Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Broyler rasyonlarında probiyotik ve sinbiyotik kullanılmasının broyler performans ve bağırsakhistomorfolojisi üzerine etkisi

Yıl 2017, Cilt: 64 Sayı: 3, 183 - 189, 01.09.2017

Ali ÇALIK Burcu EKİM Alev Gürol BAYRAKTAROĞLU Ahmet ERGÜN Pınar SAÇAKLI

https://doi.org/10.1501/Vetfak_0000002797

Öz

Paenibacillus xylanexedens’in tek başına veya inulin veya laktuloz ile birlikte kullanılmasının erkek broylerlerin büyüme performansı ve bağırsak histomorfolojisi üzerine olan etkisi incelendi. Toplamda 168 adet günlük erkek civciv dört deneme grubuna ayrıldı: C, bazal rasyon (kontrol); P, 1×109 cfu/kg-yem P. xylanexedens; IN, 1×109 cfu/kg-yem P. xylanexedens ve %1 inulin; LAC, 1×109 cfu/kg-yem P. xylanexedens ve %0.5 laktuloz. P. xylanexedens ve sinbiyotik ilavesinin 1-21. günlerde broyler canlı ağırlık artışını (P<0.001) ve yemden yararlanma oranını (P=0.023) önemli düzeyde arttırdığı sonucuna varıldı. Tüm araştırma periyodu dikkate alındığında sinbiyotik ilavesinin canlı ağırlık artışını önemli ölçüde (P=0.013) etkilediği görüldü. Araştırmanın 42. gününde probiyotik ve sinbiyotik ilave edilen gruplarda ileum villus yüksekliğinin daha fazla olduğu tespit edildi (P=0.047). Sonuç olarak, P. xylanexedens’in broyler büyüme performansını ve bağırsak histomorfolojisini olumlu yönde etkilediği ve rasyonlarında probiyotik yem katkı maddesi olarak kullanılabileceği görüldü

Anahtar Kelimeler

Bağırsak histomorfolojisi, broyler, Paenibacillus xylanexedens, performans, probiyotik

Kaynakça

  • Ahmed ST, Islam M, Mun HS, et al. (2014): Effects of Bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broiler chickens. Poult Sci, 93, 1963-1971.
  • Al-Fataftah AR, Abdelqader A (2014): Effects of dietary Bacillus subtilis on heat-stressed broilers performance, intestinal morphology and microflora composition. Anim Feed Sci Tech, 198, 279-285.
  • Alzueta C, Rodriguez ML, Ortiz LT, et al. (2010): Effects of inulin on growth performance, nutrient digestibility and metabolisable energy in broiler chickens. Br Poult Sci, 51, 393-398.
  • Ash C, Priest FG, Collins MD (1993): Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. A van Leeuw, 64, 253-260.
  • Awad WA, Bohm J, Razzazi-Fazeli E, et al. (2006): Effect of addition of a probiotic microorganism to broiler diets contaminated with deoxynivalenol on performance and histological alterations of intestinal villi of broiler chickens. Poult Sci, 85, 974-979.
  • Awad WA, Ghareeb K, Abdel-Raheem S, et al. (2009): Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult Sci, 88, 49-56.
  • Awad WA, Ghareeb K, Bohm J (2010): Effect of addition of a probiotic micro-organism to broiler diet on intestinal mucosal architecture and electrophysiological parameters. J Anim Physiol Anim Nutr (Berl), 94, 486-494.
  • Bai SP, Wu AM, Ding XM, et al. (2013): Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poult Sci, 92, 663-670.
  • Barbosa TM, Serra CR, La Ragione RM, et al. (2005): Screening for bacillus isolates in the broiler gastrointestinal tract. Appl Environ Microbiol, 71, 968-978.
  • Blajman JE, Frizzo LS, Zbrun MV, et al. (2014): Probiotics and broiler growth performance: A meta- analysis of randomised controlled trials. Br Poult Sci, 55, 483-494.
  • Calik A, Ergun A (2015): Effect of lactulose supplementation on growth performance, intestinal histomorphology, cecal microbial population, and short- chain fatty acid composition of broiler chickens. Poult Sci, 94, 2173-2182.
  • Cartman ST, La Ragione RM, Woodward MJ (2008): Bacillus subtilis spores germinate in the chicken gastrointestinal tract. Appl Environ Microbiol,74, 5254- 5258.
  • Chavarri M, Maranon I, Ares R, et al. (2010): Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. Int J Food Microbiol, 142, 185- 189.
  • Cho JH, Kim IH (2014): Effects of lactulose supplementation on performance, blood profiles, excreta microbial shedding of Lactobacillus and Escherichia coli, relative organ weight and excreta noxious gas contents in broilers. J Anim Physiol Anim Nutr (Berl), 98, 424-430.
  • Culling CFA, Allison RT, Bar WT (1985): Cellular Pathology Technique, London: Butterworth and Co. Ltd.
  • Deniz G, Orman A, Cetinkaya F, et al. (2011): Effects of probiotic (Bacillus subtilis DSM 17299) supplementation on the caecal microflora and performance in broiler chickens. Rev Med Vet, 11, 538-545.
  • Farnworth ER (2001): Probiotics and prebiotics. In: Wildman REC, editor. Handbook of Nutraceuticals and Functional Foods. Boca Raton, USA: CRC Press; 2001. pp. 407-422.
  • Ghasemi HA, Kasani N, Taherpour K (2014): Effects of black cumin seed (Nigella sativa L.), a probiotic, a prebiotic and a synbiotic on growth performance, immune response and blood characteristics of male broilers. Livest Sci, 164, 128-134.
  • Gibson GR, Roberfroid MB (1995): Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J Nutr, 125, 1401-1412.
  • Hong HA, Duc le H, Cutting SM (2005): The use of bacterial spore formers as probiotics. FEMS Microbiol Rev, 29, 813-835.
  • Huff GR, Huff WE, Rath NC, et al. (2006): Limited treatment with beta-1,3/1,6-glucan improves production values of broiler chickens challenged with Escherichia coli. Poult Sci, 85, 613-618.
  • Jeong JS, Kim IH (2014): Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers. Poult Sci, 93, 3097-3103.
  • Kim GB, Seo YM, Kim CH, et al. (2011): Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poult Sci, 90, 75-82.
  • Kim KM, Kim MJ, Kim DH, et al. (2009): Characterization of Bacillus polyfermenticus KJS-2 as a probiotic. J Microbiol Biotechn, 19, 1013-1018.
  • Knarreborg A, Brockmann E, Hİybye K, et al. (2008): Bacillus subtilis (DSM17299) modulates the ileal microbial communities and improves growth performance in broilers. Int J Probiotics Prebiotics, 3, 83-86.
  • Lertcanawanichakul M, Sawangnop S (2011): A comparison of two methods used for measuring the antagonistic activity of Bacillus species. Walailak J Sci Tech, 5, 161-171.
  • Lutful Kabir SM (2009): The role of probiotics in the poultry industry. Int J Mol Sci, 10, 3531-3546.
  • Mookiah S, Sieo CC, Ramasamy K, et al. (2014): Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agric, 94, 341-348.
  • Mountzouris KC, Tsirtsikos P, Kalamara E, et al. (2007): Evaluation of the efficacy of a probiotic containing Lactobacillus, Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult Sci, 86, 309-317. Enterococcus, and
  • Mountzouris KC, Tsitrsikos P, Palamidi I, et al. (2010): Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poult Sci, 89, 58-67.
  • Nelson DM, Glawe AJ, Labeda DP, et al. (2009): Paenibacillus tundrae sp. nov. and Paenibacillus xylanexedens sp. nov., psychrotolerant, xylan-degrading bacteria from Alaskan tundra. Int J Syst Evol Micr, 59, 1708-1714. 32. NRC (1994): Nutrient Requirements of Poultry. Washington, DC: Natl. Acad. Press.
  • Rebole A, Ortiz LT, Rodriguez ML, et al. (2010): Effects of inulin and enzyme complex, individually or in combination, on growth performance, intestinal microflora, cecal histomorphology in broiler chickens fed a wheat- and barley-based diet. Poult Sci, 89, 276-286. and jejunal gut histomorphology and intestinal
  • Sen S, Ingale SL, Kim YW, et al. (2012): Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res Vet Sci, 93, 264-268.
  • Song J, Xiao K, Ke YL, et al. (2014): Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poult Sci, 93, 581-588.
  • Yason CV, Summers BA, Schat KA (1987): Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: Pathology. Am J Vet Res, 48, 927-938.

Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology

Yıl 2017, Cilt: 64 Sayı: 3, 183 - 189, 01.09.2017

Ali ÇALIK Burcu EKİM Alev Gürol BAYRAKTAROĞLU Ahmet ERGÜN Pınar SAÇAKLI

https://doi.org/10.1501/Vetfak_0000002797

Öz

The effects of Paenibacillus xylanexedens, alone or in combination with inulin and lactulose were evaluated on growth performance and intestinal histomorphology of male broilers. A total of 168 one-day-old male broiler chickens were randomly allocated to 4 experimental groups as follows: C, basal diet (control; without supplementation); P, 1×109 cfu/kg-feed P. xylanexedens; IN, 1×109 cfu/kg-feed P. xylanexedens and 1% inulin; LAC, 1×109 cfu/kg-feed P. xylanexedens and 0.5% lactulose. Addition of P. xylanexedens and synbiotics significantly increased broiler body weight gain (P<0.001) and feed conversion ratio (P=0.023) from 1 to 21 day of age. Moreover, a significant increase in body weight gain was observed when birds were fed with synbiotic supplemented diets during the overall experimental period (P=0.013). The addition of probiotic and synbiotics increased (P=0.047) villus height in ileum on day 42. Our results indicate that P. xylanexedens can be used as a probiotic feed additive in broiler diets and can improve broiler performance and intestinal histomorphology

Anahtar Kelimeler

Broiler, intestinal histomorphology, Paenibacillus xylanexedens, performance, probiotic

Kaynakça

  • Ahmed ST, Islam M, Mun HS, et al. (2014): Effects of Bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broiler chickens. Poult Sci, 93, 1963-1971.
  • Al-Fataftah AR, Abdelqader A (2014): Effects of dietary Bacillus subtilis on heat-stressed broilers performance, intestinal morphology and microflora composition. Anim Feed Sci Tech, 198, 279-285.
  • Alzueta C, Rodriguez ML, Ortiz LT, et al. (2010): Effects of inulin on growth performance, nutrient digestibility and metabolisable energy in broiler chickens. Br Poult Sci, 51, 393-398.
  • Ash C, Priest FG, Collins MD (1993): Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. A van Leeuw, 64, 253-260.
  • Awad WA, Bohm J, Razzazi-Fazeli E, et al. (2006): Effect of addition of a probiotic microorganism to broiler diets contaminated with deoxynivalenol on performance and histological alterations of intestinal villi of broiler chickens. Poult Sci, 85, 974-979.
  • Awad WA, Ghareeb K, Abdel-Raheem S, et al. (2009): Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult Sci, 88, 49-56.
  • Awad WA, Ghareeb K, Bohm J (2010): Effect of addition of a probiotic micro-organism to broiler diet on intestinal mucosal architecture and electrophysiological parameters. J Anim Physiol Anim Nutr (Berl), 94, 486-494.
  • Bai SP, Wu AM, Ding XM, et al. (2013): Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poult Sci, 92, 663-670.
  • Barbosa TM, Serra CR, La Ragione RM, et al. (2005): Screening for bacillus isolates in the broiler gastrointestinal tract. Appl Environ Microbiol, 71, 968-978.
  • Blajman JE, Frizzo LS, Zbrun MV, et al. (2014): Probiotics and broiler growth performance: A meta- analysis of randomised controlled trials. Br Poult Sci, 55, 483-494.
  • Calik A, Ergun A (2015): Effect of lactulose supplementation on growth performance, intestinal histomorphology, cecal microbial population, and short- chain fatty acid composition of broiler chickens. Poult Sci, 94, 2173-2182.
  • Cartman ST, La Ragione RM, Woodward MJ (2008): Bacillus subtilis spores germinate in the chicken gastrointestinal tract. Appl Environ Microbiol,74, 5254- 5258.
  • Chavarri M, Maranon I, Ares R, et al. (2010): Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. Int J Food Microbiol, 142, 185- 189.
  • Cho JH, Kim IH (2014): Effects of lactulose supplementation on performance, blood profiles, excreta microbial shedding of Lactobacillus and Escherichia coli, relative organ weight and excreta noxious gas contents in broilers. J Anim Physiol Anim Nutr (Berl), 98, 424-430.
  • Culling CFA, Allison RT, Bar WT (1985): Cellular Pathology Technique, London: Butterworth and Co. Ltd.
  • Deniz G, Orman A, Cetinkaya F, et al. (2011): Effects of probiotic (Bacillus subtilis DSM 17299) supplementation on the caecal microflora and performance in broiler chickens. Rev Med Vet, 11, 538-545.
  • Farnworth ER (2001): Probiotics and prebiotics. In: Wildman REC, editor. Handbook of Nutraceuticals and Functional Foods. Boca Raton, USA: CRC Press; 2001. pp. 407-422.
  • Ghasemi HA, Kasani N, Taherpour K (2014): Effects of black cumin seed (Nigella sativa L.), a probiotic, a prebiotic and a synbiotic on growth performance, immune response and blood characteristics of male broilers. Livest Sci, 164, 128-134.
  • Gibson GR, Roberfroid MB (1995): Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J Nutr, 125, 1401-1412.
  • Hong HA, Duc le H, Cutting SM (2005): The use of bacterial spore formers as probiotics. FEMS Microbiol Rev, 29, 813-835.
  • Huff GR, Huff WE, Rath NC, et al. (2006): Limited treatment with beta-1,3/1,6-glucan improves production values of broiler chickens challenged with Escherichia coli. Poult Sci, 85, 613-618.
  • Jeong JS, Kim IH (2014): Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers. Poult Sci, 93, 3097-3103.
  • Kim GB, Seo YM, Kim CH, et al. (2011): Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poult Sci, 90, 75-82.
  • Kim KM, Kim MJ, Kim DH, et al. (2009): Characterization of Bacillus polyfermenticus KJS-2 as a probiotic. J Microbiol Biotechn, 19, 1013-1018.
  • Knarreborg A, Brockmann E, Hİybye K, et al. (2008): Bacillus subtilis (DSM17299) modulates the ileal microbial communities and improves growth performance in broilers. Int J Probiotics Prebiotics, 3, 83-86.
  • Lertcanawanichakul M, Sawangnop S (2011): A comparison of two methods used for measuring the antagonistic activity of Bacillus species. Walailak J Sci Tech, 5, 161-171.
  • Lutful Kabir SM (2009): The role of probiotics in the poultry industry. Int J Mol Sci, 10, 3531-3546.
  • Mookiah S, Sieo CC, Ramasamy K, et al. (2014): Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agric, 94, 341-348.
  • Mountzouris KC, Tsirtsikos P, Kalamara E, et al. (2007): Evaluation of the efficacy of a probiotic containing Lactobacillus, Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult Sci, 86, 309-317. Enterococcus, and
  • Mountzouris KC, Tsitrsikos P, Palamidi I, et al. (2010): Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poult Sci, 89, 58-67.
  • Nelson DM, Glawe AJ, Labeda DP, et al. (2009): Paenibacillus tundrae sp. nov. and Paenibacillus xylanexedens sp. nov., psychrotolerant, xylan-degrading bacteria from Alaskan tundra. Int J Syst Evol Micr, 59, 1708-1714. 32. NRC (1994): Nutrient Requirements of Poultry. Washington, DC: Natl. Acad. Press.
  • Rebole A, Ortiz LT, Rodriguez ML, et al. (2010): Effects of inulin and enzyme complex, individually or in combination, on growth performance, intestinal microflora, cecal histomorphology in broiler chickens fed a wheat- and barley-based diet. Poult Sci, 89, 276-286. and jejunal gut histomorphology and intestinal
  • Sen S, Ingale SL, Kim YW, et al. (2012): Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res Vet Sci, 93, 264-268.
  • Song J, Xiao K, Ke YL, et al. (2014): Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poult Sci, 93, 581-588.
  • Yason CV, Summers BA, Schat KA (1987): Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: Pathology. Am J Vet Res, 48, 927-938.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA49KT34MG
Bölüm Araştırma Makalesi
Yazarlar

Ali ÇALIK

Burcu EKİM

Alev Gürol BAYRAKTAROĞLU

Ahmet ERGÜN

Pınar SAÇAKLI

Yayımlanma Tarihi 1 Eylül 2017
Yayınlandığı Sayı Yıl 2017Cilt: 64 Sayı: 3

Kaynak Göster

Bibtex @editöre mektup { auvfd526061, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2017}, volume = {64}, number = {3}, pages = {183 - 189}, doi = {10.1501/Vetfak\_0000002797}, title = {Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology}, key = {cite}, author = {Çalık, Ali and Ekim, Burcu and Bayraktaroğlu, Alev Gürol and Ergün, Ahmet and Saçaklı, Pınar} }
APA Çalık, A. , Ekim, B. , Bayraktaroğlu, A. G. , Ergün, A. & Saçaklı, P. (2017). Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 64 (3) , 183-189 . DOI: 10.1501/Vetfak_0000002797
MLA Çalık, A. , Ekim, B. , Bayraktaroğlu, A. G. , Ergün, A. , Saçaklı, P. "Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 183-189 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43287/526061>
Chicago Çalık, A. , Ekim, B. , Bayraktaroğlu, A. G. , Ergün, A. , Saçaklı, P. "Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 183-189
RIS TY - JOUR T1 - Broyler rasyonlarında probiyotik ve sinbiyotik kullanılmasının broyler performans ve bağırsakhistomorfolojisi üzerine etkisi AU - AliÇalık, BurcuEkim, Alev GürolBayraktaroğlu, AhmetErgün, PınarSaçaklı Y1 - 2017 PY - 2017 N1 - doi: 10.1501/Vetfak_0000002797 DO - 10.1501/Vetfak_0000002797 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 183 EP - 189 VL - 64 IS - 3 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002797 UR - https://doi.org/10.1501/Vetfak_0000002797 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology %A Ali Çalık , Burcu Ekim , Alev Gürol Bayraktaroğlu , Ahmet Ergün , Pınar Saçaklı %T Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology %D 2017 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 64 %N 3 %R doi: 10.1501/Vetfak_0000002797 %U 10.1501/Vetfak_0000002797
ISNAD Çalık, Ali , Ekim, Burcu , Bayraktaroğlu, Alev Gürol , Ergün, Ahmet , Saçaklı, Pınar . "Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 / 3 (Eylül 2017): 183-189 . https://doi.org/10.1501/Vetfak_0000002797
AMA Çalık A. , Ekim B. , Bayraktaroğlu A. G. , Ergün A. , Saçaklı P. Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology. Ankara Univ Vet Fak Derg. 2017; 64(3): 183-189.
Vancouver Çalık A. , Ekim B. , Bayraktaroğlu A. G. , Ergün A. , Saçaklı P. Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64(3): 183-189.
IEEE A. Çalık , B. Ekim , A. G. Bayraktaroğlu , A. Ergün ve P. Saçaklı , "Effects of dietary probiotic and synbiotic supplementation on broiler growth performance and intestinal histomorphology", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 64, sayı. 3, ss. 183-189, Eyl. 2017, doi:10.1501/Vetfak_0000002797