Araştırma Makalesi
Protective effects of vitamin C on hormonal level and testicular histopathology of rabbit bucks with metronidazole-induced toxicity
Öz
Objective: This study evaluates the protective effects of Vitamin C on hormonal level and testicular histopathology in rabbit bucks with metronidazole-induced toxicity. Methods: Twenty adult rabbit bucks which were weighed and divided into four groups with five in each group were used for the study. Group I is the control, group II was given metronidazole 400 mg/kg/day for 30 days, group III was co-administered 400 mg/kg/day of metronidazole and 200 mg/kg/day of Vitamin C for 30 days and group IV was given 200 mg/kg/day Vitamin C for 30 days. At the end of the experiment, the rabbit bucks were weighed, and blood samples were collected from the marginal ear vein into a plain bottle and serum extracted through centrifugation for hormonal assay. FSH, LH and Testosterone assay were carried out using Enzyme Linked Immunosorbent Assay (ELISA) kits according to manufacturers’ instruction. One testis from each rabbit was removed for testicular histology. Results: The study found out that there was no significant difference in the body weights of the rabbit bucks before and after the experiment, metronidazole significantly (p < 0.05) affects hormonal concentration in the bucks and there was significant improvement following vitamin C administration. The study also found out that metronidazole caused testicular degeneration which was reversed by Vitamin C administration. Conclusion: Vitamin C has protective effect against metronidazole-induced toxicity and its use in therapeutic application in prolong use of metronidazole is recommended.
Anahtar Kelimeler
Etik Beyan
Ethical approval was sought from University of Ilorin Research Ethics Committee with approval No.: UREC/FVM/PG20/68VO002 before commencement of the research and ethical guidelines for the use of animal was strictly followed.
Destekleyen Kurum
University of Ilorin
Kaynakça
- Akorede, G. J., Ambali S. F., Hudu M. G., Olatunji, A. O., Shittu, M., Aremu, A., Basiru, A., Biobaku, K. T., Ahmed A. O., & Ameen S. A. (2020). Protective effect of vitamin C on chronic carbamazepine-induced reproductive toxicity in male wistar rats. Toxicology Reports 7, 269–276.
- Ashamu, E. A., Salawu, E. O., & Oyewo, O. O. (2010). Efficacy of vitamin C and ethanolic extract of Sesamum indicum in promoting fertility in male Wistar rats. Journal of Human Reproductive Sciences, 3(1), 11-14.
- Benabbou, A., Meghit, B. K., & Ali, S. A. (2017). Evaluation of the efficiency of combined and separated antioxidant supplementation of Vitamin C and E on semen parameters in strepto-zotocin-induced diabetic male Wistar rats. South Asian Journal of Experimental Biology, 7(4): 166-172.
- Bergan, T. (1985). Antibacterial activity and pharmacokinetics of nitroimidazole. A review. Scandinavian Journal of Infectious Diseases, 46, 64-71.
- Castellini, C. (2008). Semen production and management of rabbit bucks, Reproduction, 9th World Rabbit Congress , 265-278.
- Davood, S., Mohsen, A., & Ali, A. M. (2007). Effect of metronidazole on spermatogenesis, plasma gonadotrophins and testosterone in rats. Iranian Journal of Reproductive Medicine, 5(2), 69-72.
- Dixon, R. L., & Lee, I. P. (1977). Possible role of the blood-testis barrier in dominant lethal testing. Environmental Health Perspectives, 6, 59-63.
- Egba, S. I., Sunday, G. I., & Anaduaka, E. G. (2014). The effect of oral administration of aqueous extract of Newbouldia laevis leaves on fertility hormones of male albino rats. Journal of Pharmacy and Biological Sciences, 9(3), 61-64.
- Ekaluo, U. B., Ikpeme, E. V., & Ibiang, Y. B. (2013). Attenuating role of vitamin C on sperm toxicity induced by monosodium glutamate in albino rats. Journal of Biological Sciences, 13, 298-301.
- Fernandes, G. S., Fernandez, C. D., Campos, K. E., Damasceno, D. C., Anselmo-Franci, J. A., & Kempinas, W. D. (2011). Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats. Reproductive Biology and Endocrinology, 9, 100.
- Foote, R. H. (2002). Effects of metronidazole, ipronidazole, and dibromochloropropane on rabbit and human sperm motility and fertility. Reproductive toxicology (Elmsford, N.Y.), 16(6), 749–755.
- Grover, J. K., Vats, V., Srinavas, M., Das, S. N., Jha, P., & Gupta, D. K. (2001). Effect of metronidazole on spermatogenesis and FSH, LH and testosterone levels of pre-Pubertal rats. Indian Journal of Experimental Biology 39, 1160-1166.
- Kumari, M., & Singh, P. (2015). Tribulus terrestris ameliorates metronidazole-induced spermatogenic inhibition and testicular oxidative stress in the laboratory mouse. Indian Journal of Pharmacology, 47, 304-10.
- Luna, G.H. (1960). Manual of Histologic Staining Method of Armed Forces Institute of Pathology, 35th edition, McGraw-Hill Book Company, New York, p. p258.
- O’Donnel, L. McLachlan, R. I., Wreford, N. G., & Robertson, D. M. (1994). Testosterone promotes the conversion of round spermatids between stages vii and viii of the rat spermatogenic cycle. Endocrinology, 135, 2608-2614.
- Osuntokun, O. S., Olayiwola, G., Oladele, A., Ola, I., & Ayoka, A. O. (2017). Chronic administration of gabapentin and a gabapentin-carbamazepine combination reversibly suppress testicular function in male Wistar rats (Rattus norvegicus). Pathophysiology, 24, 63-69.
- Oyedeji, K. O., Oshatimi, A., Abidoye, D., & Adeleke, K. O., (2015). Effect of Metronidazole on Reproductive Parameters in Male Wistar Rats. International Journal of Pharmaceutical Sciences Review and Research, 35(1), 186-190.
- Rhayf, A. G., Naji, H. A., & Hassan, N. F. (2014). Toxic effect of metronidazole on reproductive system in male rabbits 27 (336–323) Shreen Al-Tha - Special Issue for the First Scientific Conference of the College of Veterinary Medicine – Al-Qasim Green University.
- Roy, D.L., Giri, S., Singh, S., & Giri, A. (2013). Effects of radiation and vitamin C treatment on metronidazole genotoxicity in mice. Mutation Research, 753(2), 65–71.
- Saad, T. R., Ismael, I. H., & Mustafa, T. K. (2018). Study the effect of metronidazole drug (MTZ) and rhuscoriaria (Sumac) on testicular tissues and sperms of male white mice. Tikrit Journal of Pure Science, 23(1), 38-42.
- Sadeghzadeh, F., Mehranjani, M. S., & Mahmoodi, M. (2019). Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice. Human and Experimental Toxicology, 38(4), 409-418.
- Samah, S. O. (2012). Histopathological and biochemical alterations of metronidazole-induced toxicity in male rats. Global Veterinaria 9(3), 303-310.
- Sharpe, R.M. (1987). Testosterone and spermatogenesis, Journal of Endocrinology, 113, 1-2.
- Shyr C., Collins L., Mu X., Platt K., & Chang C. (2002). Spermatogenesis and testis development are normal in mice lacking testicular orphan nuclear receptor 2. Molecular and Cellular Biology, 22, 4661-4666.
- Sonmez, M., Turk, G., & Yuce, A. (2005). The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats, Theriogenology 63, 2063–2072.
- Vijay, P., Yeshwanth, R., & Bairy, K. L. (2009). Effect of phenytoin sodium on the biochemical parameters of reproductive function in male albino Wistar rats. Journal of Physiological and Biomedical Sciences, 14-18.
Yıl 2023,
Cilt: 7 Sayı: 3, 112 - 117, 31.12.2023
Öz
Kaynakça
- Akorede, G. J., Ambali S. F., Hudu M. G., Olatunji, A. O., Shittu, M., Aremu, A., Basiru, A., Biobaku, K. T., Ahmed A. O., & Ameen S. A. (2020). Protective effect of vitamin C on chronic carbamazepine-induced reproductive toxicity in male wistar rats. Toxicology Reports 7, 269–276.
- Ashamu, E. A., Salawu, E. O., & Oyewo, O. O. (2010). Efficacy of vitamin C and ethanolic extract of Sesamum indicum in promoting fertility in male Wistar rats. Journal of Human Reproductive Sciences, 3(1), 11-14.
- Benabbou, A., Meghit, B. K., & Ali, S. A. (2017). Evaluation of the efficiency of combined and separated antioxidant supplementation of Vitamin C and E on semen parameters in strepto-zotocin-induced diabetic male Wistar rats. South Asian Journal of Experimental Biology, 7(4): 166-172.
- Bergan, T. (1985). Antibacterial activity and pharmacokinetics of nitroimidazole. A review. Scandinavian Journal of Infectious Diseases, 46, 64-71.
- Castellini, C. (2008). Semen production and management of rabbit bucks, Reproduction, 9th World Rabbit Congress , 265-278.
- Davood, S., Mohsen, A., & Ali, A. M. (2007). Effect of metronidazole on spermatogenesis, plasma gonadotrophins and testosterone in rats. Iranian Journal of Reproductive Medicine, 5(2), 69-72.
- Dixon, R. L., & Lee, I. P. (1977). Possible role of the blood-testis barrier in dominant lethal testing. Environmental Health Perspectives, 6, 59-63.
- Egba, S. I., Sunday, G. I., & Anaduaka, E. G. (2014). The effect of oral administration of aqueous extract of Newbouldia laevis leaves on fertility hormones of male albino rats. Journal of Pharmacy and Biological Sciences, 9(3), 61-64.
- Ekaluo, U. B., Ikpeme, E. V., & Ibiang, Y. B. (2013). Attenuating role of vitamin C on sperm toxicity induced by monosodium glutamate in albino rats. Journal of Biological Sciences, 13, 298-301.
- Fernandes, G. S., Fernandez, C. D., Campos, K. E., Damasceno, D. C., Anselmo-Franci, J. A., & Kempinas, W. D. (2011). Vitamin C partially attenuates male reproductive deficits in hyperglycemic rats. Reproductive Biology and Endocrinology, 9, 100.
- Foote, R. H. (2002). Effects of metronidazole, ipronidazole, and dibromochloropropane on rabbit and human sperm motility and fertility. Reproductive toxicology (Elmsford, N.Y.), 16(6), 749–755.
- Grover, J. K., Vats, V., Srinavas, M., Das, S. N., Jha, P., & Gupta, D. K. (2001). Effect of metronidazole on spermatogenesis and FSH, LH and testosterone levels of pre-Pubertal rats. Indian Journal of Experimental Biology 39, 1160-1166.
- Kumari, M., & Singh, P. (2015). Tribulus terrestris ameliorates metronidazole-induced spermatogenic inhibition and testicular oxidative stress in the laboratory mouse. Indian Journal of Pharmacology, 47, 304-10.
- Luna, G.H. (1960). Manual of Histologic Staining Method of Armed Forces Institute of Pathology, 35th edition, McGraw-Hill Book Company, New York, p. p258.
- O’Donnel, L. McLachlan, R. I., Wreford, N. G., & Robertson, D. M. (1994). Testosterone promotes the conversion of round spermatids between stages vii and viii of the rat spermatogenic cycle. Endocrinology, 135, 2608-2614.
- Osuntokun, O. S., Olayiwola, G., Oladele, A., Ola, I., & Ayoka, A. O. (2017). Chronic administration of gabapentin and a gabapentin-carbamazepine combination reversibly suppress testicular function in male Wistar rats (Rattus norvegicus). Pathophysiology, 24, 63-69.
- Oyedeji, K. O., Oshatimi, A., Abidoye, D., & Adeleke, K. O., (2015). Effect of Metronidazole on Reproductive Parameters in Male Wistar Rats. International Journal of Pharmaceutical Sciences Review and Research, 35(1), 186-190.
- Rhayf, A. G., Naji, H. A., & Hassan, N. F. (2014). Toxic effect of metronidazole on reproductive system in male rabbits 27 (336–323) Shreen Al-Tha - Special Issue for the First Scientific Conference of the College of Veterinary Medicine – Al-Qasim Green University.
- Roy, D.L., Giri, S., Singh, S., & Giri, A. (2013). Effects of radiation and vitamin C treatment on metronidazole genotoxicity in mice. Mutation Research, 753(2), 65–71.
- Saad, T. R., Ismael, I. H., & Mustafa, T. K. (2018). Study the effect of metronidazole drug (MTZ) and rhuscoriaria (Sumac) on testicular tissues and sperms of male white mice. Tikrit Journal of Pure Science, 23(1), 38-42.
- Sadeghzadeh, F., Mehranjani, M. S., & Mahmoodi, M. (2019). Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice. Human and Experimental Toxicology, 38(4), 409-418.
- Samah, S. O. (2012). Histopathological and biochemical alterations of metronidazole-induced toxicity in male rats. Global Veterinaria 9(3), 303-310.
- Sharpe, R.M. (1987). Testosterone and spermatogenesis, Journal of Endocrinology, 113, 1-2.
- Shyr C., Collins L., Mu X., Platt K., & Chang C. (2002). Spermatogenesis and testis development are normal in mice lacking testicular orphan nuclear receptor 2. Molecular and Cellular Biology, 22, 4661-4666.
- Sonmez, M., Turk, G., & Yuce, A. (2005). The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats, Theriogenology 63, 2063–2072.
- Vijay, P., Yeshwanth, R., & Bairy, K. L. (2009). Effect of phenytoin sodium on the biochemical parameters of reproductive function in male albino Wistar rats. Journal of Physiological and Biomedical Sciences, 14-18.
Toplam 26 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Bilimleri (Diğer) |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 31 Aralık 2023 |
| Gönderilme Tarihi | 24 Ekim 2023 |
| Kabul Tarihi | 25 Aralık 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 7 Sayı: 3 |
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