Öz
The aim of this study was to investigate the effect of different extenders on sperm motility in cryopreservation of turkey semen. Semen was collected from five toms by manual massage method twice a week for five weeks. Some spermatological parameters of collected semen was assessed and mean ejaculate volume (ml), sperm motility (%) and sperm concentration (x109/ml) were recorded as 0.28±2.8, 84.8±1.12 and 5.1±0.34 respectively. Pooled semen were divided into four equal parts and diluted 1:3 with extenders (Graham, Glucose, Ringer and Tris + Glucose) containing 10% Dimethyl sulphoxide (DMSO) and equilibrated at 4 oC for 90 min. Samples were frozen at -120 o C on liquid nitrogen vapour for 15 min. Sperm motility after dilution was averaged 74±2.6, 78±2.0, 84±2.2, 62±2.9 % and post-thaw motility was 31±2.4, 40.5±2.8, 17±2.0, 11±1.4 % respectively. According to the obtained results, glucose extender provided better environment for turkey semen undergoing cryopreservation than the others (p
Anahtar Kelimeler
Kaynakça
- Alkan S, Pabuççuoğlu S, Baran A, Özdaş B, İleri K (1998): BPSE ve PBS sulandırıcılarının hindi spermasının soğukta saklanması ve fertilite üzerine etkisi. İstanbul Üniv Vet Fak Derg, 24, 213-222.
- Akçay E, Tekin N, Selçuk M, Çevik M (1997): Hindi spermasının değişik sulandırıcılarda 4oC’de saklanması. Ankara Üniv Vet Fak Derg, 44, 137-149.
- Akçay E, Varışlı Ö, Tekin N (2006). Fertilizing ability of turkey semen diluted with simple sugar-based extenders af- ter cooled storage. XII European Poultry Conference, 11- 13 September Verona / Italy (accepted, in press).
- Bakst MR, Sexton TJ (1979): Fertilizing capacity and ultrastructure of fowl and turkey spermatozoa before and after freezing. J Reprod Fertil, 55, 1-7.
- Bakst MR, Cecil HC (1992): Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage. J Reprod Fertil, 94, 287-293.
- Blanco JM, Gee G, Wildt DE, Tselutin K and Donoghue AM (2000) :Semen cryopreservation in poultry and non-domestic species: a comparative approach to understanding the fundamentals of avian spermatozoa cryobiology. Br Poult Sci, 41, 6-7.
- Blanco JM, Gee G, Wildt DE and Donoghue AM (2000): Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle and peregrine falcon spermatozoa. Biol Reprod, 63, 1164-1171.
- Dimitrov SG, Georgiov SK and Atanasov VK (2000): A new diluent (I8.5) and method for freezing turkey semen: fertility data. Br Poult Sci, 41, 11.
- Donoghue AM. Wishart JG (2000): Storage of poultry semen. Anim Reprod Sci, 62, 213-232.
- Gill SP and Barbato GF (2006): Cryopreservation of Rooster sperm: influence of genotyypes and methodologies. Erişim:http://www.fass.org/fass01/pdfs/ Gill. pdf 03.02.2006
- Giesen AF, Sexton TJ (1983):Beltsville poultry semen extender 9. Effect of storage temperature on turkey semen held eighteen hours. Poult Sci, 62, 1305-1311.
- Graham EF, Nelson DS, Schmehl MK (1982a): Development of extender and techniques for frozen turkey semen. 1. development. Poult Sci, 61, 550-557.
- Graham EF, Nelson DS, Schmehl MK (1982b): Development of extender and techniques for frozen turkey semen. 2. fertility trials. Poult Sci, 61, 558-563.
- Graham JW and Palmer FH (1986): The effect of cryopreservation at -196o C on the viability of fowl and turkey spermatozoa assessed in vitro. Anim Reprod Sci, 10, 317-324.
- Han XF, Niu ZY, Liu FZ and Yang CS (2005): Effect of diluents, cryoprotectans, equlibration time and thawing temperature on cryopreservation of duck semen. Int J Poult Sci, 4, 197-201.
- Keskin O, Tekin N, Akçay E (1995): Erbro ırkı horoz spermalarının farklı sulandırıcı ve kryoprotektanlarla dondurulması. Lalahan Hay Arş Ens Derg, 35, 110-125.
- Laffaldano N, Meluzzi A (2003): Effect of dialysis on quality characteristics of turkey semen during liquid storage. Theriogenelogy, 60, 421-427.
- Lake PE, Stewart JM (1978): Artificial Insemination in Poultry. London Ministry of Agriculture, Fisherries and Food. Bulletin 213.
- Long JA. Kulkarni G (2004): An effective method for iproving the fettility og glycerol-exposed poultry semen. Poult Sci, 83, 1595-1601.
- Pinto O, Amir D, Schindler H, Hurwitz S (1985): Fertil- ity of fresh and stored turkey spermatozoa in the presence or absence of glucose or fructose in the suspension me- dium. Poult Sci, 64, 1388-1390.
- Sexton TJ (1988a): Comparison of commercial diluents for holding turkey semen 24 hours at 5oC. Poult Sci, 67, 131-134.
- Sexton TJ (1988b): Research not: influence of damaged spermatozoa on the fertility turkey semen stored for 24 hours at 5oC. Poult Sci, 67, 1483-1485.
- Sexton TJ (1976): Studies on the dilution of turkey semen. Br Poult Sci, 17, 179-184.
- Tekin N (1990): Erkek üreme organlarının muayenesi (Androlojik Muayeneler). 61-62. In: Erol Alaçam (Ed), Theriogenoloji. Nurol Matbacılık , Ankara.
- Tselutin K, Narubina L, Mavrodina T, Tur B (1995): Cryopreservation of poultry semen. Br Poult Sci, 36, 805-811.
- Wambeke FV and Huyghebaert G (1989): Current role of semen storage and artificial insemination in the turkey industry. Br Poult Sci, 30, 461–469.
- Wishart, GJ (1985): Quantitation of the fertilizing ability of fresh compared with frozen and thawed fowl spermatozoa. Br Poult Sci, 26, 375-380. Geliş tarihi: 03.05.2006 / Kabul tarihi: 06.06.2006
Öz
Bu çalışma, hindi spermasının dondurulmasında farklı sulandırıcıların çözüm sonrasında spermatozoa motilitesi üzerine etkisini araştırmak amacı ile yapılmıştır. Çalışmada kullanılan beş adet hindiden sperma, beş hafta süresince haftada iki kez, masaj yöntemi ile alındı. Hindilerden alınan nativ spermanın bazı spermatolojik özellikleri değerlendirildi ve ortalama ejekülat miktarı (ml), motilite (%) ve yoğunluk (x109/ml) sırasıyla 0.28±2.8, 84.8±1.12 ve 5.1±0.34 olarak belirlendi. Değerlendirmeleri yapılan sperma 4 eşit parçaya bölünerek 1:3 oranında; %10 Dimethyl sulphoxide (DMSO) içeren Graham, Glikoz, Ringer ve Tris+Glikoz sulandırıcıları ile sulandırılıp +4 oC de 90 dakika alışıma tabi tutulduktan sonra, -120 o C de sıvı azot buharında 15 dakikada donduruldu. Sulandırma sonrası ortalama motilite değerleri sırasıyla % 74±2.6, 78±2.0, 84±2.2, 62±2.9 ve çözdürme sonrasında ise % 31±2.4, 40.5±2.8, 17±2.0, 11±1.4 olarak elde edildi. Elde edilen sonuçlara göre glikoz tabanlı sulandırıcının hindi spermasının dondurularak saklanmasında diğer sulandırıcılara göre daha iyi sonuçlar verdiği saptandı (p
Anahtar Kelimeler
Hindi motilite sperma sperma sulandırıcısı spermanın dondurulması
Kaynakça
- Alkan S, Pabuççuoğlu S, Baran A, Özdaş B, İleri K (1998): BPSE ve PBS sulandırıcılarının hindi spermasının soğukta saklanması ve fertilite üzerine etkisi. İstanbul Üniv Vet Fak Derg, 24, 213-222.
- Akçay E, Tekin N, Selçuk M, Çevik M (1997): Hindi spermasının değişik sulandırıcılarda 4oC’de saklanması. Ankara Üniv Vet Fak Derg, 44, 137-149.
- Akçay E, Varışlı Ö, Tekin N (2006). Fertilizing ability of turkey semen diluted with simple sugar-based extenders af- ter cooled storage. XII European Poultry Conference, 11- 13 September Verona / Italy (accepted, in press).
- Bakst MR, Sexton TJ (1979): Fertilizing capacity and ultrastructure of fowl and turkey spermatozoa before and after freezing. J Reprod Fertil, 55, 1-7.
- Bakst MR, Cecil HC (1992): Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage. J Reprod Fertil, 94, 287-293.
- Blanco JM, Gee G, Wildt DE, Tselutin K and Donoghue AM (2000) :Semen cryopreservation in poultry and non-domestic species: a comparative approach to understanding the fundamentals of avian spermatozoa cryobiology. Br Poult Sci, 41, 6-7.
- Blanco JM, Gee G, Wildt DE and Donoghue AM (2000): Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle and peregrine falcon spermatozoa. Biol Reprod, 63, 1164-1171.
- Dimitrov SG, Georgiov SK and Atanasov VK (2000): A new diluent (I8.5) and method for freezing turkey semen: fertility data. Br Poult Sci, 41, 11.
- Donoghue AM. Wishart JG (2000): Storage of poultry semen. Anim Reprod Sci, 62, 213-232.
- Gill SP and Barbato GF (2006): Cryopreservation of Rooster sperm: influence of genotyypes and methodologies. Erişim:http://www.fass.org/fass01/pdfs/ Gill. pdf 03.02.2006
- Giesen AF, Sexton TJ (1983):Beltsville poultry semen extender 9. Effect of storage temperature on turkey semen held eighteen hours. Poult Sci, 62, 1305-1311.
- Graham EF, Nelson DS, Schmehl MK (1982a): Development of extender and techniques for frozen turkey semen. 1. development. Poult Sci, 61, 550-557.
- Graham EF, Nelson DS, Schmehl MK (1982b): Development of extender and techniques for frozen turkey semen. 2. fertility trials. Poult Sci, 61, 558-563.
- Graham JW and Palmer FH (1986): The effect of cryopreservation at -196o C on the viability of fowl and turkey spermatozoa assessed in vitro. Anim Reprod Sci, 10, 317-324.
- Han XF, Niu ZY, Liu FZ and Yang CS (2005): Effect of diluents, cryoprotectans, equlibration time and thawing temperature on cryopreservation of duck semen. Int J Poult Sci, 4, 197-201.
- Keskin O, Tekin N, Akçay E (1995): Erbro ırkı horoz spermalarının farklı sulandırıcı ve kryoprotektanlarla dondurulması. Lalahan Hay Arş Ens Derg, 35, 110-125.
- Laffaldano N, Meluzzi A (2003): Effect of dialysis on quality characteristics of turkey semen during liquid storage. Theriogenelogy, 60, 421-427.
- Lake PE, Stewart JM (1978): Artificial Insemination in Poultry. London Ministry of Agriculture, Fisherries and Food. Bulletin 213.
- Long JA. Kulkarni G (2004): An effective method for iproving the fettility og glycerol-exposed poultry semen. Poult Sci, 83, 1595-1601.
- Pinto O, Amir D, Schindler H, Hurwitz S (1985): Fertil- ity of fresh and stored turkey spermatozoa in the presence or absence of glucose or fructose in the suspension me- dium. Poult Sci, 64, 1388-1390.
- Sexton TJ (1988a): Comparison of commercial diluents for holding turkey semen 24 hours at 5oC. Poult Sci, 67, 131-134.
- Sexton TJ (1988b): Research not: influence of damaged spermatozoa on the fertility turkey semen stored for 24 hours at 5oC. Poult Sci, 67, 1483-1485.
- Sexton TJ (1976): Studies on the dilution of turkey semen. Br Poult Sci, 17, 179-184.
- Tekin N (1990): Erkek üreme organlarının muayenesi (Androlojik Muayeneler). 61-62. In: Erol Alaçam (Ed), Theriogenoloji. Nurol Matbacılık , Ankara.
- Tselutin K, Narubina L, Mavrodina T, Tur B (1995): Cryopreservation of poultry semen. Br Poult Sci, 36, 805-811.
- Wambeke FV and Huyghebaert G (1989): Current role of semen storage and artificial insemination in the turkey industry. Br Poult Sci, 30, 461–469.
- Wishart, GJ (1985): Quantitation of the fertilizing ability of fresh compared with frozen and thawed fowl spermatozoa. Br Poult Sci, 26, 375-380. Geliş tarihi: 03.05.2006 / Kabul tarihi: 06.06.2006
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Mart 2007 |
| Yayımlandığı Sayı | Yıl 2007Cilt: 54 Sayı: 1 |