Ankara Üniversitesi Veteriner Fakültesi Dergisi
Araştırma Makalesi
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Yıl 2019, Cilt: 66 Sayı: 4, 373 - 378, 09.09.2019

Zerrin ERGİNKAYA Hatice ULUDAĞ Emel ÜNAL TURHAN

https://doi.org/10.33988/auvfd.543426

Öz

Kaynakça

  • 1. Achemchem F, Bueno MM, Abrini J et al (2005): Enterococcus faecium F58, a bacteriocinogenic strain naturally occurring in Jben, a soft, farm house goat’s cheese made in Morocco. J Appl Microbiol, 99, 141-15.
  • 2. Anandani JH, Khan ZH (2014): Isolation, partial purification and biochemical characterization of enterocin producing enterococci. Indian J Appl Res, 4, 18-20.
  • 3. Aroujo TF, Ferreira LLF (2013): The genus Enterococcus as probiotic: Safety concerns. Braz Arch Biol Techn, 56, 457-466.
  • 4. Belicová A, Mikulášová M, Krajčovič J et al (2011): Antibacterial activity and enterocin genes in enterococci isolated from Bryndza cheese. J Food Nutr Res, 50, 21–32.
  • 5. Belguesmia Y, Choiset Y, Pr´evost H et al (2010): Partial purification and characterization of the mode of action of enterocin s37:a bacteriocin produced by Enterococcus faecalis S37 isolated from poultry feces. J Environ Pub Health, 986460, 1-8.
  • 6. Cisneros YMA, Espuñes TRS, Wacher C et al (2011): Enterocins: Bacteriocins with Applications in the Food Industry. 1330-1341. In: A. Méndez-Vilas (Ed), Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, Microbiology Series (No 3). Formatex, Badajoz, Spain.
  • 7. De Vuyst L, Morenoa MRF Revets H (2003): Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int J Food Microbiol, 84, 299-318.
  • 8. Franz CMAP, Toit MD, Holy A et al (1997): Production of nisin-like bacteriocins by Lactococcus lactis strains isolated from vegetables. J Basic Microbiol, 37, 187-196.
  • 9. Ghrairi T, Free J, Berjeaud JM et al (2008): Purification and characterization of bacteriocin produced by Enterococcus faecium from Tunisian Rigouta Cheese. Food Control, 19, 162-169.
  • 10. Gómez NC, Abriouel H, Grande MJ et al (2012): Effect of enterocin AS-8 in combination with biocides on planktonic and sessile Listeria monocytogenes. Food Microbiol, 30, 51-58.
  • 11. Isleroglu H, Yildirim Z, Tokatli M (2012): Partial characterization of enterocin KP produced by Enterococcus faecalis KP, a cheese isolate. Int J Dairy Techn, 65, 90-97.
  • 12. Javed A, Masud T, Ain Q et al (2011): Enterocins of Enterococcus faecium, emerging natural food preservatives. Ann Microbiol, 61, 699-708.
  • 13. Javed I, Ali MI, Ahmed B, et al (2011): Optimization and partial purification of bacteriocins from Enterococcus spp. indigenous to Pakistan. Food Biotechn, 25, 130-139.
  • 14. Khalkhali S, Mojgani N (2017): Bacteriocinogenic potential and virulence traits of Enterococcus faecium and E. faecalis isolated from human milk. Iranian J Microbiol, 9, 224-233.
  • 15. Marekova M, Laukova A, De Vuyst L, et al (2003): Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. J Appl Microbiol, 94, 523-530.
  • 16. Moreno MRF, Callewaert R, Devreese B, et al (2003): Isolation and biochemical characterization of enterocins produced by enterococci from different sources. J Applied Microbiol, 94, 214-229.
  • 17. Nascimento MS, Moreno I, Kuaye AY (2010): Antimicrobial activity of Enterococcus faecium fair-E 198 against Gram-Positive pathogens. Braz J Microbiol, 41, 74-81.
  • 18. Ogaki MB, Rocha KR, Terra MR, et al (2016): Screening of the enterocin-encoding genes and antimicrobial activity in Enterococcus species. J Microbiol Biotechn., 26, 1026-1034.
  • 19. Özdemir GB, Oryaşın E, Bıyık HH, et al (2011): Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian J Microbiol, 51, 182-187.
  • 20. Perumal V, Repally A, Dasari A, et al (2016): Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes. Prep Biochem Biotech, 46, 686-694.
  • 21. Savadago A, Ouattara CAT, Bassole IHN (2004): Antimicrobial activities of lactic acid bacteria strains isolated from Burkinofaso fermented milk. Pak J Nutr, 3, 174-179.
  • 22. Sfaxi IH, Ghaish SE, Ahmadova A, et al (2011): Antimicrobial activity and safety of use of Enterococcus faecium PC4.1 isolated from Mongol yogurt. Food Control, 22, 2020-2027.
  • 23. Sivakumar N, Saif R, Saif AB (2010): Partial characterization of bacteriocins produced by Lactobacillus acidophilus and Pediococcus acidilactici. Braz Arch Biol Techn, 53, 1177-1184.
  • 24. Sparo MD, Castro MS, Andino PJ, et al (2006): Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis. J Appl Microbiol, 100, 123-134.
  • 25. Theppangna W, Murase T, Tokumaru N, et al (2007): Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins. J Vet Med Sci, 69, 1235-1239.
  • 26. Toit MD, Franz CMAP, Dicks LMT, et al (2000): Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces. J Appl Microbiol, 88, 482-494.
  • 27. Tulini FL., Gomes BC, Martinis ECP (2011): Partial purifcation and characterization of a bacteriocin produced by Enterococcus faecium 130 isolated from mozzarella cheese. Ciênc Tecnol Aliment, 31, 155-159.
  • 28. Vimont A, Fernandez B, Hammami R, et al (2017): Bacteriocin-producing Enterococcus faecium lcw44: a high potential probiotic candidate from raw camel milk. Front Microbiol, 8, 1-8.
  • 29. Vijayakumar PP, Muriana PM (2017): Inhibition of Listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action. Foods, 6, 1-13.
  • 30. Yildirim Z, İlk Y, Yildirim M, et al (2014): Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk. Turk J Biol, 38, 412-419.

Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria

Yıl 2019, Cilt: 66 Sayı: 4, 373 - 378, 09.09.2019

Zerrin ERGİNKAYA Hatice ULUDAĞ Emel ÜNAL TURHAN

https://doi.org/10.33988/auvfd.543426

Öz

The purpose of the
present research was to obtain enterocins from bacteriocinogenic enterococci (Enterococcus faecalis and Enterococcus faecium) in clinical and
food sources, and to determine antibacterial activity of these enterocins
against pathogenic bacteria including Escherichia
coli, Staphylococcus aureus, Bacillus cereus and Salmonella Enteritidis. Enterocins were partially purified with
ammonium sulfate precipitation from E.
faecium and E. faecalis. After
purification, the antimicrobial activity of enterocin was tested on Mueller
Hinton Agar by disk diffusion assay. The 13, 8, 4 and 1 of 20 bacteriocins
obtained by Enterococcus strain
showed antimicrobial effect against S. Enteritidis,
B. cereus, E. coli and S. aureus,
respectively. One of food origin Enterococcus
(E. faecium) exhibited the
antimicrobial effect on all of the pathogen microorganisms used in our study.
Enterocins from food and clinical isolates were very effective against Salmonella Enteritidis. The most active enterocins were
produced by enterococci isolates from Hatay cow cheese due to their
antibacterial spectrum on pathogenic bacteria used in this study. This study
concluded the importance of investigating clinical enterococci besides
foodborne enterococci to benefit from antibacterial properties.

Anahtar Kelimeler

Antibacterial, bacteriocin, enterocin, enterococci

Kaynakça

  • 1. Achemchem F, Bueno MM, Abrini J et al (2005): Enterococcus faecium F58, a bacteriocinogenic strain naturally occurring in Jben, a soft, farm house goat’s cheese made in Morocco. J Appl Microbiol, 99, 141-15.
  • 2. Anandani JH, Khan ZH (2014): Isolation, partial purification and biochemical characterization of enterocin producing enterococci. Indian J Appl Res, 4, 18-20.
  • 3. Aroujo TF, Ferreira LLF (2013): The genus Enterococcus as probiotic: Safety concerns. Braz Arch Biol Techn, 56, 457-466.
  • 4. Belicová A, Mikulášová M, Krajčovič J et al (2011): Antibacterial activity and enterocin genes in enterococci isolated from Bryndza cheese. J Food Nutr Res, 50, 21–32.
  • 5. Belguesmia Y, Choiset Y, Pr´evost H et al (2010): Partial purification and characterization of the mode of action of enterocin s37:a bacteriocin produced by Enterococcus faecalis S37 isolated from poultry feces. J Environ Pub Health, 986460, 1-8.
  • 6. Cisneros YMA, Espuñes TRS, Wacher C et al (2011): Enterocins: Bacteriocins with Applications in the Food Industry. 1330-1341. In: A. Méndez-Vilas (Ed), Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, Microbiology Series (No 3). Formatex, Badajoz, Spain.
  • 7. De Vuyst L, Morenoa MRF Revets H (2003): Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int J Food Microbiol, 84, 299-318.
  • 8. Franz CMAP, Toit MD, Holy A et al (1997): Production of nisin-like bacteriocins by Lactococcus lactis strains isolated from vegetables. J Basic Microbiol, 37, 187-196.
  • 9. Ghrairi T, Free J, Berjeaud JM et al (2008): Purification and characterization of bacteriocin produced by Enterococcus faecium from Tunisian Rigouta Cheese. Food Control, 19, 162-169.
  • 10. Gómez NC, Abriouel H, Grande MJ et al (2012): Effect of enterocin AS-8 in combination with biocides on planktonic and sessile Listeria monocytogenes. Food Microbiol, 30, 51-58.
  • 11. Isleroglu H, Yildirim Z, Tokatli M (2012): Partial characterization of enterocin KP produced by Enterococcus faecalis KP, a cheese isolate. Int J Dairy Techn, 65, 90-97.
  • 12. Javed A, Masud T, Ain Q et al (2011): Enterocins of Enterococcus faecium, emerging natural food preservatives. Ann Microbiol, 61, 699-708.
  • 13. Javed I, Ali MI, Ahmed B, et al (2011): Optimization and partial purification of bacteriocins from Enterococcus spp. indigenous to Pakistan. Food Biotechn, 25, 130-139.
  • 14. Khalkhali S, Mojgani N (2017): Bacteriocinogenic potential and virulence traits of Enterococcus faecium and E. faecalis isolated from human milk. Iranian J Microbiol, 9, 224-233.
  • 15. Marekova M, Laukova A, De Vuyst L, et al (2003): Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. J Appl Microbiol, 94, 523-530.
  • 16. Moreno MRF, Callewaert R, Devreese B, et al (2003): Isolation and biochemical characterization of enterocins produced by enterococci from different sources. J Applied Microbiol, 94, 214-229.
  • 17. Nascimento MS, Moreno I, Kuaye AY (2010): Antimicrobial activity of Enterococcus faecium fair-E 198 against Gram-Positive pathogens. Braz J Microbiol, 41, 74-81.
  • 18. Ogaki MB, Rocha KR, Terra MR, et al (2016): Screening of the enterocin-encoding genes and antimicrobial activity in Enterococcus species. J Microbiol Biotechn., 26, 1026-1034.
  • 19. Özdemir GB, Oryaşın E, Bıyık HH, et al (2011): Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian J Microbiol, 51, 182-187.
  • 20. Perumal V, Repally A, Dasari A, et al (2016): Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes. Prep Biochem Biotech, 46, 686-694.
  • 21. Savadago A, Ouattara CAT, Bassole IHN (2004): Antimicrobial activities of lactic acid bacteria strains isolated from Burkinofaso fermented milk. Pak J Nutr, 3, 174-179.
  • 22. Sfaxi IH, Ghaish SE, Ahmadova A, et al (2011): Antimicrobial activity and safety of use of Enterococcus faecium PC4.1 isolated from Mongol yogurt. Food Control, 22, 2020-2027.
  • 23. Sivakumar N, Saif R, Saif AB (2010): Partial characterization of bacteriocins produced by Lactobacillus acidophilus and Pediococcus acidilactici. Braz Arch Biol Techn, 53, 1177-1184.
  • 24. Sparo MD, Castro MS, Andino PJ, et al (2006): Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis. J Appl Microbiol, 100, 123-134.
  • 25. Theppangna W, Murase T, Tokumaru N, et al (2007): Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins. J Vet Med Sci, 69, 1235-1239.
  • 26. Toit MD, Franz CMAP, Dicks LMT, et al (2000): Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces. J Appl Microbiol, 88, 482-494.
  • 27. Tulini FL., Gomes BC, Martinis ECP (2011): Partial purifcation and characterization of a bacteriocin produced by Enterococcus faecium 130 isolated from mozzarella cheese. Ciênc Tecnol Aliment, 31, 155-159.
  • 28. Vimont A, Fernandez B, Hammami R, et al (2017): Bacteriocin-producing Enterococcus faecium lcw44: a high potential probiotic candidate from raw camel milk. Front Microbiol, 8, 1-8.
  • 29. Vijayakumar PP, Muriana PM (2017): Inhibition of Listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action. Foods, 6, 1-13.
  • 30. Yildirim Z, İlk Y, Yildirim M, et al (2014): Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk. Turk J Biol, 38, 412-419.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Makaleler
Yazarlar

Zerrin ERGİNKAYA

0000-0001-6208-2927

Hatice ULUDAĞ

0000-0003-2085-8656
Türkiye

Emel ÜNAL TURHAN

0000-0002-0284-574X
Türkiye

Yayımlanma Tarihi 9 Eylül 2019
Yayınlandığı Sayı Yıl 2019Cilt: 66 Sayı: 4

Kaynak Göster

Bibtex @araştırma makalesi { auvfd543426, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2019}, volume = {66}, number = {4}, pages = {373 - 378}, doi = {10.33988/auvfd.543426}, title = {Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria}, key = {cite}, author = {Erginkaya, Zerrin and Uludağ, Hatice and Ünal Turhan, Emel} }
APA Erginkaya, Z. , Uludağ, H. & Ünal Turhan, E. (2019). Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 66 (4) , 373-378 . DOI: 10.33988/auvfd.543426
MLA Erginkaya, Z. , Uludağ, H. , Ünal Turhan, E. "Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 373-378 <http://vetjournal.ankara.edu.tr/tr/pub/issue/47708/543426>
Chicago Erginkaya, Z. , Uludağ, H. , Ünal Turhan, E. "Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 373-378
RIS TY - JOUR T1 - Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria AU - ZerrinErginkaya, HaticeUludağ, EmelÜnal Turhan Y1 - 2019 PY - 2019 N1 - doi: 10.33988/auvfd.543426 DO - 10.33988/auvfd.543426 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 373 EP - 378 VL - 66 IS - 4 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.543426 UR - https://doi.org/10.33988/auvfd.543426 Y2 - 2019 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria %A Zerrin Erginkaya , Hatice Uludağ , Emel Ünal Turhan %T Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria %D 2019 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 66 %N 4 %R doi: 10.33988/auvfd.543426 %U 10.33988/auvfd.543426
ISNAD Erginkaya, Zerrin , Uludağ, Hatice , Ünal Turhan, Emel . "Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 / 4 (Eylül 2019): 373-378 . https://doi.org/10.33988/auvfd.543426
AMA Erginkaya Z. , Uludağ H. , Ünal Turhan E. Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Univ Vet Fak Derg. 2019; 66(4): 373-378.
Vancouver Erginkaya Z. , Uludağ H. , Ünal Turhan E. Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2019; 66(4): 373-378.
IEEE Z. Erginkaya , H. Uludağ ve E. Ünal Turhan , "Antibacterial activity of partially purified enterocins from foodborne and clinical enterococci against some pathogenic bacteria", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 66, sayı. 4, ss. 373-378, Eyl. 2019, doi:10.33988/auvfd.543426