Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey
Yıl 2008, Cilt: 55 Sayı: 2, 103 - 106, 01.09.2008Öz
In this study, the isolation of C. sordellii from abomasum lesions of lambs and the confirmation of the isolates by polymerase chain reaction (PCR) was attempted. A total of 298 abomasal specimens (swabs) obtained from lambs of 4 to 8 months of age were investigated. Sampled swabs were inoculated onto blood agar plates and incubated anaerobically. Following the incubation grown colonies were identified according to their morphological and biochemical characteristics. C. sordellii was isolated from 17 of 298 specimens (5.7%). These bacteria were also confirmed to harbour C. sordellii DNA by PCR. In conclusion, the presence of C. sordellii in abomasum lesions was demonstrated in Turkey for the first time with this study
Anahtar Kelimeler
Abomasum lesions, Clostridium sordellii, isolation, lamb, PCR
Kaynakça
- Al-Mashat RR, Taylor DJ (1983a): Bacteria in enteric lesions of cattle. Vet Rec, 112, 5-10.
- Al-Mashat RR, Taylor DJ (1983b): Clostridium sordellii in enteritis in an adult sheep. Vet Rec, 112, 19.
- Green GA, Schue V, Monteil H (1995): Cloning and characterization of the cytotoxin L-encoding gene of Clostridium sordellii: homology with Clostridium difficile cytotoxin B. Gene, 161, 57-61.
- Grossman N, Ron EZ (1975): Membrane-bound DNA from Escherichia coli: extraction by freeze-thaw-lysozyme. FEBS Lett, 54, 327-329.
- Hibbs CM, Johnson DR, Reynolds K, Harrington RJR (1977): Clostridium sordellii isolated from foals. Vet Med Small Anim Clin, 72, 256-258.
- Kikuchi E, Miyamoto Y, Narushima S, Itoh K (2002): Design of species-specific primers to identify 13 species of Clostridium harbored in human intestinal tracts. Microbiol Immunol, 46, 353-358.
- Lewis CJ, Naylor R (1996): Sudden death in lambs associated with Clostridium sordellii infection. Vet Rec, 138, 262.
- Lewis CJ, Naylor R (1998): Sudden death in sheep associated with Clostridium sordellii. Vet Rec, 142, 417- 421.
- Morris WE, Uzal FA, Fattorini FR, Terzolo H (2002): Malignant oedema associated with blood-sampling in sheep. Australian Vet J, 80, 280-281.
- Richards SM, Hunt BW (1982): Clostridium sordellii in lambs. Vet Rec, 111, 22.
- Uzal FA, Hugenholtz P, Blackall LL, Petray S, Moss S, Assis RA, Miyakawa MF, Carloni G (2003): PCR detection of Clostridium chauvoei in pure cultures and formalin-fixed, paraffin-embedded tissues. Vet Microbiol, 91, 239-248.
Türkiye’deki kuzularda abomazum lezyonlarından Clostridium sordellii izolasyonu
Yıl 2008, Cilt: 55 Sayı: 2, 103 - 106, 01.09.2008Öz
Bu çalışmada kuzuların abomazum lezyonlarından Clostridium sordellii izolasyonu ve izolatların polimeraz zincir reaksiyonu ile doğrulanması amaçlandı. Çalışma kapsamında 4-8 aylık yaştaki koyunlardan toplanan 298 abomazumdan alınan svab örnekleri incelendi. Sıvabların kanlı agarlara ekimi gerçekleştirilerek anaerobik ortamda inkube edildi. İnkubasyonu takiben şekillenen koloniler morfolojik ve biyokimyasal özelliklerine göre identifiye edildi. İncelen 298 materyalin 17 (% 5.7)’sinde C. sordellii izole edildi. İzolatlar PCR ile C. sordellii DNA’sı yönünden doğrulandı. Sonuç olarak, bu çalışma ile abomazum lezyonlarından C. sordellii izolasyonu Türkiye’de ilk kez gerçekleştirildi
Anahtar Kelimeler
Abomazum lezyonları, Clostridium sordellii, izolasyon, kuzu, PCR
Kaynakça
- Al-Mashat RR, Taylor DJ (1983a): Bacteria in enteric lesions of cattle. Vet Rec, 112, 5-10.
- Al-Mashat RR, Taylor DJ (1983b): Clostridium sordellii in enteritis in an adult sheep. Vet Rec, 112, 19.
- Green GA, Schue V, Monteil H (1995): Cloning and characterization of the cytotoxin L-encoding gene of Clostridium sordellii: homology with Clostridium difficile cytotoxin B. Gene, 161, 57-61.
- Grossman N, Ron EZ (1975): Membrane-bound DNA from Escherichia coli: extraction by freeze-thaw-lysozyme. FEBS Lett, 54, 327-329.
- Hibbs CM, Johnson DR, Reynolds K, Harrington RJR (1977): Clostridium sordellii isolated from foals. Vet Med Small Anim Clin, 72, 256-258.
- Kikuchi E, Miyamoto Y, Narushima S, Itoh K (2002): Design of species-specific primers to identify 13 species of Clostridium harbored in human intestinal tracts. Microbiol Immunol, 46, 353-358.
- Lewis CJ, Naylor R (1996): Sudden death in lambs associated with Clostridium sordellii infection. Vet Rec, 138, 262.
- Lewis CJ, Naylor R (1998): Sudden death in sheep associated with Clostridium sordellii. Vet Rec, 142, 417- 421.
- Morris WE, Uzal FA, Fattorini FR, Terzolo H (2002): Malignant oedema associated with blood-sampling in sheep. Australian Vet J, 80, 280-281.
- Richards SM, Hunt BW (1982): Clostridium sordellii in lambs. Vet Rec, 111, 22.
- Uzal FA, Hugenholtz P, Blackall LL, Petray S, Moss S, Assis RA, Miyakawa MF, Carloni G (2003): PCR detection of Clostridium chauvoei in pure cultures and formalin-fixed, paraffin-embedded tissues. Vet Microbiol, 91, 239-248.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Makaleler |
| Yazarlar |
|
| Yayımlanma Tarihi | 1 Eylül 2008 |
| Yayınlandığı Sayı | Yıl 2008Cilt: 55 Sayı: 2 |
Kaynak Göster
| Bibtex | @editöre mektup { auvfd583192, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2008}, volume = {55}, number = {2}, pages = {103 - 106}, doi = {10.1501/Vetfak\_0000000305}, title = {Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey}, key = {cite}, author = {Cantekin, Zafer} } |
| APA | Akan, M. , Sareyyüpoğlu, B. , Öncel, T. , Tel, O. Y. , İlhan, Z. & Cantekin, Z. (2008). Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 55 (2) , 103-106 . DOI: 10.1501/Vetfak_0000000305 |
| MLA | Akan, M. , Sareyyüpoğlu, B. , Öncel, T. , Tel, O. Y. , İlhan, Z. , Cantekin, Z. "Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 55 (2008 ): 103-106 <http://vetjournal.ankara.edu.tr/tr/pub/issue/46417/583192> |
| Chicago | Akan, M. , Sareyyüpoğlu, B. , Öncel, T. , Tel, O. Y. , İlhan, Z. , Cantekin, Z. "Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 55 (2008 ): 103-106 |
| RIS | TY - JOUR T1 - Türkiye’deki kuzularda abomazum lezyonlarından Clostridium sordellii izolasyonu AU - MehmetAkan, BarışSareyyüpoğlu, TanerÖncel, Osman YaşarTel, Ziyaİlhan, ZaferCantekin Y1 - 2008 PY - 2008 N1 - doi: 10.1501/Vetfak_0000000305 DO - 10.1501/Vetfak_0000000305 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 103 EP - 106 VL - 55 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000000305 UR - https://doi.org/10.1501/Vetfak_0000000305 Y2 - 2023 ER - |
| EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey %A Mehmet Akan , Barış Sareyyüpoğlu , Taner Öncel , Osman Yaşar Tel , Ziya İlhan , Zafer Cantekin %T Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey %D 2008 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 55 %N 2 %R doi: 10.1501/Vetfak_0000000305 %U 10.1501/Vetfak_0000000305 |
| ISNAD | Akan, Mehmet , Sareyyüpoğlu, Barış , Öncel, Taner , Tel, Osman Yaşar , İlhan, Ziya , Cantekin, Zafer . "Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 55 / 2 (Eylül 2008): 103-106 . https://doi.org/10.1501/Vetfak_0000000305 |
| AMA | Akan M. , Sareyyüpoğlu B. , Öncel T. , Tel O. Y. , İlhan Z. , Cantekin Z. Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey. Ankara Univ Vet Fak Derg. 2008; 55(2): 103-106. |
| Vancouver | Akan M. , Sareyyüpoğlu B. , Öncel T. , Tel O. Y. , İlhan Z. , Cantekin Z. Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2008; 55(2): 103-106. |
| IEEE | M. Akan , B. Sareyyüpoğlu , T. Öncel , O. Y. Tel , Z. İlhan ve Z. Cantekin , "Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 55, sayı. 2, ss. 103-106, Eyl. 2008, doi:10.1501/Vetfak_0000000305 |