Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium

Yıl 2012, Cilt: 59 Sayı: 1, 65 - 70, 01.03.2012

Deniz YÜKSEL Nefise AKÇELİK Mustafa AKÇELİK

https://doi.org/10.1501/Vetfak_0000002503

Öz

The stj fimbrial operon harbors five genes, stjABCDE. The operon plays role in the persistence and virulence of S. Typhimurium in host systems. In this study, in vitro activation of the stj operon was achieved by transposon (TPOP) mutagenesis. The transposon insertion site was located within the cadC gene promoter in the stj operon-activated mutant strain MDN20. Comparative analysis of two-dimensional gel electrophoresis protein patterns of the wild type strain (S. Typhimurium LT2) and mutant strain MDN20 revealed that the CadC and StjB proteins were produced by mutant strain MDN20 but not by the wild type strain. These results were also confirmed by Maldi-TOF analysis. These findings indicate that cadC gene is a transcriptional activator of stj fimbrial operon in S. Typhimurium

Anahtar Kelimeler

S. Typhimurium, stj operon, cadC, transcriptional activator

Kaynakça

  • Akkoç N, Özden B, Tan BG, Akçelik M (2009): The role of stj fimbriyal operone in the intestinal persistence of Salmonella Typhimurium in mice. Biologia, 5, 859-863.
  • Bradford MM (1976): A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Boichem, 72: 248-254.
  • Chessa D, Winter MG, Nuccio SP, Tükel Ç, Baumler AJ (2008): RossE repress std fimbrial expression in Salmonella enterica serotype Typhimurium. Mol Microbiol, 68, 573–587.
  • Foster JW (2000): Microbial responses to acid stres, In G. Storzand R. engge-Aronis (ed.), Bacterial stress responses. ASM Press, Washington, DC.
  • Foster JW, Spector M (1995): How Salmonella survives against the odds. Annu Rev Microbiol, 49, 145–174.
  • Garrels M (1979): Two-dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. J Biol Chem, 254, 7961-7977.
  • Jakomin M, Chessa D, Baumler AJ, Casadesus J (2008): Regulation of the Salmonella enterica std fimbrial operon by DNA Adenine Methylation, SeqA, and HdfR. J Bacteriol 190, 7406-7413.
  • Kuper C, Jung K (2005): CadC-mediated activation of the cadBA promoter in Escherichia coli. J Mol Microbiol Biotechnol, 10, 26–39.
  • Lee YH, Kim BH, Kim JH, Yoon WS, Bang SH, Park YK (2007): CadC Has a Global Translational Effect during Acid Adaptation in Salmonella enterica Serovar Typhimurium. J Bacteriol, 189, 2417-2425.
  • McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK (2001): Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature, 413, 852–856.
  • Meng SY, Bennett GN (1992): Nucleotide sequence of the Escherichiacoli cad operon: a system for neutralization of low extracellular pH. J Bacteriol, 174, 2659–2669.
  • Merrell DS, Camilli A (2000): Regulation of Vibrio cholerae genes required for acid tolerance by a member of the “ToxR-like” family of transcriptional regulators. J Bacteriol, 182, 5342–5350.
  • Miller JH (1972): Expriments in Molecular Genetics. Cold Laboratory Press, Cold Spring Harbor, New York, USA.
  • Park YK, Bearson B, Ban, SH, Bang IS, Foster JW (1996): Internal pH crisis, lysine decarboxylase and the acid tolerance response of Salmonella typhimurium. Mol Microbiol, 20, 605–611.
  • Rappleye CA, Roth JR (1997): A Tn10 derivate (T-POP) for isolation of insertions with conditional (tetracycline- dependent) phenotypes. J Bacteriol, 179, 5827-5834.
  • Rhee JE, Kim KS, Choi SH (2005): CadC activates pH- dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. J Bacteriol, 187, 7870–7875.
  • Schmieger h (1972): Phage p22 mutants with increased or decreased transduction activities. Mol Genet Genom, 119, 75-88.
  • Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M (2007): In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocoles, 1, 2856-2860.
  • Soksawatmaekhin W, Kuraishi A, Sakata K, Kashiwagi K, Igarashi K (2004): Excretion and uptake of cadaverine by CadB and its physiological functions in Escherichia coli. Mol Microbiol, 51, 1401–1412.
  • Soksawatmaekhin W, Uemura T, Fukiwake N, Kashiwagi K, Igarashi K (2006): Identification of the cadaverine recognition site on the cadaverine-lysine antiporter CadB. J Biol Chem, 281, 29213–29220.
  • Tükel Ç, Akçelik M, de Jong MF, Şimşek Ö, Tsolis RM, Baumler AJ (2007): MarT activates expression of the MisL autotransporter protein of Salmonella enterica Serotype Typhimurium. J Bacteriol, 189, 3922–3926.
  • Watson N, Dunyak DS, Rosey EL, Slonczewski JL, Olson ER (1992): Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH. J Bacteriol, 174, 530–540.
  • Weening EH, Barker JD, Laarakker MC, Humphries AD, Tsolis RM, Baumler AJ (2005): The Salmonella enterica serotype Typhimurium lpf, bcf, stb, stc, std, and sth fimbrial operons are required for intestinal persistence in mice. Infect Immun, 73, 3358–3366.
  • Zhao B, Houry WA (2010): Acid stress response in enteropathogenic gammaproteobacteria: an aptitude for survival. Biochem Cell Biol, 88, 301-314.

Salmonella Typhimurium’da stj fimbriyal operonunun transkripsiyonel regülasyonu.

Yıl 2012, Cilt: 59 Sayı: 1, 65 - 70, 01.03.2012

Deniz YÜKSEL Nefise AKÇELİK Mustafa AKÇELİK

https://doi.org/10.1501/Vetfak_0000002503

Öz

Salmonella Typhimurium’da konakçı sistemde kalıcılığı ve virulensliğinde rol oynayan stj fimbriyal operonu, stjABCDE olmak üzere beş gen içermektedir. Bu çalışmada, stj fimbriyal operonunun in vitro koşullarda aktivasyonu transpozon (TPOP) mutasyonu yolu ile gerçekleştirildi. stj operonu aktive edilen mutant suşta (MDN20), traspozonun cadC geni promotoruna girdiği belirlendi. Doğal suş (S. Typhimurium LT2) ve MDN20 mutant suşunda gerçekleştirilen iki yönlü jel elektroforezi çalışmaları sonucunda; CadC ve StjB proteinlerinin doğal suşta üretilmediği, ancak MDN20 mutant suşunda üretildiği saptandı. Bu sonuçlar ayrıca Maldi-TOF analizleri ile de doğrulandı. Bu bulgular, S. Typhimurium’da cadC geninin, stj operonunun transkripsiyonel aktivatörü olduğunu gösterdi

Anahtar Kelimeler

S. Typhimurium, stj operonu, cadC, transkripsiyonel aktivatör

Kaynakça

  • Akkoç N, Özden B, Tan BG, Akçelik M (2009): The role of stj fimbriyal operone in the intestinal persistence of Salmonella Typhimurium in mice. Biologia, 5, 859-863.
  • Bradford MM (1976): A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Boichem, 72: 248-254.
  • Chessa D, Winter MG, Nuccio SP, Tükel Ç, Baumler AJ (2008): RossE repress std fimbrial expression in Salmonella enterica serotype Typhimurium. Mol Microbiol, 68, 573–587.
  • Foster JW (2000): Microbial responses to acid stres, In G. Storzand R. engge-Aronis (ed.), Bacterial stress responses. ASM Press, Washington, DC.
  • Foster JW, Spector M (1995): How Salmonella survives against the odds. Annu Rev Microbiol, 49, 145–174.
  • Garrels M (1979): Two-dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. J Biol Chem, 254, 7961-7977.
  • Jakomin M, Chessa D, Baumler AJ, Casadesus J (2008): Regulation of the Salmonella enterica std fimbrial operon by DNA Adenine Methylation, SeqA, and HdfR. J Bacteriol 190, 7406-7413.
  • Kuper C, Jung K (2005): CadC-mediated activation of the cadBA promoter in Escherichia coli. J Mol Microbiol Biotechnol, 10, 26–39.
  • Lee YH, Kim BH, Kim JH, Yoon WS, Bang SH, Park YK (2007): CadC Has a Global Translational Effect during Acid Adaptation in Salmonella enterica Serovar Typhimurium. J Bacteriol, 189, 2417-2425.
  • McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK (2001): Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature, 413, 852–856.
  • Meng SY, Bennett GN (1992): Nucleotide sequence of the Escherichiacoli cad operon: a system for neutralization of low extracellular pH. J Bacteriol, 174, 2659–2669.
  • Merrell DS, Camilli A (2000): Regulation of Vibrio cholerae genes required for acid tolerance by a member of the “ToxR-like” family of transcriptional regulators. J Bacteriol, 182, 5342–5350.
  • Miller JH (1972): Expriments in Molecular Genetics. Cold Laboratory Press, Cold Spring Harbor, New York, USA.
  • Park YK, Bearson B, Ban, SH, Bang IS, Foster JW (1996): Internal pH crisis, lysine decarboxylase and the acid tolerance response of Salmonella typhimurium. Mol Microbiol, 20, 605–611.
  • Rappleye CA, Roth JR (1997): A Tn10 derivate (T-POP) for isolation of insertions with conditional (tetracycline- dependent) phenotypes. J Bacteriol, 179, 5827-5834.
  • Rhee JE, Kim KS, Choi SH (2005): CadC activates pH- dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. J Bacteriol, 187, 7870–7875.
  • Schmieger h (1972): Phage p22 mutants with increased or decreased transduction activities. Mol Genet Genom, 119, 75-88.
  • Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M (2007): In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocoles, 1, 2856-2860.
  • Soksawatmaekhin W, Kuraishi A, Sakata K, Kashiwagi K, Igarashi K (2004): Excretion and uptake of cadaverine by CadB and its physiological functions in Escherichia coli. Mol Microbiol, 51, 1401–1412.
  • Soksawatmaekhin W, Uemura T, Fukiwake N, Kashiwagi K, Igarashi K (2006): Identification of the cadaverine recognition site on the cadaverine-lysine antiporter CadB. J Biol Chem, 281, 29213–29220.
  • Tükel Ç, Akçelik M, de Jong MF, Şimşek Ö, Tsolis RM, Baumler AJ (2007): MarT activates expression of the MisL autotransporter protein of Salmonella enterica Serotype Typhimurium. J Bacteriol, 189, 3922–3926.
  • Watson N, Dunyak DS, Rosey EL, Slonczewski JL, Olson ER (1992): Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH. J Bacteriol, 174, 530–540.
  • Weening EH, Barker JD, Laarakker MC, Humphries AD, Tsolis RM, Baumler AJ (2005): The Salmonella enterica serotype Typhimurium lpf, bcf, stb, stc, std, and sth fimbrial operons are required for intestinal persistence in mice. Infect Immun, 73, 3358–3366.
  • Zhao B, Houry WA (2010): Acid stress response in enteropathogenic gammaproteobacteria: an aptitude for survival. Biochem Cell Biol, 88, 301-314.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Diğer ID JA88SM93JA
Bölüm Araştırma Makalesi
Yazarlar

Deniz YÜKSEL

Nefise AKÇELİK

Mustafa AKÇELİK

Yayımlanma Tarihi 1 Mart 2012
Yayınlandığı Sayı Yıl 2012Cilt: 59 Sayı: 1

Kaynak Göster

Bibtex @editöre mektup { auvfd555883, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2012}, volume = {59}, number = {1}, pages = {65 - 70}, doi = {10.1501/Vetfak\_0000002503}, title = {Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium}, key = {cite}, author = {Yüksel, Deniz and Akçelik, Nefise and Akçelik, Mustafa} }
APA Yüksel, D. , Akçelik, N. & Akçelik, M. (2012). Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 59 (1) , 65-70 . DOI: 10.1501/Vetfak_0000002503
MLA Yüksel, D. , Akçelik, N. , Akçelik, M. "Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 (2012 ): 65-70 <http://vetjournal.ankara.edu.tr/tr/pub/issue/44728/555883>
Chicago Yüksel, D. , Akçelik, N. , Akçelik, M. "Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium". Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 (2012 ): 65-70
RIS TY - JOUR T1 - Salmonella Typhimurium’da stj fimbriyal operonunun transkripsiyonel regülasyonu. AU - DenizYüksel, NefiseAkçelik, MustafaAkçelik Y1 - 2012 PY - 2012 N1 - doi: 10.1501/Vetfak_0000002503 DO - 10.1501/Vetfak_0000002503 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 65 EP - 70 VL - 59 IS - 1 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002503 UR - https://doi.org/10.1501/Vetfak_0000002503 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium %A Deniz Yüksel , Nefise Akçelik , Mustafa Akçelik %T Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium %D 2012 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 59 %N 1 %R doi: 10.1501/Vetfak_0000002503 %U 10.1501/Vetfak_0000002503
ISNAD Yüksel, Deniz , Akçelik, Nefise , Akçelik, Mustafa . "Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium". Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 / 1 (Mart 2012): 65-70 . https://doi.org/10.1501/Vetfak_0000002503
AMA Yüksel D. , Akçelik N. , Akçelik M. Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium. Ankara Univ Vet Fak Derg. 2012; 59(1): 65-70.
Vancouver Yüksel D. , Akçelik N. , Akçelik M. Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2012; 59(1): 65-70.
IEEE D. Yüksel , N. Akçelik ve M. Akçelik , "Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 59, sayı. 1, ss. 65-70, Mar. 2012, doi:10.1501/Vetfak_0000002503