Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Identification of meat species in different types of meat products by PCR

Yıl 2017, Cilt: 64 Sayı: 4, 261 - 266, 01.12.2017

Erhan KEYVAN Güzin ÇİL İPLİKÇİOĞLU Bengi KUL ÇINAR Nüket BİLGEN Ufuk Tansel ŞİRELİ

https://doi.org/10.1501/Vetfak_0000002818

Öz

Species identification in food of animal origin is an important subject for food control. Adulteration in foods is relevant for economical, religous, legislation or public health concerning reasons. The aim of this study was to determine the adulteration in different meat products such as sucuk (n=37), sausage (n=33) and salami (n=32). A total of 102 different meat products obtained from various markets in Ankara were analyzed for species identification by PCR. According to the analyzes, five (13.5%) poultry, and one (2.7%) poultry and equine meats together were found in the sucuk samples that are not declared in their labels. Also, seven (21.8%) and two (6.1%) poultry meat were detected in 32 salami and 33 sausage samples, respectively. These results indicate that 15 (14.7%) of the total samples were found to contain undeclared species. As a result, there were meat products which were not in compliance with their labels in various markets, presenting a potential public health risk and economical losses of consumers

Anahtar Kelimeler

Adulteration, meat species identification, mislabeling, PCR

Kaynakça

  • Anonymous (2014): Livestock Production in Turkey. Turkish Statistical Institute.
  • Anonymous. Turkish Food Codex. Communique on Meat and Meat Products. 2012/74.
  • Ayaz Y, Ayaz ND, Erol I (2006): Detection of species in meat immunosorbent assay. J Muscle Foods, 17, 214-220.
  • Ballin NZ (2010): Authentication of meat and meat products. Meat Sci, 86, 577-587.
  • Camm C, Di Domenico M, Monaco F (2012): Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures. Food Control, 23, 400-404.
  • Cawthorn D, Steinman HA, Hoffman LC (2013): A high incidence of species substitution and mislabelling detected in meat products sold in South Africa. Food Control, 32, 440-449.
  • Colombo F, Marchisio E, Pizzini A, et al. (2002): Identification of the goose species (Anseranser) in Italian “Mortara” salami by DNA sequencing and a polymerase chain reaction with an original primer pair. Meat Sci, 61, 291-294.
  • Dalmasso A, Fontanella E, Piatti P, et al. (2004): A multiplex PCR assay for the identification of animal species in feedstuffs. Mol Cell Probes, 18, 81-87.
  • Dooley JJ, Paine KE, Garrett SD, et al. (2004): Detection of meat species using TaqMan real-time PCR assays. Meat Sci, 68, 431-438.
  • Ghovvati S, Nassiri MR, Mirhoseini SZ, et al. (2009): Fraud identification in industrial meat products by multiplex PCR assay. Food Control, 20, 696-699.
  • Günşen U, Aydın A, Ovalı BB, et al. (2006): Çiğ et ve ısıl işlem görmüş et ürünlerinde ELISA tekniği ile farklı et türlerinin tespiti. J Vet Med Istanbul Univ, 32, 45-52.
  • Hsieh YP, Johnson MA, Wetzstein CJ, et al. (1996): Detection of species adulteration in pork products using agar-gel immunosorbent assay. J Food Quality, 19, 1-9. and enzyme-linked
  • Hsieh YP, Woodward BB, Ho SH (1995): Detection of species substitution in rawand cooked meats using immunoassays. J Food Protec, 58, 555-559.
  • Kesmen Z, Gulluce A, Sahin F, et al. (2009): Identification of meat species by TaqMan-based real-time PCR assay. Meat Sci, 82, 444-449.
  • Lenstra JA (2003): DNA Methods for Identifying Plant and Animal Species in Food. 34-54. In: Lees M (Ed), Food Authenticity and Traceability. CRC Press, New York.
  • Lockley AK, Bardsley RG (2000): DNA-based methods for food authentication. Trends Food Sci Technol, 1, 67-77.
  • Mafra I, Beatriz P, Oliveira P (2008): Food authentication by PCR-based methods. European Food Res Technol, 227, 649-665.
  • Mane BG, Mendiratta SK, Tiwari AK (2009): Polymerase chain reaction assay for identification of chicken in meat and meat products. Food Chem, 116, 806- 810.
  • Martín I, García T, Fajardo V, et al. (2007): Species- specific PCR for the identification of ruminant species in feedstuffs. Meat Sci, 75, 120-127.
  • Martinez I, Yman IM (1998): Species identification in meat products by RAPD analysis. Food Res Int, 31, 459-466.
  • Nakyinsige K, Man YB, Sazili AQ (2012): Halal authenticity issues in meat and meat products. Meat Sci, 91, 207-214.
  • Saez R, Sanz Y, Toldrá F (2004): PCR-based fingerprinting techniques for rapid detection of animal species in meat products. Meat Sci, 66, 659-665.
  • Spink J, Moyer DC (2013): Understanding and combating food fraud. Food Technol, 67, 30-35.
  • Türkyılmaz Ö, Irmak H (2008): Et ve et ürünlerinde ELISA tekniği ile türlerin tespiti. J Bornova Vet Sci, 30, 27-31.
  • Ulca P, Balta H, Çağın I, et al. (2013): Meat species identification and Halal authentication using PCR analysis of raw and cooked traditional Turkish foods. Meat Sci, 94, 280-284.
  • Verkaar ELC, Nijman IJ, Boutaga K, et al. (2002): Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA. Meat Sci, 60, 365-369.

Farklı tip et ürünlerinde PZR ile tür tayini

Yıl 2017, Cilt: 64 Sayı: 4, 261 - 266, 01.12.2017

Erhan KEYVAN Güzin ÇİL İPLİKÇİOĞLU Bengi KUL ÇINAR Nüket BİLGEN Ufuk Tansel ŞİRELİ

https://doi.org/10.1501/Vetfak_0000002818

Öz

Et ürünlerinde tür tayini gıda güvenliği açısından önemli bir konudur. Etikette bildirilmeyen çeşitli hayvan türlerine ait etlerin kullanımı, tüketici sağlığı, ülke ekonomisi, dini ve yasal düzenlemeler ile ilişkilendirilmektedir. Bu çalışmada, sucuk, salam, sosis gibi ürünlerde farklı türlere ait etlerin varlığının tespiti amaçlanmıştır. Araştırmada Ankara’da çeşitli marketlerde satışa sunulan toplam 102 adet işlenmiş et ürünü tür tayini amacıyla PZR yöntemi kullanılarak incelenmiştir. Yapılan analizler sonucunda, incelenen 37 adet sucuk örneğinden etikette belirtilmeyen 5 (%13.5) kanatlı, 1 (%2.7) kanatlı ve tek tırnaklı eti tespit edilmiştir. Benzer şeklide, 32 salam örneğinin yedisinde (%21.8), 33 adet sosis örneğinin de ikisinde (%6.1) kanatlı eti bulunmuştur. Ürünlerin etiket bilgileri karşılaştırıldığında 15 adet (% 14.7) örneğin etiketinde belirtilmeyen farklı hayvan türlerine ait etlerden üretildiği tespit edilmiştir. Sonuç olarak, Ankara’da farklı marketlerden alınan işlenmiş et ürünleri örneklerinde, etiketlerinde belirtilmeyen hayvan türlerine ait etlerin kullanıldığı ve bu durumun potansiyel halk sağlığı sorunları yanında tüketicinin ekonomik olarak da kayba uğramasına sebep olabileceği ortaya konmuştur

Anahtar Kelimeler

Hile, et tür tayini, yanlış etiketleme, PZR

Kaynakça

  • Anonymous (2014): Livestock Production in Turkey. Turkish Statistical Institute.
  • Anonymous. Turkish Food Codex. Communique on Meat and Meat Products. 2012/74.
  • Ayaz Y, Ayaz ND, Erol I (2006): Detection of species in meat immunosorbent assay. J Muscle Foods, 17, 214-220.
  • Ballin NZ (2010): Authentication of meat and meat products. Meat Sci, 86, 577-587.
  • Camm C, Di Domenico M, Monaco F (2012): Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures. Food Control, 23, 400-404.
  • Cawthorn D, Steinman HA, Hoffman LC (2013): A high incidence of species substitution and mislabelling detected in meat products sold in South Africa. Food Control, 32, 440-449.
  • Colombo F, Marchisio E, Pizzini A, et al. (2002): Identification of the goose species (Anseranser) in Italian “Mortara” salami by DNA sequencing and a polymerase chain reaction with an original primer pair. Meat Sci, 61, 291-294.
  • Dalmasso A, Fontanella E, Piatti P, et al. (2004): A multiplex PCR assay for the identification of animal species in feedstuffs. Mol Cell Probes, 18, 81-87.
  • Dooley JJ, Paine KE, Garrett SD, et al. (2004): Detection of meat species using TaqMan real-time PCR assays. Meat Sci, 68, 431-438.
  • Ghovvati S, Nassiri MR, Mirhoseini SZ, et al. (2009): Fraud identification in industrial meat products by multiplex PCR assay. Food Control, 20, 696-699.
  • Günşen U, Aydın A, Ovalı BB, et al. (2006): Çiğ et ve ısıl işlem görmüş et ürünlerinde ELISA tekniği ile farklı et türlerinin tespiti. J Vet Med Istanbul Univ, 32, 45-52.
  • Hsieh YP, Johnson MA, Wetzstein CJ, et al. (1996): Detection of species adulteration in pork products using agar-gel immunosorbent assay. J Food Quality, 19, 1-9. and enzyme-linked
  • Hsieh YP, Woodward BB, Ho SH (1995): Detection of species substitution in rawand cooked meats using immunoassays. J Food Protec, 58, 555-559.
  • Kesmen Z, Gulluce A, Sahin F, et al. (2009): Identification of meat species by TaqMan-based real-time PCR assay. Meat Sci, 82, 444-449.
  • Lenstra JA (2003): DNA Methods for Identifying Plant and Animal Species in Food. 34-54. In: Lees M (Ed), Food Authenticity and Traceability. CRC Press, New York.
  • Lockley AK, Bardsley RG (2000): DNA-based methods for food authentication. Trends Food Sci Technol, 1, 67-77.
  • Mafra I, Beatriz P, Oliveira P (2008): Food authentication by PCR-based methods. European Food Res Technol, 227, 649-665.
  • Mane BG, Mendiratta SK, Tiwari AK (2009): Polymerase chain reaction assay for identification of chicken in meat and meat products. Food Chem, 116, 806- 810.
  • Martín I, García T, Fajardo V, et al. (2007): Species- specific PCR for the identification of ruminant species in feedstuffs. Meat Sci, 75, 120-127.
  • Martinez I, Yman IM (1998): Species identification in meat products by RAPD analysis. Food Res Int, 31, 459-466.
  • Nakyinsige K, Man YB, Sazili AQ (2012): Halal authenticity issues in meat and meat products. Meat Sci, 91, 207-214.
  • Saez R, Sanz Y, Toldrá F (2004): PCR-based fingerprinting techniques for rapid detection of animal species in meat products. Meat Sci, 66, 659-665.
  • Spink J, Moyer DC (2013): Understanding and combating food fraud. Food Technol, 67, 30-35.
  • Türkyılmaz Ö, Irmak H (2008): Et ve et ürünlerinde ELISA tekniği ile türlerin tespiti. J Bornova Vet Sci, 30, 27-31.
  • Ulca P, Balta H, Çağın I, et al. (2013): Meat species identification and Halal authentication using PCR analysis of raw and cooked traditional Turkish foods. Meat Sci, 94, 280-284.
  • Verkaar ELC, Nijman IJ, Boutaga K, et al. (2002): Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA. Meat Sci, 60, 365-369.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA29DF45NE
Bölüm Araştırma Makalesi
Yazarlar

Erhan KEYVAN
Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Burdur;

Güzin ÇİL İPLİKÇİOĞLU
Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Burdur;

Bengi KUL ÇINAR
Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Burdur;

Nüket BİLGEN
Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Burdur;

Ufuk Tansel ŞİRELİ
Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Burdur;

Yayımlanma Tarihi 1 Aralık 2017
Yayınlandığı Sayı Yıl 2017Cilt: 64 Sayı: 4

Kaynak Göster

Bibtex @editöre mektup { auvfd525935, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2017}, volume = {64}, number = {4}, pages = {261 - 266}, doi = {10.1501/Vetfak\_0000002818}, title = {Identification of meat species in different types of meat products by PCR}, key = {cite}, author = {Keyvan, Erhan and İplikçioğlu, Güzin ÇİL and Çınar, Bengi KUL and Bilgen, Nüket and Şireli, Ufuk Tansel} }
APA Keyvan, E. , İplikçioğlu, G. Ç. , Çınar, B. K. , Bilgen, N. & Şireli, U. T. (2017). Identification of meat species in different types of meat products by PCR . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 64 (4) , 261-266 . DOI: 10.1501/Vetfak_0000002818
MLA Keyvan, E. , İplikçioğlu, G. Ç. , Çınar, B. K. , Bilgen, N. , Şireli, U. T. "Identification of meat species in different types of meat products by PCR" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 261-266 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43284/525935>
Chicago Keyvan, E. , İplikçioğlu, G. Ç. , Çınar, B. K. , Bilgen, N. , Şireli, U. T. "Identification of meat species in different types of meat products by PCR". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 261-266
RIS TY - JOUR T1 - Farklı tip et ürünlerinde PZR ile tür tayini AU - ErhanKeyvan, Güzin ÇİLİplikçioğlu, Bengi KULÇınar, NüketBilgen, Ufuk TanselŞireli Y1 - 2017 PY - 2017 N1 - doi: 10.1501/Vetfak_0000002818 DO - 10.1501/Vetfak_0000002818 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 261 EP - 266 VL - 64 IS - 4 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002818 UR - https://doi.org/10.1501/Vetfak_0000002818 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Identification of meat species in different types of meat products by PCR %A Erhan Keyvan , Güzin ÇİL İplikçioğlu , Bengi KUL Çınar , Nüket Bilgen , Ufuk Tansel Şireli %T Identification of meat species in different types of meat products by PCR %D 2017 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 64 %N 4 %R doi: 10.1501/Vetfak_0000002818 %U 10.1501/Vetfak_0000002818
ISNAD Keyvan, Erhan , İplikçioğlu, Güzin ÇİL , Çınar, Bengi KUL , Bilgen, Nüket , Şireli, Ufuk Tansel . "Identification of meat species in different types of meat products by PCR". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 / 4 (Aralık 2017): 261-266 . https://doi.org/10.1501/Vetfak_0000002818
AMA Keyvan E. , İplikçioğlu G. Ç. , Çınar B. K. , Bilgen N. , Şireli U. T. Identification of meat species in different types of meat products by PCR. Ankara Univ Vet Fak Derg. 2017; 64(4): 261-266.
Vancouver Keyvan E. , İplikçioğlu G. Ç. , Çınar B. K. , Bilgen N. , Şireli U. T. Identification of meat species in different types of meat products by PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64(4): 261-266.
IEEE E. Keyvan , G. Ç. İplikçioğlu , B. K. Çınar , N. Bilgen ve U. T. Şireli , "Identification of meat species in different types of meat products by PCR", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 64, sayı. 4, ss. 261-266, Ara. 2017, doi:10.1501/Vetfak_0000002818