Araştırma Makalesi
Levels of leukocyte oxidative DNA damage (8-OHdG), serum coenzyme Q10 and lipid peroxidation in the formation attacks of patients with multiple sclerosis
Öz
Multiple sclerosis (MS) is a demyelinating disease
of the nervous system. Evidence about oxidative stress plays an important role
in the pathogenesis of MS is increasing day by day. In our study, we aimed to
investigate the effect of oxidative DNA damage and oxidative stress in the
pathogenesis of MS disease. Blood samples were obtained from during an attack
(Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and
30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of
oxidized lipids were detected using fluorescence dedector with high pressure
liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and
patients with MS and then we measured 8-hydroxy- 2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and
UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and
reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum
MDA level of group 1 was significantly higher than those in group 2 and group 3
(p< 0.001). 8- OHdG/106 dG ratio
of group 1 was significantly higher than those in group 2 and group 3 (p<
0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared
with group 2 and group 3 (p<0.001). In conclusion, we observed that
oxidative DNA damage, lipid and mitochondria oxidative damage were high in
blood of patients with MS. It seems that oxidative stress acts a play role the
pathogenesis of MS patients as well as induces attacks.
Kaynakça
- Acar A, Ugur Cevik M, Evliyaoglu O, Uzar E, Tamam Y, Arikanoğlu A et al. 2012. Evaluation of serum oxidant/antioxidant balance in multiple sclerosis. Acta Neurol Belg 112: 275-80.
- Arı E, Kaya Y, Demir H, Cebi A, Alp HH, Bakan E, Odabası D, Keskin S. 2011. Oxidative DNA damage correlates with carotid artery atherosclerosis in hemodialysis patients. Hemodial Int 15: 453-459.
- Ates O, Alp HH, Kocer I, Baykal O and Salman IA. 2010. Oxidative DNA damage in patients with cataract. Acta Ophthalmol 88: 891-895.
- Besler HT, Comoglu S and Okcu Z. 2002. Serum levels of antioxidant vitamins and lipid peroxidation in multiple sclerosis. Nutr Neurosci 5: 215-20.
- Chinnery P, Majamaa K, Turnbull D, Thorburn D. 2006. Treatment for mitochondrial disorders. Cochrane DB SYST Rev 1: article number CD004426.
- Cobanoglu U, Demir H, Cebi A, Sayir F, Alp HH, Akan Z, Gur T, Bakan E. 2011. Lipid peroxidation, DNA damage and coenzyme Q10 in lung cancer patients--markers for risk assessment? Asian Pac J Cancer Prev. 12(6):1399-1403.
- Frohlich DA, McCabe MT, Arnold RS and Day ML. 2008. The role of Nrf2 in increased reactive oxygen species and DNA damage in prostate tumorigenesis. Oncogene 27: 4353-4362
- Gilgun-Sherki Y, Melamed E, Offen D. 2004. The role of oxidative stress in the pathogenesis of multiple sclerosis: The need for effective antioxidant therapy. J Neurol 251: 261-268.
- Gonsette RE. 2008. Neurodegeneration in multiple sclerosis: The role of oxidative stress and excitotoxicity. J Neurol Sci 274:48-53.
- Grotto D, Maria LS, Valentini J et al. 2009. Importance of the Lipid Peroxidation Biomarkers and Methodological Aspects for Malondialdehyde Quantification. Quim Nova 32: 169-174.
- Huyut Z, Şekeroğlu MR, Balaharoğlu R, Karakoyun T, Çokluk E. 2016a. The relationship of oxidation sensitivity of red blood cells and carbonic anhydrase activity in stored human blood; Effect of certain phenolic compounds. BioMed Res Internat Article ID 3057384
- Huyut Z, Şekeroğlu MR, Balahoroğlu R, Alp HH, Çokluk E. 2016b. In stored human blood, the inhibitör effect of tannic acid and caffeic acid on lipid peroxidation and oxidative DNA damage. East J Med, 21(2): 88-93.
- Irshad M, Chaudhuri PS. 2002. Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol 40:1233-9.
- Kaur H, Halliwell B. 1996. Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection. Biochem J 318:21-23.
- Kaya Y, Çebi A, Söylemez N, Demir H, Alp HH, Bakan E. 2012. Correlations between oxidative DNA damage, oxidative stress and coenzyme Q10 in patients with coronary artery disease. Int J Med Sci. 9(8):621-626.
- Khoschsorur GA, Winklhofer-Roob BM, Rabl H, Auer T, Peng Z, Schaur RJ. 2000. Evaluation of a sensitive HPLC method for the determination of malondialdehyde, and application of the method to different biological materials. Chromatographia 52:181-184.
- Koch M, Ramsaransing GS, Arutjunyan AV, Stepanov M, Teelken A, Heersema DJ, De Keyser J. 2006. Oxidative stress in serum and peripheral blood leukocytes in patients with different disease courses of multiple sclerosis. J Neurol 253:483-487.
- Korpela H, Kinnunen E, Juntunen J, Kumpulainen J, Koskenvuo M. 1989. Serum selenium concentration, glutathione peroxidase activity and lipid peroxides in a co-twin control study on multiple sclerosis. J Neurol Sci. 91(1-2):79-84.
- Lassmann H, van Horssen J, Mahad D. 2012. Progressive multiple sclerosis: pathology and pathogenesis. Nat Rev Neurol. 8(11):647-656.
- Mahad DH, Trapp Bruce D, Lassmann H. 2015. Pathological mechanisms in progressive multiple sclerosis. Lancet Neurol 14:183-193.
- Manczak M, Mao P, Calkins MJ et al. 2010. Mitochondria-targeted antioxidants protect against amyloid-beta toxicity in Alzheimer's disease neurons. J Alzheimers Dis 20:609-631.
- Mao P, Manczak M, Shirendeb UP and Reddy PH. 2013. MitoQ, a mitochondria-targeted antioxidant, delays disease progression and alleviates pathogenesis in an experimental autoimmune encephalomyelitis mouse model of multiple sclerosis. BBA Mol Basis Dis 1832: 2322-31.
- McDonald WI, Compston A, Edan G, Goodkin D, Hartung HP, Lublin FD et al. 2001. Recommended diagnostic criteria for multiple sclerosis: Guidelines from the International Panel on the Diagnosis of Multiple Sclerosis. Ann Neurol 50:121-127.
- Miller A. 1999. Continuum: Multiple Sclerosis (Part A). 5:7.
- Ostman B, Sjodin A, Michaelsson K, Byberg L. 2012. Coenzyme Q10 supplementation and exercise-induced oxidative stress in humans. Nutrition 28: 403-417.
- Smith RA and Murphy MP. 2010. Animal and human studies with the mitochondria-targeted antioxidant MitoQ. Ann NY Acad Sci 1201:96-103.
- Tarng DG, Huang TP, Wei YH, Liu TY, Chen HW, Chen TW, Yang WC. 2000. 8-hydroxy-2 '-deoxyguanosine of leukocyte DNA as a marker of oxidative stress in chronic hemodialysis patients. Am J Kidney Dis 36:934-944.
- Tasset I, Agüera E, Sánchez-López F, Feijóo M, Giraldo AI, Cruz AH, Gascón F, Túnez I. 2012. Peripheral oxidative stress in relapsing-remitting multiple sclerosis. Clin Biochem. 45(6):440-444.
Yıl 2017,
Cilt: 9 Sayı: 1, 601 - 607, 12.08.2017
Öz
Kaynakça
- Acar A, Ugur Cevik M, Evliyaoglu O, Uzar E, Tamam Y, Arikanoğlu A et al. 2012. Evaluation of serum oxidant/antioxidant balance in multiple sclerosis. Acta Neurol Belg 112: 275-80.
- Arı E, Kaya Y, Demir H, Cebi A, Alp HH, Bakan E, Odabası D, Keskin S. 2011. Oxidative DNA damage correlates with carotid artery atherosclerosis in hemodialysis patients. Hemodial Int 15: 453-459.
- Ates O, Alp HH, Kocer I, Baykal O and Salman IA. 2010. Oxidative DNA damage in patients with cataract. Acta Ophthalmol 88: 891-895.
- Besler HT, Comoglu S and Okcu Z. 2002. Serum levels of antioxidant vitamins and lipid peroxidation in multiple sclerosis. Nutr Neurosci 5: 215-20.
- Chinnery P, Majamaa K, Turnbull D, Thorburn D. 2006. Treatment for mitochondrial disorders. Cochrane DB SYST Rev 1: article number CD004426.
- Cobanoglu U, Demir H, Cebi A, Sayir F, Alp HH, Akan Z, Gur T, Bakan E. 2011. Lipid peroxidation, DNA damage and coenzyme Q10 in lung cancer patients--markers for risk assessment? Asian Pac J Cancer Prev. 12(6):1399-1403.
- Frohlich DA, McCabe MT, Arnold RS and Day ML. 2008. The role of Nrf2 in increased reactive oxygen species and DNA damage in prostate tumorigenesis. Oncogene 27: 4353-4362
- Gilgun-Sherki Y, Melamed E, Offen D. 2004. The role of oxidative stress in the pathogenesis of multiple sclerosis: The need for effective antioxidant therapy. J Neurol 251: 261-268.
- Gonsette RE. 2008. Neurodegeneration in multiple sclerosis: The role of oxidative stress and excitotoxicity. J Neurol Sci 274:48-53.
- Grotto D, Maria LS, Valentini J et al. 2009. Importance of the Lipid Peroxidation Biomarkers and Methodological Aspects for Malondialdehyde Quantification. Quim Nova 32: 169-174.
- Huyut Z, Şekeroğlu MR, Balaharoğlu R, Karakoyun T, Çokluk E. 2016a. The relationship of oxidation sensitivity of red blood cells and carbonic anhydrase activity in stored human blood; Effect of certain phenolic compounds. BioMed Res Internat Article ID 3057384
- Huyut Z, Şekeroğlu MR, Balahoroğlu R, Alp HH, Çokluk E. 2016b. In stored human blood, the inhibitör effect of tannic acid and caffeic acid on lipid peroxidation and oxidative DNA damage. East J Med, 21(2): 88-93.
- Irshad M, Chaudhuri PS. 2002. Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol 40:1233-9.
- Kaur H, Halliwell B. 1996. Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection. Biochem J 318:21-23.
- Kaya Y, Çebi A, Söylemez N, Demir H, Alp HH, Bakan E. 2012. Correlations between oxidative DNA damage, oxidative stress and coenzyme Q10 in patients with coronary artery disease. Int J Med Sci. 9(8):621-626.
- Khoschsorur GA, Winklhofer-Roob BM, Rabl H, Auer T, Peng Z, Schaur RJ. 2000. Evaluation of a sensitive HPLC method for the determination of malondialdehyde, and application of the method to different biological materials. Chromatographia 52:181-184.
- Koch M, Ramsaransing GS, Arutjunyan AV, Stepanov M, Teelken A, Heersema DJ, De Keyser J. 2006. Oxidative stress in serum and peripheral blood leukocytes in patients with different disease courses of multiple sclerosis. J Neurol 253:483-487.
- Korpela H, Kinnunen E, Juntunen J, Kumpulainen J, Koskenvuo M. 1989. Serum selenium concentration, glutathione peroxidase activity and lipid peroxides in a co-twin control study on multiple sclerosis. J Neurol Sci. 91(1-2):79-84.
- Lassmann H, van Horssen J, Mahad D. 2012. Progressive multiple sclerosis: pathology and pathogenesis. Nat Rev Neurol. 8(11):647-656.
- Mahad DH, Trapp Bruce D, Lassmann H. 2015. Pathological mechanisms in progressive multiple sclerosis. Lancet Neurol 14:183-193.
- Manczak M, Mao P, Calkins MJ et al. 2010. Mitochondria-targeted antioxidants protect against amyloid-beta toxicity in Alzheimer's disease neurons. J Alzheimers Dis 20:609-631.
- Mao P, Manczak M, Shirendeb UP and Reddy PH. 2013. MitoQ, a mitochondria-targeted antioxidant, delays disease progression and alleviates pathogenesis in an experimental autoimmune encephalomyelitis mouse model of multiple sclerosis. BBA Mol Basis Dis 1832: 2322-31.
- McDonald WI, Compston A, Edan G, Goodkin D, Hartung HP, Lublin FD et al. 2001. Recommended diagnostic criteria for multiple sclerosis: Guidelines from the International Panel on the Diagnosis of Multiple Sclerosis. Ann Neurol 50:121-127.
- Miller A. 1999. Continuum: Multiple Sclerosis (Part A). 5:7.
- Ostman B, Sjodin A, Michaelsson K, Byberg L. 2012. Coenzyme Q10 supplementation and exercise-induced oxidative stress in humans. Nutrition 28: 403-417.
- Smith RA and Murphy MP. 2010. Animal and human studies with the mitochondria-targeted antioxidant MitoQ. Ann NY Acad Sci 1201:96-103.
- Tarng DG, Huang TP, Wei YH, Liu TY, Chen HW, Chen TW, Yang WC. 2000. 8-hydroxy-2 '-deoxyguanosine of leukocyte DNA as a marker of oxidative stress in chronic hemodialysis patients. Am J Kidney Dis 36:934-944.
- Tasset I, Agüera E, Sánchez-López F, Feijóo M, Giraldo AI, Cruz AH, Gascón F, Túnez I. 2012. Peripheral oxidative stress in relapsing-remitting multiple sclerosis. Clin Biochem. 45(6):440-444.
Toplam 28 adet kaynakça vardır.
Ayrıntılar
| Bölüm | Original Articles |
|---|---|
| Yazarlar | |
| Yayımlanma Tarihi | 12 Ağustos 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 9 Sayı: 1 |
