Araştırma Makalesi
Yıl 2024,
Cilt: 71 Sayı: 3, 349 - 356, 10.07.2024
Öz
The main objective of sperm production centers is to produce as many straws as possible from the obtained ejaculates using the optimal dilution rate. To this end, this study is the first to evaluate the effect of different semen extender rates on Anatolian buffalo semen quality. Ejaculates were collected by artificial vagina from three Anatolian buffalo bulls. These ejaculates were divided into three aliquots and filled into 0.25 ml straws with soy-based extenders at concentrations of 35, 25, and 15 million sperm/straw (n=105). The straw samples of different sperm concentrations were frozen. The quality of sperm was evaluated after thawing (37 °C, 30 sec) and following the thermoresistance test (37 °C, 3 h). The post-thaw total motility and progressive motility values were similar between the groups. However, following the thermoresistance test, there was a significant decrease in total motility in the 35 million sperm/straw group, and the progressive motility was significantly higher in the 25 million sperm/straw group. There was no statistically significant difference between the groups in terms of sperm kinetic parameters, except for VSL after thawing, as well as VAP and LIN values following the thermoresistance test. The overall mean PMAI and STR values were the highest in the 25 million sperm/straw group. In conclusion, it is recommended to dilute the Anatolian buffalo semen at a concentration of 25 million/0.25 ml when freezing it with a soy-based semen extender. In addition, it is considered that soy-based extenders compensate for cryo-damage to sperm motility for a short time, and the thermoresistance test should be applied for objective evaluation in dose optimization studies.
Anahtar Kelimeler
Bubalis bubalis Computer-assisted sperm analysis flow cytometer frozen semen semen extender
Etik Beyan
This study was approved by the Animal Experiments Local Ethics Committee of the International Center for Livestock Research and Training Institute, Ankara (Decision Number: 26.12.2018-160).
Teşekkür
This study was supported by the Directorate of the International Center for Livestock Research and Training Institute, Ankara, Türkiye. The authors thank the Department of Biotechnology and Artificial Insemination, the International Center for Livestock Research and Training Institute for their support and assistance. The preliminary results of this study were presented as a poster at the 12th World Buffalo Congress in Istanbul, Türkiye held on September 18-20, 2019.
Kaynakça
- Ahmed H, Jahan S, Khan A, et al (2020): Supplementation of l-tryptophan (an aromatic amino acid) in tris citric acid extender enhances post-thaw progressive motility, plasmalemma, mitochondrial membrane potential, acrosome, and DNA integrities, and in vivo fertility rate of buffalo (Bubalus bubalis) bull spermatozoa. Cryobiology, 92, 117-123.
- Ansari MS, Rakha BA, Akhter S (2017): Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation. Reprod Domest Anim, 52, 992-997.
- Arjun V, Kumar P, Dutt R, et al (2021): Is addition or removal of seminal plasma able to compensate for the dilution effect of buffalo semen? Andrologia, 53, e14123.
- Basioura A, Tsakmakidis IA, Martinez E, et al (2020): Effect of astaxanthin in extenders on sperm quality and functional variables of frozen-thawed boar semen. Anim Reprod Sci, 218, 106478.
- Bastan I, Korkmaz F, Sahin D, et al (2021): First commercial semen cryopreservation and main spermatological features of Anatolian buffalo. Livestock Studies, 61, 060-065.
- Dejarnette JM, Nebel RL, Marshall CE, et al (2008): Effect of sex-sorted sperm dosage on conception rates in Holstein heifers and lactating cows. J Dairy Sci, 91, 1778-1785.
- Evans HC, Dinh TT, Ugur MR, et al (2020): Lipidomic markers of sperm cry tolerance in cattle. Sci Rep, 10, 1-9.
- Funk DA (2006): Major advances in globalization and consolidation of the artificial insemination industry. J Dairy Sci, 89, 1362-1368.
- Gaviraghi, A, Puglisi R, Balduzzi D, et al (2013): A minimum number of spermatozoa per dose in Mediterranean Italian buffalo (Bubalus bubalis) using sexed frozen semen and conventional artificial insemination. Theriogenology, 79, 1171-1176.
- Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, et al (2022): Seminal plasma proteins as potential biomarkers for sperm motility and velocities. Theriogenology, 177, 34-41.
- Gurupriya VS, Divyashree BC, Roy SC (2014): Cryogenic changes in proteases and antiprotease activities of buffalo (Bubalus bubalis) and cattle (Bos taurus) semen. Theriogenology, 81, 396-402.
- Juyena NS, Stelletta C (2012): Seminal plasma: an essential attribute to spermatozoa. J Androl, 33, 536-551.
- Karan P, Mohanty TK, Kumaresan A, et al (2018): Improvement in sperm functional competence through modified low‐dose packaging in French mini straws of bull semen. Andrologia, 50, e13003.
- Kulaksız R, Çebi Ç, Akçay E, et al (2010): The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Rumin Res, 88, 12-15.
- Kogan T, Grossman Dahan D, Laor R, et al (2021): Association between fatty acid composition, cryotolerance, and fertility competence of progressively motile bovine spermatozoa. Animals, 11, 2948.
- Korkmaz F, Malama E, Siuda M, et al (2017): Effects of sodium pyruvate on viability, synthesis of reactive oxygen species, lipid peroxidation, and DNA integrity of cryopreserved bovine sperm. Anim Reprod Sci, 185, 18-27.
- Lone SA, Mohanty TK, Bhakat M, et al (2020): Modification of French mini-straw plug position for cryopreservation of small doses of bull sperm. Anim Reprod Sci, 218, 106485.
- Minervini F, Guastamacchia R, Pizzi F, et al (2013): Assessment of different functional parameters of frozen–thawed buffalo spermatozoa by using cytofluorimetric determinations. Reprod Domest Anim, 48, 317-324.
- Mortimer ST, Swan MA (1999): Effect of image sampling frequency on established and smoothing-independent kinematic values of capacitating human spermatozoa. Hum Reprod, 14, 997-1004.
- Nagy Á, Polichronopoulos T, Gáspárdy A, et al (2015): Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis. Acta Vet Hung, 63, 370-381.
- Neglia G, Nicola D, Esposito L, et al (2020): Reproductive management in buffalo by artificial insemination. Theriogenology, 150,166-172.
- Ntemka A, Tsousis G, Brozos C, et al (2016): Breed differences of bull frozen‐thawed semen. Reprod Domest Anim, 51, 945-952.
- Patil S, Kumar P, Singh G, et al (2020): Semen dilution effect on sperm variables and conception rate in buffalo. Anim Reprod Sci, 214, 106304.
- Peddinti D, Nanduri B, Kaya A, et al (2008): Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. BMC Syst Biol, 2, 1-13.
- Polge C, Smith A, Parkes A, (1949): Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature, 164, 666.
- Restrepo G, Varela E, Duque JE, et al (2019): Freezing, vitrification, and freeze-drying of equine spermatozoa: Impact on mitochondrial membrane potential, lipid peroxidation, and DNA integrity. J Equine Vet Sci, 72, 8-15.
- Roy A, Srivastava RK, Pandey MD (1956): Deep freezing of buffalo semen diluted and preserved in glycine–egg yolk medium. Ind J Dairy Sci, 9, 61-62.
- Sahi̇n D, Bastan I, Ci̇l B, et al (2020): The number of false mountings affects the quality of semen in bulls. Livestock Studies, 60, 9-14.
- Singh AK, Kumar A, Honparkhe M, et al (2018): Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk, soya bean lecithin‐and liposome‐based extenders. Reprod Domest Anim, 53, 195-202.
- Soysal M, Ozkan E, Kok S, et al (2007): Genetic characterization of indigenous Anatolian water buffalo breed using microsatellite DNA markers. Ital J Anim Sci, 6, 409-412.
- Tekin K, Daşkın A (2019): Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation. Cryobiology, 89, 60-67.
- Ugur MR, Saber Abdelrahman A, Evans HC, et al (2019): Advances in cryopreservation of bull sperm. Front Vet Sci, 6, 268.
- Vera-Munoz O, Amirat-Briand L, Diaz T, et al (2009): Effect of semen dilution to low-sperm number per dose on motility and functionality of cryopreserved bovine spermatozoa using low-density lipoproteins (LDL) extender: Comparison to Triladyl® and Bioxcell®. Theriogenology, 71, 895-900.
- Vincent P, Underwood SL, Dolbec C, et al (2014): Bovine semen quality control in artificial insemination centers. 1019-1031. In: Richard M. Hopper (Ed), Bovine Reproduction.
Yıl 2024,
Cilt: 71 Sayı: 3, 349 - 356, 10.07.2024
Öz
Kaynakça
- Ahmed H, Jahan S, Khan A, et al (2020): Supplementation of l-tryptophan (an aromatic amino acid) in tris citric acid extender enhances post-thaw progressive motility, plasmalemma, mitochondrial membrane potential, acrosome, and DNA integrities, and in vivo fertility rate of buffalo (Bubalus bubalis) bull spermatozoa. Cryobiology, 92, 117-123.
- Ansari MS, Rakha BA, Akhter S (2017): Cryopreservation of Nili‐Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation. Reprod Domest Anim, 52, 992-997.
- Arjun V, Kumar P, Dutt R, et al (2021): Is addition or removal of seminal plasma able to compensate for the dilution effect of buffalo semen? Andrologia, 53, e14123.
- Basioura A, Tsakmakidis IA, Martinez E, et al (2020): Effect of astaxanthin in extenders on sperm quality and functional variables of frozen-thawed boar semen. Anim Reprod Sci, 218, 106478.
- Bastan I, Korkmaz F, Sahin D, et al (2021): First commercial semen cryopreservation and main spermatological features of Anatolian buffalo. Livestock Studies, 61, 060-065.
- Dejarnette JM, Nebel RL, Marshall CE, et al (2008): Effect of sex-sorted sperm dosage on conception rates in Holstein heifers and lactating cows. J Dairy Sci, 91, 1778-1785.
- Evans HC, Dinh TT, Ugur MR, et al (2020): Lipidomic markers of sperm cry tolerance in cattle. Sci Rep, 10, 1-9.
- Funk DA (2006): Major advances in globalization and consolidation of the artificial insemination industry. J Dairy Sci, 89, 1362-1368.
- Gaviraghi, A, Puglisi R, Balduzzi D, et al (2013): A minimum number of spermatozoa per dose in Mediterranean Italian buffalo (Bubalus bubalis) using sexed frozen semen and conventional artificial insemination. Theriogenology, 79, 1171-1176.
- Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, et al (2022): Seminal plasma proteins as potential biomarkers for sperm motility and velocities. Theriogenology, 177, 34-41.
- Gurupriya VS, Divyashree BC, Roy SC (2014): Cryogenic changes in proteases and antiprotease activities of buffalo (Bubalus bubalis) and cattle (Bos taurus) semen. Theriogenology, 81, 396-402.
- Juyena NS, Stelletta C (2012): Seminal plasma: an essential attribute to spermatozoa. J Androl, 33, 536-551.
- Karan P, Mohanty TK, Kumaresan A, et al (2018): Improvement in sperm functional competence through modified low‐dose packaging in French mini straws of bull semen. Andrologia, 50, e13003.
- Kulaksız R, Çebi Ç, Akçay E, et al (2010): The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Rumin Res, 88, 12-15.
- Kogan T, Grossman Dahan D, Laor R, et al (2021): Association between fatty acid composition, cryotolerance, and fertility competence of progressively motile bovine spermatozoa. Animals, 11, 2948.
- Korkmaz F, Malama E, Siuda M, et al (2017): Effects of sodium pyruvate on viability, synthesis of reactive oxygen species, lipid peroxidation, and DNA integrity of cryopreserved bovine sperm. Anim Reprod Sci, 185, 18-27.
- Lone SA, Mohanty TK, Bhakat M, et al (2020): Modification of French mini-straw plug position for cryopreservation of small doses of bull sperm. Anim Reprod Sci, 218, 106485.
- Minervini F, Guastamacchia R, Pizzi F, et al (2013): Assessment of different functional parameters of frozen–thawed buffalo spermatozoa by using cytofluorimetric determinations. Reprod Domest Anim, 48, 317-324.
- Mortimer ST, Swan MA (1999): Effect of image sampling frequency on established and smoothing-independent kinematic values of capacitating human spermatozoa. Hum Reprod, 14, 997-1004.
- Nagy Á, Polichronopoulos T, Gáspárdy A, et al (2015): Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis. Acta Vet Hung, 63, 370-381.
- Neglia G, Nicola D, Esposito L, et al (2020): Reproductive management in buffalo by artificial insemination. Theriogenology, 150,166-172.
- Ntemka A, Tsousis G, Brozos C, et al (2016): Breed differences of bull frozen‐thawed semen. Reprod Domest Anim, 51, 945-952.
- Patil S, Kumar P, Singh G, et al (2020): Semen dilution effect on sperm variables and conception rate in buffalo. Anim Reprod Sci, 214, 106304.
- Peddinti D, Nanduri B, Kaya A, et al (2008): Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. BMC Syst Biol, 2, 1-13.
- Polge C, Smith A, Parkes A, (1949): Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature, 164, 666.
- Restrepo G, Varela E, Duque JE, et al (2019): Freezing, vitrification, and freeze-drying of equine spermatozoa: Impact on mitochondrial membrane potential, lipid peroxidation, and DNA integrity. J Equine Vet Sci, 72, 8-15.
- Roy A, Srivastava RK, Pandey MD (1956): Deep freezing of buffalo semen diluted and preserved in glycine–egg yolk medium. Ind J Dairy Sci, 9, 61-62.
- Sahi̇n D, Bastan I, Ci̇l B, et al (2020): The number of false mountings affects the quality of semen in bulls. Livestock Studies, 60, 9-14.
- Singh AK, Kumar A, Honparkhe M, et al (2018): Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk, soya bean lecithin‐and liposome‐based extenders. Reprod Domest Anim, 53, 195-202.
- Soysal M, Ozkan E, Kok S, et al (2007): Genetic characterization of indigenous Anatolian water buffalo breed using microsatellite DNA markers. Ital J Anim Sci, 6, 409-412.
- Tekin K, Daşkın A (2019): Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation. Cryobiology, 89, 60-67.
- Ugur MR, Saber Abdelrahman A, Evans HC, et al (2019): Advances in cryopreservation of bull sperm. Front Vet Sci, 6, 268.
- Vera-Munoz O, Amirat-Briand L, Diaz T, et al (2009): Effect of semen dilution to low-sperm number per dose on motility and functionality of cryopreserved bovine spermatozoa using low-density lipoproteins (LDL) extender: Comparison to Triladyl® and Bioxcell®. Theriogenology, 71, 895-900.
- Vincent P, Underwood SL, Dolbec C, et al (2014): Bovine semen quality control in artificial insemination centers. 1019-1031. In: Richard M. Hopper (Ed), Bovine Reproduction.
Toplam 34 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Bilimleri (Diğer) |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Erken Görünüm Tarihi | 21 Eylül 2023 |
| Yayımlanma Tarihi | 10 Temmuz 2024 |
| Yayımlandığı Sayı | Yıl 2024Cilt: 71 Sayı: 3 |
