BibTex RIS Kaynak Göster

Sığır oositlerinin in vitro maturasyonunun belirlenmesinde kullanılan kriterlerin etkinliğinin karşılaştırılması

Yıl 2004, Cilt: 51 Sayı: 3, 205 - 209, 01.09.2004

Zeynep Pınar Ün Mustafa Ün

Öz

Çalışmada, mezbahadan toplanan ovaryumlardan aspire edilen toplam 200 sığır oositi kullanıldı. In vitro maturasyon, %0.6 sığır serum albumini katılmış doku kültürü vasatı içerisinde, 39°C’de, %5 CO2 atmosferinde 24 saatte gerçekleştildi. In vitro fertilizasyon işlemi ise modifiye Tyrode’nin albumin laktat piruvat vasatında aynı inkubasyon koşullarında 20 saatte gerçekleştirildi. Çalışmaya alınan oositler 10’arlı gruplar halinde işaretli petrilerde inkübe edilerek, her oosit, maturasyon sonrası kumulus ekspansiyon derecesi (yok-0, hafif-1, ileri-2), kutup hücresinin görülmesi (yok-0, var-1) ve fertilizasyonun ortaya konması (dişierkek pronukleuslar yok-0, var-1) açısından değerlendirildi. Çalışma sonucunda, in vitro maturasyon ve fertilizasyon işlemine alınan toplam 200 oositten 91’inde (%45.5) fertilizasyon saptanmıştır. Kumulus ekspansiyonunu göstermeyen 42 oositten 4’ünde fertilizasyon gözlenirken (%9.5) (p

Anahtar Kelimeler

İn vitro maturasyon kumulus ekspansiyonu kutup hücresi oosit sığır

Kaynakça

  • Brackett BG, Zuelke KA (1993): Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology, 39, 43-64.
  • Gordon, I (1994): The Laboratory Production of Bovine Embryos. University Press, Cambridge.
  • Gordon I, Lu KH (1990): Production of embryos in vitro and its impacts on livestock production. Theriogenology, 33, 77-87.
  • Hoshi, H (2003): In vitro production of bovine embryos and their application for embryo transfer. Theriogenology, 59, 675-685.
  • Hunter AG, Moore RM (1987): Stage-dependent effects of inhibiting ribonucleic acids and protein synthesis on meiotic maturation of bovine oocytes in vitro. J Dairy Sci, 70, 1646-1651.
  • Kim KS, Mitsumizo N, Fujita K, Utsumi K (1996): The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos. Theriogenology, 45, 787-799.
  • Nandi S, Ravindranatha BM, Gupta PSP, Sarma PV (2002): Timing of sequential changes in cumulus cells and first polar body extrusion during in vitro maturation of buffalo oocytes. Theriogenology, 57, 1151-1159.
  • Neglia G, Gasparrini B, di Brienza VC, Palo RD, Campanile G, Presicce GA Zicarelli L (2003): Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. Theriogenology, 59, 1123-1130.
  • Nelson LD, Nelson CF (2002): Handling and culture of bovine embryos: survey of media used by 26 embryo trans- fer companies in the USA. Theriogenology, 56, 1377-1382.
  • Shamsuddin M, Larsson B, Rodriguez-Martinez H (1993): Maturation-related changes in bovine oocytes under different culture conditions. Anim Reprod Sci, 31, 49-60.
  • Sirard MA, Blondin P (1996): Oocyte maturation and IVF in cattle. Anim Reprod Sci, 42, 417-426.
  • Tanghe S, Soom AV, Mehrzad J, Maes D, Duchateau L, de Kruif A (2003): Cumulus contributions during bovine fertilization in vitro. Theriogenology, 60, 135-149.
  • Ün M (2002): Mezbahadan Toplanan Ovaryumlar-dan Elde Edilen Sığır Oositlerinin in vitro Maturasyonu ve Fertilizasyonu. Ankara Üniversitesi Sağlık Bilimleri Dok- tora Tezi, Ankara.

The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes

Yıl 2004, Cilt: 51 Sayı: 3, 205 - 209, 01.09.2004

Zeynep Pınar Ün Mustafa Ün

Öz

In this study, totally 200 bovine oocytes aspirated from the slaughtered ovaries were used as the material. In vitro maturation process was carried out in bovine serum albumine added (%0.6) tissue culture media at 39 C° under 5% CO2 atmosphere for 24 hours. In vitro fertilization was carried out in modified Tyrode’s albumine lactate pyruvate media with similar conditions for 20 hours. Oocytes were placed into seperated-tagged petri dishes in ten-a-piece and every oocytes were evaluated for the cumulus expansion degrees (none-0, slight-1 and progressive-2), extursion of polar body (none-0 and existence-1) and fertilization (male and female pronuclei absence-0 and existence-1). After the fertilization process, 91 of 200 oocytes were fertilized (45.5%). Fertilization was observed in 4 of 42 unexpanded oocytes (9.5%) (p

Anahtar Kelimeler

Bovine cumulus expansion in vitro maturation oocyte polar body

Kaynakça

  • Brackett BG, Zuelke KA (1993): Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology, 39, 43-64.
  • Gordon, I (1994): The Laboratory Production of Bovine Embryos. University Press, Cambridge.
  • Gordon I, Lu KH (1990): Production of embryos in vitro and its impacts on livestock production. Theriogenology, 33, 77-87.
  • Hoshi, H (2003): In vitro production of bovine embryos and their application for embryo transfer. Theriogenology, 59, 675-685.
  • Hunter AG, Moore RM (1987): Stage-dependent effects of inhibiting ribonucleic acids and protein synthesis on meiotic maturation of bovine oocytes in vitro. J Dairy Sci, 70, 1646-1651.
  • Kim KS, Mitsumizo N, Fujita K, Utsumi K (1996): The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos. Theriogenology, 45, 787-799.
  • Nandi S, Ravindranatha BM, Gupta PSP, Sarma PV (2002): Timing of sequential changes in cumulus cells and first polar body extrusion during in vitro maturation of buffalo oocytes. Theriogenology, 57, 1151-1159.
  • Neglia G, Gasparrini B, di Brienza VC, Palo RD, Campanile G, Presicce GA Zicarelli L (2003): Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. Theriogenology, 59, 1123-1130.
  • Nelson LD, Nelson CF (2002): Handling and culture of bovine embryos: survey of media used by 26 embryo trans- fer companies in the USA. Theriogenology, 56, 1377-1382.
  • Shamsuddin M, Larsson B, Rodriguez-Martinez H (1993): Maturation-related changes in bovine oocytes under different culture conditions. Anim Reprod Sci, 31, 49-60.
  • Sirard MA, Blondin P (1996): Oocyte maturation and IVF in cattle. Anim Reprod Sci, 42, 417-426.
  • Tanghe S, Soom AV, Mehrzad J, Maes D, Duchateau L, de Kruif A (2003): Cumulus contributions during bovine fertilization in vitro. Theriogenology, 60, 135-149.
  • Ün M (2002): Mezbahadan Toplanan Ovaryumlar-dan Elde Edilen Sığır Oositlerinin in vitro Maturasyonu ve Fertilizasyonu. Ankara Üniversitesi Sağlık Bilimleri Dok- tora Tezi, Ankara.
Toplam 13 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Zeynep Pınar Ün

Mustafa Ün

Yayımlanma Tarihi 1 Eylül 2004
Yayımlandığı Sayı Yıl 2004Cilt: 51 Sayı: 3

Kaynak Göster

APA Ün, Z. P., & Ün, M. (2004). The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51(3), 205-209.
AMA Ün ZP, Ün M. The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes. Ankara Univ Vet Fak Derg. Eylül 2004;51(3):205-209.
Chicago Ün, Zeynep Pınar, ve Mustafa Ün. “The Comparison of the Effectiveness of the Criterions Used to Determine the in Vitro Maturation of Bovine Oocytes”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51, sy. 3 (Eylül 2004): 205-9.
EndNote Ün ZP, Ün M (01 Eylül 2004) The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51 3 205–209.
IEEE Z. P. Ün ve M. Ün, “The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes”, Ankara Univ Vet Fak Derg, c. 51, sy. 3, ss. 205–209, 2004.
ISNAD Ün, Zeynep Pınar - Ün, Mustafa. “The Comparison of the Effectiveness of the Criterions Used to Determine the in Vitro Maturation of Bovine Oocytes”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 51/3 (Eylül 2004), 205-209.
JAMA Ün ZP, Ün M. The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes. Ankara Univ Vet Fak Derg. 2004;51:205–209.
MLA Ün, Zeynep Pınar ve Mustafa Ün. “The Comparison of the Effectiveness of the Criterions Used to Determine the in Vitro Maturation of Bovine Oocytes”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 51, sy. 3, 2004, ss. 205-9.
Vancouver Ün ZP, Ün M. The comparison of the effectiveness of the criterions used to determine the in vitro maturation of bovine oocytes. Ankara Univ Vet Fak Derg. 2004;51(3):205-9.