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High Salt-Induced Hyperosmolality Reduces in Vitro Survival and Proliferation of Pre-B Cells

Yıl 2023, Cilt: 5 Sayı: Supplement (1) - Innovations in Medicine and Healthcare in the 100th Year of the Republic, 150 - 4, 19.10.2023

Mehmet Yabaş

https://doi.org/10.37990/medr.1352143

Öz

Aim: B cells of the adaptive immunity are critical for protection against the vast majority of pathogens through the production of specific antibodies. A number of signaling pathways and transcription factors control B cell development. Environmental factors, including diet, are also important in determining how B cell develop and function. Here, the effects of hyperosmolality induced by elevated salt on the survival, IL-7-induced proliferation and differentiation of pre-B cells were tested in vitro.
Material and Methods: The wk3 pre-B cell line generated from SLP65–/– mice was used. Hyperosmolality in the cell culture medium was created by increasing the salt concentration with the addition of 40 mM NaCl. Wk3 pre-B cells were cultured in standard (normal NaCl) and high salt (+40 mM NaCl) medium, followed by flow cytometric analysis.
Results: It was found that hyperosmolality caused by high salt reduced survival and induced apoptosis in wk3 pre-B cells. In addition, hyperosmolality inhibited IL-7-induced proliferation of pre-B cells. Conversely, pre-B cells treated with high salt were able to differentiate normally into IgM+ immature B cells when IL-7 was removed.
Conclusion: These findings suggest that the hyperosmolar microenvironment induced by high salt may play a key role in B cell development in the bone marrow.

Anahtar Kelimeler

Hyperosmolality pre-B cells salt B cells

Etik Beyan

Not applicable

Teşekkür

The author thanks Dr. Sebastian Herzog, the Medical University of Innsbruck, Austria for providing wk3 cells, the Department of Genetics and Bioengineering at Trakya University, Turkey for the opportunity to conduct the experiments in their research facilities, and the Technology Research Development Application and Research Center of Trakya University, Turkey for the use of the flow cytometry.

Kaynakça

  • Nutt SL, Kee BL. The transcriptional regulation of B cell lineage commitment. Immunity. 2007;26:715-25. Erratum in: Immunity. 2007;27:361.
  • Hardy RR, Hayakawa K. B cell development pathways. Annu Rev Immunol. 2001;19:595-621.
  • Herzog S, Reth M, Jumaa H. Regulation of B-cell proliferation and differentiation by pre-B-cell receptor signalling. Nat Rev Immunol. 2009;9:195-205.
  • Clark MR, Mandal M, Ochiai K, Singh H. Orchestrating B cell lymphopoiesis through interplay of IL-7 receptor and pre-B cell receptor signalling. Nat Rev Immunol. 2014;14:69-80.
  • Hendriks RW, Middendorp S. The pre-BCR checkpoint as a cell-autonomous proliferation switch. Trends Immunol. 2004;25:249-56.
  • Reth M, Nielsen P. Signaling circuits in early B-cell development. Adv Immunol. 2014;122:129-75.
  • Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment. J Exp Med. 2000;191:23-32.
  • Samitas K, Lotvall J, Bossios A. B cells: from early development to regulating allergic diseases. Arch Immunol Ther Exp (Warsz). 2010;58:209-25.
  • Calder PC. Nutrition and immunity: lessons for COVID-19. Nutr Diabetes. 2021;11:19.
  • Kotchen TA, Cowley AW Jr, Frohlich ED. Salt in health and disease--a delicate balance. N Engl J Med. 2013;368:1229-37.
  • Yabas M, Bostanci A, Aral S. ATP11C promotes the differentiation of pre-B cells into immature B cells but does not affect their IL-7-dependent proliferation. Immunol Res. 2023;71:609-16.
  • Flemming A, Brummer T, Reth M, Jumaa H. The adaptor protein SLP-65 acts as a tumor suppressor that limits pre-B cell expansion. Nat Immunol. 2003;4:38-43.
  • Lindner SE, Lohmuller M, Kotkamp B, et al. The miR-15 family reinforces the transition from proliferation to differentiation in pre-B cells. EMBO Rep. 2017;18:1604-17.
  • Binger KJ, Gebhardt M, Heinig M, et al. High salt reduces the activation of IL-4- and IL-13-stimulated macrophages. J Clin Invest. 2015;125:4223-38.
  • Kleinewietfeld M, Manzel A, Titze J, et al. Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. Nature. 2013;496:518-22.
  • Wilck N, Matus MG, Kearney SM, et al. Salt-responsive gut commensal modulates TH17 axis and disease. Nature. 2017;551:585-9.
  • Hernandez AL, Kitz A, Wu C, et al. Sodium chloride inhibits the suppressive function of FOXP3+ regulatory T cells. J Clin Invest. 2015;125:4212-22.
  • Jobin K, Stumpf NE, Schwab S, et al. A high-salt diet compromises antibacterial neutrophil responses through hormonal perturbation. Sci Transl Med. 2020;12:eaay3850.
  • Machnik A, Neuhofer W, Jantsch J, et al. Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism. Nat Med. 2009;15:545-52.
  • Cvetkovic L, Perisic S, Titze J, et al. The impact of hyperosmolality on activation and differentiation of B lymphoid cells. Front Immunol. 2019;10:828.
  • Wiig H, Schroder A, Neuhofer W, et al. Immune cells control skin lymphatic electrolyte homeostasis and blood pressure. J Clin Invest. 2013;123:2803-15.
  • Quah BJ, Parish CR. New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes. J Immunol Methods. 2012;379:1-14.
  • He W, Xu J, Mu R, et al. High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation. Nat Commun. 2020;11:1732.
  • Tan M, He FJ, Wang C, MacGregor GA. Twenty-four-hour urinary sodium and potassium excretion in China: a systematic review and meta-analysis. J Am Heart Assoc. 2019;8:e012923.
  • Jobin K, Muller DN, Jantsch J, Kurts C. Sodium and its manifold impact on our immune system. Trends Immunol. 2021;42:469-79.
  • Toussirot E, Bereau M, Vauchy C, Saas P. Could sodium chloride be an environmental trigger for immune-mediated diseases? An overview of the experimental and clinical evidence. Front Physiol. 2018;9:440.
  • Wilck N, Balogh A, Marko L, et al. The role of sodium in modulating immune cell function. Nat Rev Nephrol. 2019;15:546-58.
  • Jantsch J, Schatz V, Friedrich D, et al. Cutaneous Na+ storage strengthens the antimicrobial barrier function of the skin and boosts macrophage-driven host defense. Cell Metab. 2015;21:493-501.
  • Zhang WC, Du LJ, Zheng XJ, et al. Elevated sodium chloride drives type I interferon signaling in macrophages and increases antiviral resistance. J Biol Chem. 2018;293:1030-9.
  • Willebrand R, Hamad I, Van Zeebroeck L, et al. High salt inhibits tumor growth by enhancing anti-tumor immunity. Front Immunol. 2019;10:1141.
  • Na SY, Janakiraman M, Leliavski A, Krishnamoorthy G. High-salt diet suppresses autoimmune demyelination by regulating the blood-brain barrier permeability. Proc Natl Acad Sci U S A. 2021;118:e2025944118.
  • Wu C, Yosef N, Thalhamer T, et al. Induction of pathogenic TH17 cells by inducible salt-sensing kinase SGK1. Nature. 2013;496:513-7.
  • Hucke S, Eschborn M, Liebmann M, et al. Sodium chloride promotes pro-inflammatory macrophage polarization thereby aggravating CNS autoimmunity. J Autoimmun. 2016;67:90-101.
  • Li X, Alu A, Wei Y, et al. The modulatory effect of high salt on immune cells and related diseases. Cell Prolif. 2022;55:e13250.
  • Kino T, Takatori H, Manoli I, et al. Brx mediates the response of lymphocytes to osmotic stress through the activation of NFAT5. Sci Signal. 2009;2:ra5.
  • Milne CD, Paige CJ. IL-7: a key regulator of B lymphopoiesis. Semin Immunol. 2006;18:20-30.
  • Corfe SA, Paige CJ. The many roles of IL-7 in B cell development; mediator of survival, proliferation and differentiation. Semin Immunol. 2012;24:198-208.

Yıl 2023, Cilt: 5 Sayı: Supplement (1) - Innovations in Medicine and Healthcare in the 100th Year of the Republic, 150 - 4, 19.10.2023

Mehmet Yabaş

https://doi.org/10.37990/medr.1352143

Öz

Kaynakça

  • Nutt SL, Kee BL. The transcriptional regulation of B cell lineage commitment. Immunity. 2007;26:715-25. Erratum in: Immunity. 2007;27:361.
  • Hardy RR, Hayakawa K. B cell development pathways. Annu Rev Immunol. 2001;19:595-621.
  • Herzog S, Reth M, Jumaa H. Regulation of B-cell proliferation and differentiation by pre-B-cell receptor signalling. Nat Rev Immunol. 2009;9:195-205.
  • Clark MR, Mandal M, Ochiai K, Singh H. Orchestrating B cell lymphopoiesis through interplay of IL-7 receptor and pre-B cell receptor signalling. Nat Rev Immunol. 2014;14:69-80.
  • Hendriks RW, Middendorp S. The pre-BCR checkpoint as a cell-autonomous proliferation switch. Trends Immunol. 2004;25:249-56.
  • Reth M, Nielsen P. Signaling circuits in early B-cell development. Adv Immunol. 2014;122:129-75.
  • Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment. J Exp Med. 2000;191:23-32.
  • Samitas K, Lotvall J, Bossios A. B cells: from early development to regulating allergic diseases. Arch Immunol Ther Exp (Warsz). 2010;58:209-25.
  • Calder PC. Nutrition and immunity: lessons for COVID-19. Nutr Diabetes. 2021;11:19.
  • Kotchen TA, Cowley AW Jr, Frohlich ED. Salt in health and disease--a delicate balance. N Engl J Med. 2013;368:1229-37.
  • Yabas M, Bostanci A, Aral S. ATP11C promotes the differentiation of pre-B cells into immature B cells but does not affect their IL-7-dependent proliferation. Immunol Res. 2023;71:609-16.
  • Flemming A, Brummer T, Reth M, Jumaa H. The adaptor protein SLP-65 acts as a tumor suppressor that limits pre-B cell expansion. Nat Immunol. 2003;4:38-43.
  • Lindner SE, Lohmuller M, Kotkamp B, et al. The miR-15 family reinforces the transition from proliferation to differentiation in pre-B cells. EMBO Rep. 2017;18:1604-17.
  • Binger KJ, Gebhardt M, Heinig M, et al. High salt reduces the activation of IL-4- and IL-13-stimulated macrophages. J Clin Invest. 2015;125:4223-38.
  • Kleinewietfeld M, Manzel A, Titze J, et al. Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. Nature. 2013;496:518-22.
  • Wilck N, Matus MG, Kearney SM, et al. Salt-responsive gut commensal modulates TH17 axis and disease. Nature. 2017;551:585-9.
  • Hernandez AL, Kitz A, Wu C, et al. Sodium chloride inhibits the suppressive function of FOXP3+ regulatory T cells. J Clin Invest. 2015;125:4212-22.
  • Jobin K, Stumpf NE, Schwab S, et al. A high-salt diet compromises antibacterial neutrophil responses through hormonal perturbation. Sci Transl Med. 2020;12:eaay3850.
  • Machnik A, Neuhofer W, Jantsch J, et al. Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism. Nat Med. 2009;15:545-52.
  • Cvetkovic L, Perisic S, Titze J, et al. The impact of hyperosmolality on activation and differentiation of B lymphoid cells. Front Immunol. 2019;10:828.
  • Wiig H, Schroder A, Neuhofer W, et al. Immune cells control skin lymphatic electrolyte homeostasis and blood pressure. J Clin Invest. 2013;123:2803-15.
  • Quah BJ, Parish CR. New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes. J Immunol Methods. 2012;379:1-14.
  • He W, Xu J, Mu R, et al. High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation. Nat Commun. 2020;11:1732.
  • Tan M, He FJ, Wang C, MacGregor GA. Twenty-four-hour urinary sodium and potassium excretion in China: a systematic review and meta-analysis. J Am Heart Assoc. 2019;8:e012923.
  • Jobin K, Muller DN, Jantsch J, Kurts C. Sodium and its manifold impact on our immune system. Trends Immunol. 2021;42:469-79.
  • Toussirot E, Bereau M, Vauchy C, Saas P. Could sodium chloride be an environmental trigger for immune-mediated diseases? An overview of the experimental and clinical evidence. Front Physiol. 2018;9:440.
  • Wilck N, Balogh A, Marko L, et al. The role of sodium in modulating immune cell function. Nat Rev Nephrol. 2019;15:546-58.
  • Jantsch J, Schatz V, Friedrich D, et al. Cutaneous Na+ storage strengthens the antimicrobial barrier function of the skin and boosts macrophage-driven host defense. Cell Metab. 2015;21:493-501.
  • Zhang WC, Du LJ, Zheng XJ, et al. Elevated sodium chloride drives type I interferon signaling in macrophages and increases antiviral resistance. J Biol Chem. 2018;293:1030-9.
  • Willebrand R, Hamad I, Van Zeebroeck L, et al. High salt inhibits tumor growth by enhancing anti-tumor immunity. Front Immunol. 2019;10:1141.
  • Na SY, Janakiraman M, Leliavski A, Krishnamoorthy G. High-salt diet suppresses autoimmune demyelination by regulating the blood-brain barrier permeability. Proc Natl Acad Sci U S A. 2021;118:e2025944118.
  • Wu C, Yosef N, Thalhamer T, et al. Induction of pathogenic TH17 cells by inducible salt-sensing kinase SGK1. Nature. 2013;496:513-7.
  • Hucke S, Eschborn M, Liebmann M, et al. Sodium chloride promotes pro-inflammatory macrophage polarization thereby aggravating CNS autoimmunity. J Autoimmun. 2016;67:90-101.
  • Li X, Alu A, Wei Y, et al. The modulatory effect of high salt on immune cells and related diseases. Cell Prolif. 2022;55:e13250.
  • Kino T, Takatori H, Manoli I, et al. Brx mediates the response of lymphocytes to osmotic stress through the activation of NFAT5. Sci Signal. 2009;2:ra5.
  • Milne CD, Paige CJ. IL-7: a key regulator of B lymphopoiesis. Semin Immunol. 2006;18:20-30.
  • Corfe SA, Paige CJ. The many roles of IL-7 in B cell development; mediator of survival, proliferation and differentiation. Semin Immunol. 2012;24:198-208.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Temel İmmünoloji
Bölüm Özgün Makaleler
Yazarlar

Mehmet Yabaş 0000-0002-3462-5389

Yayımlanma Tarihi 19 Ekim 2023
Kabul Tarihi 28 Eylül 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 5 Sayı: Supplement (1) - Innovations in Medicine and Healthcare in the 100th Year of the Republic

Kaynak Göster

AMA Yabaş M. High Salt-Induced Hyperosmolality Reduces in Vitro Survival and Proliferation of Pre-B Cells. Med Records. Ekim 2023;5(Supplement (1):150-4. doi:10.37990/medr.1352143
 Kapak Resmi İndir

         

Chief Editors
Assoc. Prof. Zülal Öner
Address: İzmir Bakırçay University, Department of Anatomy, İzmir, Turkey

Assoc. Prof. Deniz Şenol
Address: Düzce University, Department of Anatomy, Düzce, Turkey

E-mail: medrecsjournal@gmail.com

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Medical Records Association (Tıbbi Kayıtlar Derneği)
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