Hereditary diseases cause yield and economic loses. It is important to examine hereditary diseases at the molecular level and to remove diseases from the herd. In our study, it was aimed to determine allele frequencies of genes that cause bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation diseases in Holstein cattle. Blood samples were randomly taken from 300 Holstein cattle in different dairy farms in Kocaeli, Sakarya and Balıkesir provinces. Deoxyribonucleic acid samples were isolated from blood samples by using the standard ammonium acetate salt-out method. The target regions were amplified by polymerase chain reaction to determine the mutant alleles causing bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation. According to the nucleotide chromotograms of the samples subjected to bovine leukocyte adhesion deficiency analysis, it was determined that 4 out of 300 cattle were heterozygous and 296 were homozygous. Polymerase chain reaction procedure for factor XI deficiency disease was sufficient, while samples amplified by polymerase chain reaction for complex vertebral malformation disease were subjected to restriction particle length polymorphism. Factor XI deficiency and complex vertebral malformation disease genes were all homozygous normal.
Anaemia, β2 integrin glycoprotein, Holstein, point mutation, PCR-RFLP, SLC35A3
Birincil Dil | İngilizce |
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Konular | Veteriner Cerrahi |
Bölüm | Kısa Bildiri |
Yazarlar |
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Yayımlanma Tarihi | 14 Haziran 2019 |
Yayınlandığı Sayı | Yıl 2019Cilt: 66 Sayı: 3 |
Bibtex | @kısa bildiri { auvfd436199, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2019}, volume = {66}, number = {3}, pages = {311 - 316}, doi = {10.33988/auvfd.436199}, title = {Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle}, key = {cite}, author = {Hacıhasanoğlu Çakmak, Neziha and Yardibi, Hasret} } |
APA | Hacıhasanoğlu Çakmak, N. & Yardibi, H. (2019). Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 66 (3) , 311-316 . DOI: 10.33988/auvfd.436199 |
MLA | Hacıhasanoğlu Çakmak, N. , Yardibi, H. "Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 311-316 <http://vetjournal.ankara.edu.tr/tr/pub/issue/45755/436199> |
Chicago | Hacıhasanoğlu Çakmak, N. , Yardibi, H. "Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 311-316 |
RIS | TY - JOUR T1 - Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle AU - NezihaHacıhasanoğlu Çakmak, HasretYardibi Y1 - 2019 PY - 2019 N1 - doi: 10.33988/auvfd.436199 DO - 10.33988/auvfd.436199 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 311 EP - 316 VL - 66 IS - 3 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.436199 UR - https://doi.org/10.33988/auvfd.436199 Y2 - 2019 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle %A Neziha Hacıhasanoğlu Çakmak , Hasret Yardibi %T Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle %D 2019 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 66 %N 3 %R doi: 10.33988/auvfd.436199 %U 10.33988/auvfd.436199 |
ISNAD | Hacıhasanoğlu Çakmak, Neziha , Yardibi, Hasret . "Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 / 3 (Haziran 2019): 311-316 . https://doi.org/10.33988/auvfd.436199 |
AMA | Hacıhasanoğlu Çakmak N. , Yardibi H. Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle. Ankara Univ Vet Fak Derg. 2019; 66(3): 311-316. |
Vancouver | Hacıhasanoğlu Çakmak N. , Yardibi H. Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2019; 66(3): 311-316. |
IEEE | N. Hacıhasanoğlu Çakmak ve H. Yardibi , "Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 66, sayı. 3, ss. 311-316, Haz. 2019, doi:10.33988/auvfd.436199 |