Öz
Bu çalışmada, korunga bitkisinin (Onobrychis viciifolia) toprak üstü kısımlarının aseton, methanol, etilasetat ve su ekstrelerinin antioksidan etkinliği araştırıldı. Ekstrelerin fenolik içeriği Folin-Ciocalteu yöntemiyle, antioksidan aktivitesinin belirlenmesi fosfomolibden metodu, beta-karoten-linoleat model sistemi, radikal süpürücü etkiye karşı 2,2-difenil-1-pikrilhidrazil (DPPH) ve demirli kompleks olan Fe+3/K3Fe(CN)6 bileşiğinin indirgenmesi ile gerçekleştirildi. Bu çalışma, yüksek antioksidan özelliği gösteren korunga bitkisinin aseton ve metanollü ekstrelerinin, ileride farmasötik çalışmalar için değerlendirilebileceğini önermektedir
Anahtar Kelimeler
Antioksidan aktivite serbest radikal süpürücü aktivite O. Viciifolia
Kaynakça
- Abdille MdH, Singh RP, Jayaprakasha GK, Jena BS (2005): Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem, 90, 891-896.
- Afolayan AJ, Jimoh FO, Sofidiya MO, Koduru S, Lewu FB (2007): Medicinal potential of the root of Arctotis arctotoides. Pharm Biol, 45, 486-493.
- Amarowicz R, Piskula M, Honke J, Rudnicka B, Troszynska A, Kozlowska H (1995): Extraction of phenolic compounds from lentil seeds (Lens culinaris) with various solvents. Pol J Food Nutr Sci, 4, 53-62.
- Amarowicz R, Żegarska Z, Pegg RB, Karamać M, Kosińska A (2006): Antioxidant and radical scavenging activities of a barley crude extract and its fractions. Czech J Food Sci, 25, 73–80.
- Barrau E, Fabre N, Fouraste I, Hoste H (2005): Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. J Parasitol, 131, 1–8.
- Bartolome B, Nunez V, Monagas M, Gomez-Cordoves C (2004): In vitro antioxidant activity of red grape skins. Eur Food Res Technol, 218, 173-177.
- Beninger CW, Hosfield GL (2003): Antioxidant activity extracts, condensed tannin fractions and pure flavonoids from Phaseolus vulgaris L. Seed coat color genotypes. J Agri Food Chem, 5, 7879–7883. 8. Blois MS
- (1958): Antioxidants determination by the use of a stable free radical. Nature, 4617, 1199-1200.
- Bravo L (1998): Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev, 56, 317-333.
- Cardador-Martinez A, Loacra-Pina G, Oomah BD (2002): Antioxidant activity in common beans (Phaseolus vulgaris L.). J Agri Food Chem, 50, 6975–6980.
- Chung KT, Wong TY, Wei CI., Huang YW, Lin Y (1998): Tannins and human health: a review. Crit Rev Food Sci Nutr, 38, 421-464.
- Crozier A, Burns J, Aziz AA, Stepwart AJ., Rabiasz HS, Jenkins GI, Edwards CA, Lean MEJ (2000): Antioxidant flavonols from fruits, vegetables and beverages: measurements and bioavailability. Biol Res, 33, 79-88.
- Gladine C, Rock E, Morand C, Buckhart D, Durand D. (2007): Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipid peroxidation. Br J Nutr 98, 691-701.
- Heinonen M, Lehtonen PJ, Hopla A (1998): Antioxidant activity of berry and fruit wines and liquor. J Agri Food Chem, 48, 25-31.
- Jayaprakasha GK, Singh RP, Sakariah KK (2001): Antioxidant activity of grape seed (Vitis vinifera). Food Chem, 73, 285-290.
- Lu Y, Sun Y, Foo LY, Mcnabb WC, Molan AL (2000): Phenolic glycosides of forage legume Onobrychis viciifolia. Phytochemistry, 55, 67–75.
- Marais JPJ, Mueuller-Harvey I, Brandt EV, Ferreira D (2000): Polyphenols, condansed tannins and other natural products in Onobrychis viciifolia (Sainfoin). J Agric Food Chem, 48, 3440–3447.
- Prieto P, Pineda M, Aguilar M (1999):
- Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 269, 337-341.
- Prior RL, Cao G (2000): Antioxidant phytochemicals in fruits and vegetables. Diet and health implications. HortScience, 35, 588-592.
- Rice-Evans CA, Miller NJ, Paganga G (1997): Antioxidant properties of phenolic compounds. Trends Plant Sci, 2, 152-159.
- Shahidi F, Ho CT (2000): Phytochemicals and phytopharmaceuticals. American Oil Chemists Society, Champaign, IL.
- Shahidi F, Naczk M (2004): Antioxidant properties of food phenolics. 401-403. In: Phenolics in food and nutraceuticals, Shahidi F, Naczk M (Ed)., CRC Press, Boca Raton, FL.
- Teepica Priya Darsini D, Sasikumar JM, Kulandhaivel M (2009): In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn. Ethnobot Leaf, 13, 263-268.
- Tsuda T, Shiga K, Ohshima K, Kawakishi S, Osawa T (1996): Inhibition of peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L. Biochem Pharmacol, 52, 1033– 1039.
- Urquiaga I, Leighton F (2000): Plant polyphenol antioxidants and oxidative stress. Biol Res, 33, 55-64.
- Xu BJ, Chang SKC (2007): A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci, 72, 159-166.
- Zahin M, Aqil F, Ahmad I. (1999). The in vitro antioxidant activity and total phenolic content of four indian medicınal plants. Int J Pharm Pharm Sci, 1, 88-95.
Öz
In this study, in vitro antioxidant effects of the acetone, methanol, ethylacetate and water extracts of the aerial parts of sainfoin (Onobrychis viciifolia) were investigated. The phenolic content of the extracts were determined by Folin-ciocalteu method and determination of antioxidant activity were carried out by phosphomolybdenum method, β-carotene-linoleate model system, radical scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power based on the reduction of Fe+3/K3Fe(CN)6 complex to the ferrous state. The study suggested that sainfoin could be pharmaceutically exploited in future researches for its antioxidant properties where acetone and methanol extracts exhibited high antioxidant activity
Anahtar Kelimeler
Antioxidant activity free radical scavenging activity O. viciifolia
Kaynakça
- Abdille MdH, Singh RP, Jayaprakasha GK, Jena BS (2005): Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem, 90, 891-896.
- Afolayan AJ, Jimoh FO, Sofidiya MO, Koduru S, Lewu FB (2007): Medicinal potential of the root of Arctotis arctotoides. Pharm Biol, 45, 486-493.
- Amarowicz R, Piskula M, Honke J, Rudnicka B, Troszynska A, Kozlowska H (1995): Extraction of phenolic compounds from lentil seeds (Lens culinaris) with various solvents. Pol J Food Nutr Sci, 4, 53-62.
- Amarowicz R, Żegarska Z, Pegg RB, Karamać M, Kosińska A (2006): Antioxidant and radical scavenging activities of a barley crude extract and its fractions. Czech J Food Sci, 25, 73–80.
- Barrau E, Fabre N, Fouraste I, Hoste H (2005): Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. J Parasitol, 131, 1–8.
- Bartolome B, Nunez V, Monagas M, Gomez-Cordoves C (2004): In vitro antioxidant activity of red grape skins. Eur Food Res Technol, 218, 173-177.
- Beninger CW, Hosfield GL (2003): Antioxidant activity extracts, condensed tannin fractions and pure flavonoids from Phaseolus vulgaris L. Seed coat color genotypes. J Agri Food Chem, 5, 7879–7883. 8. Blois MS
- (1958): Antioxidants determination by the use of a stable free radical. Nature, 4617, 1199-1200.
- Bravo L (1998): Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev, 56, 317-333.
- Cardador-Martinez A, Loacra-Pina G, Oomah BD (2002): Antioxidant activity in common beans (Phaseolus vulgaris L.). J Agri Food Chem, 50, 6975–6980.
- Chung KT, Wong TY, Wei CI., Huang YW, Lin Y (1998): Tannins and human health: a review. Crit Rev Food Sci Nutr, 38, 421-464.
- Crozier A, Burns J, Aziz AA, Stepwart AJ., Rabiasz HS, Jenkins GI, Edwards CA, Lean MEJ (2000): Antioxidant flavonols from fruits, vegetables and beverages: measurements and bioavailability. Biol Res, 33, 79-88.
- Gladine C, Rock E, Morand C, Buckhart D, Durand D. (2007): Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipid peroxidation. Br J Nutr 98, 691-701.
- Heinonen M, Lehtonen PJ, Hopla A (1998): Antioxidant activity of berry and fruit wines and liquor. J Agri Food Chem, 48, 25-31.
- Jayaprakasha GK, Singh RP, Sakariah KK (2001): Antioxidant activity of grape seed (Vitis vinifera). Food Chem, 73, 285-290.
- Lu Y, Sun Y, Foo LY, Mcnabb WC, Molan AL (2000): Phenolic glycosides of forage legume Onobrychis viciifolia. Phytochemistry, 55, 67–75.
- Marais JPJ, Mueuller-Harvey I, Brandt EV, Ferreira D (2000): Polyphenols, condansed tannins and other natural products in Onobrychis viciifolia (Sainfoin). J Agric Food Chem, 48, 3440–3447.
- Prieto P, Pineda M, Aguilar M (1999):
- Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 269, 337-341.
- Prior RL, Cao G (2000): Antioxidant phytochemicals in fruits and vegetables. Diet and health implications. HortScience, 35, 588-592.
- Rice-Evans CA, Miller NJ, Paganga G (1997): Antioxidant properties of phenolic compounds. Trends Plant Sci, 2, 152-159.
- Shahidi F, Ho CT (2000): Phytochemicals and phytopharmaceuticals. American Oil Chemists Society, Champaign, IL.
- Shahidi F, Naczk M (2004): Antioxidant properties of food phenolics. 401-403. In: Phenolics in food and nutraceuticals, Shahidi F, Naczk M (Ed)., CRC Press, Boca Raton, FL.
- Teepica Priya Darsini D, Sasikumar JM, Kulandhaivel M (2009): In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn. Ethnobot Leaf, 13, 263-268.
- Tsuda T, Shiga K, Ohshima K, Kawakishi S, Osawa T (1996): Inhibition of peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L. Biochem Pharmacol, 52, 1033– 1039.
- Urquiaga I, Leighton F (2000): Plant polyphenol antioxidants and oxidative stress. Biol Res, 33, 55-64.
- Xu BJ, Chang SKC (2007): A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci, 72, 159-166.
- Zahin M, Aqil F, Ahmad I. (1999). The in vitro antioxidant activity and total phenolic content of four indian medicınal plants. Int J Pharm Pharm Sci, 1, 88-95.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Diğer ID | JA22YY85EZ |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Mart 2012 |
| Yayımlandığı Sayı | Yıl 2012Cilt: 59 Sayı: 1 |