Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Korunga (Onobrychis Viciifolia) bitkisinin antioksidan etkinliğinin in vitro değerlendirilmesi

Yıl 2012, Cilt: 59 Sayı: 1, 23 - 27, 01.03.2012

Sinan INCE Husamettin EKICI Begum YURDAKOK

https://doi.org/10.1501/Vetfak_0000002496

Öz

Bu çalışmada, korunga bitkisinin (Onobrychis viciifolia) toprak üstü kısımlarının aseton, methanol, etilasetat ve su ekstrelerinin antioksidan etkinliği araştırıldı. Ekstrelerin fenolik içeriği Folin-Ciocalteu yöntemiyle, antioksidan aktivitesinin belirlenmesi fosfomolibden metodu, beta-karoten-linoleat model sistemi, radikal süpürücü etkiye karşı 2,2-difenil-1-pikrilhidrazil (DPPH) ve demirli kompleks olan Fe+3/K3Fe(CN)6 bileşiğinin indirgenmesi ile gerçekleştirildi. Bu çalışma, yüksek antioksidan özelliği gösteren korunga bitkisinin aseton ve metanollü ekstrelerinin, ileride farmasötik çalışmalar için değerlendirilebileceğini önermektedir

Anahtar Kelimeler

Antioksidan aktivite, serbest radikal süpürücü aktivite, O. Viciifolia

Kaynakça

  • Abdille MdH, Singh RP, Jayaprakasha GK, Jena BS (2005): Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem, 90, 891-896.
  • Afolayan AJ, Jimoh FO, Sofidiya MO, Koduru S, Lewu FB (2007): Medicinal potential of the root of Arctotis arctotoides. Pharm Biol, 45, 486-493.
  • Amarowicz R, Piskula M, Honke J, Rudnicka B, Troszynska A, Kozlowska H (1995): Extraction of phenolic compounds from lentil seeds (Lens culinaris) with various solvents. Pol J Food Nutr Sci, 4, 53-62.
  • Amarowicz R, Żegarska Z, Pegg RB, Karamać M, Kosińska A (2006): Antioxidant and radical scavenging activities of a barley crude extract and its fractions. Czech J Food Sci, 25, 73–80.
  • Barrau E, Fabre N, Fouraste I, Hoste H (2005): Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. J Parasitol, 131, 1–8.
  • Bartolome B, Nunez V, Monagas M, Gomez-Cordoves C (2004): In vitro antioxidant activity of red grape skins. Eur Food Res Technol, 218, 173-177.
  • Beninger CW, Hosfield GL (2003): Antioxidant activity extracts, condensed tannin fractions and pure flavonoids from Phaseolus vulgaris L. Seed coat color genotypes. J Agri Food Chem, 5, 7879–7883. 8. Blois MS
  • (1958): Antioxidants determination by the use of a stable free radical. Nature, 4617, 1199-1200.
  • Bravo L (1998): Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev, 56, 317-333.
  • Cardador-Martinez A, Loacra-Pina G, Oomah BD (2002): Antioxidant activity in common beans (Phaseolus vulgaris L.). J Agri Food Chem, 50, 6975–6980.
  • Chung KT, Wong TY, Wei CI., Huang YW, Lin Y (1998): Tannins and human health: a review. Crit Rev Food Sci Nutr, 38, 421-464.
  • Crozier A, Burns J, Aziz AA, Stepwart AJ., Rabiasz HS, Jenkins GI, Edwards CA, Lean MEJ (2000): Antioxidant flavonols from fruits, vegetables and beverages: measurements and bioavailability. Biol Res, 33, 79-88.
  • Gladine C, Rock E, Morand C, Buckhart D, Durand D. (2007): Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipid peroxidation. Br J Nutr 98, 691-701.
  • Heinonen M, Lehtonen PJ, Hopla A (1998): Antioxidant activity of berry and fruit wines and liquor. J Agri Food Chem, 48, 25-31.
  • Jayaprakasha GK, Singh RP, Sakariah KK (2001): Antioxidant activity of grape seed (Vitis vinifera). Food Chem, 73, 285-290.
  • Lu Y, Sun Y, Foo LY, Mcnabb WC, Molan AL (2000): Phenolic glycosides of forage legume Onobrychis viciifolia. Phytochemistry, 55, 67–75.
  • Marais JPJ, Mueuller-Harvey I, Brandt EV, Ferreira D (2000): Polyphenols, condansed tannins and other natural products in Onobrychis viciifolia (Sainfoin). J Agric Food Chem, 48, 3440–3447.
  • Prieto P, Pineda M, Aguilar M (1999):
  • Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 269, 337-341.
  • Prior RL, Cao G (2000): Antioxidant phytochemicals in fruits and vegetables. Diet and health implications. HortScience, 35, 588-592.
  • Rice-Evans CA, Miller NJ, Paganga G (1997): Antioxidant properties of phenolic compounds. Trends Plant Sci, 2, 152-159.
  • Shahidi F, Ho CT (2000): Phytochemicals and phytopharmaceuticals. American Oil Chemists Society, Champaign, IL.
  • Shahidi F, Naczk M (2004): Antioxidant properties of food phenolics. 401-403. In: Phenolics in food and nutraceuticals, Shahidi F, Naczk M (Ed)., CRC Press, Boca Raton, FL.
  • Teepica Priya Darsini D, Sasikumar JM, Kulandhaivel M (2009): In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn. Ethnobot Leaf, 13, 263-268.
  • Tsuda T, Shiga K, Ohshima K, Kawakishi S, Osawa T (1996): Inhibition of peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L. Biochem Pharmacol, 52, 1033– 1039.
  • Urquiaga I, Leighton F (2000): Plant polyphenol antioxidants and oxidative stress. Biol Res, 33, 55-64.
  • Xu BJ, Chang SKC (2007): A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci, 72, 159-166.
  • Zahin M, Aqil F, Ahmad I. (1999). The in vitro antioxidant activity and total phenolic content of four indian medicınal plants. Int J Pharm Pharm Sci, 1, 88-95.

Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts

Yıl 2012, Cilt: 59 Sayı: 1, 23 - 27, 01.03.2012

Sinan INCE Husamettin EKICI Begum YURDAKOK

https://doi.org/10.1501/Vetfak_0000002496

Öz

In this study, in vitro antioxidant effects of the acetone, methanol, ethylacetate and water extracts of the aerial parts of sainfoin (Onobrychis viciifolia) were investigated. The phenolic content of the extracts were determined by Folin-ciocalteu method and determination of antioxidant activity were carried out by phosphomolybdenum method, β-carotene-linoleate model system, radical scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power based on the reduction of Fe+3/K3Fe(CN)6 complex to the ferrous state. The study suggested that sainfoin could be pharmaceutically exploited in future researches for its antioxidant properties where acetone and methanol extracts exhibited high antioxidant activity

Anahtar Kelimeler

Antioxidant activity, free radical scavenging activity, O. viciifolia

Kaynakça

  • Abdille MdH, Singh RP, Jayaprakasha GK, Jena BS (2005): Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem, 90, 891-896.
  • Afolayan AJ, Jimoh FO, Sofidiya MO, Koduru S, Lewu FB (2007): Medicinal potential of the root of Arctotis arctotoides. Pharm Biol, 45, 486-493.
  • Amarowicz R, Piskula M, Honke J, Rudnicka B, Troszynska A, Kozlowska H (1995): Extraction of phenolic compounds from lentil seeds (Lens culinaris) with various solvents. Pol J Food Nutr Sci, 4, 53-62.
  • Amarowicz R, Żegarska Z, Pegg RB, Karamać M, Kosińska A (2006): Antioxidant and radical scavenging activities of a barley crude extract and its fractions. Czech J Food Sci, 25, 73–80.
  • Barrau E, Fabre N, Fouraste I, Hoste H (2005): Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. J Parasitol, 131, 1–8.
  • Bartolome B, Nunez V, Monagas M, Gomez-Cordoves C (2004): In vitro antioxidant activity of red grape skins. Eur Food Res Technol, 218, 173-177.
  • Beninger CW, Hosfield GL (2003): Antioxidant activity extracts, condensed tannin fractions and pure flavonoids from Phaseolus vulgaris L. Seed coat color genotypes. J Agri Food Chem, 5, 7879–7883. 8. Blois MS
  • (1958): Antioxidants determination by the use of a stable free radical. Nature, 4617, 1199-1200.
  • Bravo L (1998): Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev, 56, 317-333.
  • Cardador-Martinez A, Loacra-Pina G, Oomah BD (2002): Antioxidant activity in common beans (Phaseolus vulgaris L.). J Agri Food Chem, 50, 6975–6980.
  • Chung KT, Wong TY, Wei CI., Huang YW, Lin Y (1998): Tannins and human health: a review. Crit Rev Food Sci Nutr, 38, 421-464.
  • Crozier A, Burns J, Aziz AA, Stepwart AJ., Rabiasz HS, Jenkins GI, Edwards CA, Lean MEJ (2000): Antioxidant flavonols from fruits, vegetables and beverages: measurements and bioavailability. Biol Res, 33, 79-88.
  • Gladine C, Rock E, Morand C, Buckhart D, Durand D. (2007): Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipid peroxidation. Br J Nutr 98, 691-701.
  • Heinonen M, Lehtonen PJ, Hopla A (1998): Antioxidant activity of berry and fruit wines and liquor. J Agri Food Chem, 48, 25-31.
  • Jayaprakasha GK, Singh RP, Sakariah KK (2001): Antioxidant activity of grape seed (Vitis vinifera). Food Chem, 73, 285-290.
  • Lu Y, Sun Y, Foo LY, Mcnabb WC, Molan AL (2000): Phenolic glycosides of forage legume Onobrychis viciifolia. Phytochemistry, 55, 67–75.
  • Marais JPJ, Mueuller-Harvey I, Brandt EV, Ferreira D (2000): Polyphenols, condansed tannins and other natural products in Onobrychis viciifolia (Sainfoin). J Agric Food Chem, 48, 3440–3447.
  • Prieto P, Pineda M, Aguilar M (1999):
  • Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 269, 337-341.
  • Prior RL, Cao G (2000): Antioxidant phytochemicals in fruits and vegetables. Diet and health implications. HortScience, 35, 588-592.
  • Rice-Evans CA, Miller NJ, Paganga G (1997): Antioxidant properties of phenolic compounds. Trends Plant Sci, 2, 152-159.
  • Shahidi F, Ho CT (2000): Phytochemicals and phytopharmaceuticals. American Oil Chemists Society, Champaign, IL.
  • Shahidi F, Naczk M (2004): Antioxidant properties of food phenolics. 401-403. In: Phenolics in food and nutraceuticals, Shahidi F, Naczk M (Ed)., CRC Press, Boca Raton, FL.
  • Teepica Priya Darsini D, Sasikumar JM, Kulandhaivel M (2009): In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn. Ethnobot Leaf, 13, 263-268.
  • Tsuda T, Shiga K, Ohshima K, Kawakishi S, Osawa T (1996): Inhibition of peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L. Biochem Pharmacol, 52, 1033– 1039.
  • Urquiaga I, Leighton F (2000): Plant polyphenol antioxidants and oxidative stress. Biol Res, 33, 55-64.
  • Xu BJ, Chang SKC (2007): A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci, 72, 159-166.
  • Zahin M, Aqil F, Ahmad I. (1999). The in vitro antioxidant activity and total phenolic content of four indian medicınal plants. Int J Pharm Pharm Sci, 1, 88-95.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA22YY85EZ
Bölüm Araştırma Makalesi
Yazarlar

Sinan INCE

Husamettin EKICI

Begum YURDAKOK

Yayımlanma Tarihi 1 Mart 2012
Yayınlandığı Sayı Yıl 2012Cilt: 59 Sayı: 1

Kaynak Göster

Bibtex @editöre mektup { auvfd555867, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2012}, volume = {59}, number = {1}, pages = {23 - 27}, doi = {10.1501/Vetfak\_0000002496}, title = {Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts}, key = {cite}, author = {Ince, Sinan and Ekıcı, Husamettin and Yurdakok, Begum} }
APA Ince, S. , Ekıcı, H. & Yurdakok, B. (2012). Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 59 (1) , 23-27 . DOI: 10.1501/Vetfak_0000002496
MLA Ince, S. , Ekıcı, H. , Yurdakok, B. "Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 (2012 ): 23-27 <http://vetjournal.ankara.edu.tr/tr/pub/issue/44728/555867>
Chicago Ince, S. , Ekıcı, H. , Yurdakok, B. "Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts". Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 (2012 ): 23-27
RIS TY - JOUR T1 - Korunga (Onobrychis Viciifolia) bitkisinin antioksidan etkinliğinin in vitro değerlendirilmesi AU - SinanInce, HusamettinEkıcı, BegumYurdakok Y1 - 2012 PY - 2012 N1 - doi: 10.1501/Vetfak_0000002496 DO - 10.1501/Vetfak_0000002496 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 23 EP - 27 VL - 59 IS - 1 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002496 UR - https://doi.org/10.1501/Vetfak_0000002496 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts %A Sinan Ince , Husamettin Ekıcı , Begum Yurdakok %T Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts %D 2012 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 59 %N 1 %R doi: 10.1501/Vetfak_0000002496 %U 10.1501/Vetfak_0000002496
ISNAD Ince, Sinan , Ekıcı, Husamettin , Yurdakok, Begum . "Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts". Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 / 1 (Mart 2012): 23-27 . https://doi.org/10.1501/Vetfak_0000002496
AMA Ince S. , Ekıcı H. , Yurdakok B. Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts. Ankara Univ Vet Fak Derg. 2012; 59(1): 23-27.
Vancouver Ince S. , Ekıcı H. , Yurdakok B. Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2012; 59(1): 23-27.
IEEE S. Ince , H. Ekıcı ve B. Yurdakok , "Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 59, sayı. 1, ss. 23-27, Mar. 2012, doi:10.1501/Vetfak_0000002496