Öz
Bu çalışmada toplam 90 aromalı UHT süt örneğinde (Kakolu, çilekli ve muzlu, her birisinden 30 adet olacak şekilde) AFM1 varlığı ve seviyesi ELISA metodu kullanılarak araştırıldı. Sonuçlar, incelenen örneklerin hiçbirisinin AFM1 miktarları açısından Türk Gıda Kodeksi ve Avrupa Birliği Kodeksi sınırlarını (50 ppt; 50 ng/l) aşmadığını gösterdi. Buna rağmen, incelenen örneklerin %75.6'sının (68 örnek) 0.39'dan 26.6 ng/l'ye değişen seviyelerde AFM1 ile kontamine olduğu belirlendi. Sonuçlar, incelenen örneklerde belirlenen AFM1 seviyelerinin kabul edilen sınırların altında olmasına karşın, özellikle çocuklar tarafından fazlaca tercih edilen ve tüketilen aromalı UHT sütlerin AFM1 ile kontamine olmaları durumunda, çocuklarda karsinojenlerin biyotransformasyon mekanizmasının daha yavaş olması nedeniyle, bir halk sağlığı problemi olabileceğini gösterdi
Anahtar Kelimeler
Kaynakça
- 1. Anonymous ( 1996): Food Drug Administration (FDA) Sec. 527.400 whole milk, low fat milk, skim milk– aflatoxin M1 (CPG 7106.210). FDA compliance policy guides. p. 219. DC: FDA, Washington.
- 2. Anonymous (2002): International Agency for Research on Cancer (IARC) Monographs on the evaluation of carcinogenic risks to humans. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. Vol: 82, 169-275.
- 3. Anonymous (2008): Gıda maddelerinde belirli bulaşanların maksimum seviyelerinin belirlenmesi hakkında tebliğ. Resmi Gazete, 17 Mayıs 2008. Sayı: 26879 Başbakanlık Basımevi. Ankara, Turkey
- 4. Anonymous (2009): Focus on Emerging Markets, Tetra Pak Dairy Index A biannual news and information source about the dairy industry, pp 7, Issue 1-June 2009.
- 5. Anonymous (2010): Makroekonomik ve Sektörel Görünüm. Ekonomide Hızlı Büyüme Performansı. Pınar Süt Faaliyet Raporu, pp. 17. http://www.pinar.com.tr/pdf/ sut/pinarsut/pdf/tr8.pdf. The last acces, 21.01.2013.
- 6. Anonymous (2012): MycomonitorTM: Catalog No. 961AFLM01M-96, Santa Ana, USA.
- 7. Battacone G, Nudda A, Cannas A, et al (2003): Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1 . J Dairy Sci, 86, 2667-2675.
- 8. Bhat RV, Vasanthi S (2003): Mycotoxin food safety risks in developing countries. Food Safety in Food Security and Food Trade. Vision 2020 for Food. Agr Environ, Focus 10, 17, pp. 1-2.
- 9. Black RE, Williams SM, Jones IE, et al (2002): Children who avoid drinking cow milk have low dietary calcium intakes and poor bone health. Am J Clin Nutr, 76, 675-680.
- 10. Cano-Sancho G, Marin S, Ramos AJ, et al (2010): Occurence of aflatoksin M1 and exposure assesment in Catalonia (Spain). Rev Iberoam Micol, 27, 130-135.
- 11. Cathey CG, Huang AG, Sarr AB, et al (1994): Development and evaluation of a minicolumn assay for the detection of aflatoxin M1 in milk. J Dairy Sci, 77, 1223- 1231.
- 12. Cavaliere C, Foglia P, Pastorini E, et al (2006): Liquid chromatography/tandem mass spectrometric confirmatory method for determining aflatoxin M1 in cow milk comparison between electrospray and atmospheric pressure photoionization sources. J Chromatogr A, 1101, 69-78.
- 13. Celik TH, Sarımehmetoglu B, Kuplulu O (2005): Aflatoxin M1 contamination in pasteurized milk. Vet Arhiv, 75, 57-65.
- 14. Creppy EE (2002): Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol Lett, 127, 19-28.
- 15. Condon EM, Crepinsek MK, Fox MK ( 2009): School meals: Types of foods offered to and consumed by children at lunch and breakfast. J Am Diet Assoc, 109, 67- 78.
Öz
In this study a total of 90 flavored Ultra High Temperature (UHT) milk samples (cacao, strawberry and banana flavors, 30 samples for each) were analyzed in order to determine the occurrence and the level of Aflatoxin M1 (AFM1)contamination by using Enzyme Linked Immuno-Sorbent Assay (ELISA) method. The results showed that none of the samples exceeded the maximum limit of AFM1 prescribed by Turkish Food Codex (TFC) and European Union Codex (50 ppt; 50ng/l). However, 75.6% of the samples (68 samples) examined were determined to be contaminated with AFM1 at various levels ranging between 0.39 and 26.6 ng/l. The results showed that, although the detected AFM1 levels were lower than the accepted limits, AFMcontamination of flavored UHT milk, especially preferred and consumed by children with high susceptibility due to their slower biotransformation capacity for carcinogens, could be a public health concern
Anahtar Kelimeler
Kaynakça
- 1. Anonymous ( 1996): Food Drug Administration (FDA) Sec. 527.400 whole milk, low fat milk, skim milk– aflatoxin M1 (CPG 7106.210). FDA compliance policy guides. p. 219. DC: FDA, Washington.
- 2. Anonymous (2002): International Agency for Research on Cancer (IARC) Monographs on the evaluation of carcinogenic risks to humans. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. Vol: 82, 169-275.
- 3. Anonymous (2008): Gıda maddelerinde belirli bulaşanların maksimum seviyelerinin belirlenmesi hakkında tebliğ. Resmi Gazete, 17 Mayıs 2008. Sayı: 26879 Başbakanlık Basımevi. Ankara, Turkey
- 4. Anonymous (2009): Focus on Emerging Markets, Tetra Pak Dairy Index A biannual news and information source about the dairy industry, pp 7, Issue 1-June 2009.
- 5. Anonymous (2010): Makroekonomik ve Sektörel Görünüm. Ekonomide Hızlı Büyüme Performansı. Pınar Süt Faaliyet Raporu, pp. 17. http://www.pinar.com.tr/pdf/ sut/pinarsut/pdf/tr8.pdf. The last acces, 21.01.2013.
- 6. Anonymous (2012): MycomonitorTM: Catalog No. 961AFLM01M-96, Santa Ana, USA.
- 7. Battacone G, Nudda A, Cannas A, et al (2003): Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1 . J Dairy Sci, 86, 2667-2675.
- 8. Bhat RV, Vasanthi S (2003): Mycotoxin food safety risks in developing countries. Food Safety in Food Security and Food Trade. Vision 2020 for Food. Agr Environ, Focus 10, 17, pp. 1-2.
- 9. Black RE, Williams SM, Jones IE, et al (2002): Children who avoid drinking cow milk have low dietary calcium intakes and poor bone health. Am J Clin Nutr, 76, 675-680.
- 10. Cano-Sancho G, Marin S, Ramos AJ, et al (2010): Occurence of aflatoksin M1 and exposure assesment in Catalonia (Spain). Rev Iberoam Micol, 27, 130-135.
- 11. Cathey CG, Huang AG, Sarr AB, et al (1994): Development and evaluation of a minicolumn assay for the detection of aflatoxin M1 in milk. J Dairy Sci, 77, 1223- 1231.
- 12. Cavaliere C, Foglia P, Pastorini E, et al (2006): Liquid chromatography/tandem mass spectrometric confirmatory method for determining aflatoxin M1 in cow milk comparison between electrospray and atmospheric pressure photoionization sources. J Chromatogr A, 1101, 69-78.
- 13. Celik TH, Sarımehmetoglu B, Kuplulu O (2005): Aflatoxin M1 contamination in pasteurized milk. Vet Arhiv, 75, 57-65.
- 14. Creppy EE (2002): Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol Lett, 127, 19-28.
- 15. Condon EM, Crepinsek MK, Fox MK ( 2009): School meals: Types of foods offered to and consumed by children at lunch and breakfast. J Am Diet Assoc, 109, 67- 78.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Diğer ID | JA82NK67FZ |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Eylül 2015 |
| Yayımlandığı Sayı | Yıl 2015Cilt: 62 Sayı: 3 |