Öz
Cryoprotectants used in freezing of gamete cells provide protection from cold shock and the other damages during freezing. Due to toxic effects of cryoprotectants, studies about them have been concentrated on it, and toxic effects have been tried to reduce by external cryoprotectants added. Optimum adding rates of cryoprotectants become peculiar to species and require determination of some parameters of cell membrane. Determinating of physiological and functional traits in cryopreserved-thawed cells have provided progress in cryobiological studies
Anahtar Kelimeler
Cryopreservation cryoprotectant cryoprotective effect gamet cell
Kaynakça
- 1. Alvarenga MA, Alvarenga FC, Moreıra RM, Cesarıno MM (2000): Acrozomal ultrastructure of stallion spermatozoa cryopreserved with ethylene glycol using two packing systems. Equine Vet J, 32, 541-545.
- 2. Alvarenga MA, Papa FO, Landim-alvarenga FC, Medeiros ASL (2005): Amides as cryoprotectants for freezing stallion semen: a review. Anim Reprod Sci (Basımda).
- 3. An TZ, Iwakiri M, Edashige K, Takashi M (2000): Factors affecting the survival of frozen mouse spermatozoa. Cryobiology, 40, 237-249.
- 4. Arav A, Hehu D, Mattioli M (1993): Osmotic and cytotoxic study of vitrification of ımmature bovine oocytes. J Reprod Fertil, 99, 353-358.
- 5. Ball BA, Vo A (2001): Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential. J Androl, 22, 1061-1069.
- 6. Cabria E, Anel L, Herraez MP (2001): Effect of external cryoprotectants as membrane stabilizers on cryopreserved raınbow trout sperm. Theriogenology, 52, 623-635.
- 7. Chalah T, Seigneurin F, Blesbois E, Brillard JP (1999): In vitro comparison of fowl sperm viability ın ejaculates frozen by threee different techniques and relationship with subsequent fertility in vitro. Cryobiology, 39, 185-191.
- 8. Chen Y, Foote RH, Brockett CC (1993): Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull sperm. Cryobiology, 30, 423- 431.
- 9. Cheng A, Merz KM (1997): Ice- binding mechanism of winter flounder antifreeze proteins. Biophys J, 73, 2851- 2873.
- 10. Curry MR, Watson PF (1994): Osmotic effects on ram and human sperm membranes in relation to thawing injury. Cryobiology, 31, 39-46.
- 11. Dinnyes A, Urbanyi B, Baranyai B, Magyary I (1998): Chilling sensitivity of carp (cyprinus carpio) embryos at different developmental stages in the presence or absence of cryoprotectants: work in progress. Theriogenology, 50, 1-13.
- 12. Dobrinsky JR (2002): Advancements in cryopreservation of domestic animal embryos. Theriogenology, 57, 285-302.
- 13. Donoghue AM, Wishart GJ (2000): Storage of poultry semen. Anim Reprod Sci, 62, 213-232.
- 14. Edashige K, Yamaji Y, Kleinhans FW, Kasai M (2003): Artificial expression of aquaporin-3 ımproves the survival of mouse oocytes after cryopreservation. Biol Reprod, 68, 87-94.
- 15. Gao DY, Ashworth E, Watson PF, Kleınhans FW, Mazur P, Crıster, JK (1993): Hyperosmotic tolerance of human spermatozoa: separate effects of glycerol, sodium chloride, and sucrose on spermolysis. Biol Reprod, 49, 112-23.
- 16. Gomes GM, Jacop JCF, Medeiros ASL, Papa FO, Alvarenga MA (2002): Improvement of stallion spermatozoa preservation with alternative cryoprotectants for the mangalarga marchador breed. Theriogenology, 58, 277-279.
- 17. Holt WT (2000): Fundemental aspects of sperm cryobiology: theiımportance of species and individual differences. Theriogenology, 53, 47-58.
- 18. Holt, WT (2000): Basic aspects of frozen storage of semen. Anim Reprod Sci, 62,3-22.
- 19. Kasai M (1996): Simple and efficient methods for vitrification of mammalian embryos. Anim Reprod Sci, 42, 67-75.
- 20. Kasai M (2002): Advaces in the cryopreservation of mammalian oocytes and embryos: development of ultrarapid vitrification. Reprod Med Biol, 1, 1-9.
- 21. Katkov II, Katkova N, Crister JK, Mazur P (1998): Mouse spermatozoa in high concentrations of glycerol: chemical toxicity vs osmotic shock at normal and reduced oxygen concentrations. Cryobiology, 37, 325-338.
- 22. Leeuw FED, Leeuw AMD, Daas JHG, Colenbrander BV, Erkleij AJ (1993): Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology, 30, 32-44.
Öz
Gamet hücrelerinin dondurulmasında kullanılan koruyucu maddeler (kryoprotektanlar) soğuk şoku ve donma esnasında gelişen diğer hasarlara karşı koruma sağlarlar. Kryoprotektanların hücrede toksik etkiye sahip olmaları, bu maddeler üzerindeki çalışmaları yoğunlaştırmış, ortama katılan eksternal kryoprotektanlarla, toksik etkimeler azaltılmaya çalışılmıştır. Kryoprotektanların optimum katım oranları türe özgü olmakta ve bazı hücre membran parametrelerinin bilinmesini gerektirmektedir. Dondurulançözdürülen hücrelerin fizyolojik ve fonksiyonel özelliklerinin anlaşılması ile kryobiyolojik çalışmalarda gelişme sağlanmıştır
Anahtar Kelimeler
Gamet hücresi kryoprezervation kryoprotektan kryoprotektif etki
Kaynakça
- 1. Alvarenga MA, Alvarenga FC, Moreıra RM, Cesarıno MM (2000): Acrozomal ultrastructure of stallion spermatozoa cryopreserved with ethylene glycol using two packing systems. Equine Vet J, 32, 541-545.
- 2. Alvarenga MA, Papa FO, Landim-alvarenga FC, Medeiros ASL (2005): Amides as cryoprotectants for freezing stallion semen: a review. Anim Reprod Sci (Basımda).
- 3. An TZ, Iwakiri M, Edashige K, Takashi M (2000): Factors affecting the survival of frozen mouse spermatozoa. Cryobiology, 40, 237-249.
- 4. Arav A, Hehu D, Mattioli M (1993): Osmotic and cytotoxic study of vitrification of ımmature bovine oocytes. J Reprod Fertil, 99, 353-358.
- 5. Ball BA, Vo A (2001): Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential. J Androl, 22, 1061-1069.
- 6. Cabria E, Anel L, Herraez MP (2001): Effect of external cryoprotectants as membrane stabilizers on cryopreserved raınbow trout sperm. Theriogenology, 52, 623-635.
- 7. Chalah T, Seigneurin F, Blesbois E, Brillard JP (1999): In vitro comparison of fowl sperm viability ın ejaculates frozen by threee different techniques and relationship with subsequent fertility in vitro. Cryobiology, 39, 185-191.
- 8. Chen Y, Foote RH, Brockett CC (1993): Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull sperm. Cryobiology, 30, 423- 431.
- 9. Cheng A, Merz KM (1997): Ice- binding mechanism of winter flounder antifreeze proteins. Biophys J, 73, 2851- 2873.
- 10. Curry MR, Watson PF (1994): Osmotic effects on ram and human sperm membranes in relation to thawing injury. Cryobiology, 31, 39-46.
- 11. Dinnyes A, Urbanyi B, Baranyai B, Magyary I (1998): Chilling sensitivity of carp (cyprinus carpio) embryos at different developmental stages in the presence or absence of cryoprotectants: work in progress. Theriogenology, 50, 1-13.
- 12. Dobrinsky JR (2002): Advancements in cryopreservation of domestic animal embryos. Theriogenology, 57, 285-302.
- 13. Donoghue AM, Wishart GJ (2000): Storage of poultry semen. Anim Reprod Sci, 62, 213-232.
- 14. Edashige K, Yamaji Y, Kleinhans FW, Kasai M (2003): Artificial expression of aquaporin-3 ımproves the survival of mouse oocytes after cryopreservation. Biol Reprod, 68, 87-94.
- 15. Gao DY, Ashworth E, Watson PF, Kleınhans FW, Mazur P, Crıster, JK (1993): Hyperosmotic tolerance of human spermatozoa: separate effects of glycerol, sodium chloride, and sucrose on spermolysis. Biol Reprod, 49, 112-23.
- 16. Gomes GM, Jacop JCF, Medeiros ASL, Papa FO, Alvarenga MA (2002): Improvement of stallion spermatozoa preservation with alternative cryoprotectants for the mangalarga marchador breed. Theriogenology, 58, 277-279.
- 17. Holt WT (2000): Fundemental aspects of sperm cryobiology: theiımportance of species and individual differences. Theriogenology, 53, 47-58.
- 18. Holt, WT (2000): Basic aspects of frozen storage of semen. Anim Reprod Sci, 62,3-22.
- 19. Kasai M (1996): Simple and efficient methods for vitrification of mammalian embryos. Anim Reprod Sci, 42, 67-75.
- 20. Kasai M (2002): Advaces in the cryopreservation of mammalian oocytes and embryos: development of ultrarapid vitrification. Reprod Med Biol, 1, 1-9.
- 21. Katkov II, Katkova N, Crister JK, Mazur P (1998): Mouse spermatozoa in high concentrations of glycerol: chemical toxicity vs osmotic shock at normal and reduced oxygen concentrations. Cryobiology, 37, 325-338.
- 22. Leeuw FED, Leeuw AMD, Daas JHG, Colenbrander BV, Erkleij AJ (1993): Effects of various cryoprotective agents and membrane-stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology, 30, 32-44.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Mart 2007 |
| Yayımlandığı Sayı | Yıl 2007Cilt: 54 Sayı: 1 |