The canine distemper virus (CDV), which infects dogs and a broad range of animal species, remains a serious concern in Türkiye and across the world. The current study shows that CDV can be detected and isolated rapidly and specifically in naturally infected dogs. Whole blood, nasal swab, ocular swab, rectal swab, and urine samples from 50 stray dogs were used in the study (n = 250). The presence of the CDV genome was confirmed in 105 (42%) samples using one-Step real-time RT-PCR. In total, 39 dogs were diagnosed with CDV infection based on the detection of cytopathic effects in MDCK, which was verified by the fluorescent antibody technique. A total of 12 one-Step real-time RT-PCR negative samples, consisting of 4 rectal swabs and 8 urine samples, were found to be positive by virus isolation. Blood, nasal swab, ocular swab (P<0.01, r = 1), rectal swab (P<0.01, r = 0.844), and urine samples (P<0.01, r = 0.697) all showed positive correlations in the tests for viral genome detection and virus isolation. CPE levels of high 37 (31.62%), medium 26 (22.23%) and low 54 (46.15%) were detected in a total of 117 (46.8%) samples with viral growth in cell culture. The highest CPE levels detected by FAT were for rectal swab and urine samples. In conclusion, the one-step real-time RT-PCR method on rectal swab samples proved to be a very sensitive method for the rapid and reliable CDV detection. Besides, non-modified MDCK can be used to isolate CDV from naturally infected dogs.
Canine, cell culture, distemper, fluorescent antibody, TaqMan
Birincil Dil | İngilizce |
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Konular | Veteriner Hekimlik |
Bölüm | Araştırma Makalesi |
Yazarlar |
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Destekleyen Kurum | Burdur Mehmet Akif Ersoy Üniversitesi Bilimsel Araştırma ve Projeler Koordinatörlüğü (BAP) |
Proje Numarası | 0403-DR-16 |
Teşekkür | This article was summarized from the PhD thesis by Hasbi Sait SALTIK (Burdur Mehmet Akif Ersoy University, Institute of Health Sciences). |
Yayımlanma Tarihi | 30 Aralık 2022 |
Yayınlandığı Sayı | Yıl 2023Cilt: 70 Sayı: 1 |
Bibtex | @araştırma makalesi { auvfd846475, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2023}, volume = {70}, number = {1}, pages = {49 - 56}, doi = {10.33988/auvfd.846475}, title = {Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs}, key = {cite}, author = {Saltık, Hasbi Sait and Kale, Mehmet} } |
APA | Saltık, H. S. & Kale, M. (2023). Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 70 (1) , 49-56 . DOI: 10.33988/auvfd.846475 |
MLA | Saltık, H. S. , Kale, M. "Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 (2023 ): 49-56 <http://vetjournal.ankara.edu.tr/tr/pub/issue/74067/846475> |
Chicago | Saltık, H. S. , Kale, M. "Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs". Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 (2023 ): 49-56 |
RIS | TY - JOUR T1 - Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs AU - Hasbi SaitSaltık, MehmetKale Y1 - 2023 PY - 2023 N1 - doi: 10.33988/auvfd.846475 DO - 10.33988/auvfd.846475 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 49 EP - 56 VL - 70 IS - 1 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.846475 UR - https://doi.org/10.33988/auvfd.846475 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs %A Hasbi Sait Saltık , Mehmet Kale %T Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs %D 2023 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 70 %N 1 %R doi: 10.33988/auvfd.846475 %U 10.33988/auvfd.846475 |
ISNAD | Saltık, Hasbi Sait , Kale, Mehmet . "Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs". Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 / 1 (Aralık 2022): 49-56 . https://doi.org/10.33988/auvfd.846475 |
AMA | Saltık H. S. , Kale M. Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs. Ankara Univ Vet Fak Derg. 2023; 70(1): 49-56. |
Vancouver | Saltık H. S. , Kale M. Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2023; 70(1): 49-56. |
IEEE | H. S. Saltık ve M. Kale , "Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 70, sayı. 1, ss. 49-56, Ara. 2022, doi:10.33988/auvfd.846475 |