Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea

Yıl 2015, Cilt: 62 Sayı: 1, 17 - 21, 01.03.2015

Serpil KAHYA Kaan ÖNAT Evren ERKÖSE Seran TEMELLİ Aysegul EYİGOR Kamil Tayfun CARLI

https://doi.org/10.1501/Vetfak_0000002652

Öz

Aim of this study was to determine if Mentofin would have any effect on Mycoplasma gallisepticum (MG) clearance from the tracheal epithelium of chickens in commercial layer flocks, which were naturally infected with MG. Results indicated that, compared to the control group, there was a significant and continuous decline in MG infection in chickens of Mentofingroup determined by culture and Real-Time Polymerase Chain Reaction (MGrPCR) (P<0,05). Serology results in the control group indicated an increase in MG positivity from 25% to 40% (P>0,05), while there was no change in the Mentofin group (P>0,05). Culture results for MG positivity decreased from 85% to 5% in the Mentofin group, while this decrease was from 80% to 35% in the control group (P<0,05). There was a prominent decrease from 100% to 20% in MGrPCR positives in the Mentofin group (P<0,05) compared to a non-significant change observed from 95% to 80% in the control group (P>0,05). Results of this study indicate that Mentofin clearly had an effect on MG clearance from the tracheal epithelium, supported by detection of decline in MG infection in layers

Anahtar Kelimeler

Chicken, Mentofin, Mycoplasma gallisepticum

Kaynakça

  • Babayi H, Kolo I, Okogun JI, Ijah UJJ (2004): The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biokemistri, 16, 106-111.
  • Barbour EK, Yaghi RH, Shaib HA, Tayeb IT, Sleiman FT (2008): Evaluation of an essential oil in treatment of immunosuppressed-coinfected broilers. Am Eurasian J Sustain Agric, 2, 212-218.
  • Barua SR, Prodhan AM, Islam S, Chowdhury S (2006): Study on Mycoplasma gallisepticum in chickens in selected areas of Bangladesh. J Vet Med Sci, 4, 141-142.
  • Blanchard B, Chevallier B, Vermissey Y, Charbonnier A, Guennec JLE (2001): Detection by PCR of avian Mycoplasmas from environmental swabs of poultry houses. Br Poult Sci, 42, 59-63.
  • Bragg B (2004): Evaluation of the safety of the continual use of the product, Mentofin® for spraying of layer chickens. Evaluation of the safety of the continual use of the product, Mentofin® for spraying of broiler chickens. Available at: downloads/16_08_mentofin_gb_folder.pdf
  • Bragg RR (2006): Determination of the minimum inhibitory concentrations (MIC) of Mentofin® against Newcastle Disease virus with a 15 min contact period. Available at: http://www.ewabo.de/sites/default/files/downloads/16_08_ mentofin_gb_folder.pdf
  • Carli KT, Eyigor A (2003): Real-time polymerase chain reaction for detection of Mycoplasma gallisepticum in chicken trachea. Avian Dis, 47, 712-717.
  • Carli KT, Kahya S (2011): Kanatlı Hayvanların İnfeksiyöz Hastalıkları. Uludağ Üniversitesi Veteriner Fakültesi Yayınları, Bursa.
  • Carli KT, Onat K, Gunaydin E (2008): Application of Mentofin® in broilers with clinical Infectious Bursal Disease to reduce Escherichia coli related problems after vaccination against Newcastle Disease. Turk J Vet Anim Sci, 32, 73-79.
  • Dovc P, Bencina D, Antes R, Mann W (1994): Recombinant DNA probes and polymerase chain reaction for detection of Mycoplasma gallisepticum strains. FEMS Microbiol Lett, 122, 79-84.
  • Esendal OM (2002): Mikoplazma infeksiyonları. 79-95. In: Kanatlı Hayvan Hastalıkları. Medisan Yayın, Ankara.
  • Evans JD, Leigh SA, Branton SL, Collier SD, Pharr GT, Bearson SMD (2005): Mycoplasma gallisepticum: Current and developing means to control the avian pathogen. J Appl Poult Res, 14, 757-763.
  • Fan HH, Kleven H, Jackwood MW, Johansson KE, Petterson B, Levisohn S (1995): Species identification of avian Mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Dis, 39, 398-407.
  • Haaheim H, Vorland L, Gutteberg TJ (2001): Laboratory diagnosis of respiratory diseases: PCR versus serology. Nucleosides, Nucleotides, Nucleic Acids, 20, 1255-1258.
  • Hossain KMM, Ali MY, Hauque MI (2006): Seroprevalence of Mycoplasma gallisepticum infection in chicken in the greater rajshahı strict of Bangladesh. J Vet Med Sci, 5, 9-14.
  • İzgür M (2006): Mycoplasma, Ureaplasma, Acheloplasma, Spiroplasma ve Erysipelothrix infeksiyonları. 293-304. In: Veteriner Mikrobiyoloji (bakteriyel hastalıklar). İlke Emek Matbaacılık ve Yayıncılık, Ankara.
  • Jain P, Nimbrana S, Kaila G (2010): Antimicrobial activity and phytochemical analysis of Eucalyptus tereticornis bark and leaf methanolic extracts. Int J Pharm Sci Rev Res, 4, 126-128.
  • Kahya S, Temelli S, Eyigor A, Çarlı KT (2010): Real- Time PCR, bacteriology and serology for the diagnosis of Mycoplasma gallisepticum in chicken breeder flocks. Vet Microbiol, 144, 319-324.
  • Kleven SH, Yoder HW (1989): Mycoplasmosis: A Laboratory Manual for the Isolation and Identification of Avian Pathogens. Pages 57–62 in: American Association of Avian Pathologists. 3rd ed. H. G. Purchase, L. H. Arp, C. H. Domermuth, and J. E. Pearson, ed. Kennett Square, PA, USA.
  • Ley DH (2003): Mycoplasma gallisepticum infection. 722- 743. In: Diseases of Poultry, Iowa State University Press, IA, USA.
  • Mohamed GA, Ibrahim SRM (2007): Eucalyptone G, A new phloroglucinol derivative and other costituents from Eucalyptus globulus Labill. Arkıvoc, XV, 281-291.
  • Mohammed HO, Carpenter TE, Yamamoto R (1987): Economic impact of Mycoplasma gallisepticum and Mycoplasma synoviae in commercial layer flocks. Avian Dis, 31, 477-82.
  • Nair R, Vaghasiya Y, Chanda S (2008): Antibacterial activity of Eucalpytus citriodora Hk. oil on few clinically important bacteria. Afr J Biotechnol, 7, 25-26.
  • Navarro V, Villarreal ML, Rojas G, Lozoya X (1996): Antimicrobial evaluation of some plants used in Mexican traditional medicine for the treatment of infectious diseases. J Ethnopharmacol, 53, 143-147.
  • Poveda J.B (1998): Biochemical Charactristics in Mycoplasma Identification. 69-78. In: Methods in Molecular Biology. Mycoplasma Protocols. Humana Press Inc, Totowa, NJ, USA.
  • Sarkar SK, Rahman M, Amin KMR, Khan MFR, Rahman MM (2005): Seroprevalence of Mycoplasma gallisepticum infection of chickens in model breeder poultry farms of Bangladesh. Int J Poult Sci, 4, 32-35.
  • Sikder AJ, Islam MA, Rahman MM, Rahman MB (2005): Seroprevalence of Salmonella and Mycoplasma gallisepticum infection in the six model breeder poultry farms at Patuakhali district in Bangladesh. Int J Poult Sci, 4, 905-910.
  • United States Department of Agriculture (2011): Standart test procedures for Mycoplasma. Subpart A, Section 147-7. National Poultry Improvement Plan and Auxiliary Provisions, USDA, Animal and Plant Health Inspection Service.
  • World Organization for Animal Health (2013): Avian Mycoplasmosis (Mycoplasma gallisepticum, M. synoviae). Chapter 2.3.5. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
  • Yoder HW (1984): Mycoplasma gallisepticum infection. 190-212. In: Diseases of Poultry. Iowa State University Press, Iowa, IA, USA.

Mentofin uygulamasının Mycoplasma gallisepticum (MG) ile doğal infekte yumurtacı tavuklarıntrakeasından arınması üzerine etkisi

Yıl 2015, Cilt: 62 Sayı: 1, 17 - 21, 01.03.2015

Serpil KAHYA Kaan ÖNAT Evren ERKÖSE Seran TEMELLİ Aysegul EYİGOR Kamil Tayfun CARLI

https://doi.org/10.1501/Vetfak_0000002652

Öz

Bu çalışmanın amacı Mentofin’in Mycoplasma gallisepticum (MG) ile doğal infekte ticari yumurtacı sürülerin trakeal epitellerinden MG’un arınması üzerine etkisinin belirlenmesidir. Sonuçlar, kontrol grubu ile karşılaştırıldığında Mentofin grubundaki tavuklarda MG infeksiyonunda kültür ve Real-Time Polymerase Chain Reaction (MGrPCR) ile belirlenen belirgin ve sürekli bir düşüş olduğunu göstermiştir (P<0,05). Seroloji sonuçları kontrol grubunda MG pozitiflik %25’den %40’a yükselirken (P>0,05), Mentofin grubunda bir değişiklik olmamıştır (P>0,05). Kültür sonuçlarındaki MG pozitiflik Mentofin grubunda %85’den %5’e düşerken, kontrol grubunda bu düşüş %80’den %35’e olmuştur (P<0,05). Mentofin grubundaki MGrPCR pozitifliğinde belirgin şekilde olan %100’den %20’ye düşüş (P<0,05), kontrol grubunda %95’den %80’e (P>0,05) olan hafif bir düşme olarak gözlenmiştir. Çalışma sonuçları Mentofin’in trakeal epitelden MG arınmasında belirgin bir etkisinin olduğunu yumurtacılarda MG infeksiyonunda düşmenin belirlenmesi ile desteklenen şekilde göstermiştir

Anahtar Kelimeler

Mentofin, Mycoplasma gallisepticum, tavuk

Kaynakça

  • Babayi H, Kolo I, Okogun JI, Ijah UJJ (2004): The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biokemistri, 16, 106-111.
  • Barbour EK, Yaghi RH, Shaib HA, Tayeb IT, Sleiman FT (2008): Evaluation of an essential oil in treatment of immunosuppressed-coinfected broilers. Am Eurasian J Sustain Agric, 2, 212-218.
  • Barua SR, Prodhan AM, Islam S, Chowdhury S (2006): Study on Mycoplasma gallisepticum in chickens in selected areas of Bangladesh. J Vet Med Sci, 4, 141-142.
  • Blanchard B, Chevallier B, Vermissey Y, Charbonnier A, Guennec JLE (2001): Detection by PCR of avian Mycoplasmas from environmental swabs of poultry houses. Br Poult Sci, 42, 59-63.
  • Bragg B (2004): Evaluation of the safety of the continual use of the product, Mentofin® for spraying of layer chickens. Evaluation of the safety of the continual use of the product, Mentofin® for spraying of broiler chickens. Available at: downloads/16_08_mentofin_gb_folder.pdf
  • Bragg RR (2006): Determination of the minimum inhibitory concentrations (MIC) of Mentofin® against Newcastle Disease virus with a 15 min contact period. Available at: http://www.ewabo.de/sites/default/files/downloads/16_08_ mentofin_gb_folder.pdf
  • Carli KT, Eyigor A (2003): Real-time polymerase chain reaction for detection of Mycoplasma gallisepticum in chicken trachea. Avian Dis, 47, 712-717.
  • Carli KT, Kahya S (2011): Kanatlı Hayvanların İnfeksiyöz Hastalıkları. Uludağ Üniversitesi Veteriner Fakültesi Yayınları, Bursa.
  • Carli KT, Onat K, Gunaydin E (2008): Application of Mentofin® in broilers with clinical Infectious Bursal Disease to reduce Escherichia coli related problems after vaccination against Newcastle Disease. Turk J Vet Anim Sci, 32, 73-79.
  • Dovc P, Bencina D, Antes R, Mann W (1994): Recombinant DNA probes and polymerase chain reaction for detection of Mycoplasma gallisepticum strains. FEMS Microbiol Lett, 122, 79-84.
  • Esendal OM (2002): Mikoplazma infeksiyonları. 79-95. In: Kanatlı Hayvan Hastalıkları. Medisan Yayın, Ankara.
  • Evans JD, Leigh SA, Branton SL, Collier SD, Pharr GT, Bearson SMD (2005): Mycoplasma gallisepticum: Current and developing means to control the avian pathogen. J Appl Poult Res, 14, 757-763.
  • Fan HH, Kleven H, Jackwood MW, Johansson KE, Petterson B, Levisohn S (1995): Species identification of avian Mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Dis, 39, 398-407.
  • Haaheim H, Vorland L, Gutteberg TJ (2001): Laboratory diagnosis of respiratory diseases: PCR versus serology. Nucleosides, Nucleotides, Nucleic Acids, 20, 1255-1258.
  • Hossain KMM, Ali MY, Hauque MI (2006): Seroprevalence of Mycoplasma gallisepticum infection in chicken in the greater rajshahı strict of Bangladesh. J Vet Med Sci, 5, 9-14.
  • İzgür M (2006): Mycoplasma, Ureaplasma, Acheloplasma, Spiroplasma ve Erysipelothrix infeksiyonları. 293-304. In: Veteriner Mikrobiyoloji (bakteriyel hastalıklar). İlke Emek Matbaacılık ve Yayıncılık, Ankara.
  • Jain P, Nimbrana S, Kaila G (2010): Antimicrobial activity and phytochemical analysis of Eucalyptus tereticornis bark and leaf methanolic extracts. Int J Pharm Sci Rev Res, 4, 126-128.
  • Kahya S, Temelli S, Eyigor A, Çarlı KT (2010): Real- Time PCR, bacteriology and serology for the diagnosis of Mycoplasma gallisepticum in chicken breeder flocks. Vet Microbiol, 144, 319-324.
  • Kleven SH, Yoder HW (1989): Mycoplasmosis: A Laboratory Manual for the Isolation and Identification of Avian Pathogens. Pages 57–62 in: American Association of Avian Pathologists. 3rd ed. H. G. Purchase, L. H. Arp, C. H. Domermuth, and J. E. Pearson, ed. Kennett Square, PA, USA.
  • Ley DH (2003): Mycoplasma gallisepticum infection. 722- 743. In: Diseases of Poultry, Iowa State University Press, IA, USA.
  • Mohamed GA, Ibrahim SRM (2007): Eucalyptone G, A new phloroglucinol derivative and other costituents from Eucalyptus globulus Labill. Arkıvoc, XV, 281-291.
  • Mohammed HO, Carpenter TE, Yamamoto R (1987): Economic impact of Mycoplasma gallisepticum and Mycoplasma synoviae in commercial layer flocks. Avian Dis, 31, 477-82.
  • Nair R, Vaghasiya Y, Chanda S (2008): Antibacterial activity of Eucalpytus citriodora Hk. oil on few clinically important bacteria. Afr J Biotechnol, 7, 25-26.
  • Navarro V, Villarreal ML, Rojas G, Lozoya X (1996): Antimicrobial evaluation of some plants used in Mexican traditional medicine for the treatment of infectious diseases. J Ethnopharmacol, 53, 143-147.
  • Poveda J.B (1998): Biochemical Charactristics in Mycoplasma Identification. 69-78. In: Methods in Molecular Biology. Mycoplasma Protocols. Humana Press Inc, Totowa, NJ, USA.
  • Sarkar SK, Rahman M, Amin KMR, Khan MFR, Rahman MM (2005): Seroprevalence of Mycoplasma gallisepticum infection of chickens in model breeder poultry farms of Bangladesh. Int J Poult Sci, 4, 32-35.
  • Sikder AJ, Islam MA, Rahman MM, Rahman MB (2005): Seroprevalence of Salmonella and Mycoplasma gallisepticum infection in the six model breeder poultry farms at Patuakhali district in Bangladesh. Int J Poult Sci, 4, 905-910.
  • United States Department of Agriculture (2011): Standart test procedures for Mycoplasma. Subpart A, Section 147-7. National Poultry Improvement Plan and Auxiliary Provisions, USDA, Animal and Plant Health Inspection Service.
  • World Organization for Animal Health (2013): Avian Mycoplasmosis (Mycoplasma gallisepticum, M. synoviae). Chapter 2.3.5. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
  • Yoder HW (1984): Mycoplasma gallisepticum infection. 190-212. In: Diseases of Poultry. Iowa State University Press, Iowa, IA, USA.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA49UD25ZS
Bölüm Araştırma Makalesi
Yazarlar

Serpil KAHYA
Uludag University, Faculty of Veterinary Medicine, Department of Microbiology, Bursa

Kaan ÖNAT
Ministry of Food, Agriculture and Livestock, Balıkesir; Uludag University Faculty of Veterinary Medicine

Evren ERKÖSE
Department of Food Hygiene and Technology, Bursa, Turkey

Seran TEMELLİ
Department of Food Hygiene and Technology, Bursa, Turkey

Aysegul EYİGOR
Department of Food Hygiene and Technology, Bursa, Turkey

Kamil Tayfun CARLI
Uludag University, Faculty of Veterinary Medicine, Department of Microbiology, Bursa

Yayımlanma Tarihi 1 Mart 2015
Yayınlandığı Sayı Yıl 2015Cilt: 62 Sayı: 1

Kaynak Göster

Bibtex @editöre mektup { auvfd532428, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2015}, volume = {62}, number = {1}, pages = {17 - 21}, doi = {10.1501/Vetfak\_0000002652}, title = {Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea}, key = {cite}, author = {Kahya, Serpil and Önat, Kaan and Erköse, Evren and Temelli, Seran and Eyigor, Aysegul and Carlı, Kamil Tayfun} }
APA Kahya, S. , Önat, K. , Erköse, E. , Temelli, S. , Eyigor, A. & Carlı, K. T. (2015). Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 62 (1) , 17-21 . DOI: 10.1501/Vetfak_0000002652
MLA Kahya, S. , Önat, K. , Erköse, E. , Temelli, S. , Eyigor, A. , Carlı, K. T. "Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 62 (2015 ): 17-21 <http://vetjournal.ankara.edu.tr/tr/pub/issue/43549/532428>
Chicago Kahya, S. , Önat, K. , Erköse, E. , Temelli, S. , Eyigor, A. , Carlı, K. T. "Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea". Ankara Üniversitesi Veteriner Fakültesi Dergisi 62 (2015 ): 17-21
RIS TY - JOUR T1 - Mentofin uygulamasının Mycoplasma gallisepticum (MG) ile doğal infekte yumurtacı tavuklarıntrakeasından arınması üzerine etkisi AU - SerpilKahya, KaanÖnat, EvrenErköse, SeranTemelli, AysegulEyigor, Kamil TayfunCarlı Y1 - 2015 PY - 2015 N1 - doi: 10.1501/Vetfak_0000002652 DO - 10.1501/Vetfak_0000002652 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 17 EP - 21 VL - 62 IS - 1 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002652 UR - https://doi.org/10.1501/Vetfak_0000002652 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea %A Serpil Kahya , Kaan Önat , Evren Erköse , Seran Temelli , Aysegul Eyigor , Kamil Tayfun Carlı %T Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea %D 2015 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 62 %N 1 %R doi: 10.1501/Vetfak_0000002652 %U 10.1501/Vetfak_0000002652
ISNAD Kahya, Serpil , Önat, Kaan , Erköse, Evren , Temelli, Seran , Eyigor, Aysegul , Carlı, Kamil Tayfun . "Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea". Ankara Üniversitesi Veteriner Fakültesi Dergisi 62 / 1 (Mart 2015): 17-21 . https://doi.org/10.1501/Vetfak_0000002652
AMA Kahya S. , Önat K. , Erköse E. , Temelli S. , Eyigor A. , Carlı K. T. Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea. Ankara Univ Vet Fak Derg. 2015; 62(1): 17-21.
Vancouver Kahya S. , Önat K. , Erköse E. , Temelli S. , Eyigor A. , Carlı K. T. Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2015; 62(1): 17-21.
IEEE S. Kahya , K. Önat , E. Erköse , S. Temelli , A. Eyigor ve K. T. Carlı , "Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 62, sayı. 1, ss. 17-21, Mar. 2015, doi:10.1501/Vetfak_0000002652