Öz
Bu çalışmada, ksantin oksidaz inhibitörü olan allopurinolün gentamisin nefrotoksikozisinde koruyucu etkinliğinin araştırılması amaçlanmıştır.Gentamisin verilen grubun serum üre ve kreatinin düzeyleri ile plazma MDA düzeyi kontrol grubuna göre istatistiksel olarak önemli düzeyde yüksek bulunurken, eritrositlerde GSH-Px, SOD aktiviteleri düşük bulundu. Gentamisin+Allopurinol verilen grubun serum üre ve kreatinin düzeyleri ile plazma MDA düzeyi kontrol grubuna göre önemli düzeyde yüksek bulunurken, GSH-Px, SOD ve CAT aktiviteleri önemli düzeyde düşüktü. Gentamisin verilen grubun plazma MDA düzeyi gentamisin+allopurinol verilen gruba göre önemli düzeyde düşük iken GSH-Px, SOD aktiviteleri önemli düzeyde yüksekti. Sonuçta ksantin oksidaz inhibitörü olan allopurinolün gentamisin nefrotoksikozisinin oluşumunu önleyemediği kanısına varılmıştır
Anahtar Kelimeler
Kaynakça
- Ciz M, Cizova H, Lojek A, Kubala L, Papezikova I (2001): Ischemia/Reperfusion Injury of Rat Small Intestine: The Effect of Allopurinol Dosage. Transplant P, 33, 2871–2873.
- Culling CFA, Allison RA, Barr WT (1985): Cellular Pathology Technicgue. Mid-Country Press, London.
- Diamond JR, Bonventre JV, Karnovsky MJ (1986): A role for oxygen free radicals in aminonucleoside nephrosis. Kidney Int, 29, 478-483.
- Faure M, Lissi EA, Videla LA (1990): Antioxidant capacity of allopurinol in biological systems. Biochem Int, 21, 357-366.
- Greene EL, Paller MS (1991): Oxygen free radicals in acute renal failure. Miner Electrolyte Metab, 17, 124-132.
- Hande K, Reede E, Chabner B (1978): Allopurinol Kinetics. Clin Pharmacol Ther, 23, 598-605.
- Kavutcu M, Canbolat O, Öztürk S, Olcay E, Ulutepe S, Ekinci C, Gökhun IH, Durak I (1996): Reduced enzymatic antioxidant defence mechanism in kidney tissues from gentamicin-treated guine pigs: Effects of vitamin E and C. Nephron, 72, 269-274.
- Kumar KV, Shifow AA, Naidu MUR, Ratnakar KS (2000): Carvedilol: a beta blocker with antioxidant property protects against gentamicin-induced nephrotoxicity in rats. Life Sci, 66, 2603-2611.
- Luck H (1965): Catalase. 855-884. In: HU Bergmeyer (Ed.), Methods in Analysis. Academic Press Inc, London.
- Maestro RF (1980): An approach to free radicals in medicine and biology. Acta Physiol Scand, 492, 153.
- Maxie MG, Prescott JF. (1993):Urinary System. 487- 491, In: KVF Jubb, PC Kennedy, N Palmer (Eds), Pathology of Domestic Animals. Academic Press, New York.
- Paglia DE, Valentine WN (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
- Richter C (1995): Oxidative damage to mitochondrial DNA and its relationship to ageing. Int J Biochem Cell Biol, 27, 647-653.
- Sun Y, Oberley LW, Li Y (1988): A simple for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
- Thiemermann C, Bowes J, Myint FP, Vane JR (1997): Inhibition of the activity of poly(ADP ribose) synthetase reduces ischemia-reperfusion injury in the heart and skeletal muscle. Proc Natl Acad Sci USA, 94, 679-683.
- Tietz WN (1987): Fundamental of Clinical Chemistry. WB Saunders Co. Philadelphia.
- Wexler BC, Greenberg BP (1978): Allopurinol-induced myocardial and renal damage in nonarteriosclerotic (virgin) and arteriosclerotic (breeder) Sprague-Dawley rats. Proc Soc Exp Biol Med, 157, 541-549.
- Whiting PH, Brown PA (1996): The relationship between enzymuria and kidney enzyme activities in experimental gentamicin nephrotoxicity. Ren Fail, 18, 899-909.
- Winterbourn CC, Hawkins RE, Brain M, Carrel W (1975): The estimation of red cell superoxide dismutase activity. J Lab Clin Med, 55, 337-341.
- Yoshioka T, Kawoda K, Shimoda T (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet. Gynecol, 135, 372-376.
- Zager RA, Gmur DJ (1989): Effects of xanthine oxidase inhibition on ischemic acute renal failure in the rat. Am J Physiol, 257, 953-958.
Öz
In this study, it is purposed to investigate the protective effects of allopurinol, a xanthine oxidase inhibitor, against gentamicin -induced nephrotoxicosis. The levels of serum urea, creatinine and plasma MDA were significantly higher and GSH-Px, SOD activities were lower in gentamicin administrated group than the control. The levels of serum urea, creatinine and plasma MDA were significantly higher and GSH-Px, SOD and CAT activities were significantly lower in gentamicin + allopurinol administrated group than the control. Plasma MDA was significantly lower and GSH-Px, SOD activities were higher in gentamicin induced group than gentamicin + allopurinol induced group. In conclusion, results demonstrate that allopurinol, a xanthine oxidase inhibitor, does not prevent the formation of gentamicin induced nephrotoxicosis
Anahtar Kelimeler
Kaynakça
- Ciz M, Cizova H, Lojek A, Kubala L, Papezikova I (2001): Ischemia/Reperfusion Injury of Rat Small Intestine: The Effect of Allopurinol Dosage. Transplant P, 33, 2871–2873.
- Culling CFA, Allison RA, Barr WT (1985): Cellular Pathology Technicgue. Mid-Country Press, London.
- Diamond JR, Bonventre JV, Karnovsky MJ (1986): A role for oxygen free radicals in aminonucleoside nephrosis. Kidney Int, 29, 478-483.
- Faure M, Lissi EA, Videla LA (1990): Antioxidant capacity of allopurinol in biological systems. Biochem Int, 21, 357-366.
- Greene EL, Paller MS (1991): Oxygen free radicals in acute renal failure. Miner Electrolyte Metab, 17, 124-132.
- Hande K, Reede E, Chabner B (1978): Allopurinol Kinetics. Clin Pharmacol Ther, 23, 598-605.
- Kavutcu M, Canbolat O, Öztürk S, Olcay E, Ulutepe S, Ekinci C, Gökhun IH, Durak I (1996): Reduced enzymatic antioxidant defence mechanism in kidney tissues from gentamicin-treated guine pigs: Effects of vitamin E and C. Nephron, 72, 269-274.
- Kumar KV, Shifow AA, Naidu MUR, Ratnakar KS (2000): Carvedilol: a beta blocker with antioxidant property protects against gentamicin-induced nephrotoxicity in rats. Life Sci, 66, 2603-2611.
- Luck H (1965): Catalase. 855-884. In: HU Bergmeyer (Ed.), Methods in Analysis. Academic Press Inc, London.
- Maestro RF (1980): An approach to free radicals in medicine and biology. Acta Physiol Scand, 492, 153.
- Maxie MG, Prescott JF. (1993):Urinary System. 487- 491, In: KVF Jubb, PC Kennedy, N Palmer (Eds), Pathology of Domestic Animals. Academic Press, New York.
- Paglia DE, Valentine WN (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
- Richter C (1995): Oxidative damage to mitochondrial DNA and its relationship to ageing. Int J Biochem Cell Biol, 27, 647-653.
- Sun Y, Oberley LW, Li Y (1988): A simple for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
- Thiemermann C, Bowes J, Myint FP, Vane JR (1997): Inhibition of the activity of poly(ADP ribose) synthetase reduces ischemia-reperfusion injury in the heart and skeletal muscle. Proc Natl Acad Sci USA, 94, 679-683.
- Tietz WN (1987): Fundamental of Clinical Chemistry. WB Saunders Co. Philadelphia.
- Wexler BC, Greenberg BP (1978): Allopurinol-induced myocardial and renal damage in nonarteriosclerotic (virgin) and arteriosclerotic (breeder) Sprague-Dawley rats. Proc Soc Exp Biol Med, 157, 541-549.
- Whiting PH, Brown PA (1996): The relationship between enzymuria and kidney enzyme activities in experimental gentamicin nephrotoxicity. Ren Fail, 18, 899-909.
- Winterbourn CC, Hawkins RE, Brain M, Carrel W (1975): The estimation of red cell superoxide dismutase activity. J Lab Clin Med, 55, 337-341.
- Yoshioka T, Kawoda K, Shimoda T (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet. Gynecol, 135, 372-376.
- Zager RA, Gmur DJ (1989): Effects of xanthine oxidase inhibition on ischemic acute renal failure in the rat. Am J Physiol, 257, 953-958.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Mart 2006 |
| Yayımlandığı Sayı | Yıl 2006Cilt: 53 Sayı: 1 |