Infectious pancreatic necrosis virus
(IPNV; Birnaviridae, Aquabirnavirus)
causes infectious pancreatic necrosis (IPN) in fish. IPN disease was first
found in 2002 in Turkey. In this study, 10 IPNV isolates were isolated between
2005 and 2013 and propagated in RTG-2 cell cultures. RNAs obtained from cell lysates were used as template and
VP1, VP2, VP3, VP4 and VP5 genes of the virus were amplified in full
length. Sequence analyses of the genes were made. The obtained sequences were
compared with international reference strains from GenBank and phylogenetic analyses and genogrouping
of the viruses were conducted. Turkish isolates were found to show a
genetic similarity of between 93.5%
and 99.8% in terms of molecules. When segment A sequences were compared with
each other, a similarity between 97.8% and 99.8% was found; when they were compared with international
reference strains, they were found to have the highest similarity (99.3%) with France
AJ622822 isolate and the lowest (65.7%) similarity with Canada NC001915
isolate. When segment B
sequences of the isolates were compared with each other, a similarity between 93.5% and 99.5% was found; when they
were compared with international reference strains, they were found to have the
highest similarity (98.9%) with Canada M58757 isolate and the
lowest (52.7%) similarity with Finland KY548519 strain. As a result of phylogenetic analyses conducted, Turkish isolates were found
to be closely related with France, Spain and Iran strains in serotype A2 and
genogroup 5 they were placed in.
Birincil Dil | İngilizce |
---|---|
Konular | Veteriner Cerrahi |
Bölüm | Makaleler |
Yazarlar | |
Yayımlanma Tarihi | 26 Aralık 2019 |
Yayımlandığı Sayı | Yıl 2020Cilt: 67 Sayı: 1 |