Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks

Yıl 2017, Cilt: 64 Sayı: 4, 329 - 335, 01.12.2017

Merve Baydin ÖZGÜR Seval Dağalp BİLGE

https://doi.org/10.1501/Vetfak_0000002817

Öz

In this study, we have investigated the seroprevalence of alphaherpesvirus (BoHV-1 and CpHV-1) infections in selected goat flocks using glycoprotein B (gB)/glycoprotein E (gE) ELISA combination and virus neutralisation test (VNT). For this purpose, we collected blood serum samples of 546 Saanen goats from Bolu province and tested them by ELISA and VNT. Using ELISA, 32.05% (175/546) of the samples were found to be gB(+)/gE(-) for CpHV-1, whereas only 0.73% (4/546) of the samples were noted to be gB(+)/gE(+) for BoHV-1 releated infection. By performing VNT, we found 31.86% (174/546) and 3.29% (18/546) positivity for CpHV-1 and BoHV-1, respectively. In conclusion, prevalence of CpHV-1 infection was found to be higher than BoHV1 infection in sampled goat flocks. For diagnosis of alphaherpesviruses in the goats, use of gB blocking ELISA test was found to be favorable as an alternative method. On the other hand, to distinguish between the CpHV-1/BoHV-1, gE blocking ELISA in combination by VNT was found to be incompatible with the statistical analysis (p<0.001). Considering antigenic cross-reactions between these viruses, because of the incompatibility of these tests, using a more sensitive/specific method to determine CpHV-1 and BoHV-1 antibodies can be suggested

Anahtar Kelimeler

Bovine herpesvirus 1, caprine herpesvirus 1, gB/gE ELISA, goat, virus neutralisation test

Kaynakça

  • Alpay G, Tuncer P, Yesilbag K (2014): Serological distribution of some viral infections in cattle, sheep and goats in an isolated islandecosystem. Vet J Ankara Univ, 61, 43-48.
  • Ataseven VS, Basaran Z, Yılmaz V, et al. (2010): Seroprevalence of parainfluenza virus-3 (PIV-3) and bovine herpesvirus type 1 (BHV-1) infections in goats of Van region. YYÜ Veteriner Fak Derg, 21, 7-9.
  • Berrios PE, Mckercher DG (1975): Characterization caprine herpesvirus. Am J Vet Res, 36, 1755-1762.
  • Bertolotti L, Rosamilia A, Profiti M, et al. (2013): Characterization of caprine herpesvirus 1 (CpHV1) glycoprotein E ve glycoprotein I ectodomains expressed in mammalian cells. Vet Microbiol, 164, 222-228.
  • El-Azhary MA, Roy RS, Frechette JL (1979): Serological evidence of IBR and BVD infection in Caribou (Rangifer tarandus). Vet Rec, 105, 336.
  • Frey HR, Liess B (1971): Vermehrungskinetik und Verwendbarkeit einer stark zytopathogenen VD-MD Virusstammes für diagnostische Untersuchungen mit der mikrotiter-Methode. Zentbl Vet Med, 18, 61-71.
  • Fulton RW, Briggs RE, Payton ME, et al. (2004): Maternally derived humoral immunity to bovine viral diarrhea virus (BVDV) 1a, BVDV1b, BVDV2, bovine herpesvirus-1, parainfluenza-3 virus bovine respiratory syncytial virus, Mannheimia haemolytica and Pasteurella multocida in beef calves, antibody decline by half-life studies and effect on response to vaccination. Vaccine, 22, 643-649.
  • Guercio A, Greco G, Lanizzoto G, et al. (1998): Valutazione della diffusione di anticorpi anti Herpes Virus della capra in allevamenti caprini della Sicilia. Atti. SIPAOC, 12, 138-142.
  • Keuser V, Espejo-Serrano J, Schynts F, et al. (2004): Isolation of caprine herpesvirus type 1 in Spain. Vet Rec, 154, 395-399.
  • Koptopoulos G, Papanastasopoulou M, Papadopoulos O, et al. (1988): The epizootology of caprine herpesvirus (BHV-6) infections in goat populations in Greece. Comp Immunol Microb, 11, 199-205.
  • Kramps JA, Banks M, Beer M, et al. (2004): Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe. Vet Microbiol, 102, 169-181.
  • Lyaku JR, Nettleton PF, Mardsen H (1992): A comparison of serological relationships among five ruminant alphaherpesviruses by ELISA. Arch Virol, 124, 333-341.
  • Mahmoud MA, Ahmed SA (2009): Prevalence of bovine herpesvirus-1 in sheep and goats in Egypt. Lobal Veterinaria Giza, 3, 472-479.
  • Marinaro M, Bellacicco AL, Tarsitano E, et al. (2010): Detection of CpHV-1 spesific antibodies in goat sera using an ELISA and SN Test. J Vet Diagn Invest, 22, 245-248.
  • Martin WB, Castrucci G, Frigeri F, et al. (1990): A serological comparison of some animal herpesviruses. Comp Immunol Microbiol Infect Dis, 13, 75-84.
  • Nixon P, Edwards S, White H (1988): Serological comparisons of antigenically related herpesviruses in cattle, red deer and goats. Vet Res Commun, 12, 355-362.
  • Ros C, Belak S (2002): Characterization of the glycoprotein B gene from ruminant alphaherpesviruses. Virus Genes, 24, 99-105.
  • Saito JK, Grıbble DH, Berrios PE, et al. (1974): A new herpesvirus isolate from goats: Preliminary report. Am J Vet Res, 35, 847-848.
  • Silva MLCR, Pituco EM, Nogueira AHC, et al. (2013): Serological evidence and risk factors associated cith caprine herpesvirus 1 in dairy goat flocks in a semiarid region of northeastern Brazil. J Vet Diagn Invest, 25, 125.
  • Taylor J. Herbert (1977): Increase in DNA replication sites in cells held at the beginning of S phase. Chromosoma, 62, 291-300.
  • Tempesta M, Cavalli A, Voigt V, et al. (1994): Presenza di anticorpi per caprine herpesvirus 1 (CapHV.1) in allevamenti caprini dell’Italia meridionale. Atti. SIPAOC, 11, 121-122.
  • Thiry J, Keuser V, Muylkens B, et al. (2006a): Ruminant alphaherpesviruses related to bovine herpesvirus 1. Vet Res, 37, 169-190.
  • Thiry J, Tempesta M, Camero M, et al. (2007): Clinical protection against caprine herpesvirus 1 genital infection by intranasal administration of a live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine. BMC Vet Res, 3, 33.
  • Thiry J, Tempesta M, Camero M, et al. (2006b): A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats. Vet Microbiol, 113, 303- 308.
  • Thiry J, Saegerman C, Chartier C, et al. (2008): Serological evidence of caprine herpesvirus 1 infection in Mediterranean France. Vet Microbiol, 128, 261-268.
  • Yeşilbağ K, Bilge Dağalp S, Okur Gümüşova S, et al. (2003): Studies on Herpesvirus Infections of Goats in Turkey: Prevalence of Antibodies to Bovine herpesvirus 1. Rev Med Vet, 154, 772-774.
  • Yonguç AD, Akçora A, Vural B (1990): Serological and etiological studies on caprine herpesvirus infection. Project No: Tubitak, VHAG-756. Geliş tarihi: 11.05.2016 / Kabul tarihi: 22.10.2016 Adress for correspondence: Merve Özgür Baydın, Veterinary Control Central Research Institute, Ankara, Turkey. e-mail: merveozgur87@gmail.com

Seçilen keçi sürülerinde gB/gE ELISA kombinasyonu ve VNT kullanılarak BoHV-1 ve CpHV-1enfeksiyonlarının seroprevalansının araştırılması

Yıl 2017, Cilt: 64 Sayı: 4, 329 - 335, 01.12.2017

Merve Baydin ÖZGÜR Seval Dağalp BİLGE

https://doi.org/10.1501/Vetfak_0000002817

Öz

Bu çalışmada, seçilmiş keçi sürülerinde BoHV-1 ve CpHV-1 enfeksiyonlarının seroprevalansı glikoprotein B (gB)/ glikoprotein E (gE) ELISA kombinasyonu ve virus nötralizasyon testi (VNT) kullanılarak araştırılmıştır. Bu amaçla, Bolu ilinden 546 Saanen keçisinden alınan kan serumu örnekleri ELISA ve VNT kullanılarak test edilmiştir. Her iki ELISA sistemi ile test edilen örneklerin %32.05’i (175/546) CpHV-1 antikor pozitifliğini yansıtan gB(+)/gE(-) olarak bulunurken, % 0.73’ü (4/546) BoHV-1 antikorları yönünden pozitifliği yansıtan gB(+)/gE(+) olarak belirlenmiştir. VNT ile serumların %31.86’ sı (174/546) CpHV-1, %3.29’u (18/546) BoHV-1 antikorları yönünden pozitif olarak değerlendirilmiştir. Sonuç olarak, örneklenen keçi sürülerinde CpHV1 enfeksiyonu prevalansı, BoHV-1 enfeksiyonundan yüksek bulunmuştur. Keçilerde alphaherpesvirus enfeksiyonlarının tanısında gB blocking ELISA alternatif test olarak kullanılabillir bulunmuş, ancak CpHV-1 ve BoHV-1 ayrımında gE blocking ELISA ve VNT kombinasyonunun kullanılması istatisitiksel (p<0.001) olarak uygun bulunmamıştır. Bu viruslar arasındaki antijenik çaprazreaksiyonları göz önünde bulundurarak, bu testlerdeki uyumsuzluklardan dolayı, CpHV-1 ve BoHV-1 antikorlarının belirlenmesi amacıyla daha hassas ve spesifik metotların geliştirilmesi önerilmektedir

Anahtar Kelimeler

gB/gE ELISA, keçi, keçi herpesvirus 1, sığır herpesvirus 1, virus nötralizasyon testi

Kaynakça

  • Alpay G, Tuncer P, Yesilbag K (2014): Serological distribution of some viral infections in cattle, sheep and goats in an isolated islandecosystem. Vet J Ankara Univ, 61, 43-48.
  • Ataseven VS, Basaran Z, Yılmaz V, et al. (2010): Seroprevalence of parainfluenza virus-3 (PIV-3) and bovine herpesvirus type 1 (BHV-1) infections in goats of Van region. YYÜ Veteriner Fak Derg, 21, 7-9.
  • Berrios PE, Mckercher DG (1975): Characterization caprine herpesvirus. Am J Vet Res, 36, 1755-1762.
  • Bertolotti L, Rosamilia A, Profiti M, et al. (2013): Characterization of caprine herpesvirus 1 (CpHV1) glycoprotein E ve glycoprotein I ectodomains expressed in mammalian cells. Vet Microbiol, 164, 222-228.
  • El-Azhary MA, Roy RS, Frechette JL (1979): Serological evidence of IBR and BVD infection in Caribou (Rangifer tarandus). Vet Rec, 105, 336.
  • Frey HR, Liess B (1971): Vermehrungskinetik und Verwendbarkeit einer stark zytopathogenen VD-MD Virusstammes für diagnostische Untersuchungen mit der mikrotiter-Methode. Zentbl Vet Med, 18, 61-71.
  • Fulton RW, Briggs RE, Payton ME, et al. (2004): Maternally derived humoral immunity to bovine viral diarrhea virus (BVDV) 1a, BVDV1b, BVDV2, bovine herpesvirus-1, parainfluenza-3 virus bovine respiratory syncytial virus, Mannheimia haemolytica and Pasteurella multocida in beef calves, antibody decline by half-life studies and effect on response to vaccination. Vaccine, 22, 643-649.
  • Guercio A, Greco G, Lanizzoto G, et al. (1998): Valutazione della diffusione di anticorpi anti Herpes Virus della capra in allevamenti caprini della Sicilia. Atti. SIPAOC, 12, 138-142.
  • Keuser V, Espejo-Serrano J, Schynts F, et al. (2004): Isolation of caprine herpesvirus type 1 in Spain. Vet Rec, 154, 395-399.
  • Koptopoulos G, Papanastasopoulou M, Papadopoulos O, et al. (1988): The epizootology of caprine herpesvirus (BHV-6) infections in goat populations in Greece. Comp Immunol Microb, 11, 199-205.
  • Kramps JA, Banks M, Beer M, et al. (2004): Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe. Vet Microbiol, 102, 169-181.
  • Lyaku JR, Nettleton PF, Mardsen H (1992): A comparison of serological relationships among five ruminant alphaherpesviruses by ELISA. Arch Virol, 124, 333-341.
  • Mahmoud MA, Ahmed SA (2009): Prevalence of bovine herpesvirus-1 in sheep and goats in Egypt. Lobal Veterinaria Giza, 3, 472-479.
  • Marinaro M, Bellacicco AL, Tarsitano E, et al. (2010): Detection of CpHV-1 spesific antibodies in goat sera using an ELISA and SN Test. J Vet Diagn Invest, 22, 245-248.
  • Martin WB, Castrucci G, Frigeri F, et al. (1990): A serological comparison of some animal herpesviruses. Comp Immunol Microbiol Infect Dis, 13, 75-84.
  • Nixon P, Edwards S, White H (1988): Serological comparisons of antigenically related herpesviruses in cattle, red deer and goats. Vet Res Commun, 12, 355-362.
  • Ros C, Belak S (2002): Characterization of the glycoprotein B gene from ruminant alphaherpesviruses. Virus Genes, 24, 99-105.
  • Saito JK, Grıbble DH, Berrios PE, et al. (1974): A new herpesvirus isolate from goats: Preliminary report. Am J Vet Res, 35, 847-848.
  • Silva MLCR, Pituco EM, Nogueira AHC, et al. (2013): Serological evidence and risk factors associated cith caprine herpesvirus 1 in dairy goat flocks in a semiarid region of northeastern Brazil. J Vet Diagn Invest, 25, 125.
  • Taylor J. Herbert (1977): Increase in DNA replication sites in cells held at the beginning of S phase. Chromosoma, 62, 291-300.
  • Tempesta M, Cavalli A, Voigt V, et al. (1994): Presenza di anticorpi per caprine herpesvirus 1 (CapHV.1) in allevamenti caprini dell’Italia meridionale. Atti. SIPAOC, 11, 121-122.
  • Thiry J, Keuser V, Muylkens B, et al. (2006a): Ruminant alphaherpesviruses related to bovine herpesvirus 1. Vet Res, 37, 169-190.
  • Thiry J, Tempesta M, Camero M, et al. (2007): Clinical protection against caprine herpesvirus 1 genital infection by intranasal administration of a live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine. BMC Vet Res, 3, 33.
  • Thiry J, Tempesta M, Camero M, et al. (2006b): A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats. Vet Microbiol, 113, 303- 308.
  • Thiry J, Saegerman C, Chartier C, et al. (2008): Serological evidence of caprine herpesvirus 1 infection in Mediterranean France. Vet Microbiol, 128, 261-268.
  • Yeşilbağ K, Bilge Dağalp S, Okur Gümüşova S, et al. (2003): Studies on Herpesvirus Infections of Goats in Turkey: Prevalence of Antibodies to Bovine herpesvirus 1. Rev Med Vet, 154, 772-774.
  • Yonguç AD, Akçora A, Vural B (1990): Serological and etiological studies on caprine herpesvirus infection. Project No: Tubitak, VHAG-756. Geliş tarihi: 11.05.2016 / Kabul tarihi: 22.10.2016 Adress for correspondence: Merve Özgür Baydın, Veterinary Control Central Research Institute, Ankara, Turkey. e-mail: merveozgur87@gmail.com

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA47DT27NT
Bölüm Araştırma Makalesi
Yazarlar

Merve Baydin ÖZGÜR
Etlik Veterinary Control Central Research Institute

Seval Dağalp BİLGE
Etlik Veterinary Control Central Research Institute

Yayımlanma Tarihi 1 Aralık 2017
Yayınlandığı Sayı Yıl 2017Cilt: 64 Sayı: 4

Kaynak Göster

Bibtex @editöre mektup { auvfd525962, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2017}, volume = {64}, number = {4}, pages = {329 - 335}, doi = {10.1501/Vetfak\_0000002817}, title = {Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks}, key = {cite}, author = {Özgür, Merve Baydin and Bilge, Seval Dağalp} }
APA Özgür, M. B. & Bilge, S. D. (2017). Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 64 (4) , 329-335 . DOI: 10.1501/Vetfak_0000002817
MLA Özgür, M. B. , Bilge, S. D. "Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 329-335 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43284/525962>
Chicago Özgür, M. B. , Bilge, S. D. "Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 329-335
RIS TY - JOUR T1 - Seçilen keçi sürülerinde gB/gE ELISA kombinasyonu ve VNT kullanılarak BoHV-1 ve CpHV-1enfeksiyonlarının seroprevalansının araştırılması AU - Merve BaydinÖzgür, Seval DağalpBilge Y1 - 2017 PY - 2017 N1 - doi: 10.1501/Vetfak_0000002817 DO - 10.1501/Vetfak_0000002817 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 329 EP - 335 VL - 64 IS - 4 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002817 UR - https://doi.org/10.1501/Vetfak_0000002817 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks %A Merve Baydin Özgür , Seval Dağalp Bilge %T Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks %D 2017 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 64 %N 4 %R doi: 10.1501/Vetfak_0000002817 %U 10.1501/Vetfak_0000002817
ISNAD Özgür, Merve Baydin , Bilge, Seval Dağalp . "Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 / 4 (Aralık 2017): 329-335 . https://doi.org/10.1501/Vetfak_0000002817
AMA Özgür M. B. , Bilge S. D. Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks. Ankara Univ Vet Fak Derg. 2017; 64(4): 329-335.
Vancouver Özgür M. B. , Bilge S. D. Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64(4): 329-335.
IEEE M. B. Özgür ve S. D. Bilge , "Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 64, sayı. 4, ss. 329-335, Ara. 2017, doi:10.1501/Vetfak_0000002817