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.
  • Marzetti S, Carranza C, Roncallo M, Escobar GI, Lucero NE. (2013). Recent trends in human Brucella canis infection. Compar Immunol Microbiol Infect Dis. 36, 55-61.
  • Merhan O, Bozukluhan K, Kuru M, Büyük F, Özden Ö, Kükürt A. (2017). Investigation of Oxidative Stress Index and Lipid Profile in Cattle with Brucellosis. J Kafkas Univ Vet Fac. 23(6), 933-937.
  • Morag GK. (2002). Veterinary Laboratory Medicine, Clinical Biochemistry and Haematology. 2nd ed. Oxford, UK: Blackwell Science Ltd,.
  • Nath R, Das S, Sarma S, Devi M. (2014). Comparison of blood profiles between healthy and Brucella affected cattle. Vet World. 7(9), 668-670.
  • Nisbet C, Yarim GF, Ciftci A, Cenesiz S, Ciftci G. (2007). Investigation of serum nitric oxide and malondialdehyde levels in cattle infected with Brucella abortus. Vet J Ankara Univ. 54(3), 159-163.
  • Oral H, Ogun M, Kuru M, Kaya S. (2015). Evaluation of certain oxidative stress parameters in heifers that were administered short term PRID. J Kafkas Univ Vet Fac. 21, 569-573.
  • Orem A, Efe H, Deger O, Cimsit G, Uydu HA, Vanizor B. (1997). Relationship between lipid peroxidation and disease activity in patients with Behcet’s disease. J Dermatol Sci. 16, 11-16.
  • Parish CR, Marchalonis JJ. (1970). A simple and rapid acrylamide gel method for estimating the molecular weights of proteins and protein subunits. Anal Biochem. 34(2), 436-450.
  • Perin G, Fávero JF, Severo DRT, Silva AD, Machado G, Araújo HL, Lilenbaum W, Morsch VM, Schetinger MRC, Jordão RS, Stefani LM, Bottari NB, Da Silva AS. (2017). Occurrence of oxidative stress in dairy cows seropositives for Brucella abortus. Microb Pathogen. 29, 196-201.
  • Polycarp TN, Yusoff SM, Benjamin EO, Salisi SM, Khairani S. (2017). Influence of dexamethasone-induced stress on oxidative stres biomarkers in non-pregnant does experimentally infected with Brucella melitensis. Compar Clin Pathol. 26, 423-435.
  • Rada B, Hably C, Meczner A. (2008). Role of Nox2 in elimination of microorganisms. Sem Immunopathol. 30, 237-253.
  • Revappayya M, Basavaraj A, Shambulingappa BE, Surya Prasad V, Abhilash B, Srinivas K. (2017). Evaluation of safety and immunogenicity of Brucella melitensis rev-1 vaccine administered through Conjunctival route in sheep and goats. Int J Biol Sci. 8(2), 103-107.
  • Serefhanoglu K, Taskin A, Turan H, Timurkaynak FE, Arslan H, Erel O. (2009). Evaluation of Oxidative Status in Patients with Brucellosis. Braz J Infect Dis. 13(4), 249-251.
  • Shome R, Gupta VK, Rao KN, Shome BR, Nagalingam M, Rahman H. (2014). Detection of Brucella melitensis Rev–1 vaccinal antibodies in sheep in India. Adv Anim Vet Sci. 2(3S), 19-22.
  • Usta M, Aras Z, Tas A. (2012). Oxidant and antioxidant parameters in patients with Brucella canis. Clin Biochem. 45, 366-367.
  • Vladimirov YA. (2004). Reactive oxygen and nitrogen species diagnostic, preventive and therapy. Biochem. 69(1), 57.
  • Wayner DD, Burton GW, Ingold KU. (1987). The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta. 924, 408-419.
  • World Health Organisation. (1986). Joint FAO/WHO Expert Committee on Brucellosis, Sixth Report, Geneva, pp. 74-75.

Konjunktival Brucella melitensis Rev1 ile aşılanmış koyunlarda protein profillerinin ve oksidan/antioksidan durumunun değerlendirilmesi

Yıl 2021, Cilt: 32 Sayı: 2, 101 - 106, 30.12.2021
https://doi.org/10.35864/evmd.934452

Öz

Bruselloz, dünya çapında zoonotik ve ekonomik açıdan önemli bir hayvan hastalığıdır. Küçükbaş hayvanlarda brusellozdan korunmak için en sık kullanılan aşı Rev1 konjunktival Brucella melitensis aşısıdır. Bu çalışmanın amacı, koyunlarda B. melitensis Revl konjunktival aşısının total protein, albümin, globulin düzeyleri, protein profilleri ve oksidan/antioksidan durumuna etkilerini araştırmaktır. Bu amaçla hayvan materyali olarak on koyun kullanıldı. Aşılama öncesi alınan kanlar negatif kontrol olarak kullanıldı. Tek doz B. melitensis aşısının uygulanmasından bir ay sonra elde edilen serumlar deney materyali olarak kullanıldı. Total protein, albümin, globulin seviyeleri ile antioksidan kapasite (TAS) ve oksidan kapasiteyi (TOS) spektrofotometrik yöntem ile belirlendi. Protein profili, sodyum-dodesil-sülfat ve natif-poliakrilamid jel elektroforez yöntemleri ile belirlendi. Sero-pozitif koyunlarda total protein ve globulin düzeylerinin hafif yükseldiği belirlendi (P> 0.05). Her iki elektroforez yönteminde de protein profilleri açısından fark bulunmadı. Aşılamadan sonra albüminin bant yoğunluklarının azaldığı, ancak gama-globülin yoğunluklarının hafifçe artmış olduğu tespit edildi. TAS düzeyleri önemli ölçüde azalmışken (P <0.05), TOS düzeylerinde biraz artış gözlendi (P> 0.05). Sonuç olarak, Rev-1 konjunktival Brucella aşısının Brucellozdan korunmak için kullanımının güvenli olduğu ve aşılamadan sonra rasyona antioksidan eklenmesinin oksidatif stresi azaltabileceği düşünüldü.

Kaynakça

  • Al-Khafaji WS, Al-Farwachi MI. (2012). Antioxidant status in pregnant ewes vaccinated with Rev 1 against brucellosis. Iraqi J Vet Sci. 26(1), 15-19.
  • Alton GG, Elberg SS. (1967). Rev-1 Brucella melitensis vaccine, a review of the 10 years of study. Vet Bull. 37, 793-800.
  • Anderson NL, Anderson NG. (2002). The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Prot. 1(11), 845-867.
  • Aruoma OI. (1996). Characterization of drugs as antioxidant prophylactics. Free Rad Biol Med. 20, 675-705.
  • Blasco JM. (1997). A review of the use of Brucella melitensis Rev-1 vaccine in adult sheep and goats. Prev Vet Med. 31, 275-283.
  • Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, Carnemolla B, Orecchia P, Zardi L, Righetti PG. (2004). Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis. 25(9), 1327-1333.
  • Celi P, Gabai G. (2015). Oxidant/antioxidant balance in animal nutrition and health: The role of protein oxidation. Front Vet Sci. 2, 1-13.
  • Çiftci G, Yiğit Ö, Çiftci A. (2019). The effects of the conjunctival Brucella vaccine on some biochemical parameters in sheep. Trop Anim Health Prod. 51, 355-361.
  • Diaz Aparicio E. (2013). Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Technol. 32(1), 53-60.
  • Dieffenbach CW, Tramont EC. (2005). Innate (general or nonspecific) host defense mechanisms. In: Mandell GL, Bennett JE, Dolin R (eds). Principles and Practice of Infectious Diseases. Sixth edition. Elsevier Churchill Livingstone, Philadelphia; pp.34-42.
  • Eilazab MFA. (2015). Evaluation of serum enzyme activities and protein fractions in Brucella-infected cows. Turk J Vet Anim Sci. 39, 480-484.
  • El-Boshy M, Abbas M, El-Khoderyl H, Osman S. (2009). Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius). Vet Med. 54, 25-32.
  • Erdogan S, Aslantas O, Celik S, Atik E. (2007). The effects of increased cAMP content on inflammation, oxidative stress and PDE4 transcripts during Brucella melitensis infection. Res Vet Sci. 82, 181-186.
  • Erel O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 37(4), 277-285.
  • Erel O. (2005). A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38(12), 1103-1111.
  • Halliwell B, Gutteridge JM. (1999). Free radicals in biology and medicine, 3rd ed., London: Oxford Science Publications.
  • Hamada DM, Mohamed AH, Mabrouk A, Emad M, Ah ME. (2013). Seroprevalence of abortion causing agents in Egyptian sheep and goat breeds and their effects on the animal’s performance. J Agricult Sci. 5, 92-101.
  • Hornback ML, Roop RM. (2006). The Brucella abortus xthA-1 gene product participates in base excision repair and resistance to oxidative killing but is not required for wild-type virulence in the mouse model. J Bacteriol. 188, 1295-1300.
  • Kaplan M. (1966). The problems of choice between control and eradication. Joint WHO/FAO Expert Committee Zoonoses, Geneva, Dec.6-12.
  • Karaagac L, Koruk ST, Koruk I, Aksoy N. (2011). Decreasing oxidative stress in response to treatment in patients with brucellosis: could it be used to monitor treatment? Int J Infect Dis. 15, e346-e349.
  • Kataria N, Kataria AK, Joshi A, Pandey N, Khan S. (2012). Serum Antioxidant Status to Assess Oxidative Stress in Brucella Infected Buffaloes. J Stress Physiol Biochem. 8, 5-9.
  • Kim JA, Sha Z, Mayfield JE. (2006). Regulation of Brucella abortus catalase. Infect Immun. 68, 3861-3866.
  • Laemmli UK. (1970). Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.
  • Marzetti S, Carranza C, Roncallo M, Escobar GI, Lucero NE. (2013). Recent trends in human Brucella canis infection. Compar Immunol Microbiol Infect Dis. 36, 55-61.
  • Merhan O, Bozukluhan K, Kuru M, Büyük F, Özden Ö, Kükürt A. (2017). Investigation of Oxidative Stress Index and Lipid Profile in Cattle with Brucellosis. J Kafkas Univ Vet Fac. 23(6), 933-937.
  • Morag GK. (2002). Veterinary Laboratory Medicine, Clinical Biochemistry and Haematology. 2nd ed. Oxford, UK: Blackwell Science Ltd,.
  • Nath R, Das S, Sarma S, Devi M. (2014). Comparison of blood profiles between healthy and Brucella affected cattle. Vet World. 7(9), 668-670.
  • Nisbet C, Yarim GF, Ciftci A, Cenesiz S, Ciftci G. (2007). Investigation of serum nitric oxide and malondialdehyde levels in cattle infected with Brucella abortus. Vet J Ankara Univ. 54(3), 159-163.
  • Oral H, Ogun M, Kuru M, Kaya S. (2015). Evaluation of certain oxidative stress parameters in heifers that were administered short term PRID. J Kafkas Univ Vet Fac. 21, 569-573.
  • Orem A, Efe H, Deger O, Cimsit G, Uydu HA, Vanizor B. (1997). Relationship between lipid peroxidation and disease activity in patients with Behcet’s disease. J Dermatol Sci. 16, 11-16.
  • Parish CR, Marchalonis JJ. (1970). A simple and rapid acrylamide gel method for estimating the molecular weights of proteins and protein subunits. Anal Biochem. 34(2), 436-450.
  • Perin G, Fávero JF, Severo DRT, Silva AD, Machado G, Araújo HL, Lilenbaum W, Morsch VM, Schetinger MRC, Jordão RS, Stefani LM, Bottari NB, Da Silva AS. (2017). Occurrence of oxidative stress in dairy cows seropositives for Brucella abortus. Microb Pathogen. 29, 196-201.
  • Polycarp TN, Yusoff SM, Benjamin EO, Salisi SM, Khairani S. (2017). Influence of dexamethasone-induced stress on oxidative stres biomarkers in non-pregnant does experimentally infected with Brucella melitensis. Compar Clin Pathol. 26, 423-435.
  • Rada B, Hably C, Meczner A. (2008). Role of Nox2 in elimination of microorganisms. Sem Immunopathol. 30, 237-253.
  • Revappayya M, Basavaraj A, Shambulingappa BE, Surya Prasad V, Abhilash B, Srinivas K. (2017). Evaluation of safety and immunogenicity of Brucella melitensis rev-1 vaccine administered through Conjunctival route in sheep and goats. Int J Biol Sci. 8(2), 103-107.
  • Serefhanoglu K, Taskin A, Turan H, Timurkaynak FE, Arslan H, Erel O. (2009). Evaluation of Oxidative Status in Patients with Brucellosis. Braz J Infect Dis. 13(4), 249-251.
  • Shome R, Gupta VK, Rao KN, Shome BR, Nagalingam M, Rahman H. (2014). Detection of Brucella melitensis Rev–1 vaccinal antibodies in sheep in India. Adv Anim Vet Sci. 2(3S), 19-22.
  • Usta M, Aras Z, Tas A. (2012). Oxidant and antioxidant parameters in patients with Brucella canis. Clin Biochem. 45, 366-367.
  • Vladimirov YA. (2004). Reactive oxygen and nitrogen species diagnostic, preventive and therapy. Biochem. 69(1), 57.
  • Wayner DD, Burton GW, Ingold KU. (1987). The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta. 924, 408-419.
  • World Health Organisation. (1986). Joint FAO/WHO Expert Committee on Brucellosis, Sixth Report, Geneva, pp. 74-75.
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Bilimleri
Bölüm Araştırma Makaleleri
Yazarlar

Gülay Çiftci 0000-0001-5384-2381

Alper Çiftci 0000-0001-8370-8677

Erken Görünüm Tarihi 30 Aralık 2021
Yayımlanma Tarihi 30 Aralık 2021
Gönderilme Tarihi 7 Mayıs 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 32 Sayı: 2

Kaynak Göster

APA Çiftci, G., & Çiftci, A. (2021). The assessment of the protein profiles and oxidant/antioxidat status in conjunctival Brucella melitensis Rev1 vaccinated sheep. Etlik Veteriner Mikrobiyoloji Dergisi, 32(2), 101-106. https://doi.org/10.35864/evmd.934452


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