Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Genetic diversity between rahvan (pacing) horses in Turkey

Yıl 2016, Cilt: 63 Sayı: 2, 201 - 210, 01.06.2016

Banu YÜCEER Metin ERDOĞAN Cevdet YARALI Bora ÖZARSLAN Ceyhan ÖZBEYAZ

https://doi.org/10.1501/Vetfak_0000002730

Öz

This study was conducted to determine the genetic structures of Rahvan (pacing) horses from 7 different geographical region in Turkey. For this purpose, 17 microsatellite loci were used. Blood or hair root samples were collected from 353 Rahvan horses aging 4 years or older. Also, genetic similarities or differences were investigated between Rahvan, Arabian (n=53) and Thoroughbred (n=51) horses. As a result of genetic analysis, FIS,FIT and FST values of Rahvan horses were calculated as 0.0982, 0.0999 and 0.0019, respectively. According to region (The Central Anatolia, Black Sea, Aegean, Marmara, Eastern Anatolia, Southeastern Anatolia and Mediterranean Region), observed heterozigosities (Ho) were estimated as 0.718, 0.688, 0.711, 0.719, 0.722, 0.700 and 0.708, respectively. Numbers of alleles of Rahvan, Arabian and Thoroughbred horses were 10.65, 5.18, and 5.00, respectively. Factorial Correspondences Analysis was performed to reveal differences among populations. Rahvan, Arabian and Thoroughbred horses were all clustered as separate groups. Also, structure analysis were given the same results. The results showed that Rahvan horses were similar in all regions in terms of microsatellite loci, but quite different from Arabian and Thoroughbred horses

Anahtar Kelimeler

Genetic diversity, horse, microsatellite, rahvan, Turkey

Kaynakça

  • 1. Aberle KS, Hamann H, Drögemüller C, Distl O (2004): Genetic diversity in German draught horse breeds compared with a group of primitive, riding and wild horses by means of microsatellite DNA markers. Anim Genet, 35, 270-277.
  • 2. Achmann R, Curik I, Dovc P, Kavar T, Bodo I, Habe F, Marti E, Sölkner J, Brem G (2004): Microsatellite diversity, population subdivision and gene flow in the Lipizzan horse. Anim Genet, 35, 285-292.
  • 3. Anonim (2014b): QIAamp DNA Mini Kit. Erişim Adresi: www.qiagen.com/products/catalog/sampletechnologies/dna-sample-technologies/genomic-dna/ qiaamp-dna-mini-kit Erişim Tarihi: 07.08.2014
  • 4. Apostolidis AP, Mamuris Z, Karkavelia E, Alifakiotis T (2001): Comparison of Greek breeds of horses using RAPD markers. J Anim Breed Genet, 118, 47-56.
  • 5. Barcaccia G, Felicetti M, Galla G, Capomaccio S, Cappelli K, Albertini E, Buttazzoni L, Pieramati C, Silvestrelli M, Supplizi AV (2013): Molecular analysis of genetic diversity, population structure and inbreeding level of the Italian Lipizzan horse. Livest Sci, 151, 124-133.
  • 6. Batu S (1962): Türk Atları ve At Yetiştirme Bilgisi, Ankara.
  • 7. Belkhir K, Borsa P, Chikhi L, Goudet J, Bonhomme F (1996): GENETIX 4.00 Windows TM Software for Population Genetics, Laboratoire Genome, Populations, Interactions. University of Montpellier, France.
  • 8. Bjórnstad G, Gunby E, Røed KH (2000): Genetic structure of Norwegian horse breeds. J Anim Breed Genet, 117, 307-317.
  • 9. Bowling AT (2001): Historical development and application of molecular genetic tests for horse identification and parentage control. Livest Prod Sci, 72, 111-116.
  • 10. Cozzi MC, Strillacci MG, Valiati P, Bighignoli B, Cancedda M, Zanotti M (2004): Mitochondrial D-loop sequence variation among Italian horse breeds. Genet Sel Evol, 36, 663-672.
  • 11. Dove P, Kavar T, Sölkner H, Achmann R (2006): Development of the Lipizzan Horse Breed. Reprod Dom Anim, 41, 280-285.
  • 12. Erdağ B, Aktopraklıgil D, Koban E, Aksu S, Balcıoğlu K, Aslan Ö, Denizci M, Turgut G, Bağış H, Arat S (2008): A preliminary genetic analysis of some Turkish horse breeds and implications for breed management studies. The Evolutionary Biology Meeting. Marsilya, Fransa.
  • 13. Erdoğan M, Uğuz C, Kopar A, Özbeyaz C (2009): Genetic variability among Arabian horses in Turkey. Kafkas Univ Vet Fak Derg, 15, 267-272.
  • 14. Evanno G, Regnaut S, Goudet J (2005): Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol, 14, 2611– 2620.
  • 15. Glowatzki-Mullis ML, Muntwyler J, Pfister W, Marti E, Rieder S, Poncet PA, Gaillard C (2005): Genetic diversity among horse populations with a special focus on the Franches-Montagnes breed. Anim Genet, 37, 33-39.
  • 16. Gupta AK, Chauhan M, Bhardwaj A, Tandon SN (2012): Microsatellite markers based genetic diversity and bottleneck studies in Zanskari pony. Gene, 499, 357-361.
  • 17. Gupta AK, Chauhan M, Bhardwaj A, Gupta N, Gupta SC, Pal Y, Tandon SN, Vijh RK (2014): Comparative genetic diversity analysis among six Indian breeds and English Thoroughbred horses. Livest Sci, 163, 1-11.
  • 18. Güleç E (1996): Türk Rahvan Atı ve Atçılığı. ISBN: 975- 95931-5-7, Ankara.
  • 19. ISAG/FAO (2004): Secondary Guidelines. Measurement of Domestic Animal Diversity (MoDAD): Recommended Microsatellite Markers.
  • 20. Kavar T, Brem G, Habe F, Sölkner J, Dovc P (2002): History of Lipizzan horse maternal lines as revealed by mtDNA analysis. Genet Sel Evol, 34, 635-648.
  • 21. Lee SY, Cho GJ (2006): Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. J Vet Sci, 7, 63-67.
  • 22. Luis C, Juras R, Oom MM, Cothran EG (2007): Genetic diversity and relationships of Portuguese and other horse breeds based on protein and microsatellite loci variation. Anim Genet, 38, 20-27.
  • 23. Mahrous KF, Hassanane M, Mordy MA, Shafey HI, Hassan N (2011): Genetic variations in horse using microsatellite markers. J Genet Eng Biotechnol, 9, 103- 109.
  • 24. Meira CT, Curi RA, Silva JAIIV, Corrêa MJM, Oliveira de HN, Mota da MDS (2012): Morphological and genomic differences between cutting and racing lines of Quarter horses. JEVS, 1-6.
  • 25. Nei M (1972): Genetic Distance Between Populations. Am Nat, 106, 283-292.
  • 26. Nei M (1987): Molecular Evolutionary Genetics. Columbia University Press, New York.
  • 27. Poncet PA, Pfister W, Muntwyler J, Glowatzki-Mullis ML, Gaillard C (2006): Analysis of pedigree and conformation data to explain genetic variability of the horse breed Franches-Montagnes. J Anim Breed Genet, 123, 114-121.
  • 28. Pritchard K, Stephens M, Donnelly P (2000): Inference of population structure using multilocus genotype data. Genetics, 155, 945-959.
  • 29. Stachurska A, Nogaj A, Brodacki A, Nogaj J, Batkowska J (2014): Genetic distances between horse breeds in Poland estimated according to blood protein polymorphism. Czech J Anim Sci, 59, 257-267.
  • 30. Weir BS, Cockerham CC (1984): Estimating F-statistics for the Analysis of Population. Evolution, 38, 1358-1370.
  • 31. Wright S (1978): The Theory of Gene Frequencies, Evolution and The Genetics of Populations. University of Chicago Press, Vol. 4.
  • 32. Yang YH, Kim KI, Cothran EG, Flannery AR (2002): Genetic Diversity of Cheju Horses (Equus caballus) Determined by Using Mitochondrial DNA D-loop Polymorphism. Biochem Genet, 40, 175-186.
  • 33. Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997): POPGENE, The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Edmonton, Canada.

Türkiye’de rahvan koşan atlar arasındaki genetik çeşitlilik

Yıl 2016, Cilt: 63 Sayı: 2, 201 - 210, 01.06.2016

Banu YÜCEER Metin ERDOĞAN Cevdet YARALI Bora ÖZARSLAN Ceyhan ÖZBEYAZ

https://doi.org/10.1501/Vetfak_0000002730

Öz

Bu araştırma, Türkiye’de 7 farklı coğrafi bölgede yetiştirilen ve Rahvan koşularına katılan atların 17 mikrosatelit lokus bakımından genetik yapılarının belirlenmesi amacıyla yapılmıştır. Bunun için 4 yaş ve üzeri 353 rahvan atın kan/kıl örnekleri kullanılmıştır. Ayrıca, Rahvan koşularına katılan atlar ile Gıda, Tarım ve Hayvancılık Bakanlığı, Yüksek Komiserler Kurulu’na kayıtlı bulunan Arap (n=53) ve İngiliz (n=51) atları arasındaki genetik benzerlik veya farklılıklar da ortaya konulmuştur. Genetik analizler sonucunda, ortalama FIS, FIT ve FST değerleri sırasıyla 0.0982, 0.0999 ve 0.0019; bölgelere göre (İç Anadolu, Karadeniz, Ege, Marmara, Doğu Anadolu, Güneydoğu Anadolu ve Akdeniz Bölgesi) gözlenen heterozigotluk indeksi (Ho) sırasıyla, 0.718, 0.688, 0.711, 0.719, 0.722, 0.700 ve 0.708 olarak belirlenmiştir. Rahvan, Arap ve İngiliz atı populasyonlarındaki ortalama allel sayıları sırasıyla, 10.65, 5.18 ve 5.00 olarak hesaplanmıştır. Populasyonlar arasındaki farklılıkları ortaya çıkarmak amacıyla yapılan Faktöriyel Benzerlik Analizi’nde Rahvan, Arap ve İngiliz atlarının ayrı kümeler oluşturduğu görülmüştür. Aynı sonuçlar yapı (structure) analizinde de ortaya çıkmıştır. Sonuç olarak, Rahvan atlarının bölgelere göre önemli farklılıklar göstermediği ancak, Arap ve İngiliz atlarından ise oldukça farklı oldukları ortaya konmuştur

Anahtar Kelimeler

At, genetik çeşitlilik, mikrosatelit, rahvan, Türkiye

Kaynakça

  • 1. Aberle KS, Hamann H, Drögemüller C, Distl O (2004): Genetic diversity in German draught horse breeds compared with a group of primitive, riding and wild horses by means of microsatellite DNA markers. Anim Genet, 35, 270-277.
  • 2. Achmann R, Curik I, Dovc P, Kavar T, Bodo I, Habe F, Marti E, Sölkner J, Brem G (2004): Microsatellite diversity, population subdivision and gene flow in the Lipizzan horse. Anim Genet, 35, 285-292.
  • 3. Anonim (2014b): QIAamp DNA Mini Kit. Erişim Adresi: www.qiagen.com/products/catalog/sampletechnologies/dna-sample-technologies/genomic-dna/ qiaamp-dna-mini-kit Erişim Tarihi: 07.08.2014
  • 4. Apostolidis AP, Mamuris Z, Karkavelia E, Alifakiotis T (2001): Comparison of Greek breeds of horses using RAPD markers. J Anim Breed Genet, 118, 47-56.
  • 5. Barcaccia G, Felicetti M, Galla G, Capomaccio S, Cappelli K, Albertini E, Buttazzoni L, Pieramati C, Silvestrelli M, Supplizi AV (2013): Molecular analysis of genetic diversity, population structure and inbreeding level of the Italian Lipizzan horse. Livest Sci, 151, 124-133.
  • 6. Batu S (1962): Türk Atları ve At Yetiştirme Bilgisi, Ankara.
  • 7. Belkhir K, Borsa P, Chikhi L, Goudet J, Bonhomme F (1996): GENETIX 4.00 Windows TM Software for Population Genetics, Laboratoire Genome, Populations, Interactions. University of Montpellier, France.
  • 8. Bjórnstad G, Gunby E, Røed KH (2000): Genetic structure of Norwegian horse breeds. J Anim Breed Genet, 117, 307-317.
  • 9. Bowling AT (2001): Historical development and application of molecular genetic tests for horse identification and parentage control. Livest Prod Sci, 72, 111-116.
  • 10. Cozzi MC, Strillacci MG, Valiati P, Bighignoli B, Cancedda M, Zanotti M (2004): Mitochondrial D-loop sequence variation among Italian horse breeds. Genet Sel Evol, 36, 663-672.
  • 11. Dove P, Kavar T, Sölkner H, Achmann R (2006): Development of the Lipizzan Horse Breed. Reprod Dom Anim, 41, 280-285.
  • 12. Erdağ B, Aktopraklıgil D, Koban E, Aksu S, Balcıoğlu K, Aslan Ö, Denizci M, Turgut G, Bağış H, Arat S (2008): A preliminary genetic analysis of some Turkish horse breeds and implications for breed management studies. The Evolutionary Biology Meeting. Marsilya, Fransa.
  • 13. Erdoğan M, Uğuz C, Kopar A, Özbeyaz C (2009): Genetic variability among Arabian horses in Turkey. Kafkas Univ Vet Fak Derg, 15, 267-272.
  • 14. Evanno G, Regnaut S, Goudet J (2005): Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol, 14, 2611– 2620.
  • 15. Glowatzki-Mullis ML, Muntwyler J, Pfister W, Marti E, Rieder S, Poncet PA, Gaillard C (2005): Genetic diversity among horse populations with a special focus on the Franches-Montagnes breed. Anim Genet, 37, 33-39.
  • 16. Gupta AK, Chauhan M, Bhardwaj A, Tandon SN (2012): Microsatellite markers based genetic diversity and bottleneck studies in Zanskari pony. Gene, 499, 357-361.
  • 17. Gupta AK, Chauhan M, Bhardwaj A, Gupta N, Gupta SC, Pal Y, Tandon SN, Vijh RK (2014): Comparative genetic diversity analysis among six Indian breeds and English Thoroughbred horses. Livest Sci, 163, 1-11.
  • 18. Güleç E (1996): Türk Rahvan Atı ve Atçılığı. ISBN: 975- 95931-5-7, Ankara.
  • 19. ISAG/FAO (2004): Secondary Guidelines. Measurement of Domestic Animal Diversity (MoDAD): Recommended Microsatellite Markers.
  • 20. Kavar T, Brem G, Habe F, Sölkner J, Dovc P (2002): History of Lipizzan horse maternal lines as revealed by mtDNA analysis. Genet Sel Evol, 34, 635-648.
  • 21. Lee SY, Cho GJ (2006): Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. J Vet Sci, 7, 63-67.
  • 22. Luis C, Juras R, Oom MM, Cothran EG (2007): Genetic diversity and relationships of Portuguese and other horse breeds based on protein and microsatellite loci variation. Anim Genet, 38, 20-27.
  • 23. Mahrous KF, Hassanane M, Mordy MA, Shafey HI, Hassan N (2011): Genetic variations in horse using microsatellite markers. J Genet Eng Biotechnol, 9, 103- 109.
  • 24. Meira CT, Curi RA, Silva JAIIV, Corrêa MJM, Oliveira de HN, Mota da MDS (2012): Morphological and genomic differences between cutting and racing lines of Quarter horses. JEVS, 1-6.
  • 25. Nei M (1972): Genetic Distance Between Populations. Am Nat, 106, 283-292.
  • 26. Nei M (1987): Molecular Evolutionary Genetics. Columbia University Press, New York.
  • 27. Poncet PA, Pfister W, Muntwyler J, Glowatzki-Mullis ML, Gaillard C (2006): Analysis of pedigree and conformation data to explain genetic variability of the horse breed Franches-Montagnes. J Anim Breed Genet, 123, 114-121.
  • 28. Pritchard K, Stephens M, Donnelly P (2000): Inference of population structure using multilocus genotype data. Genetics, 155, 945-959.
  • 29. Stachurska A, Nogaj A, Brodacki A, Nogaj J, Batkowska J (2014): Genetic distances between horse breeds in Poland estimated according to blood protein polymorphism. Czech J Anim Sci, 59, 257-267.
  • 30. Weir BS, Cockerham CC (1984): Estimating F-statistics for the Analysis of Population. Evolution, 38, 1358-1370.
  • 31. Wright S (1978): The Theory of Gene Frequencies, Evolution and The Genetics of Populations. University of Chicago Press, Vol. 4.
  • 32. Yang YH, Kim KI, Cothran EG, Flannery AR (2002): Genetic Diversity of Cheju Horses (Equus caballus) Determined by Using Mitochondrial DNA D-loop Polymorphism. Biochem Genet, 40, 175-186.
  • 33. Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997): POPGENE, The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Edmonton, Canada.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA78GK39KR
Bölüm Araştırma Makalesi
Yazarlar

Banu YÜCEER
Ankara Üniversitesi, Veteriner Fakültesi, Zootekni Anabilim Dalı, Ankara

Metin ERDOĞAN
Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Medikal Biyoloji ve Genetik Anabilim Dalı, Afyonkarahisar

Cevdet YARALI
Etlik Veteriner Kontrol Merkez Araştırma Enstitüsü, Genetik Laboratuvarı, Ankara

Bora ÖZARSLAN
Kırıkkale Üniversitesi, Veteriner Fakültesi, Zootekni Anabilim Dalı, Kırıkkale.

Ceyhan ÖZBEYAZ
Ankara Üniversitesi, Veteriner Fakültesi, Zootekni Anabilim Dalı, Ankara

Yayımlanma Tarihi 1 Haziran 2016
Yayınlandığı Sayı Yıl 2016Cilt: 63 Sayı: 2

Kaynak Göster

Bibtex @editöre mektup { auvfd531971, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2016}, volume = {63}, number = {2}, pages = {201 - 210}, doi = {10.1501/Vetfak\_0000002730}, title = {Genetic diversity between rahvan (pacing) horses in Turkey}, key = {cite}, author = {Yüceer, Banu and Erdoğan, Metin and Yaralı, Cevdet and Özarslan, Bora and Özbeyaz, Ceyhan} }
APA Yüceer, B. , Erdoğan, M. , Yaralı, C. , Özarslan, B. & Özbeyaz, C. (2016). Genetic diversity between rahvan (pacing) horses in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 63 (2) , 201-210 . DOI: 10.1501/Vetfak_0000002730
MLA Yüceer, B. , Erdoğan, M. , Yaralı, C. , Özarslan, B. , Özbeyaz, C. "Genetic diversity between rahvan (pacing) horses in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 (2016 ): 201-210 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43515/531971>
Chicago Yüceer, B. , Erdoğan, M. , Yaralı, C. , Özarslan, B. , Özbeyaz, C. "Genetic diversity between rahvan (pacing) horses in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 (2016 ): 201-210
RIS TY - JOUR T1 - Türkiye’de rahvan koşan atlar arasındaki genetik çeşitlilik AU - BanuYüceer, MetinErdoğan, CevdetYaralı, BoraÖzarslan, CeyhanÖzbeyaz Y1 - 2016 PY - 2016 N1 - doi: 10.1501/Vetfak_0000002730 DO - 10.1501/Vetfak_0000002730 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 201 EP - 210 VL - 63 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002730 UR - https://doi.org/10.1501/Vetfak_0000002730 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Genetic diversity between rahvan (pacing) horses in Turkey %A Banu Yüceer , Metin Erdoğan , Cevdet Yaralı , Bora Özarslan , Ceyhan Özbeyaz %T Genetic diversity between rahvan (pacing) horses in Turkey %D 2016 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 63 %N 2 %R doi: 10.1501/Vetfak_0000002730 %U 10.1501/Vetfak_0000002730
ISNAD Yüceer, Banu , Erdoğan, Metin , Yaralı, Cevdet , Özarslan, Bora , Özbeyaz, Ceyhan . "Genetic diversity between rahvan (pacing) horses in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 63 / 2 (Haziran 2016): 201-210 . https://doi.org/10.1501/Vetfak_0000002730
AMA Yüceer B. , Erdoğan M. , Yaralı C. , Özarslan B. , Özbeyaz C. Genetic diversity between rahvan (pacing) horses in Turkey. Ankara Univ Vet Fak Derg. 2016; 63(2): 201-210.
Vancouver Yüceer B. , Erdoğan M. , Yaralı C. , Özarslan B. , Özbeyaz C. Genetic diversity between rahvan (pacing) horses in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2016; 63(2): 201-210.
IEEE B. Yüceer , M. Erdoğan , C. Yaralı , B. Özarslan ve C. Özbeyaz , "Genetic diversity between rahvan (pacing) horses in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 63, sayı. 2, ss. 201-210, Haz. 2016, doi:10.1501/Vetfak_0000002730