Yıl 2021, Cilt 68 , Sayı 2, Sayfalar 113 - 120 2021-03-31

Salmonella spp. is the most frequently isolated foodborne pathogens causing human and animal diseases. The aim of this study was to investigate antimicrobial susceptibility profiles and the molecular typing of 200 Salmonella Enteritidis strains isolated from the patients’ stools between 2016 and 2019 in Turkey. The isolates were examined for antibiotic susceptibility patterns (21 antimicrobial agents) by Kirby-Bauer disc diffusion method or molecular typing by Pulsed-field gel electrophoresis (PFGE) and plasmid profiling. Although all isolates were susceptible to four antibiotics (suphamethoxazole/trimethoprim, chloramphenicol, streptomycin and trimethoprim), all were resistant to 15 different antibiotics. In the PFGE study performed with XbaI enzyme, all isolates were found to be related to each other according to similarity rates of 85% and above. There were two major clones, clone A and B. Clone A was divided into 6 pulsotypes (A1-A2-A3-A4-A5-A6) and clone B was divided into 3 pulsotypes (B1-B2-B3). Clone A had 87% similarity and Clone B had 90% similarity. The clustering rate was 86% (172/200). All isolates harboured 1-4 plasmid ranging in size from 2.5 to 57 kb and showed 6 plasmid profiles (P1-P6). All isolates carried the 57 kb plasmid individually or in combination with other plasmids. Most of the isolates 136 (68%) had P2 profile. Our findings indicate that the majority of all isolates were clonally related and had cross contamination problems. In this study, the importance of molecular typing methods in order to take more effective protection and control measures against Salmonella has been demonstrated and proposed to use such methods.
Antimicrobial resistance, PFGE, plasmid profiling, Salmonella Enteritidis
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Birincil Dil en
Konular Veteriner Hekimlik
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-8178-223X
Yazar: Alper KARAGÖZ
Kurum: Uşak University, Department of Molecular Biology and Genetics
Ülke: Turkey


Orcid: 0000-0002-5148-723X
Yazar: Levent ALTINTAŞ
Kurum: Ankara University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology
Ülke: Turkey


Orcid: 0000-0002-4351-6600
Yazar: Tutku ARSLANTAŞ
Kurum: Çankırı Public Hospital, Microbiology Laboratory
Ülke: Turkey


Orcid: 0000-0001-9512-8637
Yazar: Hidayet TUTUN (Sorumlu Yazar)
Kurum: Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology
Ülke: Turkey


Orcid: 0000-0002-1727-1582
Yazar: Nadir KOÇAK
Kurum: Selçuk University, Medical Genetics
Ülke: Turkey


Orcid: 0000-0001-6467-9647
Yazar: Özlem ALTINTAŞ
Kurum: Veterinary Control Central Research Institute
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 31 Mart 2021

Bibtex @araştırma makalesi { auvfd691746, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2021}, volume = {68}, pages = {113 - 120}, doi = {10.33988/auvfd.691746}, title = {Phenotypic and molecular characterization of Salmonella Enteritidis isolates}, key = {cite}, author = {Karagöz, Alper and Altıntaş, Levent and Arslantaş, Tutku and Tutun, Hidayet and Koçak, Nadir and Altıntaş, Özlem} }
APA Karagöz, A , Altıntaş, L , Arslantaş, T , Tutun, H , Koçak, N , Altıntaş, Ö . (2021). Phenotypic and molecular characterization of Salmonella Enteritidis isolates . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 68 (2) , 113-120 . DOI: 10.33988/auvfd.691746
MLA Karagöz, A , Altıntaş, L , Arslantaş, T , Tutun, H , Koçak, N , Altıntaş, Ö . "Phenotypic and molecular characterization of Salmonella Enteritidis isolates" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 (2021 ): 113-120 <http://vetjournal.ankara.edu.tr/tr/pub/issue/60673/691746>
Chicago Karagöz, A , Altıntaş, L , Arslantaş, T , Tutun, H , Koçak, N , Altıntaş, Ö . "Phenotypic and molecular characterization of Salmonella Enteritidis isolates". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 (2021 ): 113-120
RIS TY - JOUR T1 - Phenotypic and molecular characterization of Salmonella Enteritidis isolates AU - Alper Karagöz , Levent Altıntaş , Tutku Arslantaş , Hidayet Tutun , Nadir Koçak , Özlem Altıntaş Y1 - 2021 PY - 2021 N1 - doi: 10.33988/auvfd.691746 DO - 10.33988/auvfd.691746 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 113 EP - 120 VL - 68 IS - 2 SN - -1308-2817 M3 - doi: 10.33988/auvfd.691746 UR - https://doi.org/10.33988/auvfd.691746 Y2 - 2020 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Phenotypic and molecular characterization of Salmonella Enteritidis isolates %A Alper Karagöz , Levent Altıntaş , Tutku Arslantaş , Hidayet Tutun , Nadir Koçak , Özlem Altıntaş %T Phenotypic and molecular characterization of Salmonella Enteritidis isolates %D 2021 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 68 %N 2 %R doi: 10.33988/auvfd.691746 %U 10.33988/auvfd.691746
ISNAD Karagöz, Alper , Altıntaş, Levent , Arslantaş, Tutku , Tutun, Hidayet , Koçak, Nadir , Altıntaş, Özlem . "Phenotypic and molecular characterization of Salmonella Enteritidis isolates". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 / 2 (Mart 2021): 113-120 . https://doi.org/10.33988/auvfd.691746
AMA Karagöz A , Altıntaş L , Arslantaş T , Tutun H , Koçak N , Altıntaş Ö . Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Univ Vet Fak Derg. 2021; 68(2): 113-120.
Vancouver Karagöz A , Altıntaş L , Arslantaş T , Tutun H , Koçak N , Altıntaş Ö . Phenotypic and molecular characterization of Salmonella Enteritidis isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 113-120.
IEEE A. Karagöz , L. Altıntaş , T. Arslantaş , H. Tutun , N. Koçak ve Ö. Altıntaş , "Phenotypic and molecular characterization of Salmonella Enteritidis isolates", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 68, sayı. 2, ss. 113-120, Mar. 2021, doi:10.33988/auvfd.691746