Amniotic membrane derived stem cells (AMSCs) are reported to have a comparatively higher potency than multipotent stem cells. These cells are shown to have low immunogenicity and no teratogenicity. Among various conventional methods of isolation using enzymes, explant culture method is believed to be an easy and cost-effective way to harvest stem cells. The purpose of this study was to isolate AMSCs from amniotic membrane of rats and to characterize them for multilineage differentiation, including generation of neurospheres to use them later in in-vivo experiments. Amniotic membranes were collected from Wistar rats on 17th day of pregnancy. After processing of the tissues, AMSCs were isolated by the explant culture method and continued to grow until 10th passage. The doubling time was estimated and the cells were analyzed for growth curve parameters at passages 5 and 9. The osteogenic and adipogenic differentiation studies were carried out from the same cells after 3rd passage. Neurospheres generation from AMSCs was performed using neurogenic induction media. The cells were further assessed for their mesenchymal, haemopoietic, and neurogenic marker expressions by immunofluorescence staining and PCR analysis The study suggests that AMSCs isolated through explant culture are reliable stem cells which could generate neurospheres under proper induction conditions and could be a potential candidate to be used on in-vivo neural degeneration models.
Amniotic membrane, differentiation, explant culture, neurosphere
This work was supported by Afyon Kocatepe University, Scientific Research Projects Coordination Unit (16.KARİYER.23), Afyonkarahisar, Turkey.
We would like to acknowledge Mr. Tayfun Dikmen for his contributions in editing this article. A part of this study was presented in 3rd International Vetistanbul Group Congress, May 17-20 2016, Sarajevo, Bosnia and Herzegovina.
Birincil Dil | İngilizce |
---|---|
Konular | Veteriner Cerrahi |
Bölüm | Araştırma Makalesi |
Yazarlar |
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Proje Numarası | 16.KARİYER.23 |
Yayımlanma Tarihi | 25 Mart 2022 |
Yayınlandığı Sayı | Yıl 2022Cilt: 69 Sayı: 2 |
Bibtex | @araştırma makalesi { auvfd830460, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2022}, volume = {69}, number = {2}, pages = {191 - 199}, doi = {10.33988/auvfd.830460}, title = {Explant culture and multilineage differentiation of amniotic membrane derived stem cells}, key = {cite}, author = {Özden Akkaya, Özlem and Kıbrıa, Asm Golam and Yağcı, Artay and Erdoğan, Metin and Altunbaş, Korhan} } |
APA | Özden Akkaya, Ö. , Kıbrıa, A. G. , Yağcı, A. , Erdoğan, M. & Altunbaş, K. (2022). Explant culture and multilineage differentiation of amniotic membrane derived stem cells . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (2) , 191-199 . DOI: 10.33988/auvfd.830460 |
MLA | Özden Akkaya, Ö. , Kıbrıa, A. G. , Yağcı, A. , Erdoğan, M. , Altunbaş, K. "Explant culture and multilineage differentiation of amniotic membrane derived stem cells" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 191-199 <http://vetjournal.ankara.edu.tr/tr/pub/issue/69048/830460> |
Chicago | Özden Akkaya, Ö. , Kıbrıa, A. G. , Yağcı, A. , Erdoğan, M. , Altunbaş, K. "Explant culture and multilineage differentiation of amniotic membrane derived stem cells". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 191-199 |
RIS | TY - JOUR T1 - Explant culture and multilineage differentiation of amniotic membrane derived stem cells AU - ÖzlemÖzden Akkaya, Asm GolamKıbrıa, ArtayYağcı, MetinErdoğan, KorhanAltunbaş Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.830460 DO - 10.33988/auvfd.830460 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 191 EP - 199 VL - 69 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.830460 UR - https://doi.org/10.33988/auvfd.830460 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Explant culture and multilineage differentiation of amniotic membrane derived stem cells %A Özlem Özden Akkaya , Asm Golam Kıbrıa , Artay Yağcı , Metin Erdoğan , Korhan Altunbaş %T Explant culture and multilineage differentiation of amniotic membrane derived stem cells %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 2 %R doi: 10.33988/auvfd.830460 %U 10.33988/auvfd.830460 |
ISNAD | Özden Akkaya, Özlem , Kıbrıa, Asm Golam , Yağcı, Artay , Erdoğan, Metin , Altunbaş, Korhan . "Explant culture and multilineage differentiation of amniotic membrane derived stem cells". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 2 (Mart 2022): 191-199 . https://doi.org/10.33988/auvfd.830460 |
AMA | Özden Akkaya Ö. , Kıbrıa A. G. , Yağcı A. , Erdoğan M. , Altunbaş K. Explant culture and multilineage differentiation of amniotic membrane derived stem cells. Ankara Univ Vet Fak Derg. 2022; 69(2): 191-199. |
Vancouver | Özden Akkaya Ö. , Kıbrıa A. G. , Yağcı A. , Erdoğan M. , Altunbaş K. Explant culture and multilineage differentiation of amniotic membrane derived stem cells. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 191-199. |
IEEE | Ö. Özden Akkaya , A. G. Kıbrıa , A. Yağcı , M. Erdoğan ve K. Altunbaş , "Explant culture and multilineage differentiation of amniotic membrane derived stem cells", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 69, sayı. 2, ss. 191-199, Mar. 2022, doi:10.33988/auvfd.830460 |