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The effect of follicle stimulating hormone and human menopausal gonadotropin on in vitro maturation of bovine oocytes

Year 2006, Volume: 53 Issue: 1, 25 - 29, 01.03.2006

Hasan Ceyhun Macun Mustafa Kaymaz

https://doi.org/10.1501/Vetfak_0000000064

Abstract

The aim of this study was to investigate the effects of FSH (Follicle Stimulating Hormone) and hMG (human Menopausal Gonadotropin) which are both easy to obtain and cheap when compared to other gonadotropins, on in vitro maturation of bovine oocytes. According to hormone added to in vitro maturation media, three groups were formed; Group I (FSH), Group II (hMG) and Group III (Control). After the in vitro maturation, Giemsa staining of oocytes in group I revealed that 14.36% of oocytes were at GV (Germinal Vesicle) stage, 6.38% of oocytes were at M I (Metaphase I) stage and 76.07% of oocytes were at M II stage while 3.19% of oocytes were degenerated. The percentages of oocytes at stages GV, M I, M II and degenerated oocytes in groups II and III were 11.40%, 15.54%, 66.84%, 6.22% and 23.03%, 6.06%, 64.24%, 6.67% respectively. Distribution of oocytes into different stages within the groups was significantly different (p

Keywords

Bovine follicle stimulating hormone human menopausal gonadotropin in vitro maturation oocyte

References

  • Beker ARCL, Colenbrander B, Bevers MM (2002): Effect of 17β estradiol on the in vitro maturation of bovine oocytes. Theriogenology, 58, 1663-1673.
  • Beker ARCL, Izadyar F, Colenbrander B, Bevers MM (2000): Effect of growth hormone releasing hormone (GHRH) and vasoactive intestinal peptide (VIP) on in vitro bovine oocyte maturation. Theriogenology, 53, 1771-1782.
  • Brackett BG, Zuelke KA (1993): Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology, 39, 43-64.
  • Calder MD, Caveney AN, Westhusin ME, Watson AJ (2001): Cyclooxygenase-2 and prostaglandin E2 (PGE2) receptor messenger RNAs are affected by bovine oocyte maturation time and cumulus-oocyte complex quality, and PGE2 induces moderate expansion of the bovine cumulus in vitro. Biol Reprod, 65, 135-140.
  • Choi YH, Carnevale EM, Seidel GE, Squires EL (2001): Effectes of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology, 56, 661-670.
  • Çetin Y (2004): İmmatür Sığır Oositlerinin Vitrifikasyon Tekniği ile Etilen Glikol ve Dmso Kullanarak Payetlerde Dondurulması. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü.
  • Döcke F (1994): Wirkungen der keimdrüsenhormone. 418- 508. In: F Döcke (Ed), Veterinärmedizinische Endokrinologie. Gustav Fischer Verlag Jena, Stuttgart.
  • Gordon I (1994): Laboratory Production of Cattle Embryos. CAB International, Wallingford.
  • Greve T, Madison V (1991): In vitro fertilization in cattle: a review. Reprod Nutr Dev, 31, 147-157.
  • Greve T, Madison V, Avery B, Callesen H, Hyttel P (1993): In vitro production of bovine embryos: A progress report and the consequences on the genetic upgrading of cattle populations. Anim Reprod Sci, 33, 51-69.
  • Kaya ZP, Ün M (2003): Sığır oositlerinin in vitro maturasyonunun belirlenmesinde kullanılan kriterlerin et- kinliğinin karşılaştırılması. I. Türk Veteriner Jinekoloji Kongresi Konya, 62.
  • Landim AFC, Boyazoglu SEA, Carvalho LR, Choi YH, Squires EL, Seidel GE (2002): Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle. Anim Reprod Sci, 71, 181-191.
  • Leibfried RML, Critser ES, Parrish JJ, First NL (1989): In vitro maturation and fertilization of bovine oocytes. Theriogenology, 31, 61-74.
  • Liu JM, Jin ZQ, Zhao XX, Zhu YD (1991): The development of bovine follicular oocytes matured in different culture media. Vet Res Com, 15, 257-260.
  • Lorenzo PL, Illera MJ, Illera JC, Illera M (1995): Role of EGF, IGF-I, sera and cumulus cells on maturation in vitro of bovine oocytes. Theriogenology, 44, 109-118.
  • Luciano AM, Modina S, Vassena R, Milanesi E, Lauria A, Gandolfi F (2004): Role of intracellular cyclic adenosine 3’,5’-monophosphate concentration and oocyte- cumulus cells communications on the acquisition of the developmental competence during in vitro maturation of bovine oocyte. Biol Reprod, 70, 465-472.
  • Mikkelsen AL, Smith S, Lindenberg S (2000): Possible factors affecting the development of oocytes in in-vitro maturation. Hum Reprod, 15, 11-17.
  • Niemann H, Meinecke B (1993): In vitro produktion von embryonen. 86-103. In: H Niemann, B Meinecke (Ed), Embryotransfer und Assoziierte Biotechniken bei Landwirthschaftlichen Nutztieren. Ferdinand Enke Verlag, Stuttgart.
  • Ocana QJM, Merlin MP, Ortega MM, Millan MM (1999): The effect of different sera and bovine serum albumin fraction (BSA) on in vitro maturation of immature bovine oocytes. Arch Zootec, 48, 167-174.
  • Ocana QJM, Millan MM, Cordoba MV, Franganillo AR (1994): The influence of different types of media supplement on the meiotic maturation of bovine oocytes in vitro. Theriogenology, 41, 405-411.
  • Rieger D, Luciano AM, Modina S, Pocar P, Lauria A, Gandolfi F (1998): The effects of epidermal growth factor and insulin-like growth factor I on the metabolic activity, nuclear maturation and subsequent development of cattle oocytes in vitro. J Reprod Fertil, 112, 123-130.
  • Sanbuissho A, Threlfall WR (1990): The influence of serum and gonadotropins on in vitro maturation and fertilization of bovine oocytes. Theriogenology, 34, 341- 348.
  • Shamsuddin M, Larsson B, Rodriguez MH (1993): Maturation-related changes in bovine oocytes under different culture conditions. Anim Reprod Sci, 31, 49-60.
  • Sirard MA, Blondin P (1996): Oocyte maturation and IVF in cattle. Anim Reprod Sci, 42, 417-426.
  • Soom AV, Kruif AD (1996): Oocyte maturation, sperm capacitation and pre-implantation development in the bovine: Implications for in vitro production of embryos. Reprod Dom Anim, 31, 687-701.
  • Thompson JG (1997): Comparison between in vivo- derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod Fertil Dev, 9, 341-354.
  • Trounson A (1992): The production of ruminant embryos in vitro. Anim Reprod Sci, 28, 125-137.
  • Ün ZP (2002). Östrustaki Sığır Serumu ve Fetuin’in Sığır Oositlerinin in vitro Maturasyonu Üzerine Etkileri. Dokto- ra Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü.
  • Wit AAC, Wurth YA, Kruip AM (2000): Effect of ovarian phase and follicle quality on morphology and developmental capacity of the bovine cumulus-oocyte complex. J Anim Sci, 78, 1277-1283.
  • Yang X, Kubota C, Suzuki H, Taneja M, Bols PEJ, Presicce GA (1998): Control of oocyte maturation in cows-biological factors. Theriogenology, 49, 471-482.
  • Younis AI, Brackett BG, Fayrer HRA (1989): Influence of serum and hormones on bovine oocyte maturation and fertilization in vitro. Gamete Res, 23, 189-201.
  • Zuelke KA, Brackett BG (1992): Effects of luteinizing hormone on glucose metabolism in cumulus-enclosed bovine oocytes matured in vitro. Endocrinology, 131, 2690-2696

Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi

Year 2006, Volume: 53 Issue: 1, 25 - 29, 01.03.2006

Hasan Ceyhun Macun Mustafa Kaymaz

https://doi.org/10.1501/Vetfak_0000000064

Abstract

Bu çalışmanın amacı; benzer gonadotropinlere göre daha ucuz olan ve kolay bulunabilen FSH (Follikül Uyarıcı Hormon) ve hMG (İnsan Menopozal Gonadotropini) hormonlarının sığır oositlerindeki in vitro maturasyon oranlarına etkisinin araştırılmasıdır. Oositlerin in vitro maturasyonu sırasında vasatlara eklenen hormonlara göre; FSH kullanılan Grup 1, hMG kullanılan Grup 2 ve kontrol grubu (Grup 3) olmak üzere 3 grup oluşturuldu. In vitro maturasyondan sonra, Grup 1’de Giemsa ile boyaması yapılan oositlerin %14.36’sının GV (Germinal Vezikül), %6.38’inin M I (Metafaz I), %76,07’sinin M II döneminde olduğu belirlenirken, %3.19’unun dejenere olduğu tespit edildi. Bu durum 2. ve 3. Grupta sırasıyla %11.40; %15.54; %66.84; %6.22 ve %23.03; %6.06; %64.24; %6.67 olarak belirlendi. Gruplarda oluşan dağılımların birbirinden farklı olduğu tespit edildi (p

Keywords

Follikül uyarıcı hormon insan menapozal gonadotropin in vitro maturasyon oosit sığır

References

  • Beker ARCL, Colenbrander B, Bevers MM (2002): Effect of 17β estradiol on the in vitro maturation of bovine oocytes. Theriogenology, 58, 1663-1673.
  • Beker ARCL, Izadyar F, Colenbrander B, Bevers MM (2000): Effect of growth hormone releasing hormone (GHRH) and vasoactive intestinal peptide (VIP) on in vitro bovine oocyte maturation. Theriogenology, 53, 1771-1782.
  • Brackett BG, Zuelke KA (1993): Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology, 39, 43-64.
  • Calder MD, Caveney AN, Westhusin ME, Watson AJ (2001): Cyclooxygenase-2 and prostaglandin E2 (PGE2) receptor messenger RNAs are affected by bovine oocyte maturation time and cumulus-oocyte complex quality, and PGE2 induces moderate expansion of the bovine cumulus in vitro. Biol Reprod, 65, 135-140.
  • Choi YH, Carnevale EM, Seidel GE, Squires EL (2001): Effectes of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology, 56, 661-670.
  • Çetin Y (2004): İmmatür Sığır Oositlerinin Vitrifikasyon Tekniği ile Etilen Glikol ve Dmso Kullanarak Payetlerde Dondurulması. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü.
  • Döcke F (1994): Wirkungen der keimdrüsenhormone. 418- 508. In: F Döcke (Ed), Veterinärmedizinische Endokrinologie. Gustav Fischer Verlag Jena, Stuttgart.
  • Gordon I (1994): Laboratory Production of Cattle Embryos. CAB International, Wallingford.
  • Greve T, Madison V (1991): In vitro fertilization in cattle: a review. Reprod Nutr Dev, 31, 147-157.
  • Greve T, Madison V, Avery B, Callesen H, Hyttel P (1993): In vitro production of bovine embryos: A progress report and the consequences on the genetic upgrading of cattle populations. Anim Reprod Sci, 33, 51-69.
  • Kaya ZP, Ün M (2003): Sığır oositlerinin in vitro maturasyonunun belirlenmesinde kullanılan kriterlerin et- kinliğinin karşılaştırılması. I. Türk Veteriner Jinekoloji Kongresi Konya, 62.
  • Landim AFC, Boyazoglu SEA, Carvalho LR, Choi YH, Squires EL, Seidel GE (2002): Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle. Anim Reprod Sci, 71, 181-191.
  • Leibfried RML, Critser ES, Parrish JJ, First NL (1989): In vitro maturation and fertilization of bovine oocytes. Theriogenology, 31, 61-74.
  • Liu JM, Jin ZQ, Zhao XX, Zhu YD (1991): The development of bovine follicular oocytes matured in different culture media. Vet Res Com, 15, 257-260.
  • Lorenzo PL, Illera MJ, Illera JC, Illera M (1995): Role of EGF, IGF-I, sera and cumulus cells on maturation in vitro of bovine oocytes. Theriogenology, 44, 109-118.
  • Luciano AM, Modina S, Vassena R, Milanesi E, Lauria A, Gandolfi F (2004): Role of intracellular cyclic adenosine 3’,5’-monophosphate concentration and oocyte- cumulus cells communications on the acquisition of the developmental competence during in vitro maturation of bovine oocyte. Biol Reprod, 70, 465-472.
  • Mikkelsen AL, Smith S, Lindenberg S (2000): Possible factors affecting the development of oocytes in in-vitro maturation. Hum Reprod, 15, 11-17.
  • Niemann H, Meinecke B (1993): In vitro produktion von embryonen. 86-103. In: H Niemann, B Meinecke (Ed), Embryotransfer und Assoziierte Biotechniken bei Landwirthschaftlichen Nutztieren. Ferdinand Enke Verlag, Stuttgart.
  • Ocana QJM, Merlin MP, Ortega MM, Millan MM (1999): The effect of different sera and bovine serum albumin fraction (BSA) on in vitro maturation of immature bovine oocytes. Arch Zootec, 48, 167-174.
  • Ocana QJM, Millan MM, Cordoba MV, Franganillo AR (1994): The influence of different types of media supplement on the meiotic maturation of bovine oocytes in vitro. Theriogenology, 41, 405-411.
  • Rieger D, Luciano AM, Modina S, Pocar P, Lauria A, Gandolfi F (1998): The effects of epidermal growth factor and insulin-like growth factor I on the metabolic activity, nuclear maturation and subsequent development of cattle oocytes in vitro. J Reprod Fertil, 112, 123-130.
  • Sanbuissho A, Threlfall WR (1990): The influence of serum and gonadotropins on in vitro maturation and fertilization of bovine oocytes. Theriogenology, 34, 341- 348.
  • Shamsuddin M, Larsson B, Rodriguez MH (1993): Maturation-related changes in bovine oocytes under different culture conditions. Anim Reprod Sci, 31, 49-60.
  • Sirard MA, Blondin P (1996): Oocyte maturation and IVF in cattle. Anim Reprod Sci, 42, 417-426.
  • Soom AV, Kruif AD (1996): Oocyte maturation, sperm capacitation and pre-implantation development in the bovine: Implications for in vitro production of embryos. Reprod Dom Anim, 31, 687-701.
  • Thompson JG (1997): Comparison between in vivo- derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod Fertil Dev, 9, 341-354.
  • Trounson A (1992): The production of ruminant embryos in vitro. Anim Reprod Sci, 28, 125-137.
  • Ün ZP (2002). Östrustaki Sığır Serumu ve Fetuin’in Sığır Oositlerinin in vitro Maturasyonu Üzerine Etkileri. Dokto- ra Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü.
  • Wit AAC, Wurth YA, Kruip AM (2000): Effect of ovarian phase and follicle quality on morphology and developmental capacity of the bovine cumulus-oocyte complex. J Anim Sci, 78, 1277-1283.
  • Yang X, Kubota C, Suzuki H, Taneja M, Bols PEJ, Presicce GA (1998): Control of oocyte maturation in cows-biological factors. Theriogenology, 49, 471-482.
  • Younis AI, Brackett BG, Fayrer HRA (1989): Influence of serum and hormones on bovine oocyte maturation and fertilization in vitro. Gamete Res, 23, 189-201.
  • Zuelke KA, Brackett BG (1992): Effects of luteinizing hormone on glucose metabolism in cumulus-enclosed bovine oocytes matured in vitro. Endocrinology, 131, 2690-2696
There are 32 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Hasan Ceyhun Macun

Mustafa Kaymaz

Publication Date March 1, 2006
Published in Issue Year 2006Volume: 53 Issue: 1

Cite

APA Macun, H. C., & Kaymaz, M. (2006). Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 53(1), 25-29. https://doi.org/10.1501/Vetfak_0000000064
AMA Macun HC, Kaymaz M. Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi. Ankara Univ Vet Fak Derg. March 2006;53(1):25-29. doi:10.1501/Vetfak_0000000064
Chicago Macun, Hasan Ceyhun, and Mustafa Kaymaz. “Sığır Oositlerinin in Vitro Maturasyonuna follikül uyarıcı Hormon Ve Insan Menopozal Gonadotropininin Etkisi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53, no. 1 (March 2006): 25-29. https://doi.org/10.1501/Vetfak_0000000064.
EndNote Macun HC, Kaymaz M (March 1, 2006) Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53 1 25–29.
IEEE H. C. Macun and M. Kaymaz, “Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi”, Ankara Univ Vet Fak Derg, vol. 53, no. 1, pp. 25–29, 2006, doi: 10.1501/Vetfak_0000000064.
ISNAD Macun, Hasan Ceyhun - Kaymaz, Mustafa. “Sığır Oositlerinin in Vitro Maturasyonuna follikül uyarıcı Hormon Ve Insan Menopozal Gonadotropininin Etkisi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 53/1 (March 2006), 25-29. https://doi.org/10.1501/Vetfak_0000000064.
JAMA Macun HC, Kaymaz M. Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi. Ankara Univ Vet Fak Derg. 2006;53:25–29.
MLA Macun, Hasan Ceyhun and Mustafa Kaymaz. “Sığır Oositlerinin in Vitro Maturasyonuna follikül uyarıcı Hormon Ve Insan Menopozal Gonadotropininin Etkisi”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 53, no. 1, 2006, pp. 25-29, doi:10.1501/Vetfak_0000000064.
Vancouver Macun HC, Kaymaz M. Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi. Ankara Univ Vet Fak Derg. 2006;53(1):25-9.