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Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi

Yıl 2018, Cilt: 7 Sayı: 1, 119 - 125, 04.07.2018
https://doi.org/10.31196/huvfd.470988

Öz

Naringenin,
insan sağlığı üzerinde biyoaktif bir etkiye sahip olup, greyfurtta baskın
bulunan doğal bir flavonondur. Bu çalışmada ratlarda sisplatin ile oluşturulan
nefrotoksisite üzerine naringenin’in böbrek dokusundaki bazı biyokimyasal
parametreler üzerine etkileri araştırıldı. Bu çalışmada, 35 adet 2 aylık wistar
albino ratlar kullanıldı. Ratlar rastgele her grupta 7 rat olacak şekilde 5
gruba ayrıldı. 1.grup (Kontrol) %1’lik DMSO i.p, 2.grup  (Cis), tek doz sisplatin., 7 mg/kg / i.p,
3.grup (NG20) naringenin, 20 mg/kg/10 gün /i.p, 4.grup, (Cis+NG20
) tekdoz sisplatin 7 mg/kg/ i.p + 20 mg/kg/10 gün./i.p naringenin, 5.grup
(Cis+NG40) tek doz sisplatin 7 mg/kg/ i.p + 40 mg/kg/10 gün./i.p
naringenin  on gün boyunca uygulandı.
Çalışma sonunda ratlardan alınan böbrek dokusundan biyokimyasal analizler
yapıldı. Sisplatin grubunda böbrek TOS, OSI, MDA, AOPP, 8-OHdG ve NRF-2
düzeyleri kontrol grubuna göre artarken (P<0.05), böbrek TAS ve GSH
(P<0.05) düzeyleri anlamlı olarak azaldı. Sisplatinin ratlarda oluşturduğu
nefrotoksisiteyi, naringenin’in anlamlı olarak azalttığından dolayı, sisplatin'e
bağlı nefrotoksisitenin naringenin ile kontrol edilebileceği sonucuna
varılmıştır.

Kaynakça

  • Ali BH, Al Moundhri M, 2006: Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food and Chemical Toxicology, 44, 8, 1173-1183.
  • Arul D, Subramanian P, 2013: Inhibitory effect of naringenin (citrus flavonone) on N-nitrosodiethylamine induced hepatocarcinogenesis in rats. Biochemical and Biophysical Research Communications, 434, 2, 203-209.
  • Cadet J, Douki T, Gasparutto D, Ravanat J, 2003: Oxidative damage to DNA: formation, measurement and biochemical features. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 531, 1, 5-23.
  • Coşkun NM, Hatipoğlu T, Özoğul C, Korkmaz C, Akyol SN, Mıcılı SC, Arık GS, Erdoğan D, 2013: The protective effects of acetyl L-carnitine on testis gonadotoxicity induced by cisplatin in rats. Balkan Medical Journal, 30, 2, 235.
  • Erel, O., 2005: A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 12, 1103-1111.
  • Felgines C, Texier O, Morand C, Manach C, Scalbert A, Régerat F, Remesy C, 2000: Bioavailability of the flavanone naringenin and its glycosides in rats. American Journal of Physiology-Gastrointestinal and Liver Physiology, 279, 6, G1148-G1154.
  • Geyikoglu F, Emir M, Colak S, Koc K, Turkez H, Bakir M, Hosseinigouzdagani M, Cerig S, Keles ON, Ozek NS, 2017: Effect of oleuropein against chemotherapy drug-induced histological changes, oxidative stress, and DNA damages in rat kidney injury. Journal of Food and Drug Analysis, 25, 2, 447-459.
  • Hosseinian S, Rad AK, Mousa-Al-Reza Hadjzadeh NM, Roshan SH, Shafiee S, 2016: The protective effect of nigella sativa against cisplatin-induced nephrotoxicity in rats. Avicenna Journal of Phytomedicine, 6, 1, 44.
  • Huang HC, Nguyen T, Pickett CB, 2002: Phosphorylation of Nrf2 at Ser-40 by protein kinase C regulates antioxidant response element-mediated transcription. Journal of Biological Chemistry, 277, 45, 42769-42774.
  • Ince S, Acaroz DA, Neuwirth O, Demirel HH, Denk B, Kucukkurt I, Turkmen R, 2014: Protective effect of polydatin, a natural precursor of resveratrol, against cisplatin-induced toxicity in rats. Food and Chemical Toxicology, 72, 147-153.
  • Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M, 1999: Quantitation of flavonoid constituents in citrus fruits. Journal of Agricultural and Food Chemistry, 47, 9, 3565-3571.
  • Kilic U, Sahin K, Tuzcu M, Basak N, Orhan C, Elibol-Can B, Kilic E, Sahin F, Kucuk O, 2015: Enhancement of cisplatin sensitivity in human cervical cancer: epigallocatechin-3-gallate. Frontiers in Nutrition, 1, 28.
  • Lee JM., Li J, Johnson DA, Stein TD, Kraft AD, Calkins MJ, Jakel RJ, Johnson JA, 2005: Nrf2, a multi-organ protector. The Faseb Journal, 19, 9, 1061-1066.
  • Ohkawa H, Ohishi N, Yagi K, 1979: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95, 2, 351-358.
  • Ozkol HU, Koyuncu I, Tuluce Y, Dilsiz N, Soral S, Ozkol H, 2015: Anthocyanin-rich extract from Hibiscus sabdariffa calyx counteracts UVC-caused impairments in rats. Pharmaceutical Biology, 53, 10, 1435-1441.
  • Ozkol HU, Musa D, Tuluce Y, Koyuncu I, 2012: Ameliorative influence of Urtica dioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug and Chemical Toxicology, 35, 3, 251-257.
  • Raza S, Khan M, Ahmad A, Ashafaq M, Islam F, Wagner A, Safhi M, 2013: Neuroprotective effect of naringenin is mediated through suppression of NF-κB signaling pathway in experimental stroke. Neuroscience, 230, 157-171.
  • Sies H, Masumoto H, 1996: Ebselen as a glutathione peroxidase mimic and as a scavenger of peroxynitrite. Advances in Pharmacology Elsevier. 38: 229-246.
  • Soliman AM, Desouky S, Marzouk M, Sayed AA, 2016: Origanum majorana attenuates nephrotoxicity of cisplatin anticancer drug through ameliorating oxidative stress. Nutrients, 8, 5, 264.
  • Topcu-Tarladacalisir Y, Sapmaz-Metin M, Karaca T, 2016: Curcumin counteracts cisplatin-induced nephrotoxicity by preventing renal tubular cell apoptosis. Renal Failure, 38, 10, 1741-1748.
  • Wang J, Gu BJ, Masters CL, Wang YJ, 2017: A systemic view of Alzheimer disease - insights from amyloid-beta metabolism beyond the brain. Nat Rev Neurol, 13, 10, 612-623.
  • Witko V, Nguyen AT, Descamps-Latscha B, 1992: Microtiter plate assay for phagocyte-derived Taurine-chloramines. Journal of Clinical Laboratory Analysis, 6, 1, 47-53.
  • Wozniak, K., A. Czechowska and J. Blasiak, 2004: Cisplatin-evoked DNA fragmentation in normal and cancer cells and its modulation by free radical scavengers and the tyrosine kinase inhibitor STI571. Chemico-biological interactions, 147, 3, 309-318.
  • Xiao Y, Sun H, Li C, Li Y, Peng S, Fan C, Teng W, Shan Z, 2018: Effect of iodine nutrition on pregnancy outcomes in an iodine-sufficient area in China. Biological Trace Element Research, 182, 2, 231-237.
  • Yousef M, Saad A, El-Shennawy L, 2009: Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food and Chemical Toxicology, 47, 6, 1176-1183.

Investigation of the Protective Effect of Naringenin on the Cisplatin-Induced Nephrotoxicity in Rats

Yıl 2018, Cilt: 7 Sayı: 1, 119 - 125, 04.07.2018
https://doi.org/10.31196/huvfd.470988

Öz

Naringenin
which have a bioactive effect on human health is a natural flavonone predominantly
found in grapefruit.
In this study, the effects
of naringenin on some biochemical parameters in renal tissue in
cisplatin-induced nephrotoxicity case were investigated in rats.
In this study 35 Wistar albino rats, aged 2
months were used. Rats were randomly divided into 5 groups each consisted of 7
rats,; 1.group (control) 1 % DMSO i.p, 2.group 
(Cis), one dose cisplatin., 7 mg/kg/ i.p, 3.group (NG20) naringenin, 20
mg/kg/ day /i.p, 4.group, (Cis+NG20 ) one dose cisplatin 7 mg/kg/
i.p + 20 mg/kg/day./i.p naringenin, 5.group (Cis+NG40) one dose cisplatin
7 mg/kg/ i.p + 40 mg/kg/day./i.p naringenin were administered for ten days. At
the end of the study biochemical analyses were made on samples of kidney tissues.
Kidney tissue levels of TOS, OSI, MDA, AOPP, 8-OHdG ve NRF-2 were increased
(P<0.05) and TAS and GSH levels (P<0.05) were decreased significantly in
cisplatin group (2. group) compared with control group. As naringenin
significantly reduced nephrotoxicity in cisplatin-induced rats and nephrotoxicity
could be controlled with naringenin.

Kaynakça

  • Ali BH, Al Moundhri M, 2006: Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food and Chemical Toxicology, 44, 8, 1173-1183.
  • Arul D, Subramanian P, 2013: Inhibitory effect of naringenin (citrus flavonone) on N-nitrosodiethylamine induced hepatocarcinogenesis in rats. Biochemical and Biophysical Research Communications, 434, 2, 203-209.
  • Cadet J, Douki T, Gasparutto D, Ravanat J, 2003: Oxidative damage to DNA: formation, measurement and biochemical features. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 531, 1, 5-23.
  • Coşkun NM, Hatipoğlu T, Özoğul C, Korkmaz C, Akyol SN, Mıcılı SC, Arık GS, Erdoğan D, 2013: The protective effects of acetyl L-carnitine on testis gonadotoxicity induced by cisplatin in rats. Balkan Medical Journal, 30, 2, 235.
  • Erel, O., 2005: A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 12, 1103-1111.
  • Felgines C, Texier O, Morand C, Manach C, Scalbert A, Régerat F, Remesy C, 2000: Bioavailability of the flavanone naringenin and its glycosides in rats. American Journal of Physiology-Gastrointestinal and Liver Physiology, 279, 6, G1148-G1154.
  • Geyikoglu F, Emir M, Colak S, Koc K, Turkez H, Bakir M, Hosseinigouzdagani M, Cerig S, Keles ON, Ozek NS, 2017: Effect of oleuropein against chemotherapy drug-induced histological changes, oxidative stress, and DNA damages in rat kidney injury. Journal of Food and Drug Analysis, 25, 2, 447-459.
  • Hosseinian S, Rad AK, Mousa-Al-Reza Hadjzadeh NM, Roshan SH, Shafiee S, 2016: The protective effect of nigella sativa against cisplatin-induced nephrotoxicity in rats. Avicenna Journal of Phytomedicine, 6, 1, 44.
  • Huang HC, Nguyen T, Pickett CB, 2002: Phosphorylation of Nrf2 at Ser-40 by protein kinase C regulates antioxidant response element-mediated transcription. Journal of Biological Chemistry, 277, 45, 42769-42774.
  • Ince S, Acaroz DA, Neuwirth O, Demirel HH, Denk B, Kucukkurt I, Turkmen R, 2014: Protective effect of polydatin, a natural precursor of resveratrol, against cisplatin-induced toxicity in rats. Food and Chemical Toxicology, 72, 147-153.
  • Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M, 1999: Quantitation of flavonoid constituents in citrus fruits. Journal of Agricultural and Food Chemistry, 47, 9, 3565-3571.
  • Kilic U, Sahin K, Tuzcu M, Basak N, Orhan C, Elibol-Can B, Kilic E, Sahin F, Kucuk O, 2015: Enhancement of cisplatin sensitivity in human cervical cancer: epigallocatechin-3-gallate. Frontiers in Nutrition, 1, 28.
  • Lee JM., Li J, Johnson DA, Stein TD, Kraft AD, Calkins MJ, Jakel RJ, Johnson JA, 2005: Nrf2, a multi-organ protector. The Faseb Journal, 19, 9, 1061-1066.
  • Ohkawa H, Ohishi N, Yagi K, 1979: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95, 2, 351-358.
  • Ozkol HU, Koyuncu I, Tuluce Y, Dilsiz N, Soral S, Ozkol H, 2015: Anthocyanin-rich extract from Hibiscus sabdariffa calyx counteracts UVC-caused impairments in rats. Pharmaceutical Biology, 53, 10, 1435-1441.
  • Ozkol HU, Musa D, Tuluce Y, Koyuncu I, 2012: Ameliorative influence of Urtica dioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug and Chemical Toxicology, 35, 3, 251-257.
  • Raza S, Khan M, Ahmad A, Ashafaq M, Islam F, Wagner A, Safhi M, 2013: Neuroprotective effect of naringenin is mediated through suppression of NF-κB signaling pathway in experimental stroke. Neuroscience, 230, 157-171.
  • Sies H, Masumoto H, 1996: Ebselen as a glutathione peroxidase mimic and as a scavenger of peroxynitrite. Advances in Pharmacology Elsevier. 38: 229-246.
  • Soliman AM, Desouky S, Marzouk M, Sayed AA, 2016: Origanum majorana attenuates nephrotoxicity of cisplatin anticancer drug through ameliorating oxidative stress. Nutrients, 8, 5, 264.
  • Topcu-Tarladacalisir Y, Sapmaz-Metin M, Karaca T, 2016: Curcumin counteracts cisplatin-induced nephrotoxicity by preventing renal tubular cell apoptosis. Renal Failure, 38, 10, 1741-1748.
  • Wang J, Gu BJ, Masters CL, Wang YJ, 2017: A systemic view of Alzheimer disease - insights from amyloid-beta metabolism beyond the brain. Nat Rev Neurol, 13, 10, 612-623.
  • Witko V, Nguyen AT, Descamps-Latscha B, 1992: Microtiter plate assay for phagocyte-derived Taurine-chloramines. Journal of Clinical Laboratory Analysis, 6, 1, 47-53.
  • Wozniak, K., A. Czechowska and J. Blasiak, 2004: Cisplatin-evoked DNA fragmentation in normal and cancer cells and its modulation by free radical scavengers and the tyrosine kinase inhibitor STI571. Chemico-biological interactions, 147, 3, 309-318.
  • Xiao Y, Sun H, Li C, Li Y, Peng S, Fan C, Teng W, Shan Z, 2018: Effect of iodine nutrition on pregnancy outcomes in an iodine-sufficient area in China. Biological Trace Element Research, 182, 2, 231-237.
  • Yousef M, Saad A, El-Shennawy L, 2009: Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food and Chemical Toxicology, 47, 6, 1176-1183.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştıma
Yazarlar

İsmail Koyuncu

Yayımlanma Tarihi 4 Temmuz 2018
Gönderilme Tarihi 1 Mayıs 2018
Kabul Tarihi 7 Haziran 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 7 Sayı: 1

Kaynak Göster

APA Koyuncu, İ. (2018). Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi. Harran Üniversitesi Veteriner Fakültesi Dergisi, 7(1), 119-125. https://doi.org/10.31196/huvfd.470988
AMA Koyuncu İ. Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi. Harran Univ Vet Fak Derg. Temmuz 2018;7(1):119-125. doi:10.31196/huvfd.470988
Chicago Koyuncu, İsmail. “Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi”. Harran Üniversitesi Veteriner Fakültesi Dergisi 7, sy. 1 (Temmuz 2018): 119-25. https://doi.org/10.31196/huvfd.470988.
EndNote Koyuncu İ (01 Temmuz 2018) Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi. Harran Üniversitesi Veteriner Fakültesi Dergisi 7 1 119–125.
IEEE İ. Koyuncu, “Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi”, Harran Univ Vet Fak Derg, c. 7, sy. 1, ss. 119–125, 2018, doi: 10.31196/huvfd.470988.
ISNAD Koyuncu, İsmail. “Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi”. Harran Üniversitesi Veteriner Fakültesi Dergisi 7/1 (Temmuz 2018), 119-125. https://doi.org/10.31196/huvfd.470988.
JAMA Koyuncu İ. Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi. Harran Univ Vet Fak Derg. 2018;7:119–125.
MLA Koyuncu, İsmail. “Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi”. Harran Üniversitesi Veteriner Fakültesi Dergisi, c. 7, sy. 1, 2018, ss. 119-25, doi:10.31196/huvfd.470988.
Vancouver Koyuncu İ. Ratlarda Sisplatin Kaynaklı Nefrotoksisite Üzerine Naringeninin Koruyucu Etkisinin İncelenmesi. Harran Univ Vet Fak Derg. 2018;7(1):119-25.