Öz
ABSTRACT
The present study was to investigate the incidence of Helicobacter pullorum in caecum and colon of 96 broiler chickens. All samples were obtained from different commercial broiler slaughtering facilities in Marmara region of Turkey. The presence of H. pullorum was assessed by conventional culture method and specific polymerase chain reaction (PCR) test. H. pullorum were not isolated from any of the samples. In contrast, H. pullorum DNA was detected by PCR in 55.21% (53/96) of the samples. Also, it was indicated that caecum was commonly infected than colon. The results showed that H. pullorum is present in Marmara region of Turkey. Further studies are needed to identify the risk factors for the presence of H. pullorum for human health.
Key Words: Helicobacter pullorum, chicken, intestine, isolation, PCR
ÖZET
MARMARA BÖLGESİNDE TAVUKLARDA HELİCOBACTER PULLORUM'UN VARLIĞININ ARAŞTIRILMASI
Bu çalışmada 96 etçi tavuğa ait sekum ve kolon örneklerinde Helicobacter pullorum'un insidensi araştırıldı. Örnekler Türkiye'nin Marmara bölgesindeki farklı ticari etçi kesimhanelerden toplandı. H. pullorum varlığı konvansiyonel kültürel metotlar ve spesifik Polimeraz zincir reaksiyonu (PCR) ile araştırıldı. Örneklerden H. pullorum izole edilmedi. Ancak H. pullorum DNA'sı PCR ile örneklerin %55,21 (53/96)'inde saptandı. Etken sekumda kolondan daha yüksek oranda belirlendi. Bu çalışmada sonuçlar Marmara bölgesinde H. pullorum varlığını ortaya koydu. İnsan sağlığı için H. pullorum'un risk faktörü olup olmadığının saptanması için konu ile ilgili araştırmaların sürdürülmesinin gerekli olduğu sonucuna varıldı.
Anahtar Kelimeler: Helicobacter pullorum, tavuk, bağırsak, izolasyon, PCR
Anahtar Kelimeler
Kaynakça
- Andersen, L.P., 2001. New Helicobacter species in humans. Digestive Diseases 19, 112-115.
- Atabay, H.I., Corry, J . E . L . , On, S.L.W., 1998. Identification of unusual Campylobacter-like isolates from poultry products as Helicobacter pullorum. Journal of Applied Microbiology 84, 1017-1024.
- Burnens, A.P., Stanley, J . , Nicolet, J , 1996. Possible association of Helicobacter pullorum with lesions of vibrionic hepatitis in poultry. In: Newell et al. (Eds), Campylobacters, Helicobacters and related organism. New York Plenum pres, 291-293.
- Ceelen, L.M., Decostere, A., Van den Bulck, K., Stephen, L.W., Margo, B., Ducatelle, R., Haesebrouck, F., 2006. Helicobacter pullorum in Chickens, Belgium. Emerging Infectious Diseases 12, 263-267.
- Fox, J.G., Lee, A., 1997. The role of Helicobacter species in nely recognized gastrointestinal tract diseases of animals. Laboratory Animal Science 47, 222-225.
- Manfreda, G., Rossi, M., Sanguinetti, V., Gavioli, R., Lozito, P., De Cesare, A., Zanoni, R.G., 2006. Prevalence of Helicobacter pullorum in broiler chickens reared in intensive and extensive farms. World's Poultry Science Journal 62, 551-552.
- Miller, K.A., Blackall, L . L . , Miflin, J.K., Templeton, J.M., Blackall, P.J., 2006. Detection of Helicobacter pullorum in meat chickens in Australia. Australian Veterinary Journal 84, 95-97.
- Neubauer, C., Hess, M., 2006. Tissue tropism of Helicobacter pullorum in specified pathogen- free chickens determined by culture and nucleic acid detection. Avian Disease 50, 620-623.
- Pilon, C., Prouzel-Mauleon, V., Menard, A., Megraud, F., 2005. Development of a real-time quantitative PCR specific to Helicobacter pullorum. international Workshop on Campylobacter, Helicobacter and Related Abstracts of Scientific Presentations, September 4-Thirteenth Organisms (CHRO). Abstracts of Scientific Presentations, September 4-8th, Gold Coast, Queensland, Australia, pp. 62.
- Rocha, M., Avenaud, P., Menard, A., Le Bail, B., Balabaud, C., Bioulac-Sage, P., de Magalhaes Queiroz, D.M., Megraud, F . , 2004. Association of Helicobacter species with hepatitis C cirrhosis with or without hepatocellular carcinoma. Gut 54, 396-401.
- Stanley, J . , Linton, D., Burnens, A.P., Dewhirst, F . E . , On, S.L.W., Porter, A., 1994. Helicobacter pullorum sp. nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology 140, 3441-3449.
- Steele, T.W., McDermott, S.N., 1984. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces, Pathology, 16, 263-265.
- Steinbrueckner, B.G., Hearter, K., Pelz, S., Weiner, J.A., Rump, W., Deissler, S., Bereswill, M., 1997. Isolation of Helicobacter pullorum from patients with enteritis. Scandinavian Journal of Infectious Disease 29, 315-318.
- Zanoni, R . G . , Rossi, M., Giacomucci, D., Sanguinetti, V., Manfreda G., 2007. Occurence and antibiotic susceptibility of Helicobacter pullorum from broiler chickens and commercial laying hens in italy. International Journal of Food Microbiology 116, 168-173.
Öz
The present study was to investigate the incidence of Helicobacter pullorum in caecum and colon of 96 broiler chickens. A l l samples were obtained from different commercial broiler slaughtering facilities in Marmara region of Turkey. The presence of H. pullorum was assessed by conventional culture method and specific polymerase chain reaction (PCR) test. H. pullorum were not isolated from any of the samples. In contrast, H. pullorum DNA was detected by PCR in 55.21% (53/96) of the samples. Also, it was indicated that caecum was commonly infected than colon. The results showed that H. pullorum is present in Marmara region of Turkey. Further studies are needed to identify the risk factors for the presence of H. pullorum for human health
Anahtar Kelimeler
Kaynakça
- Andersen, L.P., 2001. New Helicobacter species in humans. Digestive Diseases 19, 112-115.
- Atabay, H.I., Corry, J . E . L . , On, S.L.W., 1998. Identification of unusual Campylobacter-like isolates from poultry products as Helicobacter pullorum. Journal of Applied Microbiology 84, 1017-1024.
- Burnens, A.P., Stanley, J . , Nicolet, J , 1996. Possible association of Helicobacter pullorum with lesions of vibrionic hepatitis in poultry. In: Newell et al. (Eds), Campylobacters, Helicobacters and related organism. New York Plenum pres, 291-293.
- Ceelen, L.M., Decostere, A., Van den Bulck, K., Stephen, L.W., Margo, B., Ducatelle, R., Haesebrouck, F., 2006. Helicobacter pullorum in Chickens, Belgium. Emerging Infectious Diseases 12, 263-267.
- Fox, J.G., Lee, A., 1997. The role of Helicobacter species in nely recognized gastrointestinal tract diseases of animals. Laboratory Animal Science 47, 222-225.
- Manfreda, G., Rossi, M., Sanguinetti, V., Gavioli, R., Lozito, P., De Cesare, A., Zanoni, R.G., 2006. Prevalence of Helicobacter pullorum in broiler chickens reared in intensive and extensive farms. World's Poultry Science Journal 62, 551-552.
- Miller, K.A., Blackall, L . L . , Miflin, J.K., Templeton, J.M., Blackall, P.J., 2006. Detection of Helicobacter pullorum in meat chickens in Australia. Australian Veterinary Journal 84, 95-97.
- Neubauer, C., Hess, M., 2006. Tissue tropism of Helicobacter pullorum in specified pathogen- free chickens determined by culture and nucleic acid detection. Avian Disease 50, 620-623.
- Pilon, C., Prouzel-Mauleon, V., Menard, A., Megraud, F., 2005. Development of a real-time quantitative PCR specific to Helicobacter pullorum. international Workshop on Campylobacter, Helicobacter and Related Abstracts of Scientific Presentations, September 4-Thirteenth Organisms (CHRO). Abstracts of Scientific Presentations, September 4-8th, Gold Coast, Queensland, Australia, pp. 62.
- Rocha, M., Avenaud, P., Menard, A., Le Bail, B., Balabaud, C., Bioulac-Sage, P., de Magalhaes Queiroz, D.M., Megraud, F . , 2004. Association of Helicobacter species with hepatitis C cirrhosis with or without hepatocellular carcinoma. Gut 54, 396-401.
- Stanley, J . , Linton, D., Burnens, A.P., Dewhirst, F . E . , On, S.L.W., Porter, A., 1994. Helicobacter pullorum sp. nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology 140, 3441-3449.
- Steele, T.W., McDermott, S.N., 1984. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces, Pathology, 16, 263-265.
- Steinbrueckner, B.G., Hearter, K., Pelz, S., Weiner, J.A., Rump, W., Deissler, S., Bereswill, M., 1997. Isolation of Helicobacter pullorum from patients with enteritis. Scandinavian Journal of Infectious Disease 29, 315-318.
- Zanoni, R . G . , Rossi, M., Giacomucci, D., Sanguinetti, V., Manfreda G., 2007. Occurence and antibiotic susceptibility of Helicobacter pullorum from broiler chickens and commercial laying hens in italy. International Journal of Food Microbiology 116, 168-173.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Kasım 2012 |
| Yayımlandığı Sayı | Yıl 2013 Cilt: 39 Sayı: 1 |