Öz
In tests, Aeromonas hydrophila and Vibrio anguillarum isolated from fish were exposed to different concentrations of benzalkonium chloride, glutaraldehyde, formaldehyde and Iodine in 1-60 minutes contact time, in the presence of organic substance. Phenol index was used to evaluate the effectiveness of 2-aminophenol. 1:500 concentration of benzalkonium chloride killed A. hydrophila in 1 minute, V. anguillarum in 5 minute. A. hydrophila and V. anguillarum were inactivated by 3:1000 concentration of glutaraldehyde in 1 minute. 37:5000 concentration of formaldehyde killed A. hydrophila in 1 minute, V. anguillarum in 5 minutes. 10 ppm concentration of iodine killed all organisms in 1 minute. None of organisms were completely killed by 1/2500 concentration of benzalkonium chloride, 3:5000 concentration of glutaraldehyde, 37:40000 concentration of formaldehyde and 1 ppm concentration of iodine. As phenol index, the activitiy of 2-aminophenol was 3.0 and 3.3 times more than phenol for, A. hydrophila, V. anguillarum. As a conclusion, iodine was found to be the most efective disinfectant against bacterial fish pathogens examined in the study, in the presence of organic matterial. Since the killing times of any disinfectant in low concentration varies in different bacterial species, it was determined that disinfectant activity should be tested for target organisms before use in the field
Anahtar Kelimeler
Kaynakça
- Aarestrup FM, Hasman H (2004): Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection. Vet Microbiol, 100, 83– 89
- Akbar-Khanzadeh F, Vaquerano MU, Akbar- Khanzadeh M, Bisesi MS (1994): Formaldehyde exposure, acute pulmonary response, and exposure control optıons in a gross anatomy labrotory. Am J Ind Med, 26, 61-75
- Arda M (1997): Mikrobiyal üremenin kontrolü. 92-99 Temel Mikrobiyoloji, 1.Baskı Medisan Yayınevi, Ankara
- Bass GK (1977): Methods of testing disinfectants. 49-77 In: SS Block (Ed), Disinfection, Sterilization and Preservation. 2th ed. Lea & Febiger, Philadelphia.
- Diker KS, Erdeğer J, Hashımoto K (1989): Effect of dısinfectants on Haemophılus somnus. Etlik Vet Mikrobiyol Derg, 6, 137-141
- Gradeel KO, Randall L, Sayers AR, Davies RH (2005): Possible associations between Salmonella persistence in poultry houses and resistance to commonly used disinfectants and putative role of mar. Vet Microbiol, 107, 127-138.
- Jeffrey DJ (1995): Chemicals used as disinfectants: active ingredients and enhancing additives. Rev Sci Tech Off Int Epiz, 14, 57-74.
- Lambert RJM (2001): Advances in disinfection testing and modelling. J Appl Microbiol, 91, 351-363.
- Luppens SB, Rombouts FM, Abee T (2002): The effect of the growth phase of Staphylococcus aureus on resistance to disinfectants in a suspension test. J Food Protect, 65, 124-129.
- Maris P (1995): Modes of action of disinfectants. Rev Sci Tech Off Int Epiz, 14, 47-55.
- Miles AA, Misra SS (1938): The estimation of the bactericidal power of blood. J Hyg, 38, 732-749.
- Moretro T, Midtgaard ES, Nesse LL, Langsrud S (2003): Susceptibility of Salmonella isolated from fish feed factories to disinfectants and air-drying at surfaces.Vet Microbiol, 94, 207-217.
- Payne DN, Babb JR, Bradly CR (1999): An evaluation of the suitability of the Europen Ssuspension test to reflect in vitro activity of antiseptics aganist clinicially significant organisms. Lett Apply Microbiol, 28, 7-12.
- Rutala WA (1996): APIC guideline for selection and use of disinfectans. Am J Infect. Control, 24, 313-342
- Rutala WA, Weber DJ (1997): Uses of inorganic hypochlorite (blech) in health care facilities. Clin Microbio Rev, 10, 597-610
- Rutala WA, Gergen MF, Weber DJ (1993): Inactivation of Clostridium difficale spores by disenfectants. Infect Control Hosp Epidemiol, 14, 36-39
- Wirtanen G, Salo S, Helander IM, Matilla-Sandholm T (2001): Microbiological methods for testing disinfectant efficiency on Pseudomonas biofilm. Colloids Surf.B Biointerfaces, 20, 37-50.
Öz
Çalışmada, balıklardan izole edilen Aeromonas hydrophila ve Vibrio anguillarum farklı konsantrasyonlardaki benzalkonyum klorür, glutaraldehid, formaldehid ve iyot’a 1-60 dakika temas süresinde, organik madde varlığında maruz bırakıldı. Fenol indeksi 2-Aminofenol’ün etkinliğinin değerlendirilmesinde kullanıldı. Benzalkonyum klorür’ün 1:500’lük konsantrasyonu, A. hydrophila’yı 1 dakikada, V. anguillarum’u 5 dakikada öldürdü. A. hydrophila ve V. anguillarum glutaraldehid’in 3:1000’lik konsantrasyonu tarafından 1 dakikada inaktive edildi. Formaldehid’in, 37:5000’lik konsantrasyonu, A. hydrophila’yı 1 dakikada, V. anguillarum’u 5 dakikada öldürdü. İyot’un 10 ppm’lik konsantrasyonu, bütün organizmaları 1 dakikada öldürdü. Organizmalardan hiç biri benzalkonyum klorür’ün 1:25000’lik, glutaraldehid’in 3:5000’lik, formaldehid’in 37:40000’lik ve iyot’un 1 ppm’lik konsantrasyonu tarafından tamamen öldürülmedi. Fenol indeksi olarak, 2-aminofenol’ün etkisi, A. hydrophila, V. anguillarum için fenole göre 3.0 ve 3.3 kattır. Sonuç olarak, iyot organik madde varlığında çalışmada incelenen balık bakteriyel patojenlerine karşı en etkili dezenfektan olarak bulundu. Düşük konsantrasyondaki herhangi bir dezenfektanın öldürme zamanı farklı bakteri türlerinde değiştiği için sahada kullanmadan önce dezenfektan aktivitesinin hedef organizma için test edilmesinin gerektiği belirlendi
Anahtar Kelimeler
Kaynakça
- Aarestrup FM, Hasman H (2004): Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection. Vet Microbiol, 100, 83– 89
- Akbar-Khanzadeh F, Vaquerano MU, Akbar- Khanzadeh M, Bisesi MS (1994): Formaldehyde exposure, acute pulmonary response, and exposure control optıons in a gross anatomy labrotory. Am J Ind Med, 26, 61-75
- Arda M (1997): Mikrobiyal üremenin kontrolü. 92-99 Temel Mikrobiyoloji, 1.Baskı Medisan Yayınevi, Ankara
- Bass GK (1977): Methods of testing disinfectants. 49-77 In: SS Block (Ed), Disinfection, Sterilization and Preservation. 2th ed. Lea & Febiger, Philadelphia.
- Diker KS, Erdeğer J, Hashımoto K (1989): Effect of dısinfectants on Haemophılus somnus. Etlik Vet Mikrobiyol Derg, 6, 137-141
- Gradeel KO, Randall L, Sayers AR, Davies RH (2005): Possible associations between Salmonella persistence in poultry houses and resistance to commonly used disinfectants and putative role of mar. Vet Microbiol, 107, 127-138.
- Jeffrey DJ (1995): Chemicals used as disinfectants: active ingredients and enhancing additives. Rev Sci Tech Off Int Epiz, 14, 57-74.
- Lambert RJM (2001): Advances in disinfection testing and modelling. J Appl Microbiol, 91, 351-363.
- Luppens SB, Rombouts FM, Abee T (2002): The effect of the growth phase of Staphylococcus aureus on resistance to disinfectants in a suspension test. J Food Protect, 65, 124-129.
- Maris P (1995): Modes of action of disinfectants. Rev Sci Tech Off Int Epiz, 14, 47-55.
- Miles AA, Misra SS (1938): The estimation of the bactericidal power of blood. J Hyg, 38, 732-749.
- Moretro T, Midtgaard ES, Nesse LL, Langsrud S (2003): Susceptibility of Salmonella isolated from fish feed factories to disinfectants and air-drying at surfaces.Vet Microbiol, 94, 207-217.
- Payne DN, Babb JR, Bradly CR (1999): An evaluation of the suitability of the Europen Ssuspension test to reflect in vitro activity of antiseptics aganist clinicially significant organisms. Lett Apply Microbiol, 28, 7-12.
- Rutala WA (1996): APIC guideline for selection and use of disinfectans. Am J Infect. Control, 24, 313-342
- Rutala WA, Weber DJ (1997): Uses of inorganic hypochlorite (blech) in health care facilities. Clin Microbio Rev, 10, 597-610
- Rutala WA, Gergen MF, Weber DJ (1993): Inactivation of Clostridium difficale spores by disenfectants. Infect Control Hosp Epidemiol, 14, 36-39
- Wirtanen G, Salo S, Helander IM, Matilla-Sandholm T (2001): Microbiological methods for testing disinfectant efficiency on Pseudomonas biofilm. Colloids Surf.B Biointerfaces, 20, 37-50.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Eylül 2006 |
| Yayımlandığı Sayı | Yıl 2006Cilt: 53 Sayı: 3 |