Research Article
BibTex RIS Cite

Year 2020, Volume: 67 Issue: 2, 121 - 125, 03.03.2020

Nazlı Ercan , Murat Yüksel , Mustafa Koçkaya

https://doi.org/10.33988/auvfd.492765

Abstract

References

  • 1. Aebi H (1984): Catalase in vitro. Methods Enzymol, 105, 121-6.
  • 2. Aydın İ, Bülbül A, Avcı GE, et al (2009): Serum oxidative status and adenosine deaminase activity in dogs with transmissible venereal tumour. Bull Vet Inst Pulawy, 53, 771- 774.
  • 3. Baştan A, Baki Acar D, Cengiz M (2008): Uterine and ovarian metastasis of transmissible venereal tumour in a bitch. Turk J Vet Anim Sci, 32, 65-66.
  • 4. Dinçer Y, Akçay T, Kural AR, et al (2011): Evaluation of 8-hydroxy-2'-deoxyguanosine concentration and antioxidant enzyme activities in bladder cancer patients. Turkiye Klinikleri J Med Sci, 31, 553-558.
  • 5. Durak I, Yurtaslanı Z, Canbolat O, et al (1993): A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta, 214,103-104.
  • 6. Ercan N, Fidancı UR (2012): Piyodermalı köpeklerde 8-hidroksi-2’-deoksiguanozin (8-OHdG) düzeyleri. Ankara Üniv Vet Fak Derg, 59, 163-168.
  • 7. Fraga C, Shigenaga M, Park J, et al (1990): Oxidative damage to DNA during aging: 8-Hydroxy-2’-deoxyguanosine in rat organ DNA urine. Proc Natl Acad Sci, 87, 4533-4537.
  • 8. Gencer M, Ceyla E, Aksoy A, et al (2005): Oksidatif stres benign ve malign akciğer hastalıklarının ayırıcı tanısında belirteç olabilir mi? Akciğer Arşivi, 6, 89-92.
  • 9. Haliwell B (2007): Oxidative stress and cancer: have we moved forward? Biochem J, 401, 1-11.
  • 10. Hattori Y, Nishigori C, Tanaka T, et al (1996): 8-hydroxy-2’-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J Invest Dermatol, 107, 733-737.
  • 11. Janero DR (1990): Malondialdehyde and thiobarbituric acid-reactivy as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9, 515-540.
  • 12. Jayasri K, Padmaja K, Saibaba M (2016): Altered oxidative stress and carbohydrate metabolism in canine mammary tumors. Veterinary world, 9, 1489.
  • 13. Kumaraguruparan R, Balachandran C, Manohar BM, et al (2005): Altered oxidant-antioxidant profile in canine mammary tumours, Vet Res Commun, 29, 287-96.
  • 14. Lin H, Jenner A, Ong C, et al (2004): A high-throughput and sensitive methodology for the quantification of urinary 8-hydroxy-2’-deoxyguanosine: measurement with gas chromatography-mass spectrometry after single solid-phase extraction. Biochem J, 380, 541-548.
  • 15. Macotpet A, Suksawat F, Sukon P, et al (2013): Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde. BMC Vet Res, 9, 101.
  • 16. Martinis B, Bianchi M (2002): Methodology for urinary 8-hydroxy-2’-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res, 46, 129-131.
  • 17. Matsufuji H, Ochi H, Shibamoto T (2006): Formation and inhibition of genotoxic malonaldehyde from DNA oxidation controlled with EDTA. Food Chem Toxicol, 44, 236-241.
  • 18. Nak D, Mısırlıoğlu D, Nak Y, et al (2004): Bir köpekte meme metastazlı transmissible venereal tümör olgusu. Vet Bil Derg, 20, 99-102.
  • 19. Paglia DE, WN Valentine (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
  • 20. Pilger A, Ivancsits S, Germadnik D, et al (2002): Urinary excretion of 8-hydroxy-2’-deoxyguanosine measured by high-performance liquid chromatography with electrochemical detection. J Chromatogr B, 778, 393-401.
  • 21. SPSS (2014): IBM SPSS Statistics for Windows, ver¬sion 22.00. SPSS Inc., Chicago, IL.
  • 22. Strakova A, Murchison EP (2014): The changing global distribution and prevalence of canine transmissible venereal tumour. BMC Vet Res, 10, 168.
  • 23. Sun Y, Oberley LW, Li Y (1988): A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
  • 24. Tanja P, Alenka NS, Butinar J, et al (2008): Antioxidant status in canine cancer patients. Acta Vet Beograd, 58, 275-286.
  • 25. Tüközkan N, Erdamar H, Seven I (2006): Measurement of total malondialdehyde in plasma and tissues by high-performance liquid chromatography and thiobarbituric acid assay. Fırat Tıp Derg, 11, 88-92.
  • 26. Urso ML, Clarkson PM (2003): Oxidative stress, exercise and antioxidant supplementation. Toxicology, 189, 41-54.
  • 27. Welsh JS (2011). Contagious Cancer. The Oncologist, 16, 1-4.
  • 28. Winter JL, Barber LG., Freeman L, et al (2009): Antioxidant status and biomarkers of oxidative stress in dogs with Lymphoma. J Vet Intern Med, 23, 311-316.
  • 29. Wiseman H, Kaur H, Halliwell B (1995): DNA damage and cancer: measurement and mechanism. Cancer Letters, 93, 113-120.
  • 30. Wu LL, Chiou CC, Chang PY, et al (2004): Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics Clin Chim Acta, 339, 1-9.
  • 31. Zhang F, Stott WT, Clark AJ, et al (2007): Quantitation of 8-hydroxydeoxyguanosine in DNA by liquid chromatography/positive atmospheric pressure photoionization tandem mass spectrometry. Rapid Commun Mass Spectrom, 21, 3949-3955.

Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour

Year 2020, Volume: 67 Issue: 2, 121 - 125, 03.03.2020

Nazlı Ercan , Murat Yüksel , Mustafa Koçkaya

https://doi.org/10.33988/auvfd.492765

Abstract

Canine transmissible venereal tumour (TVT) is a contagious cancer and sexually transmitted one dog to another by allogenic transfer of living cancer cells. The aim of this study is to examine the relationship between antioxidative metabolism and venereal tumour at Kangal Dogs and to show the size of oxidative damage it causes through 8-hydroxy-2’deoxyguanosine (8-OHdG). The materials of this study included the blood sera of 15 Kangal Dogs with TVT, and as a control group of 15 healthy Kangal Dogs. The sera levels of 8-OHdG were determined by competitive enzyme linked immunosorbent assay (ELISA) method. The level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) which are antioxidative enzymes were investigated by spectrophotometric methods as described. Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P<0.001). The levels of GPx were statistically different (P<0.05). In the light of the findings, which are obtained in this study, the transmissible venereal tumour, which is an infectious and treatable type of cancer, can lead to new studies that may be caused by oxidant-antioxidant metabolism.

Keywords

antioxidant enzymes Kangal dog transmissible venereal tumour 8-OHdG

References

  • 1. Aebi H (1984): Catalase in vitro. Methods Enzymol, 105, 121-6.
  • 2. Aydın İ, Bülbül A, Avcı GE, et al (2009): Serum oxidative status and adenosine deaminase activity in dogs with transmissible venereal tumour. Bull Vet Inst Pulawy, 53, 771- 774.
  • 3. Baştan A, Baki Acar D, Cengiz M (2008): Uterine and ovarian metastasis of transmissible venereal tumour in a bitch. Turk J Vet Anim Sci, 32, 65-66.
  • 4. Dinçer Y, Akçay T, Kural AR, et al (2011): Evaluation of 8-hydroxy-2'-deoxyguanosine concentration and antioxidant enzyme activities in bladder cancer patients. Turkiye Klinikleri J Med Sci, 31, 553-558.
  • 5. Durak I, Yurtaslanı Z, Canbolat O, et al (1993): A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta, 214,103-104.
  • 6. Ercan N, Fidancı UR (2012): Piyodermalı köpeklerde 8-hidroksi-2’-deoksiguanozin (8-OHdG) düzeyleri. Ankara Üniv Vet Fak Derg, 59, 163-168.
  • 7. Fraga C, Shigenaga M, Park J, et al (1990): Oxidative damage to DNA during aging: 8-Hydroxy-2’-deoxyguanosine in rat organ DNA urine. Proc Natl Acad Sci, 87, 4533-4537.
  • 8. Gencer M, Ceyla E, Aksoy A, et al (2005): Oksidatif stres benign ve malign akciğer hastalıklarının ayırıcı tanısında belirteç olabilir mi? Akciğer Arşivi, 6, 89-92.
  • 9. Haliwell B (2007): Oxidative stress and cancer: have we moved forward? Biochem J, 401, 1-11.
  • 10. Hattori Y, Nishigori C, Tanaka T, et al (1996): 8-hydroxy-2’-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J Invest Dermatol, 107, 733-737.
  • 11. Janero DR (1990): Malondialdehyde and thiobarbituric acid-reactivy as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9, 515-540.
  • 12. Jayasri K, Padmaja K, Saibaba M (2016): Altered oxidative stress and carbohydrate metabolism in canine mammary tumors. Veterinary world, 9, 1489.
  • 13. Kumaraguruparan R, Balachandran C, Manohar BM, et al (2005): Altered oxidant-antioxidant profile in canine mammary tumours, Vet Res Commun, 29, 287-96.
  • 14. Lin H, Jenner A, Ong C, et al (2004): A high-throughput and sensitive methodology for the quantification of urinary 8-hydroxy-2’-deoxyguanosine: measurement with gas chromatography-mass spectrometry after single solid-phase extraction. Biochem J, 380, 541-548.
  • 15. Macotpet A, Suksawat F, Sukon P, et al (2013): Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde. BMC Vet Res, 9, 101.
  • 16. Martinis B, Bianchi M (2002): Methodology for urinary 8-hydroxy-2’-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res, 46, 129-131.
  • 17. Matsufuji H, Ochi H, Shibamoto T (2006): Formation and inhibition of genotoxic malonaldehyde from DNA oxidation controlled with EDTA. Food Chem Toxicol, 44, 236-241.
  • 18. Nak D, Mısırlıoğlu D, Nak Y, et al (2004): Bir köpekte meme metastazlı transmissible venereal tümör olgusu. Vet Bil Derg, 20, 99-102.
  • 19. Paglia DE, WN Valentine (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
  • 20. Pilger A, Ivancsits S, Germadnik D, et al (2002): Urinary excretion of 8-hydroxy-2’-deoxyguanosine measured by high-performance liquid chromatography with electrochemical detection. J Chromatogr B, 778, 393-401.
  • 21. SPSS (2014): IBM SPSS Statistics for Windows, ver¬sion 22.00. SPSS Inc., Chicago, IL.
  • 22. Strakova A, Murchison EP (2014): The changing global distribution and prevalence of canine transmissible venereal tumour. BMC Vet Res, 10, 168.
  • 23. Sun Y, Oberley LW, Li Y (1988): A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
  • 24. Tanja P, Alenka NS, Butinar J, et al (2008): Antioxidant status in canine cancer patients. Acta Vet Beograd, 58, 275-286.
  • 25. Tüközkan N, Erdamar H, Seven I (2006): Measurement of total malondialdehyde in plasma and tissues by high-performance liquid chromatography and thiobarbituric acid assay. Fırat Tıp Derg, 11, 88-92.
  • 26. Urso ML, Clarkson PM (2003): Oxidative stress, exercise and antioxidant supplementation. Toxicology, 189, 41-54.
  • 27. Welsh JS (2011). Contagious Cancer. The Oncologist, 16, 1-4.
  • 28. Winter JL, Barber LG., Freeman L, et al (2009): Antioxidant status and biomarkers of oxidative stress in dogs with Lymphoma. J Vet Intern Med, 23, 311-316.
  • 29. Wiseman H, Kaur H, Halliwell B (1995): DNA damage and cancer: measurement and mechanism. Cancer Letters, 93, 113-120.
  • 30. Wu LL, Chiou CC, Chang PY, et al (2004): Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics Clin Chim Acta, 339, 1-9.
  • 31. Zhang F, Stott WT, Clark AJ, et al (2007): Quantitation of 8-hydroxydeoxyguanosine in DNA by liquid chromatography/positive atmospheric pressure photoionization tandem mass spectrometry. Rapid Commun Mass Spectrom, 21, 3949-3955.
There are 31 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Nazlı Ercan 0000-0003-3542-3743

Murat Yüksel 0000-0001-6634-0559

Mustafa Koçkaya 0000-0001-5173-0853

Publication Date March 3, 2020
Published in Issue Year 2020Volume: 67 Issue: 2

Cite

APA Ercan, N., Yüksel, M., & Koçkaya, M. (2020). Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 67(2), 121-125. https://doi.org/10.33988/auvfd.492765
AMA Ercan N, Yüksel M, Koçkaya M. Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Univ Vet Fak Derg. March 2020;67(2):121-125. doi:10.33988/auvfd.492765
Chicago Ercan, Nazlı, Murat Yüksel, and Mustafa Koçkaya. “Determination of 8-Hydroxy-2’deoxyguanosine, Malondialdehyde Levels and Antioxidant Enzyme Activities in Kangal Dogs With Venereal Tumour”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67, no. 2 (March 2020): 121-25. https://doi.org/10.33988/auvfd.492765.
EndNote Ercan N, Yüksel M, Koçkaya M (March 1, 2020) Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 2 121–125.
IEEE N. Ercan, M. Yüksel, and M. Koçkaya, “Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour”, Ankara Univ Vet Fak Derg, vol. 67, no. 2, pp. 121–125, 2020, doi: 10.33988/auvfd.492765.
ISNAD Ercan, Nazlı et al. “Determination of 8-Hydroxy-2’deoxyguanosine, Malondialdehyde Levels and Antioxidant Enzyme Activities in Kangal Dogs With Venereal Tumour”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67/2 (March 2020), 121-125. https://doi.org/10.33988/auvfd.492765.
JAMA Ercan N, Yüksel M, Koçkaya M. Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Univ Vet Fak Derg. 2020;67:121–125.
MLA Ercan, Nazlı et al. “Determination of 8-Hydroxy-2’deoxyguanosine, Malondialdehyde Levels and Antioxidant Enzyme Activities in Kangal Dogs With Venereal Tumour”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 2, 2020, pp. 121-5, doi:10.33988/auvfd.492765.
Vancouver Ercan N, Yüksel M, Koçkaya M. Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Univ Vet Fak Derg. 2020;67(2):121-5.