Sığır, koyun ve insanlardan izole edilen Brucella suşlarının konvansiyonel ve moleküler biyotiplendirmesi
Öz
Bu çalışmada Kayseri bölgesinde sığır ve koyunlardaki atık olgularında Brucella’nın rolü araştırıldı ve ağırlıklı alt-tür ve biyovarlar konvansiyonel ve moleküler biyotiplendirme metotları ile belirlendi. Çalışmada 61 adet sığır, 64 adet koyun atık materyali ve ayrıca 50 insan kanından izole edilmiş Brucella spp. incelendi. On yedi (%27.9)’si sığır ve 12’si (%18.7) koyun atık örneklerinden olmak üzere toplam 29 adet Brucella spp. izole edildi. Hayvan ve insan izolatları konvansiyonel yöntemler ve Enhanced AMOS-ERY PCR olmak üzere iki farklı yöntem ile tiplendirildi. Çalışma kapsamında sığırlardan izole edilen tüm Brucella spp.’nin B.abortus biyovar 3’e ait olduğu konvansiyonel yöntemlerle saptandı ve bu izolatların tamamının moleküler olarak biyovar 3b olduğu tespit edildi. Koyun ve insan orijinli izolatların ise konvansiyonel ve moleküler olarak B.melitensis biyovar 3 oldukları tespit edildi. Bu çalışma ile Türkiye’nin Kayseri İli’nde, insan, sığır ve koyunlarda Brucellosis’e neden olan predominant Brucella biovarları belirlenmiş oldu. Bu bulguların Brucellosise yönelik koruma ve kontrol aktiviteleri için yararlı olabileceği düşünülmektedir
Anahtar Kelimeler
Kaynakça
- Al-Ani FK, Qaderi NQ, Razziq R, Al-Darraji AM (2004): Human and animal brucellosis in Jordan between 1996–1998: a study. Rev Sci Tech Off Int Epizoot, 23, 831–840.
- Alton GG, Jones LM, Pietz DE (1975): Laboratory Techniques in Brucellosis. Monograph Series. World Health Organization, Geneva, Switzerland.
- Anon (2011): WHO, Brucellosis, http://www.who.int/ zoonoses/diseases/ Brucellosissurveillance.pdf.
- Bolca Z, Gündeş S, Erdenliğ S, Özturk R, Sümerkan B, Akata, F, Vahaboğlu, H (2002): İnsan kaynaklı brusella türü mikroorganizmaların tiplendirilmesi amacı ile uygulanan metotların karşılaştırılması ve biyotipleri ile faj tipleri arasındaki ilişkinin irdelenmesi. Flora, 7(3), 157– 170.
- Bricker BJ (2002a): Diagnostic Strategies Used for the Identification of Brucella. Vet Microbiol, 90, 433–434.
- Bricker BJ (2002b): PCR as a Diagnostic Tool for Brucellosis. Vet Microbiol, 90, 435–446.
- Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus by 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660–2666.
- Bricker BJ, Halling SM (1995): Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J Clin Microbiol, 33, 1640–1642.
- Buzgan T, Karahocagil MK, Irmak H (2010): Clinical manifestations and complications in 1028 cases of brucellosis: a retrospective evaluation and review of the literature. Int J Infect Dis, 14, 469–478.
- Büyük, F, Şahin M (2011): Invesigation of Brucella species from various samples of aborted cattle in Kars Province (Turkey) by cultural and molecular methods and epidemiological analysis of cases, The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (5), 809–816.
- Büyükcangaz E, Şen A, Kahya S (2009): Isolation and Biotyping of Brucella melitensis from Sheep and Goat Aborted Fetuses. Turk J Vet Anim Sci, 33, 311–316.
- Çelebi Ö, Otlu S (2011): Bacteriological and Molecular Description of Brucella SpeciesIsolated from Milk and Vaginal Swab Samples of Aborted Cattlein Kars Region. The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (1), 53–58.
- Doğanay M, Aygen B (2003): Human Brucellosis: an overview, Int J Infect Dis, 7, 173–182.
- Erdenliğ S, Sen A (2000): Isolation and biotyping of Brucella species in aborted sheep fetuses. J Pendik Vet Microbiol, 31, 31–42.
- Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol, 93, 53–61.
- Gülhan, T., Aksakal, A, Ekin, İH, Boynukara B (2011): Retrospective Evaluation of Examined Materials for Diagnosis in University Yuzuncu Yil Faculty of Veterinary Medicine Microbiology Department Laboratory. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 22 (2), 127– 132.
- Ica T, Aydin F, Erdenlig S, Güler L, Büyükcangaz E (2008): Characterisation of Brucella abortus biovar 3 isolates from Turkey as subgroup 3b. Vet Rec, 163, 659- 661.
- Kubuafor DK, Awumbila B, Akanmori BD (2000): Seroprevalence of brucellosis in cattle and humans in the Akwapim-South district of Gahana: public health implications. Acta Tropica, 76, 45–48.
- Meyer ME (1976): Evolution and taxonomy in the genus Brucella: progesterone induction of filterable forms of Brucella abortus type 2 with revertant characteristics essentially indistinguishable in vitro from those of Brucella ovis. Am J Vet Res, 37, 211–214.
- Ocampo-Sosa AA, BalbIin JA, García-Lobo JM (2005): Development of a new PCR assay to identify Brucella abortus biovars 5, 6 and 9 and the new subgroup 3b of biovar 3. Vet Microbiol, 110, 41–51.
- Refai M (2002): Incidence and control of brucellosis in the Near East region. Vet Microbiol, 90, 81–110.
- Sümerkan B, Gökahmetoglu S, Esel D (2001): Brucella detection in blood: comparison of the BacT/Alert standard aerobic bottle, BacT/Alert FAN aerobic bottle and BacT/Alert enhanced FAN aerobic bottle in simulated blood culture. Clin Microbiol Infect, 7, 369–393.
- Şahin M, Genç O, Ünver A, Otlu S (2008): Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Product, 40, 281-286.
- Şimşek H, Erdenlig S, Oral B, Tülek N (2004): Typing- biotyping of Brucella isolates of human origin and their epidemiologic evaluation. Klimik Derg, 17, 103–106.
- Ünver A, Erdoğan HM, Atabay Hİ, Şahin M, Çelebi Ö (2006): characterization of Brucella melitensis from aborted sheep fetuses in Kars, Turkey. Revue Vet Med, 157(1), 42–46.
- Yu WL, Nielsen K (2010): Review of Detection of Brucella spp. by Polymerase Chain Reaction. Croat Med J, 51, 306–313.
Yıl 2012,
Cilt: 59 Sayı: 4, 259 - 264, 01.12.2012
Öz
In this study, the role of Brucella spp. in cattle and sheep abortions among Kayseri region was investigated and predominant subspecies and biovars in this region were determined by conventional and molecular biotyping methods. For this purpose, 61 cattle and 64 sheep abortion material and also 50 human blood isolates were examined. A total of 29 Brucella spp. 17 (27.9%) and 12 (18.7%) of which were isolated from cattle and sheep specimens, respectively) were isolated from animal sources. Both animal and human isolates were typed by conventional and Enhanced AMOS-ERY PCR methods. All Brucella spp. strains isolated from cattle were found to belong to B. abortus biovar 3 and biovar 3b using conventional and molecular typing methods, respectively. All sheep originated Brucella spp. strains and human originated Brucella spp. strains were found to belong to B.melitensis biovar 3 using both conventional and molecular methods. As a result, predominant biovars causing brucellosis in human, cattle and sheep in Kayseri, Turkey were detected. These findings were considered to be useful in prevention and controlling activities for Brucellosis in Turkey
Anahtar Kelimeler
Kaynakça
- Al-Ani FK, Qaderi NQ, Razziq R, Al-Darraji AM (2004): Human and animal brucellosis in Jordan between 1996–1998: a study. Rev Sci Tech Off Int Epizoot, 23, 831–840.
- Alton GG, Jones LM, Pietz DE (1975): Laboratory Techniques in Brucellosis. Monograph Series. World Health Organization, Geneva, Switzerland.
- Anon (2011): WHO, Brucellosis, http://www.who.int/ zoonoses/diseases/ Brucellosissurveillance.pdf.
- Bolca Z, Gündeş S, Erdenliğ S, Özturk R, Sümerkan B, Akata, F, Vahaboğlu, H (2002): İnsan kaynaklı brusella türü mikroorganizmaların tiplendirilmesi amacı ile uygulanan metotların karşılaştırılması ve biyotipleri ile faj tipleri arasındaki ilişkinin irdelenmesi. Flora, 7(3), 157– 170.
- Bricker BJ (2002a): Diagnostic Strategies Used for the Identification of Brucella. Vet Microbiol, 90, 433–434.
- Bricker BJ (2002b): PCR as a Diagnostic Tool for Brucellosis. Vet Microbiol, 90, 435–446.
- Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus by 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660–2666.
- Bricker BJ, Halling SM (1995): Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J Clin Microbiol, 33, 1640–1642.
- Buzgan T, Karahocagil MK, Irmak H (2010): Clinical manifestations and complications in 1028 cases of brucellosis: a retrospective evaluation and review of the literature. Int J Infect Dis, 14, 469–478.
- Büyük, F, Şahin M (2011): Invesigation of Brucella species from various samples of aborted cattle in Kars Province (Turkey) by cultural and molecular methods and epidemiological analysis of cases, The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (5), 809–816.
- Büyükcangaz E, Şen A, Kahya S (2009): Isolation and Biotyping of Brucella melitensis from Sheep and Goat Aborted Fetuses. Turk J Vet Anim Sci, 33, 311–316.
- Çelebi Ö, Otlu S (2011): Bacteriological and Molecular Description of Brucella SpeciesIsolated from Milk and Vaginal Swab Samples of Aborted Cattlein Kars Region. The Journal of the Faculty of Veterinary Medicine, University of Kafkas, 17 (1), 53–58.
- Doğanay M, Aygen B (2003): Human Brucellosis: an overview, Int J Infect Dis, 7, 173–182.
- Erdenliğ S, Sen A (2000): Isolation and biotyping of Brucella species in aborted sheep fetuses. J Pendik Vet Microbiol, 31, 31–42.
- Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol, 93, 53–61.
- Gülhan, T., Aksakal, A, Ekin, İH, Boynukara B (2011): Retrospective Evaluation of Examined Materials for Diagnosis in University Yuzuncu Yil Faculty of Veterinary Medicine Microbiology Department Laboratory. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 22 (2), 127– 132.
- Ica T, Aydin F, Erdenlig S, Güler L, Büyükcangaz E (2008): Characterisation of Brucella abortus biovar 3 isolates from Turkey as subgroup 3b. Vet Rec, 163, 659- 661.
- Kubuafor DK, Awumbila B, Akanmori BD (2000): Seroprevalence of brucellosis in cattle and humans in the Akwapim-South district of Gahana: public health implications. Acta Tropica, 76, 45–48.
- Meyer ME (1976): Evolution and taxonomy in the genus Brucella: progesterone induction of filterable forms of Brucella abortus type 2 with revertant characteristics essentially indistinguishable in vitro from those of Brucella ovis. Am J Vet Res, 37, 211–214.
- Ocampo-Sosa AA, BalbIin JA, García-Lobo JM (2005): Development of a new PCR assay to identify Brucella abortus biovars 5, 6 and 9 and the new subgroup 3b of biovar 3. Vet Microbiol, 110, 41–51.
- Refai M (2002): Incidence and control of brucellosis in the Near East region. Vet Microbiol, 90, 81–110.
- Sümerkan B, Gökahmetoglu S, Esel D (2001): Brucella detection in blood: comparison of the BacT/Alert standard aerobic bottle, BacT/Alert FAN aerobic bottle and BacT/Alert enhanced FAN aerobic bottle in simulated blood culture. Clin Microbiol Infect, 7, 369–393.
- Şahin M, Genç O, Ünver A, Otlu S (2008): Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Product, 40, 281-286.
- Şimşek H, Erdenlig S, Oral B, Tülek N (2004): Typing- biotyping of Brucella isolates of human origin and their epidemiologic evaluation. Klimik Derg, 17, 103–106.
- Ünver A, Erdoğan HM, Atabay Hİ, Şahin M, Çelebi Ö (2006): characterization of Brucella melitensis from aborted sheep fetuses in Kars, Turkey. Revue Vet Med, 157(1), 42–46.
- Yu WL, Nielsen K (2010): Review of Detection of Brucella spp. by Polymerase Chain Reaction. Croat Med J, 51, 306–313.
Toplam 26 adet kaynakça vardır.
Ayrıntılar
| Diğer ID | JA23GA38TB |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 2012 |
| Yayımlandığı Sayı | Yıl 2012Cilt: 59 Sayı: 4 |