Ankara Üniversitesi Veteriner Fakültesi Dergisi
Araştırma Makalesi
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Yıl 2020, Cilt: 67 Sayı: 2, 193 - 199, 03.03.2020

Ali Reha AĞAOĞLU Özgecan KORKMAZ AĞAOĞLU Özge SİDEKLİ Mesih KOCAMÜFTÜOĞLU

https://doi.org/10.33988/auvfd.640288

Öz

Kaynakça

  • 1. Adnane M, Kaidi R, Hanzen C, et al (2017): Risk factors of clinical and subclinical endometritis in cattle: a review. Turk J Vet Anim Sci, 41, 1-11.
  • 2. Agaoglu AR, Agaoglu OK, Aslan S, et al (2020): The Effects of Presynch-10 and Ovsynch on Some Endometrial Toll and Nod-like Receptor Gene Expressions in Repeat Breeder Cows. Kafkas Univ Vet Fak Derg, 26, 111-120.
  • 3. Albihn A, Gustafsson H, Hurst M, et al (1991): Embryonic ability to prolong the interestrous interval in virgin and repeat breading heifers. Anim Reprod Sci, 26, 193-210 . 4. Albihn A, Gustafsson H, Rodriguez-Martines H, et al (1989): Development of day 7 bovine demi embryos transferred into virgin and repeat breeder heifers. Anim Reprod Sci, 21, 161-176.
  • 5. Bitar MS (2000): Insulin and glucocorticoid-dependent suppression of the IGF-I system in diabetic wounds. Surgery, 127, 687–695.
  • 6. Burns PD, Spitzer JC, Henricks DM (1997): Effect of dietary energy restriction on follicular development and luteal function in nonlactating beef cows. J Anim Sci, 75, 1078-1086.
  • 7. Geisert RD, Lee CY, Simmen FA, et al (1991): Expression of messenger RNAs encoding insulin-like growth factor-I, -II, and insulin-like growth factor binding protein-2 in bovine endometrium during the estrous cycle and early pregnancy. Biol Reprod, 45, 975–983.
  • 8. Ghahary A, Chakrabarti S, Murphy LJ (1990): In situ localization of the sites of synthesis and action of insulin-like growth factor-1 in the rat uterus. Mol Endocrinol, 4, 191–195.
  • 9. Giudice LA, Dsupin BA, Jin IH, et al (1993): Differential expression of messenger ribonucleic acids encoding insulin-like growth factors and their receptors in human uterine endometrium and decidua. J Clin Endocrinol Metab, 76, 1115–1122.
  • 10. Grazul-Bilska AT, Johnson ML, Bilski JJ, et al (2003): Wound healing: the role of growth factors. Drugs Today, 39, 787–800.
  • 11. Gustafsson Hi Larsson K, Kindahl H, et al (1986): Sequential endocrine changes and behaviour during estrus and metestrus in repeat breeder and virgin heifers. Anim Reprod Sci, 19, 261-273.
  • 12. Janowski T, Barański W, Łukasik K, et al (2013): Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis. Pol J Vet Sci, 16, 693-699.
  • 13. Kasimanicam R, Kasimanicam V, Kastelic JP (2014): Mucin 1 and cytokines mRNA in endometrium of dairy cows with postpartum uterine disease or repeat breeding. Theriogenology, 81, 952-958.
  • 14. Khair A, Asuduzzaman M, Sultana Z, et al (2018): Economic benefit in repeat breeder cows using intrauterine infusion of penicillin and estrus synchronization followed by timed artificial insemination. J Adv Vet Anim Res, 5, 454–458.
  • 15. LeBlanc SJ, Duffied TF, Leslie KE, et al (2002): Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J Dairy Sci, 85, 2223-2236.
  • 16. Livak KJ, Schmittgen TD (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆T method. Methods, 26, 402-408.
  • 17. Llewellyn S, Fitzpatrick R, Kenny DA, et al (2008): Endometrial expression of the insulin-like growth factor system during uterine involution in the postpartum dairy cow. Dom Anim Endocrinol, 34, 391–402.
  • 18. Lucy MC, Bilby CR, Kirby CJ, et al (1999): Role of growth hormone in development and maintenance of follicles and corpora lutea. J Reprod Fertil, 54, 49–59.
  • 19. Mandefro M, Negash G (2014): Repeat Breder Syndrome in Dairy Cows: Influence of Breed and Age on Its Prevalence. World J of Agric Sci, 10, 200-203.
  • 20. Mann GE, Green MP, Sinclair KD, et al (2003): Effects of circulating progesterone and insulin on early embryo development in beef heifers. Anim Reprod Sci, 79, 71–9.
  • 21. Mann GE, Lamming GE (2001): Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction, 121, 175–80.
  • 22. Neuvians TP, Pfaffl MW, Berisha B, et al (2003): The mRNA express, one of the members of the IGF system in bovine corpus luteum during induced luteolysis. Dom Anim Endocrinol, 25, 359-372.
  • 23. Parkinson TJ (2009): The repeat breeder syndrome. 463-466. In: DE Noakes, TJ Parkinson, GCW England, (Eds), Veterinary reproduction and obstetrics. 9th ed., Saunders Elsevier, Edinburgh.
  • 24. Pothmann H, Prunner I, Wagener K, et al (2015): The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows. Theriogenology, 83, 1249-1253.
  • 25. Pursley JR, Mee MO, Wiltbank MC (1995): Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 52, 1067-1078.
  • 26. Radcliff RP, McCormack BL, Crooker BA, et al (2003): Plasma hormones and expression of growth hormone receptor and insulin-like growth factor-I mRNA in hepatic tissue of periparturient dairy cows. J Dairy Sci, 86, 3920-3926.
  • 27. Robinson RS, Mann GE, Gadd TS, et al (2000): The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancy. J Endocrinol, 165, 231-243.
  • 28. Salasel B, Mokhtari A, Taktaz T (2010): Prevalence, risk factors for and impact of subclinical endometritis in repeat breeder dairy cows. Theriogenology, 74, 1271-1278.
  • 29. Simmen RCM, Simmen FA, Hofig A, et al (1990). Hormonal regulation of insulin like growth factor gene expression in pig uterus. Endocrinology, 127, 2166-2174.
  • 30. Souza AH, Gümen A, Silva EPB, et al (2007) Supplementation with estradiol-17  before the last gonadotropin-releasing hormone injection of the ovsynch protocol in lactating cows. J Dairy Sci, 90, 4623-4634.
  • 31. Spicer LJ, Echternkamp SE (1995): The ovarian insulin and insulin-like growth factor system with an emphasis on domestic animals. Dom Anim Endocrinol, 12, 223–245.
  • 32. Stevenson KR, Gilmour RS, Wathes DC (1994): Localization of insulin-like growth factor-I (IGF-I) and -II messenger ribonucleic acid and type 1 IGF receptor in the ovine uterus during the oestrous cycle and early pregnancy. Endocrinology, 134, 1655-1664.
  • 33. Totey SM, Pawshe CH, Rao KBC (1996): In vitro maturation of buffalo oocytes: Role of insulin and its inter- action with gonadotrophins. J Reprod Fertil, 50, 113-119.
  • 34. Valdmann M, Kurykin J, Kaart T, et al (2018): Relationships between plasma insulin like growth factor-1 and insülin concentrations in multiparous dairy cows with cytological endometritis. Vet Rec, 183, 1-7.
  • 35. Wathes DC, Cheng Z, Fenwick MA, et al (2011): Influence of energy balance on the somatotrophic axis and matrix metalloproteinase expression in the endometrium of the postpartum dairy cow. Reproduction, 141, 269-281.
  • 36. Wodaje HB, Mekuria TA (2016): Risk factors of repeat breeding in dairy cattle. Adv Biol Res, 10, 213-221.
  • 37. YoshidaY, Miyamura M, Hamano S, et al (1998): Expression of growth factor ligand and their receptor mRNAs in bovine ova during in vitro maturation and after fertilization in vitro. J Vet Med Sci, 60, 549-554.
  • 38. Yusuf M, Nakao T, Ranasinghe RB, et al (2010): Reproductive performance of repeat breeders in dairy herds. Theriogenology, 73, 1220-1229.
  • 39. Zobel R, Tkaltcic S, Buic V, et al (2011): Repeat breeder syndrome in dairy cows: influence of breed and age on its prevalence and the success of a hormone therapy. Turk J Vet Anim Sci, 35, 405-411.

Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol

Yıl 2020, Cilt: 67 Sayı: 2, 193 - 199, 03.03.2020

Ali Reha AĞAOĞLU Özgecan KORKMAZ AĞAOĞLU Özge SİDEKLİ Mesih KOCAMÜFTÜOĞLU

https://doi.org/10.33988/auvfd.640288

Öz

The aim of this study was to investigate the effects of using presynch-10 and ovsynch synchronization protocols on endometrial expression and serum levels of insulin-like growth factor-1 (IGF-1) that thought to play roles during endometrial healing process in cows diagnosed with repeat breeder syndrome (RBS) and subclinical endometritis (SE). A total of 40 Holstein-Friesian cows identified as having RBS and SE were used. The animals in Group 1 (G1, n=20) were applied presynch-10 and ovsynch furthermore, biopsy and blood samples were collected both before and after administration. Biopsy and blood samples were taken from the animals in Group 2 (G2, n=20) without performing any synchronization. The IGF-1 gene expression profiles were determined in all samples using RT-qPCR. Even though the level of IGF-1 expression increased in samples taken after synchronization in G1, it was not statistically significant (P>0.05). There was no statistical difference in serum IGF-1 levels between G1 and G2 (P>0.05). In conclusion, the expression of the IGF-1 gene in the endometrial tissue of RBS cows with SE was identified, but the evidence suggests that the synchronization protocols in question had no significant effect on the gene expression profile being investigated. 

Anahtar Kelimeler

gene expression, IGF-1, ovsynch, presynch-10, repeat breeder

Kaynakça

  • 1. Adnane M, Kaidi R, Hanzen C, et al (2017): Risk factors of clinical and subclinical endometritis in cattle: a review. Turk J Vet Anim Sci, 41, 1-11.
  • 2. Agaoglu AR, Agaoglu OK, Aslan S, et al (2020): The Effects of Presynch-10 and Ovsynch on Some Endometrial Toll and Nod-like Receptor Gene Expressions in Repeat Breeder Cows. Kafkas Univ Vet Fak Derg, 26, 111-120.
  • 3. Albihn A, Gustafsson H, Hurst M, et al (1991): Embryonic ability to prolong the interestrous interval in virgin and repeat breading heifers. Anim Reprod Sci, 26, 193-210 . 4. Albihn A, Gustafsson H, Rodriguez-Martines H, et al (1989): Development of day 7 bovine demi embryos transferred into virgin and repeat breeder heifers. Anim Reprod Sci, 21, 161-176.
  • 5. Bitar MS (2000): Insulin and glucocorticoid-dependent suppression of the IGF-I system in diabetic wounds. Surgery, 127, 687–695.
  • 6. Burns PD, Spitzer JC, Henricks DM (1997): Effect of dietary energy restriction on follicular development and luteal function in nonlactating beef cows. J Anim Sci, 75, 1078-1086.
  • 7. Geisert RD, Lee CY, Simmen FA, et al (1991): Expression of messenger RNAs encoding insulin-like growth factor-I, -II, and insulin-like growth factor binding protein-2 in bovine endometrium during the estrous cycle and early pregnancy. Biol Reprod, 45, 975–983.
  • 8. Ghahary A, Chakrabarti S, Murphy LJ (1990): In situ localization of the sites of synthesis and action of insulin-like growth factor-1 in the rat uterus. Mol Endocrinol, 4, 191–195.
  • 9. Giudice LA, Dsupin BA, Jin IH, et al (1993): Differential expression of messenger ribonucleic acids encoding insulin-like growth factors and their receptors in human uterine endometrium and decidua. J Clin Endocrinol Metab, 76, 1115–1122.
  • 10. Grazul-Bilska AT, Johnson ML, Bilski JJ, et al (2003): Wound healing: the role of growth factors. Drugs Today, 39, 787–800.
  • 11. Gustafsson Hi Larsson K, Kindahl H, et al (1986): Sequential endocrine changes and behaviour during estrus and metestrus in repeat breeder and virgin heifers. Anim Reprod Sci, 19, 261-273.
  • 12. Janowski T, Barański W, Łukasik K, et al (2013): Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis. Pol J Vet Sci, 16, 693-699.
  • 13. Kasimanicam R, Kasimanicam V, Kastelic JP (2014): Mucin 1 and cytokines mRNA in endometrium of dairy cows with postpartum uterine disease or repeat breeding. Theriogenology, 81, 952-958.
  • 14. Khair A, Asuduzzaman M, Sultana Z, et al (2018): Economic benefit in repeat breeder cows using intrauterine infusion of penicillin and estrus synchronization followed by timed artificial insemination. J Adv Vet Anim Res, 5, 454–458.
  • 15. LeBlanc SJ, Duffied TF, Leslie KE, et al (2002): Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J Dairy Sci, 85, 2223-2236.
  • 16. Livak KJ, Schmittgen TD (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆T method. Methods, 26, 402-408.
  • 17. Llewellyn S, Fitzpatrick R, Kenny DA, et al (2008): Endometrial expression of the insulin-like growth factor system during uterine involution in the postpartum dairy cow. Dom Anim Endocrinol, 34, 391–402.
  • 18. Lucy MC, Bilby CR, Kirby CJ, et al (1999): Role of growth hormone in development and maintenance of follicles and corpora lutea. J Reprod Fertil, 54, 49–59.
  • 19. Mandefro M, Negash G (2014): Repeat Breder Syndrome in Dairy Cows: Influence of Breed and Age on Its Prevalence. World J of Agric Sci, 10, 200-203.
  • 20. Mann GE, Green MP, Sinclair KD, et al (2003): Effects of circulating progesterone and insulin on early embryo development in beef heifers. Anim Reprod Sci, 79, 71–9.
  • 21. Mann GE, Lamming GE (2001): Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction, 121, 175–80.
  • 22. Neuvians TP, Pfaffl MW, Berisha B, et al (2003): The mRNA express, one of the members of the IGF system in bovine corpus luteum during induced luteolysis. Dom Anim Endocrinol, 25, 359-372.
  • 23. Parkinson TJ (2009): The repeat breeder syndrome. 463-466. In: DE Noakes, TJ Parkinson, GCW England, (Eds), Veterinary reproduction and obstetrics. 9th ed., Saunders Elsevier, Edinburgh.
  • 24. Pothmann H, Prunner I, Wagener K, et al (2015): The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows. Theriogenology, 83, 1249-1253.
  • 25. Pursley JR, Mee MO, Wiltbank MC (1995): Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 52, 1067-1078.
  • 26. Radcliff RP, McCormack BL, Crooker BA, et al (2003): Plasma hormones and expression of growth hormone receptor and insulin-like growth factor-I mRNA in hepatic tissue of periparturient dairy cows. J Dairy Sci, 86, 3920-3926.
  • 27. Robinson RS, Mann GE, Gadd TS, et al (2000): The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancy. J Endocrinol, 165, 231-243.
  • 28. Salasel B, Mokhtari A, Taktaz T (2010): Prevalence, risk factors for and impact of subclinical endometritis in repeat breeder dairy cows. Theriogenology, 74, 1271-1278.
  • 29. Simmen RCM, Simmen FA, Hofig A, et al (1990). Hormonal regulation of insulin like growth factor gene expression in pig uterus. Endocrinology, 127, 2166-2174.
  • 30. Souza AH, Gümen A, Silva EPB, et al (2007) Supplementation with estradiol-17  before the last gonadotropin-releasing hormone injection of the ovsynch protocol in lactating cows. J Dairy Sci, 90, 4623-4634.
  • 31. Spicer LJ, Echternkamp SE (1995): The ovarian insulin and insulin-like growth factor system with an emphasis on domestic animals. Dom Anim Endocrinol, 12, 223–245.
  • 32. Stevenson KR, Gilmour RS, Wathes DC (1994): Localization of insulin-like growth factor-I (IGF-I) and -II messenger ribonucleic acid and type 1 IGF receptor in the ovine uterus during the oestrous cycle and early pregnancy. Endocrinology, 134, 1655-1664.
  • 33. Totey SM, Pawshe CH, Rao KBC (1996): In vitro maturation of buffalo oocytes: Role of insulin and its inter- action with gonadotrophins. J Reprod Fertil, 50, 113-119.
  • 34. Valdmann M, Kurykin J, Kaart T, et al (2018): Relationships between plasma insulin like growth factor-1 and insülin concentrations in multiparous dairy cows with cytological endometritis. Vet Rec, 183, 1-7.
  • 35. Wathes DC, Cheng Z, Fenwick MA, et al (2011): Influence of energy balance on the somatotrophic axis and matrix metalloproteinase expression in the endometrium of the postpartum dairy cow. Reproduction, 141, 269-281.
  • 36. Wodaje HB, Mekuria TA (2016): Risk factors of repeat breeding in dairy cattle. Adv Biol Res, 10, 213-221.
  • 37. YoshidaY, Miyamura M, Hamano S, et al (1998): Expression of growth factor ligand and their receptor mRNAs in bovine ova during in vitro maturation and after fertilization in vitro. J Vet Med Sci, 60, 549-554.
  • 38. Yusuf M, Nakao T, Ranasinghe RB, et al (2010): Reproductive performance of repeat breeders in dairy herds. Theriogenology, 73, 1220-1229.
  • 39. Zobel R, Tkaltcic S, Buic V, et al (2011): Repeat breeder syndrome in dairy cows: influence of breed and age on its prevalence and the success of a hormone therapy. Turk J Vet Anim Sci, 35, 405-411.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Araştırma Makalesi
Yazarlar

Ali Reha AĞAOĞLU
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology
0000-0002-6545-8800
Türkiye

Özgecan KORKMAZ AĞAOĞLU
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Animal Science
0000-0002-7414-1725
Türkiye

Özge SİDEKLİ
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Animal Science
0000-0002-4891-1968
Türkiye

Mesih KOCAMÜFTÜOĞLU
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology
0000-0003-3228-6835
Türkiye

Yayımlanma Tarihi 3 Mart 2020
Yayınlandığı Sayı Yıl 2020Cilt: 67 Sayı: 2

Kaynak Göster

Bibtex @araştırma makalesi { auvfd640288, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2020}, volume = {67}, number = {2}, pages = {193 - 199}, doi = {10.33988/auvfd.640288}, title = {Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol}, key = {cite}, author = {Ağaoğlu, Ali Reha and Korkmaz Ağaoğlu, Özgecan and Sidekli, Özge and Kocamüftüoğlu, Mesih} }
APA Ağaoğlu, A. R. , Korkmaz Ağaoğlu, Ö. , Sidekli, Ö. & Kocamüftüoğlu, M. (2020). Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 67 (2) , 193-199 . DOI: 10.33988/auvfd.640288
MLA Ağaoğlu, A. R. , Korkmaz Ağaoğlu, Ö. , Sidekli, Ö. , Kocamüftüoğlu, M. "Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 193-199 <http://vetjournal.ankara.edu.tr/tr/pub/issue/52756/640288>
Chicago Ağaoğlu, A. R. , Korkmaz Ağaoğlu, Ö. , Sidekli, Ö. , Kocamüftüoğlu, M. "Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (2020 ): 193-199
RIS TY - JOUR T1 - Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol AU - Ali RehaAğaoğlu, ÖzgecanKorkmaz Ağaoğlu, ÖzgeSidekli, MesihKocamüftüoğlu Y1 - 2020 PY - 2020 N1 - doi: 10.33988/auvfd.640288 DO - 10.33988/auvfd.640288 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 193 EP - 199 VL - 67 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.640288 UR - https://doi.org/10.33988/auvfd.640288 Y2 - 2019 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol %A Ali Reha Ağaoğlu , Özgecan Korkmaz Ağaoğlu , Özge Sidekli , Mesih Kocamüftüoğlu %T Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol %D 2020 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 67 %N 2 %R doi: 10.33988/auvfd.640288 %U 10.33988/auvfd.640288
ISNAD Ağaoğlu, Ali Reha , Korkmaz Ağaoğlu, Özgecan , Sidekli, Özge , Kocamüftüoğlu, Mesih . "Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 / 2 (Mart 2020): 193-199 . https://doi.org/10.33988/auvfd.640288
AMA Ağaoğlu A. R. , Korkmaz Ağaoğlu Ö. , Sidekli Ö. , Kocamüftüoğlu M. Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol. Ankara Univ Vet Fak Derg. 2020; 67(2): 193-199.
Vancouver Ağaoğlu A. R. , Korkmaz Ağaoğlu Ö. , Sidekli Ö. , Kocamüftüoğlu M. Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2020; 67(2): 193-199.
IEEE A. R. Ağaoğlu , Ö. Korkmaz Ağaoğlu , Ö. Sidekli ve M. Kocamüftüoğlu , "Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 67, sayı. 2, ss. 193-199, Mar. 2020, doi:10.33988/auvfd.640288