Alfa-proteobacteria Grubuna Ait Bazı Bakteri Cinslerinden İzole edilen antijenlerin Brusellozisin Serolojik Tanısında Potansiyel Uygulanabilirliğinin Araştırılması
Öz
Brusellozis insanlarda ve hayvanlarda önemli enfeksiyonlara neden olan, halk sağlığını tehdit eden ve hayvancılık endüstrisine ciddi ekonomik kayıplara neden olan zoonoz bir enfeksiyondur. Bu çalışmada, Brucella suşları ile filogenetik yakınlığı bulunan alfa–Proteobacteria sınıfına ait Ochrobactrum anthropi, O. intermedium ve Rhizobium tropici türlerinden hazırlanan antijenlerin brusellosizin serolojik tanısında Brucella antijeni yerine kullanılıp kullanılamayacağının ortaya konulması amaçlandı. Çalışmada, test serumlarına pozitif ve negatif kontroller ile birlikte indirekt ELISA uygulandı. Sonuç olarak, sığır ve insan brusellozisi açısından test edilen antijenlerin hiçbiri güvenilir düzeyde sensitivite ve spesifisite göstermedi. Çalışmada, sadece R-LPS taşıyan O. intermedium antijeni ile ve sadece koyun ve köpeklerde kısmen kabul edilebilir sınırlarda bir tanısal performans alındı. Sonuç olarak, proteobakterilerin α-2 alt grubunda yer alan mikroorganizmaların brusellozisin indirekt teşhisinde bazı çapraz reaksiyonlara neden olabileceği ve bu durumun yanlış pozitiflik yaratabileceği saptandı. Öte yandan bu grup bakterilerden hazırlanan daha saf antijenlerin, B. canis ve B. ovis infeksiyonlarında kullanılma potansiyeli bulunabileceği ve bu yönde yapılacak çalışmalara ihtiyaç olduğu kanısına varıldı.
Anahtar Kelimeler
Destekleyen Kurum
Harran Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü
Proje Numarası
16148
Teşekkür
Bu çalışma, Harran Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından 16148 proje numarası ile Yüksek lisans tezi olarak desteklenmiştir. Destekleri için kuruma teşekkür ederiz.
Kaynakça
- Aras Z, Uçan US, 2008: Brucella abortus ve Brucella melitensis Enfeksiyonlarında Oluşan Antikorların Rhizobium tropici Antijeni ile Tesbit Edilmesi. Vet Bil Derg, 24(1), 47-52.
- Cloeckaert A, Tibor A, Zygmunt SM, 1999: Brucella outer membrane lipoproteins Share antigenic determinants with bacteria of the family Rhizobiaceae. Clin Diagn Lab Immuno, 6, 627-629.
- Da Costa M, Guillou JP, Garin-Bastuji B, Thiébaud M, Dubray G, 1996: Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol, 81, 267-275.
- Delpino MV, Fossati CA, Baldi PC, 2004: Occurrence and Potential Diagnostic Applications of Serological Cross-Reactivities between Brucella and Other Alpha Proteobacteria. Clin Diagn Lab Immunol, 11(5), 868–873.
- Ducrotoy MJ, Munoz PM, Conde-Alvarez R, Blasco JM, Moriyon I, 2018: A systematic review of current immunological tests fort he diagnosis of cattle brucellosis. Prev Vet Med, 151, 57-72.
- Holmes B, Popoff M, Kiredjian M, Kersters K, 1998: Ochrobactrum anthropi gen. nov. from human clinical specimens and previously known as Group Vd. Int J of Syst Bacteriol, 38,406-416.
- Velasco J, Diaz R, Grilló MJ, Barberán M, Marín C, Blasco JM and Moriyón I,v1997: Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp. Clin Diagn Lab Immunol, 4, 279–284.
- Velasco J, Romero C, López-Goñi I, Leiva J, Díaz R, Moriyón I, 1998: Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov. a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol, 48,759–768.
- Vila A, Pagella H, Bello GV, Vicente A, 2016: Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system. J Infect Dev Ctries, 10(4), 432-436.
- Vizcaino N, Cloeckaert A, Verger J, Grayon M, Fernandez-Lago L, 2000: DNA polymorphism in the genus Brucella. Microb Infect, 2(9),1089-1100.
- Yi EC, Hackett M, 2000: Rapid isolation method for lipopolysaccharide and lipid A from gram negative bacteria. Analyst, 125(4), 651-6.
- Xu J, Moore JE, Millar BC, Crowe M, McClurg R, Heaney LG, 2003: Identification of a novel alphaproteobacterium causing bacteremia in immuncompetent patient. J Infect, 47,167-169.
Evaluation of Potential Usage of Antigens Prepared from Some Bacterial Genera of Alpha-Proteobacteria in Serologic Diagnosis of Brucellosis
Öz
Brusellosis is an important zoonosis that cause serious infections in man and animals. This infection constitutes a risk for public health and big economical losses in animal industry. Serological tests have been intensively used for serologic diagnosis of brucellosis because these tests are quicker and more practical than culturing of the causative organisms. Antigens used in serological tests are prepared from virulent Brucella strains. In this regard, to use antigens prepared from bacterial strains that are not virulent for human and genetically close to Brucella spp. in the serological dagnosis of the disease will be a desired approach. These organisms have much lower virulence than Brucella and generally they are considered as saphrophytic bacteria and some of them are oppurtunistic pathogen like O. anthropi. In this study, we planned to search the possibility of usage of antigens prepared from three bacterial species of alpha-Proteobacteria (Ochrobactrum anthropi, O intermedium, Rhizobium tropici) which are filogenetically close to Brucella. As conclusion, none of the antigens yielded acceptable sensitivity and specificity for cattle and human brucellosis. Only sheep and dogs sera against O. intermedium, which is a rough species and carry R-LPS instead of S-LPS, showed diagnostic performance in acceptable ranges. When considering all the results of the study, it was thought that some members of alpha Proteobacteria might cause cross reaction in the serologic diagnosis of brucellosis and cause false positive reactions and this should be remembered when evaluating the serologic diagnosis of brucellosis. On the other hand, pure antigens from O. intermedium might have the potential to be used in B. ovis and B. canis infections and it was thought that the future works in this field might be needed.
Proje Numarası
16148
Kaynakça
- Aras Z, Uçan US, 2008: Brucella abortus ve Brucella melitensis Enfeksiyonlarında Oluşan Antikorların Rhizobium tropici Antijeni ile Tesbit Edilmesi. Vet Bil Derg, 24(1), 47-52.
- Cloeckaert A, Tibor A, Zygmunt SM, 1999: Brucella outer membrane lipoproteins Share antigenic determinants with bacteria of the family Rhizobiaceae. Clin Diagn Lab Immuno, 6, 627-629.
- Da Costa M, Guillou JP, Garin-Bastuji B, Thiébaud M, Dubray G, 1996: Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol, 81, 267-275.
- Delpino MV, Fossati CA, Baldi PC, 2004: Occurrence and Potential Diagnostic Applications of Serological Cross-Reactivities between Brucella and Other Alpha Proteobacteria. Clin Diagn Lab Immunol, 11(5), 868–873.
- Ducrotoy MJ, Munoz PM, Conde-Alvarez R, Blasco JM, Moriyon I, 2018: A systematic review of current immunological tests fort he diagnosis of cattle brucellosis. Prev Vet Med, 151, 57-72.
- Holmes B, Popoff M, Kiredjian M, Kersters K, 1998: Ochrobactrum anthropi gen. nov. from human clinical specimens and previously known as Group Vd. Int J of Syst Bacteriol, 38,406-416.
- Velasco J, Diaz R, Grilló MJ, Barberán M, Marín C, Blasco JM and Moriyón I,v1997: Antibody and delayed-type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp. Clin Diagn Lab Immunol, 4, 279–284.
- Velasco J, Romero C, López-Goñi I, Leiva J, Díaz R, Moriyón I, 1998: Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov. a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol, 48,759–768.
- Vila A, Pagella H, Bello GV, Vicente A, 2016: Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system. J Infect Dev Ctries, 10(4), 432-436.
- Vizcaino N, Cloeckaert A, Verger J, Grayon M, Fernandez-Lago L, 2000: DNA polymorphism in the genus Brucella. Microb Infect, 2(9),1089-1100.
- Yi EC, Hackett M, 2000: Rapid isolation method for lipopolysaccharide and lipid A from gram negative bacteria. Analyst, 125(4), 651-6.
- Xu J, Moore JE, Millar BC, Crowe M, McClurg R, Heaney LG, 2003: Identification of a novel alphaproteobacterium causing bacteremia in immuncompetent patient. J Infect, 47,167-169.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştıma |
| Yazarlar | |
| Proje Numarası | 16148 |
| Yayımlanma Tarihi | 22 Haziran 2021 |
| Gönderilme Tarihi | 9 Şubat 2021 |
| Kabul Tarihi | 31 Mayıs 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 10 Sayı: 1 |
