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Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations

Yıl 2020, Cilt: 33 Sayı: 1, 145 - 148, 01.04.2020
https://doi.org/10.29136/mediterranean.633476

Öz



Nerve Growth Factor Gene (NGF)
has important functions in the maintenance of viability and proliferation of
ovarian cells. Recent studies on caprine
NGF gene revealed two SNPs
(determined by
SacII
/C291A and MboII/A705G
polymorphisms
) related with body length and litter
size in goats
. This study aimed to
determine
SacII and MboII polymorphisms of NGF in native
Turkish goat populations including Turkish Hair (HAI), Kabakulak (KBK), Honamlı
(HNM) and Norduz (NRD) by using PCR-RFLP. Amplificated PCR fragments of 808
were digested with
SacII,
while amplificated PCR fragments of 319 bp were digested with
MboII restriction enzyme in order to
detect different genotypes of
NGF gene. The “A” allele frequency ranged
from 0.500 (HAI, HNM and KBK) to 0.950 (NRD), while the “C” allele frequency
ranged from 0.025 (NRD) to 0.500 (HAI, HNM and KBK) in
NGF/SacII
polymorphism.
The “A” allele frequency ranged from 0.213 (HAI) to 1.000
(NRD), while the “G” allele frequency ranged from 0.000 (NRD) to 0.787 (HAI) in
NGF/MboII
polymorphism. Deviation from HW equilibrium was significant in
HNM goat
population (
P < 0.05). In this
study, polymorphisms of caprine
NGF gene in native Turkish goat
populations were revealed for the first
time. The
results obtained from this study showed that
NGF/SacII polymorphisms could be used for
body length in NRD population while
NGF/MboII polymorphisms could be used for litter size in HAI, HNM and
KBK populations in MAS studies.




Kaynakça

  • Albers KM, Wright DF, Dawis BM (1994) Overexpression of Nerve Growth Factor in epidermis of transgenic mice causes hypertrophy of the peripheral nervous system. The Journal of Neuroscience 14: 1422-1432.
  • An X, Bai L, Hou J, Zhao H, Peng J, Song Y, Wang J, Cao B (2013) Molecular cloning, tissue expression and SNP analysis in the goat nerve growth factor gene. Molecular Biology Reports 40: 857-863.
  • Cao GL, Chu MX, Fang L, Di R, Feng T, Li N (2010) Analysis on DNA sequence of KiSS-1 gene and its association with litter size in goats. Molecular Biology Reports 37: 3921-3929.
  • Cao GL, Chu MX, Fang L, Feng T, Di R, Li N (2011) Analysis on DNA sequence of GPR54 gene and its association with litter size in goats. Molecular Biology Reports 38: 3839-3848.
  • Chaves RN, Alves AMCV, Lima LF, Matos HMT, Rodrigues APR., Figueiredo JR (2013) Role of nerve growth factor (NGF) and its receptors in folliculogenesis. Zygote 21: 187-197.
  • Cui Y, Yan H, Wang K, Xu H, Zhang X, Zhu H, Liu J, Qu L, Lan X, Pan C (2018) Insertion/deletion within the KDM6A gene is significantly associated with litter size in goat. Frontiers in Genetics 9: 91.
  • Deng C, Ma R, Yue X, Lan X, Chen H, Lei C (2010) Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats. Genetics and Molecular Biology 33: 266-270.
  • Dissen GA, Romero C, Hirshfield AN, Ojeda SR (2001) Nerve growth factor is required for early follicular development in the mammalian ovary. Endocrinology 142: 2078-2086.
  • Miller S, Dykes D, Plesky HA (1988) Simple salting out procedure for extracting DNA from human cells. Nucleic Acids Research 16: 1215.
  • Naicy T, Venkatachalapathy T, Aravindakshan TV, Bosewell A, Silpa MV (2018) Association of a SacII polymorphism in the Nerve Growth Factor (NGF) gene exon 3 with growth traits in Indian goats. Small Ruminant Research 158: 19-21.
  • Ren L, Medan MS, Weng Q, Jin W, Li C, Watanabe G, Taya K (2005) Immunolocalization of Nerve Growth Factor (NGF) and its receptors (TrkA and p75LNGFR) in the reproductive organs of Shiba goats. Journal of Reproduction and Development 51: 399-404.
  • Yan H, Zhang F, Wang K, Liu J, Zhu H, Pan C, Qu L (2018) A novel 12 bp deletion within goat LHX4 gene significantly affected litter size. Archives Animal Breeding 61: 1-8.
  • Yeh FC, Yang RC, Boyle TBJ., Ye ZH, Mao JX (1997) POPGENE, The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
  • Yılmaz O, Kor A, Ertugrul M, Wilson T (2012) The domestic livestock resources of Turkey: goat breeds and types and their conservation status. Animal Genetic Resources 51: 105-116.
  • Wang K, Yan H, Xu H, Yang Q, Zhang S, Pan C, Chen H, Zhu H, Liu J, Qu L, Lan X (2018) A novel indel within goat casein alpha S1 gene is significantly associated with litter size. Gene 671: 161-169.
  • Wang X, Yang Q, Wang K, Yan H, Pan C, Chen H, Liu J, Zhu H, Qu L, Lan X (2019) Two strongly linked single nucleotide polymorphisms (Q320P and V397I) in GDF9 geneare associated with litter size in cashmere goats. Theriogenology 125: 115-121.
  • Wu X, Jia W, Zhang J, Li X, Pan C, Lei C, Chen H, Dang R, Lan X (2014) Determination of the novel genetic variants of goat STAT5A gene and their effects on body measurement traits in two Chinese native breeds. Small Ruminant Research 121: 232-243.
  • Zhang ZJ, Ling YH, Wang LJ, Hang YF, Guo XF, Zhang YH, Ding JP, Zhang XR (2013) Polymorphisms of the miyostatin gene (MSTN) and its relationship with growth traits in goat breeds. Genetics and Molecular Research 12: 965-971.
  • Zhang X, Wu X, Jia W, Pan C, Li X, Lei C, Chen H, Lan X (2015) Novel nucleotide variations, haplotypes structure and associations with growth related traits of goat AT Motif-Binding Factor (ATBF1) gene. Asian-Australasian Journal of Animal Science 28: 1349-1406.

Türkiye’de yetiştirilen dört keçi populasyonunda Sinir Büyüme Faktörü (NGF) geninde SacII ve MboII polimorfizmlerinin belirlenmesi

Yıl 2020, Cilt: 33 Sayı: 1, 145 - 148, 01.04.2020
https://doi.org/10.29136/mediterranean.633476

Öz

Sinir
Büyüme Faktörü (
NGF) geni yumurtalık hücrelerinin yaşamını devam
ettirmesi ve çoğalmasında önemli fonksiyonlara sahiptir. Keçi
NGF geni üzerinde
yapılan son çalışmalar, iki SNP’in (
SacII/C291A ve MboII/A705G
polimorfizmi) vücut uzunluğu ve bir batında doğan yavru sayısı ile ilişkili
olduğunu ortaya çıkarmıştır. Bu çalışmada, Türkiye’de yetiştirilen Kıl (HAI),
Kabakulak (KBK), Honamlı (HNM) ve Norduz (NRD) keçi populasyonlarının
NGF
geninde
SacII ve MboII polimorfizminin PCR-RFLP yöntemiyle
belirlenmesi amaçlanmıştır.
NGF geninde farklı genotiplerin belirlenmesi
için çoğaltılan 808 bç uzunluğundaki PCR ürünleri
SacII, 319 bç
uzunluğundaki PCR ürünleri ise
MboII restriksiyon enzimi ile
kesilmiştir.
NGF/SacII polimorfizminde A allel frekansı 0.500
(Kıl, Honamlı ve Kabakulak) ile 0.950 (Norduz) aralığında değişirken, C allel
frekansı 0.025 (Norduz) ile 0.500 (Kıl, Honamlı ve Kabakulak) aralığında
değişmiştir.
NGF/MboII polimorfizminde A allel frekansı 0.213
(Kıl) ile 1.000 (Norduz) aralığında değişirken, G allel frekansı 0.000 (Norduz)
ile 0.787 (Kıl) aralığında değişmiştir. Honamlı populasyonunda HW dengesinden
sapma önemli bulunmuştur (
P < 0.05).
Bu çalışmada, Türkiye’nin yerli keçi populasyonlarında keçi
NGF gen
polimorfizmi ilk defa ortaya çıkarılmıştır.
Bu
çalışmandan elde edilen sonuçlar,
NGF/SacII polimorfizminin Norduz popuslayonunda vücut uzunluğu, için, NGF/MboII polimorfizminin ise Kıl, Honamlı
ve Kabakulak populasyonlarında bir batında doğan yavru sayısı için markör
destekli seleksiyon (MAS) çalışmalarında kullanılabileceğini göstermiştir.

Kaynakça

  • Albers KM, Wright DF, Dawis BM (1994) Overexpression of Nerve Growth Factor in epidermis of transgenic mice causes hypertrophy of the peripheral nervous system. The Journal of Neuroscience 14: 1422-1432.
  • An X, Bai L, Hou J, Zhao H, Peng J, Song Y, Wang J, Cao B (2013) Molecular cloning, tissue expression and SNP analysis in the goat nerve growth factor gene. Molecular Biology Reports 40: 857-863.
  • Cao GL, Chu MX, Fang L, Di R, Feng T, Li N (2010) Analysis on DNA sequence of KiSS-1 gene and its association with litter size in goats. Molecular Biology Reports 37: 3921-3929.
  • Cao GL, Chu MX, Fang L, Feng T, Di R, Li N (2011) Analysis on DNA sequence of GPR54 gene and its association with litter size in goats. Molecular Biology Reports 38: 3839-3848.
  • Chaves RN, Alves AMCV, Lima LF, Matos HMT, Rodrigues APR., Figueiredo JR (2013) Role of nerve growth factor (NGF) and its receptors in folliculogenesis. Zygote 21: 187-197.
  • Cui Y, Yan H, Wang K, Xu H, Zhang X, Zhu H, Liu J, Qu L, Lan X, Pan C (2018) Insertion/deletion within the KDM6A gene is significantly associated with litter size in goat. Frontiers in Genetics 9: 91.
  • Deng C, Ma R, Yue X, Lan X, Chen H, Lei C (2010) Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats. Genetics and Molecular Biology 33: 266-270.
  • Dissen GA, Romero C, Hirshfield AN, Ojeda SR (2001) Nerve growth factor is required for early follicular development in the mammalian ovary. Endocrinology 142: 2078-2086.
  • Miller S, Dykes D, Plesky HA (1988) Simple salting out procedure for extracting DNA from human cells. Nucleic Acids Research 16: 1215.
  • Naicy T, Venkatachalapathy T, Aravindakshan TV, Bosewell A, Silpa MV (2018) Association of a SacII polymorphism in the Nerve Growth Factor (NGF) gene exon 3 with growth traits in Indian goats. Small Ruminant Research 158: 19-21.
  • Ren L, Medan MS, Weng Q, Jin W, Li C, Watanabe G, Taya K (2005) Immunolocalization of Nerve Growth Factor (NGF) and its receptors (TrkA and p75LNGFR) in the reproductive organs of Shiba goats. Journal of Reproduction and Development 51: 399-404.
  • Yan H, Zhang F, Wang K, Liu J, Zhu H, Pan C, Qu L (2018) A novel 12 bp deletion within goat LHX4 gene significantly affected litter size. Archives Animal Breeding 61: 1-8.
  • Yeh FC, Yang RC, Boyle TBJ., Ye ZH, Mao JX (1997) POPGENE, The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
  • Yılmaz O, Kor A, Ertugrul M, Wilson T (2012) The domestic livestock resources of Turkey: goat breeds and types and their conservation status. Animal Genetic Resources 51: 105-116.
  • Wang K, Yan H, Xu H, Yang Q, Zhang S, Pan C, Chen H, Zhu H, Liu J, Qu L, Lan X (2018) A novel indel within goat casein alpha S1 gene is significantly associated with litter size. Gene 671: 161-169.
  • Wang X, Yang Q, Wang K, Yan H, Pan C, Chen H, Liu J, Zhu H, Qu L, Lan X (2019) Two strongly linked single nucleotide polymorphisms (Q320P and V397I) in GDF9 geneare associated with litter size in cashmere goats. Theriogenology 125: 115-121.
  • Wu X, Jia W, Zhang J, Li X, Pan C, Lei C, Chen H, Dang R, Lan X (2014) Determination of the novel genetic variants of goat STAT5A gene and their effects on body measurement traits in two Chinese native breeds. Small Ruminant Research 121: 232-243.
  • Zhang ZJ, Ling YH, Wang LJ, Hang YF, Guo XF, Zhang YH, Ding JP, Zhang XR (2013) Polymorphisms of the miyostatin gene (MSTN) and its relationship with growth traits in goat breeds. Genetics and Molecular Research 12: 965-971.
  • Zhang X, Wu X, Jia W, Pan C, Li X, Lei C, Chen H, Lan X (2015) Novel nucleotide variations, haplotypes structure and associations with growth related traits of goat AT Motif-Binding Factor (ATBF1) gene. Asian-Australasian Journal of Animal Science 28: 1349-1406.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği
Bölüm Makaleler
Yazarlar

Eymen Demir 0000-0003-4507-7426

Bahar Argun Karslı 0000-0002-1762-9847

Taki Karslı 0000-0002-2413-1713

Murat Soner Balcıoğlu 0000-0002-9424-8319

Yayımlanma Tarihi 1 Nisan 2020
Gönderilme Tarihi 15 Ekim 2019
Yayımlandığı Sayı Yıl 2020 Cilt: 33 Sayı: 1

Kaynak Göster

APA Demir, E., Argun Karslı, B., Karslı, T., Balcıoğlu, M. S. (2020). Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations. Mediterranean Agricultural Sciences, 33(1), 145-148. https://doi.org/10.29136/mediterranean.633476
AMA Demir E, Argun Karslı B, Karslı T, Balcıoğlu MS. Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations. Mediterranean Agricultural Sciences. Nisan 2020;33(1):145-148. doi:10.29136/mediterranean.633476
Chicago Demir, Eymen, Bahar Argun Karslı, Taki Karslı, ve Murat Soner Balcıoğlu. “Determination of SacII and MboII Polymorphisms in the Nerve Growth Factor (NGF) Gene in Four Native Turkish Goat Populations”. Mediterranean Agricultural Sciences 33, sy. 1 (Nisan 2020): 145-48. https://doi.org/10.29136/mediterranean.633476.
EndNote Demir E, Argun Karslı B, Karslı T, Balcıoğlu MS (01 Nisan 2020) Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations. Mediterranean Agricultural Sciences 33 1 145–148.
IEEE E. Demir, B. Argun Karslı, T. Karslı, ve M. S. Balcıoğlu, “Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations”, Mediterranean Agricultural Sciences, c. 33, sy. 1, ss. 145–148, 2020, doi: 10.29136/mediterranean.633476.
ISNAD Demir, Eymen vd. “Determination of SacII and MboII Polymorphisms in the Nerve Growth Factor (NGF) Gene in Four Native Turkish Goat Populations”. Mediterranean Agricultural Sciences 33/1 (Nisan 2020), 145-148. https://doi.org/10.29136/mediterranean.633476.
JAMA Demir E, Argun Karslı B, Karslı T, Balcıoğlu MS. Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations. Mediterranean Agricultural Sciences. 2020;33:145–148.
MLA Demir, Eymen vd. “Determination of SacII and MboII Polymorphisms in the Nerve Growth Factor (NGF) Gene in Four Native Turkish Goat Populations”. Mediterranean Agricultural Sciences, c. 33, sy. 1, 2020, ss. 145-8, doi:10.29136/mediterranean.633476.
Vancouver Demir E, Argun Karslı B, Karslı T, Balcıoğlu MS. Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations. Mediterranean Agricultural Sciences. 2020;33(1):145-8.

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