Öz
Objective: This study aimed to reveal variations in HSP90AA1 gene region in Pırlak sheep breed.
Material and Methods: A total of 100 animals randomly chosen from representative herds raised in Antalya province were genotyped by Allele-Specific Polymerase Chain Reaction (AS-PCR) technique and genotypes were validated via agarose gel electrophoresis.
Results: HSP90AA1 region was found to be polymorphic in which two alleles (C and G) and three possible genotypes (CC, CG and GG) were detected. The frequencies of C and G alleles were 0.39 and 0.61, respectively, whereas genotype frequency ranged from 0.183 (CC) to 0.413 (GG). The frequency of CG genotype was calculated as 0.404. Conservation of the genetic variations at HSP90AA1 region will be required for planning selection programs against heat stress in the future.
Conclusion: HSP90AA1 and similar genes may offer new opportunities to reduce heat stress caused by global climate change in the near future. Additionally, more genetic studies for the other genomic regions related to environmental stressors should be conducted in Pırlak breed in order to shape conservation studies.
Anahtar Kelimeler
Pırlak sheep AS-PCR characterization heat stress heat tolerance HSP90AA1 polymorphism
Destekleyen Kurum
Scientific and Technological Research Council of Turkey
Proje Numarası
1919B012101337
Teşekkür
This study was financially supported by the Scientific and Technological Research Council of Turkey (Project No: 1919B012101337).
Kaynakça
- Badri TM, Chen KL, Alsiddig MA, Li L, Cai Y, Wang GL. 2018. Genetic polymorphism in Hsp90AA1 gene is associated with the thermotolerance in Chinese Holstein cows. Cell Stress and Chaperones 23: 639-651.
- Chen ZY, Gan JK, Xiao X, Jiang LY, Zhang XQ, Luo QB. 2013. The association of SNPs in Hsp90β gene 5′ flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds. Molecular Biology Reports, 40: 5295-5306.
- Çelikeloğlu K, Erdoğan M, Hacan Ö, Koçak S, Bozkurt Z, Tekerli M. 2018. Pırlak koyunlarında BMPR1B, BMP15 ve GDF9 genlerinde olası polimorfizmlerin araştırılması. Kocatepe Veterinary Journal 11(4): 356-362.
- Demir E, Bilginer U, Balcioglu MS, Karsli T. 2021. Direct and indirect contributions of molecular genetics to farm animal welfare: a review. Animal Health Research Reviews 22(2): 177-186.
- Demir E, Ceccobelli S, Bilginer U, Pasquini M, Attard G, Karsli T. 2022. Conservation and selection of genes related to environmental adaptation in native small ruminant breeds: a review. Ruminants 2(2): 255-270.
- Hoffmann G, Herbut P, Pinto S, Heinicke J, Kuhla B, Amon T. 2020. Animal-related, non-invasive indicators for determining heat stress in dairy cows. Biosystems Engineering 199: 83-96.
- Karsli BA, Demir E, Fidan HG, Karsli T. 2020. Assessment of genetic diversity and differentiation among four indigenous Turkish sheep breeds using microsatellites. Archives Animal Breeding 63(1): 165-172.
- Kumar D, Yadav B, Choudhury S, Kumari P, Madan AK, Singh SP, Routh PK, Ramchandran N, Yadav S. 2018. Evaluation of adaptability to different seasons in goat breeds of semi-arid region in India through differential expression pattern of heat shock protein genes. Biological Rhythm Research 49(3): 466-478.
- Marcos-Carcavilla A, Calvo JH, González C, Moazami-Goudarzi K, Laurent P, Bertaud M, Hayes H, Beattie AE, Serrano C, Lyahyai J, Martín-Burriel I, Serrano M. 2008. Structural and functional analysis of the HSP90AA1 gene: distribution of polymorphisms among sheep with different responses to scrapie. Cell Stress and Chaperones 13: 19-29.
- Miller S, Dykes D, Polesky H. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16(3): 1215-1215.
- Niyas PA, Chaidanya K, Shaji S, Sejian V, Bhatta R. 2015. Adaptation of livestock to environmental challenges. Journal of Veterinary Science and Medical Diagnosis 4(3): 1-7.
- Oner Y, Calvo JH, Serrano M, Elmaci, C. 2012. Polymorphisms at the 5′ flanking region of the HSP90AA1 gene in native Turkish sheep breeds. Livestock Science 150(1-3): 381-385.
- Park H, Ahn IY, Lee HE 2007. Expression of heat shock protein 70 in the thermally stressed Antarctic clam Laternula elliptica. Cell Stress and Chaperones 12(3): 275-282.
- Peakall R, Smouse PE 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformics 28: 2537-2539.
- Salces-Ortiz J, Ramon M, Gonzalez C, Perez-Guzman MD, Garde JJ, Garcia-Alvarez O, Maroto-Morales A, Calvo JH, Serrano MM. 2015. Differences in the ovine HSP90AA1 gene expression rates caused by two linked polymorphisms at its promoter affect rams sperm DNA fragmentation under environmental heat stress conditions. PLoS One 10(2): e0116360.
- Sancar MC, Güngör Ş. 2022. Analysis of Antalya province bioclimatic comfort features according to heat index. International Journal of Eurasia Social Sciences 13(48): 710-723.
- Singh KM, Singh S, Ganguly I, Nachiappan RK, Ganguly A, Venkataramanan R, Chopra A, Narula HK. 2017. Association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in Indian sheep (Ovis aries). Cell Stress and Chaperones 22: 675-684.
Öz
Amaç: Bu çalışmanın amacı Pırlak koyun ırkında HSP90AA1 geni varyasyonlarının incelenmesidir.
Materyal ve Metot: Antalya ilinde ırkı temsil eden sürülerden rastgele seçilen toplam 100 akraba olmayan hayvan Allel-Spesifik Polimeraz Zincir Reaksiyonu (AS-PZR) tekniğiyle genotiplendirilmiş ve genotipler agaroz jel elektorforeziyle tespit edilmiştir.
Bulgular: İki allel (C ve G) ve olası üç genotipin (CC, CG ve GG) tespit edildiği HSP90AA1 bölgesinin polimorfik olduğu belirlenmiştir. C ve G allel frekansı sırasıyla 0.39 ve 0.61 idi, genotip frekansı ise 0.183 (CC) ve 0.413 (GG) aralığında değişmiştir. CG genotip frekansı 0.404 olarak hesaplanmıştır. HSP90AA1 bölgesindeki varyasyonların korunması gelecekte ısı stresine karşı yapılacak olan seleksiyon programlarının planlanması için gerekli olacaktır.
Sonuç: HSP90AA1 ve benzeri genler yakın gelecekte iklim değişikliğinden kaynaklana ısı stresinin azaltılması için yeni fırsatlar sunabilmektedir. Ayrıca, Pırlak koyunlarında koruma çalışmalarının şekillendirilmesi için çevresel stresörlerle ilişkili diğer genomik bölgeler temelinde daha fazla çalışmanın yapılması gerekmektedir.
Anahtar Kelimeler
Pırlak koyunu AS-PZR karakterizasyon ısı stresi ısı toleransı HSP90AA1 polimorfizm
Proje Numarası
1919B012101337
Kaynakça
- Badri TM, Chen KL, Alsiddig MA, Li L, Cai Y, Wang GL. 2018. Genetic polymorphism in Hsp90AA1 gene is associated with the thermotolerance in Chinese Holstein cows. Cell Stress and Chaperones 23: 639-651.
- Chen ZY, Gan JK, Xiao X, Jiang LY, Zhang XQ, Luo QB. 2013. The association of SNPs in Hsp90β gene 5′ flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds. Molecular Biology Reports, 40: 5295-5306.
- Çelikeloğlu K, Erdoğan M, Hacan Ö, Koçak S, Bozkurt Z, Tekerli M. 2018. Pırlak koyunlarında BMPR1B, BMP15 ve GDF9 genlerinde olası polimorfizmlerin araştırılması. Kocatepe Veterinary Journal 11(4): 356-362.
- Demir E, Bilginer U, Balcioglu MS, Karsli T. 2021. Direct and indirect contributions of molecular genetics to farm animal welfare: a review. Animal Health Research Reviews 22(2): 177-186.
- Demir E, Ceccobelli S, Bilginer U, Pasquini M, Attard G, Karsli T. 2022. Conservation and selection of genes related to environmental adaptation in native small ruminant breeds: a review. Ruminants 2(2): 255-270.
- Hoffmann G, Herbut P, Pinto S, Heinicke J, Kuhla B, Amon T. 2020. Animal-related, non-invasive indicators for determining heat stress in dairy cows. Biosystems Engineering 199: 83-96.
- Karsli BA, Demir E, Fidan HG, Karsli T. 2020. Assessment of genetic diversity and differentiation among four indigenous Turkish sheep breeds using microsatellites. Archives Animal Breeding 63(1): 165-172.
- Kumar D, Yadav B, Choudhury S, Kumari P, Madan AK, Singh SP, Routh PK, Ramchandran N, Yadav S. 2018. Evaluation of adaptability to different seasons in goat breeds of semi-arid region in India through differential expression pattern of heat shock protein genes. Biological Rhythm Research 49(3): 466-478.
- Marcos-Carcavilla A, Calvo JH, González C, Moazami-Goudarzi K, Laurent P, Bertaud M, Hayes H, Beattie AE, Serrano C, Lyahyai J, Martín-Burriel I, Serrano M. 2008. Structural and functional analysis of the HSP90AA1 gene: distribution of polymorphisms among sheep with different responses to scrapie. Cell Stress and Chaperones 13: 19-29.
- Miller S, Dykes D, Polesky H. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16(3): 1215-1215.
- Niyas PA, Chaidanya K, Shaji S, Sejian V, Bhatta R. 2015. Adaptation of livestock to environmental challenges. Journal of Veterinary Science and Medical Diagnosis 4(3): 1-7.
- Oner Y, Calvo JH, Serrano M, Elmaci, C. 2012. Polymorphisms at the 5′ flanking region of the HSP90AA1 gene in native Turkish sheep breeds. Livestock Science 150(1-3): 381-385.
- Park H, Ahn IY, Lee HE 2007. Expression of heat shock protein 70 in the thermally stressed Antarctic clam Laternula elliptica. Cell Stress and Chaperones 12(3): 275-282.
- Peakall R, Smouse PE 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformics 28: 2537-2539.
- Salces-Ortiz J, Ramon M, Gonzalez C, Perez-Guzman MD, Garde JJ, Garcia-Alvarez O, Maroto-Morales A, Calvo JH, Serrano MM. 2015. Differences in the ovine HSP90AA1 gene expression rates caused by two linked polymorphisms at its promoter affect rams sperm DNA fragmentation under environmental heat stress conditions. PLoS One 10(2): e0116360.
- Sancar MC, Güngör Ş. 2022. Analysis of Antalya province bioclimatic comfort features according to heat index. International Journal of Eurasia Social Sciences 13(48): 710-723.
- Singh KM, Singh S, Ganguly I, Nachiappan RK, Ganguly A, Venkataramanan R, Chopra A, Narula HK. 2017. Association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in Indian sheep (Ovis aries). Cell Stress and Chaperones 22: 675-684.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Küçükbaş Hayvan Yetiştirme ve Islahı, Zootekni, Genetik ve Biyoistatistik |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Proje Numarası | 1919B012101337 |
| Erken Görünüm Tarihi | 19 Eylül 2023 |
| Yayımlanma Tarihi | 30 Ekim 2023 |
| Gönderilme Tarihi | 21 Mart 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 64 Sayı: 1 |
Cited By
Determination of polymorphisms in the HSP90AA1 gene region in some Turkish sheep populations by AS-PCR
Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi
https://doi.org/10.37908/mkutbd.1351101
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