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Investigation of Neospora caninum tissue cysts in cattle

Yıl 2017, Cilt: 64 Sayı: 1, 45 - 49, 01.03.2017

Kader Yıldız Sami Gökpınar

https://doi.org/10.1501/Vetfak_0000002772
Cited By: 1

Öz

In this study, it was aimed to detect N. caninum seroprevalence in 200 cattle randomly selected and to detect tissue cysts of N. caninum in tissue samples of brain and skeletal muscles of the seropositive cattle. Seropositivity of N. caninum was detected in 5.5% of cattle (11/200). N. caninum tissue cysts were not observed in tissue samples of cattle examined with percoll gradient centrifugation. However, N. caninum DNA was amplified in brain tissues of two seropositive bulls (18.1%).Özet: Bu çalışmada rastgele seçilen 200 sığırda N. caninum seropozitivitesinin belirlenmesi, seropozitif sığırların beyin ve
iskelet kası doku örneklerinde N. caninum doku kistlerinin percoll gradient santrifüj ve PCR yöntemleri ile araştırılması amaçlanmıştır.
Çalışma sonucunda sığırlarda %5,5 oranında N. caninum seropozitivitesi belirlenmiştir (11/200). Percoll gradient santrifüj yöntemi ile
incelenen seropozitif sığırlara ait doku örneklerinde parazite ait doku kistleri tespit edilmemiştir. Ancak iki seropozitif boğanın beyin
dokusunda N. caninum’a ait DNA amplifiye edilmiştir (%18.1). 

Anahtar Kelimeler

Cattle Neospora caninum serology tissue cyst

Kaynakça

  • Akça A, Gokce HI, Guy CS ve ark. (2005): Prevalence of antibodies to Neospora caninum in local and imported cattle breeds in the Kars province of Turkey. Res Vet Sci, 78, 123-126.
  • Aktaş M, Şaki CE, Altay K ve ark. (2005): Doğu Anadolu Bölgesinin bazı illerinde bulunan sığırlarda Neospora caninum’un araştırılması. Türkiye Parazitol Derg, 29, 22- 25.
  • Baillargeon P, Fecteau G, Pare J ve ark. (2001): Evaluation of the embryo transfer procedure proposed by the International Embryo Transfer Society as a method of controlling vertical transmission of Neospora caninum in cattle. J Am Vet Med Assoc, 218, 1803-1806.
  • Batmaz H, Şentürk S, Aydın L (2004): Clinical neosporosis in a dog in Turkey. Aust Vet Pract, 34, 108-110.
  • Cavalcante GT, Monteiro RM, Soares RM ve ark. (2011): Shedding of Neospora caninum oocysts by dogs fed different tissues from naturally infected cattle. Vet Parasitol, 179, 220-223.
  • Çelik HA, Kozan E, Eser M ve ark. (2013): A research on seroprevalence of Neospora caninum in cattle. Vet J Ankara Univ, 60, 99-102.
  • Coşkun SZ, Aydin L, Bauer C (2000): Seroprevalence of Neospora caninum infection in domestic dogs in Turkey. Vet Rec, 146, 649.
  • Dubey JP, Hattel AL, Lindsay DS ve ark. (1988): Neonatal Neospora caninum infection in dogs: Isolation of the causative agent and experimental transmission. J Am Vet Med Assoc, 193, 1259-1263.
  • Dubey JP, Bjerkas I, Bjorkman C ve ark. (2002): Redescription of Neospora caninum and its differentiation from related coccidia. Int J Parasitol, 32, 929-946.
  • Dubey JP, Jenkins MC, Rajendran C ve ark. (2011): Gray wolf (Canis lupus) is a natural deŞnitive host for Neospora caninum. Vet Parasitol, 181, 382-387.
  • Dubey JP, Schares G (2011): Neosporosis in animals-the last five years. Vet Parasitol, 180, 90-108.
  • Dubey JP, Sreekumar C, Knickman E ve ark. (2004): Biologic, morphologic, and molecular characterisation of Neospora caninum isolates from littermate dogs. Int J Parasitol, 34, 1157-1167.
  • Düzlü Ö, İnci A, Yıldırım A ve ark. (2014): Köpek periferal kan örneklerinde Neosopora caninum ve Toxoplasma gondii takizoitlerinin Taqman prob bazlı Real time PCR ile araştırılması. Ankara Üniv Vet Fak Derg, 61, 283-288.
  • Eggleston TL, Fitzpatrick E, Hager KM (2008): Parasitology as a teaching tool: Isolation of Apicomplexan cysts from store-bought meat. CBE Life Sci Educ, 7, 184- 192.
  • Fioretti DP, Pasquai P, Diaferia M ve ark. (2003): Neospora caninum infection and congenital transmission: Serological and parasitological study of cows up to the fourth gestation. J Vet Med B, 50, 399-404.
  • Gondim LF, Gao L, McAllister MM (2002): Improved production of Neospora caninum oocysts, cyclical oral transmission between dogs and cattle, and in vitro isolation from oocysts. J Parasitol, 88, 1159-1163.
  • Gondim LFP, McAllister MM, Pitt WC ve ark. (2004): Coyotes (Canis latris) are definitive hosts of Neospora caninum. Int J Parasitol, 34,159-161.
  • Gondim LFP, McAllister MM, Mateus-Pinilla NE ve ark. (2005): Transmission of Neospora caninum between wild and domestic animals. J Parasitol, 90, 1361-1365.
  • Goodswen SJ, Kennedy PJ, Ellis JT (2013): A review of the infection, genetics, and evolution of Neospora caninum: From the past to the present. Infect Genet Evol, 13, 133- 150.
  • İça A, Yıldırım A, Düzlü O ve ark. (2006): Kayseri yöresinde sığırlarda Neospora caninum’un seroprevalansı. Türkiye Parazitol Derg, 30, 92-94.
  • Koyama T, Kobayashi Y, Omata Y ve ark. (2001): Isolation of Neospora caninum from the brain of a pregnant sheep. J Parasitol, 87, 1486-1488.
  • Kul O, Kabakci N, Yildiz K ve ark. (2009): Neospora caninum associated with epidemic abortions in dairy cattle: The first clinical neosporosis report in Turkey. Vet Parasitol, 159, 69-72.
  • McAllister MM, Dubey JP, Lindsay DS ve ark. (1998): Dogs are definitive hosts of Neospora caninum. Int J Parasitol, 28, 1473-1478.
  • McGarry JW, Stockton CM, Williams DJL ve ark. (2003): Protracted shedding of oocysts of Neospora caninum by a naturally infected foxhound. J Parasitol, 89, 628-630.
  • Mor N, Akça A (2012): Kars yöresinde sığır ve köpeklerde Neospora caninum üzerine epidemiyolojik araştırmalar: Gruplararası çalışma. Kafkas Üniv Vet Fak Derg, 18, 193- 199.
  • More G, Basso W, Bacigalupe D ve ark. (2008): Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle. Parasitol Res, 102, 671-675.
  • Nishimura M, Kohara J, Hiasa J ve ark. (2013): Tissue distribution of Neospora caninum in experimentally infected cattle. Clin Vaccin Immunol, 20, 309-312.
  • Ocal N, Atmaca HT, Albay MK ve ark. (2014): Neospora caninum infection status of cattle and dogs in modern and rural dairy farms in Turkey. Turk J Vet Anim Sci, 38, 161- 168.
  • Okeoma CM, Williamson NB, Pomroy WE ve ark. (2004): Isolation and molecular characterisation of Neospora caninum in cattle in New Zealand. NZVJ, 52, 364-370.
  • Ortega-Mora LM, Gottstein B, Conraths FJ ve ark. (2007): Protozoal Abortion in Farm Ruminants: Guidelines for Diagnosis and Control. CAB International, Wallingford.
  • Öncel T, Bıyıkoğlu G (2003): Sakarya yöresi süt sığırlarında Neosporosis caninum. Uludağ Üniv Vet Fak Derg, 22, 87-89.
  • Pare J, Thurmond MC, Hietala SK (1996): Congenital Neospora caninum infection in dairy cattle and associated calfhood mortality. Can J Vet Res, 60, 133-139.
  • Peters M, Lutkefels E, Heckeroth AR ve ark. (2001): Immunohistochemical and ultrastructural evidence for Neospora caninum tissue cysts in skeletal muscles of naturally infected dogs and cattle. Int J Parasitol, 31, 1144- 1148.
  • Pişkin FÇ, Ütük AE (2009): Prevalence of Neospora caninum in cows with stillbirth and abortion. Etlik Vet Mikrobiyol Derg, 20, 23-26.
  • Rodrigues AAR, Gennari SM, Aguiar DM ve ark. (2004): Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil. Vet Parasitol, 124, 139-150.
  • Santos S, de Souza Costa K, Gondim LQ ve ark. (2010): Investigation of Neospora caninum. Hammondia sp. and Toxoplasma gondii in tissues from slaughtered beef cattle in Bahia, Brazil. Parasitol Res, 106, 457-461.
  • Sawada M, Kondo H, Tomioka Y ve ark. (2000): Isolation of Neospora caninum from the brain of a naturally infected adult dairy cow. Vet Parasitol, 90, 247-252.
  • Sevgili M, Altas MG, Keskin O (2005): Seroprevalence of Neospora caninum in cattle in the province of Şanlıurfa. Turk J Vet Anim Sci, 29, 127-130.
  • Şimşek S, Ütük AE, Köroğlu E ve ark. (2008): Seroprevalence of Neospora caninum in repeat breeder dairy cows in Turkey. Archiv Tierzucht, 51, 143-148.
  • The Taxonomicon. [http://taxonomicon.taxonomy.nl/TaxonTree.aspx?id= 637468&src=0], (17.10.2015)
  • Trees AJ, Williams DJL (2005): Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends Parasitol, 21, 558-561.
  • Trees AJ, Davison HC, Innes EA ve ark. (1999): Towards evaluating the economic impact of bovine neosporosis. Int J Parasitol, 29, 1195-1200. W, 43. Wouda Bartels CJM, Characteristics of Neospora caninum-associated abortion storms in dairy herds in the Netherlands (1995-1997). Theriogenology, 52, 233-245. Moen AR (1999):
  • Yildiz K, Kul O, Babur C ve ark. (2009): Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: special emphasis to serologic coexistence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes. Vet Parasitol, 164, 306-310.
  • Yildiz K, Kul O, Gokpınar S ve ark. (2014): The relationship between seropositivity and occurence of tissue cysts in sheep naturally infected with Toxoplasma gondii. Turk J Vet Anim Sci, 38, 169-175
  • Yildiz K, Yasa Duru S, Yağcı BB ve ark. (2009): Seroprevalence of antibodies to Neospora caninum and co- existence with Toxoplasma gondii in dogs. Türkiye Parazitol Derg, 33, 116-119.

Sığırlarda Neospora caninum doku kistlerinin araştırılması

Yıl 2017, Cilt: 64 Sayı: 1, 45 - 49, 01.03.2017

Kader Yıldız Sami Gökpınar

https://doi.org/10.1501/Vetfak_0000002772
Cited By: 1

Öz

Bu çalışmada rastgele seçilen 200 sığırda N. caninum seropozitivitesinin belirlenmesi, seropozitif sığırların beyin ve iskelet kası doku örneklerinde N. caninum doku kistlerinin percoll gradient santrifüj ve PCR yöntemleri ile araştırılması amaçlanmıştır. Çalışma sonucunda sığırlarda %5,5 oranında N. caninum seropozitivitesi belirlenmiştir (11/200). Percoll gradient santrifüj yöntemi ile incelenen seropozitif sığırlara ait doku örneklerinde parazite ait doku kistleri tespit edilmemiştir. Ancak iki seropozitif boğanın beyin dokusunda N. caninum’a ait DNA amplifiye edilmiştir (%18.1)

Anahtar Kelimeler

Doku kisti Neospora caninum seroloji sığır

Kaynakça

  • Akça A, Gokce HI, Guy CS ve ark. (2005): Prevalence of antibodies to Neospora caninum in local and imported cattle breeds in the Kars province of Turkey. Res Vet Sci, 78, 123-126.
  • Aktaş M, Şaki CE, Altay K ve ark. (2005): Doğu Anadolu Bölgesinin bazı illerinde bulunan sığırlarda Neospora caninum’un araştırılması. Türkiye Parazitol Derg, 29, 22- 25.
  • Baillargeon P, Fecteau G, Pare J ve ark. (2001): Evaluation of the embryo transfer procedure proposed by the International Embryo Transfer Society as a method of controlling vertical transmission of Neospora caninum in cattle. J Am Vet Med Assoc, 218, 1803-1806.
  • Batmaz H, Şentürk S, Aydın L (2004): Clinical neosporosis in a dog in Turkey. Aust Vet Pract, 34, 108-110.
  • Cavalcante GT, Monteiro RM, Soares RM ve ark. (2011): Shedding of Neospora caninum oocysts by dogs fed different tissues from naturally infected cattle. Vet Parasitol, 179, 220-223.
  • Çelik HA, Kozan E, Eser M ve ark. (2013): A research on seroprevalence of Neospora caninum in cattle. Vet J Ankara Univ, 60, 99-102.
  • Coşkun SZ, Aydin L, Bauer C (2000): Seroprevalence of Neospora caninum infection in domestic dogs in Turkey. Vet Rec, 146, 649.
  • Dubey JP, Hattel AL, Lindsay DS ve ark. (1988): Neonatal Neospora caninum infection in dogs: Isolation of the causative agent and experimental transmission. J Am Vet Med Assoc, 193, 1259-1263.
  • Dubey JP, Bjerkas I, Bjorkman C ve ark. (2002): Redescription of Neospora caninum and its differentiation from related coccidia. Int J Parasitol, 32, 929-946.
  • Dubey JP, Jenkins MC, Rajendran C ve ark. (2011): Gray wolf (Canis lupus) is a natural deŞnitive host for Neospora caninum. Vet Parasitol, 181, 382-387.
  • Dubey JP, Schares G (2011): Neosporosis in animals-the last five years. Vet Parasitol, 180, 90-108.
  • Dubey JP, Sreekumar C, Knickman E ve ark. (2004): Biologic, morphologic, and molecular characterisation of Neospora caninum isolates from littermate dogs. Int J Parasitol, 34, 1157-1167.
  • Düzlü Ö, İnci A, Yıldırım A ve ark. (2014): Köpek periferal kan örneklerinde Neosopora caninum ve Toxoplasma gondii takizoitlerinin Taqman prob bazlı Real time PCR ile araştırılması. Ankara Üniv Vet Fak Derg, 61, 283-288.
  • Eggleston TL, Fitzpatrick E, Hager KM (2008): Parasitology as a teaching tool: Isolation of Apicomplexan cysts from store-bought meat. CBE Life Sci Educ, 7, 184- 192.
  • Fioretti DP, Pasquai P, Diaferia M ve ark. (2003): Neospora caninum infection and congenital transmission: Serological and parasitological study of cows up to the fourth gestation. J Vet Med B, 50, 399-404.
  • Gondim LF, Gao L, McAllister MM (2002): Improved production of Neospora caninum oocysts, cyclical oral transmission between dogs and cattle, and in vitro isolation from oocysts. J Parasitol, 88, 1159-1163.
  • Gondim LFP, McAllister MM, Pitt WC ve ark. (2004): Coyotes (Canis latris) are definitive hosts of Neospora caninum. Int J Parasitol, 34,159-161.
  • Gondim LFP, McAllister MM, Mateus-Pinilla NE ve ark. (2005): Transmission of Neospora caninum between wild and domestic animals. J Parasitol, 90, 1361-1365.
  • Goodswen SJ, Kennedy PJ, Ellis JT (2013): A review of the infection, genetics, and evolution of Neospora caninum: From the past to the present. Infect Genet Evol, 13, 133- 150.
  • İça A, Yıldırım A, Düzlü O ve ark. (2006): Kayseri yöresinde sığırlarda Neospora caninum’un seroprevalansı. Türkiye Parazitol Derg, 30, 92-94.
  • Koyama T, Kobayashi Y, Omata Y ve ark. (2001): Isolation of Neospora caninum from the brain of a pregnant sheep. J Parasitol, 87, 1486-1488.
  • Kul O, Kabakci N, Yildiz K ve ark. (2009): Neospora caninum associated with epidemic abortions in dairy cattle: The first clinical neosporosis report in Turkey. Vet Parasitol, 159, 69-72.
  • McAllister MM, Dubey JP, Lindsay DS ve ark. (1998): Dogs are definitive hosts of Neospora caninum. Int J Parasitol, 28, 1473-1478.
  • McGarry JW, Stockton CM, Williams DJL ve ark. (2003): Protracted shedding of oocysts of Neospora caninum by a naturally infected foxhound. J Parasitol, 89, 628-630.
  • Mor N, Akça A (2012): Kars yöresinde sığır ve köpeklerde Neospora caninum üzerine epidemiyolojik araştırmalar: Gruplararası çalışma. Kafkas Üniv Vet Fak Derg, 18, 193- 199.
  • More G, Basso W, Bacigalupe D ve ark. (2008): Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle. Parasitol Res, 102, 671-675.
  • Nishimura M, Kohara J, Hiasa J ve ark. (2013): Tissue distribution of Neospora caninum in experimentally infected cattle. Clin Vaccin Immunol, 20, 309-312.
  • Ocal N, Atmaca HT, Albay MK ve ark. (2014): Neospora caninum infection status of cattle and dogs in modern and rural dairy farms in Turkey. Turk J Vet Anim Sci, 38, 161- 168.
  • Okeoma CM, Williamson NB, Pomroy WE ve ark. (2004): Isolation and molecular characterisation of Neospora caninum in cattle in New Zealand. NZVJ, 52, 364-370.
  • Ortega-Mora LM, Gottstein B, Conraths FJ ve ark. (2007): Protozoal Abortion in Farm Ruminants: Guidelines for Diagnosis and Control. CAB International, Wallingford.
  • Öncel T, Bıyıkoğlu G (2003): Sakarya yöresi süt sığırlarında Neosporosis caninum. Uludağ Üniv Vet Fak Derg, 22, 87-89.
  • Pare J, Thurmond MC, Hietala SK (1996): Congenital Neospora caninum infection in dairy cattle and associated calfhood mortality. Can J Vet Res, 60, 133-139.
  • Peters M, Lutkefels E, Heckeroth AR ve ark. (2001): Immunohistochemical and ultrastructural evidence for Neospora caninum tissue cysts in skeletal muscles of naturally infected dogs and cattle. Int J Parasitol, 31, 1144- 1148.
  • Pişkin FÇ, Ütük AE (2009): Prevalence of Neospora caninum in cows with stillbirth and abortion. Etlik Vet Mikrobiyol Derg, 20, 23-26.
  • Rodrigues AAR, Gennari SM, Aguiar DM ve ark. (2004): Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil. Vet Parasitol, 124, 139-150.
  • Santos S, de Souza Costa K, Gondim LQ ve ark. (2010): Investigation of Neospora caninum. Hammondia sp. and Toxoplasma gondii in tissues from slaughtered beef cattle in Bahia, Brazil. Parasitol Res, 106, 457-461.
  • Sawada M, Kondo H, Tomioka Y ve ark. (2000): Isolation of Neospora caninum from the brain of a naturally infected adult dairy cow. Vet Parasitol, 90, 247-252.
  • Sevgili M, Altas MG, Keskin O (2005): Seroprevalence of Neospora caninum in cattle in the province of Şanlıurfa. Turk J Vet Anim Sci, 29, 127-130.
  • Şimşek S, Ütük AE, Köroğlu E ve ark. (2008): Seroprevalence of Neospora caninum in repeat breeder dairy cows in Turkey. Archiv Tierzucht, 51, 143-148.
  • The Taxonomicon. [http://taxonomicon.taxonomy.nl/TaxonTree.aspx?id= 637468&src=0], (17.10.2015)
  • Trees AJ, Williams DJL (2005): Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends Parasitol, 21, 558-561.
  • Trees AJ, Davison HC, Innes EA ve ark. (1999): Towards evaluating the economic impact of bovine neosporosis. Int J Parasitol, 29, 1195-1200. W, 43. Wouda Bartels CJM, Characteristics of Neospora caninum-associated abortion storms in dairy herds in the Netherlands (1995-1997). Theriogenology, 52, 233-245. Moen AR (1999):
  • Yildiz K, Kul O, Babur C ve ark. (2009): Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: special emphasis to serologic coexistence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes. Vet Parasitol, 164, 306-310.
  • Yildiz K, Kul O, Gokpınar S ve ark. (2014): The relationship between seropositivity and occurence of tissue cysts in sheep naturally infected with Toxoplasma gondii. Turk J Vet Anim Sci, 38, 169-175
  • Yildiz K, Yasa Duru S, Yağcı BB ve ark. (2009): Seroprevalence of antibodies to Neospora caninum and co- existence with Toxoplasma gondii in dogs. Türkiye Parazitol Derg, 33, 116-119.
Toplam 45 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA94MF78YB
Bölüm Araştırma Makalesi
Yazarlar

Kader Yıldız

Sami Gökpınar

Yayımlanma Tarihi 1 Mart 2017
Yayımlandığı Sayı Yıl 2017Cilt: 64 Sayı: 1

Kaynak Göster

APA Yıldız, K., & Gökpınar, S. (2017). Investigation of Neospora caninum tissue cysts in cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(1), 45-49. https://doi.org/10.1501/Vetfak_0000002772
AMA Yıldız K, Gökpınar S. Investigation of Neospora caninum tissue cysts in cattle. Ankara Univ Vet Fak Derg. Mart 2017;64(1):45-49. doi:10.1501/Vetfak_0000002772
Chicago Yıldız, Kader, ve Sami Gökpınar. “Investigation of Neospora Caninum Tissue Cysts in Cattle”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64, sy. 1 (Mart 2017): 45-49. https://doi.org/10.1501/Vetfak_0000002772.
EndNote Yıldız K, Gökpınar S (01 Mart 2017) Investigation of Neospora caninum tissue cysts in cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 1 45–49.
IEEE K. Yıldız ve S. Gökpınar, “Investigation of Neospora caninum tissue cysts in cattle”, Ankara Univ Vet Fak Derg, c. 64, sy. 1, ss. 45–49, 2017, doi: 10.1501/Vetfak_0000002772.
ISNAD Yıldız, Kader - Gökpınar, Sami. “Investigation of Neospora Caninum Tissue Cysts in Cattle”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 64/1 (Mart 2017), 45-49. https://doi.org/10.1501/Vetfak_0000002772.
JAMA Yıldız K, Gökpınar S. Investigation of Neospora caninum tissue cysts in cattle. Ankara Univ Vet Fak Derg. 2017;64:45–49.
MLA Yıldız, Kader ve Sami Gökpınar. “Investigation of Neospora Caninum Tissue Cysts in Cattle”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 64, sy. 1, 2017, ss. 45-49, doi:10.1501/Vetfak_0000002772.
Vancouver Yıldız K, Gökpınar S. Investigation of Neospora caninum tissue cysts in cattle. Ankara Univ Vet Fak Derg. 2017;64(1):45-9.

Cited By

The incidence of Neospora caninum in dairy cows with abortion and infertility problems
Kocatepe Veterinary Journal
https://doi.org/10.30607/kvj.1009265