The purpose of
this study was to determine the phenotypic antibiotic susceptibility patterns,
extended-spectrum β-lactamase (ESBL) genes and genotypic profiles of
ESBL-positive Escherichia coli
strains isolated from urine samples obtained from outpatients with urinary
tract infection in Turkey. A total of 120 E.
coli strains during 2017, 2018, and 2019 (40 patients per year) were
examined for antibiotic susceptibility patterns by disc diffusion method, for
ESBL genes using PCR and sequencing and for molecular typing by pulsed-field
gel electrophoresis (PFGE) method. The isolates were evaluated for their
sensitivity to 21 different antibiotics. Four different antimicrobial
resistance patterns were determined according to antibiotic susceptibility
status of the isolates. The β-lactamase genes detected in the isolates were
CTX-M-15 + OXA-1 (n= 14), CTX-M-15 (n= 24), TEM-1 + CTX-M-15 (n= 52), TEM-1 +
SHV-12 (n=6), SHV-12 1 (n= 6), TEM-1 + CTX-M-1 (n= 6), TEM-1 + CTX-M-16 (n= 6)
and TEM-1 + CTX-M-9 (n= 6). The CTX-M-15 was the most prevalent ESBL enzyme in
the isolates. As a result of PFGE analysis performed by XbaI enzyme restriction
process, one major PFGE profile and three main groups (Group I-II-III) were
observed. While antibiotic resistance profiles of the strains showed four
groups (RI-RII-RIII-RIV), PFGE band profiles showed a major group (90%
similarity ratio). High ESBL production and decreased susceptibility to
broad-spectrum cephalosporins were observed in E. coli strains. In addition, PFGE analysis showed high clonal
similarity among E. coli isolates.
Antimicrobial drug resistance ESBL genes Escherichia coli molecular subtyping pulsed-field gel electrophoresis
Birincil Dil | İngilizce |
---|---|
Konular | Veteriner Cerrahi |
Bölüm | Makaleler |
Yazarlar | |
Yayımlanma Tarihi | 9 Eylül 2019 |
Yayımlandığı Sayı | Yıl 2019Cilt: 66 Sayı: 4 |