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Evaluation of dynamic thiol-disulfide balance and ischemia modified albumin levels in patients with chronic kidney disease

Yıl 2022, Cilt: 13 Sayı: 47, 237 - 242, 15.12.2022
https://doi.org/10.17944/mkutfd.947113

Öz

Objective: In this study, it was aimed to determine the dynamic thiol-disulfide balance and ischemia modified albumin (IMA) levels in patients with chronic kidney disease (CKD).

Method: Thirty hemodialysis (HD), 30 CKD patients (stage 3-5) and 30 controls were included in the study. The dynamic thiol-disulfide balance was determined by the colorimetric method developed by Erel et al. IMA levels were determined by using cobalt binding test developed by Bar- Or et al.

Results: Native and total thiol levels of CKD and HD patients were significantly lower than that of the control group (p=0.001 for both). However, disulfide levels were significantly higher in the HD group (p=0.001), but there was no significant difference between control and CKD groups(p=0.547). A statistically significant negative correlation was found between the native and total thiol levels and IMA (r=-0.628; -0.631), BUN (r=-0.747; -0.747), and creatinine (r=-0.732; -0.721). There was a significant positive correlation between GFR and the thiol levels (r=0.835;0.824). TrxR levels were significantly higher in the patient groups compared to the controls (p=0.001). CRP levels of the patient groups were significantly higher compared to the controls (p=0.001).

Conclusion: We have demonstrated that measurement of dynamic thiol-disulfide levels by using colorimetric method can contribute to the diagnosis and follow-up of the disease as a marker, because it is easily applicable in routine clinical biochemistry laboratories and related with disease severity in CKD patients. Also, we showed that albumin correction due to dialysis process should be consider in studies dealing with plasma thiol values and the final results should be given after the correction process.

Destekleyen Kurum

Hatay Mustafa Kemal Üniversitesi

Proje Numarası

18.D.002

Teşekkür

This research was supported by Hatay Mustafa Kemal University Coordination Office of Scientific Research Projects (Project # 18.D.002). This research was supported by Hatay Mustafa Kemal University Coordination Office of Scientific Research Projects (Project # 18.D.002). This research was supported by Hatay Mustafa Kemal University Coordination Office of Scientific Research Projects

Kaynakça

  • Böger RH. The emerging role of asymmetric dimethylarginine as a novel cardiovascular risk factor. Cardiovasc Res 2003;59 (4): 824-33. https://doi.org/10.1016/s0008-6363(03)00500-5.
  • Sugimoto M, Yamaoka Y. Review of Helicobacter pylori infection and chronic renal failure. Therapeutic Ther Apher Dial. 2011;15(1):1-9. https://doi.org/10.1111/j.1744-9987.2010.00851.x.
  • Romagnani P, Remuzzi G, Glassock R, Levin A, Jager KJ, Tonelli M, et al. Chronic kidney disease. Nat Rev Dis Primers. 2017;3(1): 1-24. https://doi.org/10.1038/nrdp.2017.88.
  • Özcan O, Erdal H, Çakırca G, Yönden Z. Oksidatif stress ve hücre içi lipit, protein ve DNA yapıları üzerine etkileri. J Clin Exp Invest. 2015;6(3):331-6. https://doi.org/10.5799/ahinjs.01.2015.03.0545.
  • Liakopoulos V, Roumeliotis S, Gorny X, Dounousi E, Mertens PR. Oxidative stress in hemodialysis patients: a review of the literature. Oxid Med Cell Longev. 2017;2017: 3081856.https://doi.org/10.1155/2017/3081856.
  • Modaresi A, Nafar M, Sahraei Z. Oxidative stress in chronic kidney disease. Iran J Kidney Dis. 2015; 9(3):165 -79.
  • Zhang J, Li X, Han X, Liu R, Fang J. Targeting the thioredoxin system for cancer theraphy. Trends Pharmacol Sci. 2017;38(9): 794-808.https://doi.org/10.1016/j.tips.2017.06.001.
  • Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr. 2000;72(2):653-69. https://doi.org/10.1093/ajcn/72.2.653S.
  • Ates I, Ozkayar N, Inan B, Yılmaz FM, Topcuoğlu C, et al. Dynamic thiol/disulphide homeostasis in patients with newly diagnosed primary hypertension. J Am Soc Hypertens. 2016;10(2):159-66. https://doi.org/10.1016/j.jash.2015.12.008.
  • Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-32. https://doi.org/10.1016/j.clinbiochem.2014.09.026.
  • Williams CH, Arscott LD, Müller S, Lennon BW, Ludwig ML, Wang PF, et al. Thioredoxin reductase: two modes of catalysis have evolved. Eur J Biochem. 2000;267(20):6110-7. https://doi.org/10.1046/j.1432-1327.2000.01702.x.
  • Zagrodzki P. Thioredoxin reductase--a new target for molecular medical investigations. Postepy Hig Med Dosw. 2002;56(2):155-67.
  • Holmgren A. Thioredoxin and glutaredoxin systems. J Biol Chem. 1989;264 (24):139636. https://doi.org/10.1016/S0021-9258(18)71625-6.
  • Gromer S, Gross JH. Methylseleninate is a substrate rather than an inhibitor of mammalian thioredoxin reductase Implications for the antitumor effects of selenium. J Biol Chem. 2002;277(12):9701-6. https://doi.org/10.1074/jbc.M109234200.
  • Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-11. https://doi.org/ 10.1016/j.clinbiochem.2005.08.008.
  • Bar-Or D, Curtis G, Rao N, Bampos N, Lau E. Characterization of the Co(2+) and Ni(2+) binding amino-acid residues of the N-terminus of human albumin. An insight into the mechanism of a new assay for myocardial ischemia. Eur J Biochem. 2001;268(1):42-7. https://doi.org/10.1046/j.1432-1327.2001.01846.x.
  • Coşkun C, Emre HÖ, Gümüş A, Uzun S, Karadağ S, Behlül A, et al. Diyabetik ve Diyabetik Olmayan Kronik Böbrek Yetmezliğinde Dinamik Tiyol Disülfit Homeostazı ve İleri Protein Oksidasyon Ürünleri (AOPPs). Deneysel Tıp Araştırma Enstitüsü Dergisi.6(12):1-9.
  • Ateş İ, Özkayar N, Yılmaz FM, Bayrakçı N, Neşelioğlu S et al. Oxidative stress level in patients with chronic kidney disease. Ortadogu Medical Journal/Ortadogu Tıp Dergisi. 2018;10(1). https://doi.org/10.21601/ortadogutipdergisi.308443.
  • Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox rocesses. An Introduction to a Special Issue of Free Radical Biology and Medicine - “Reactive Oxygen Species and Musculoskeletal Aging”. McArdle A, Jackson MJ. Free Radic Biol Med. 2013; 65:244-53. https://doi.org/10.1016/j.freeradbiomed.2013.05.050.
  • Carbonell LF, Nadal JA, Llanos MC, Hernandez I, Nava E, Diaz J. Depletion of liver glutathione potentiates the oxidative stress and decreases nitric oxide synthesis in a rat endotoxin shock model. Crit Care Med. 2000; 28(6):2002-6. https://doi.org/10.1097/00003246-200006000-00054.
  • Ayar G, Sahin S, Yazici MU, Neselioglu S, Erel O, Bayrakcı US. Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury. BioMed Research International. 2018;2018. https://doi.org/10.1155/2018/1898671.
  • Fanti P, Giustarini D, Rossi R, Cunningham SE, Folli F, Khazim K, et al. Dietary intake of proteins and calories is inversely associated with the oxidation state of plasma thiols in end-stage renal disease patients. J Ren Nutr. 2015;25(6):494-503. https://doi.org/10.1053/j.jrn.2015.06.003.
  • Simons AL, Parsons AD, Foster KA, Orcutt KP, Fath MA, Spitz DR. Inhibition of glutathione and thioredoxin metabolism enhances sensitivity to perifosine in head and neck cancer cells. J Oncol. 2009;2009. https://doi.org/10.1155/2009/519563.
  • Otal Y, Dermican S, Sener A, Alisik M, Tanriverdi F, Güllü Ercan Haydar F. Acute renal failure and thiol-disulfide homeostasis. J Nephrol Ther. 2018;8: 312. https://doi.org/10.4172/2161-0959.1000312.
  • Turedi S, Cinar O, Yavuz I, Mentese A, Gunduz A, Karahan SC, et al. Differences in ischemia-modified albumin levels between end stage renal disease patients and the normal population. J Nephrol. 2010;23(3):335-40.

Kronik Böbrek Hastalığı Olan Hastalarda Dinamik Tiyol-Disülfid Dengesi ve İskemi Modifiye Albümin Düzeylerinin Değerlendirilmes

Yıl 2022, Cilt: 13 Sayı: 47, 237 - 242, 15.12.2022
https://doi.org/10.17944/mkutfd.947113

Öz

Amaç: Bu çalışmada Kronik böbrek hastalığı (KBH) olan hastalarda dinamik tiyol-disülfid dengesi ve iskemi modifiye albümin (IMA) düzeylerinin belirlenmesi amaçlanmıştır.

Yöntem: Çalışmaya 30 hemodiyaliz (HD), 30 KBH hastası (evre 3-5) ve 30 kontrol dahil edildi. Dinamik tiyol-disülfid dengesi, Erel ve arkadaşları tarafından geliştirilen kolorimetrik yöntemle belirlendi. IMA seviyeleri Bar- Or ve ark. tarafından geliştirilen kobalt bağlama testi kullanılarak belirlendi.

Bulgular: KBH ve HD hastalarının serbest ve toplam tiyol seviyeleri kontrol grubuna göre anlamlı derecede düşüktü (her ikisi için p=0.001). Ancak disülfid düzeyleri HD grubunda anlamlı olarak daha yüksekti (p=0.001), ancak kontrol ve KBH grupları arasında anlamlı fark yoktu (p=0.547). Serbest ve toplam tiyol seviyeleri ile IMA (r=-0.628;-0.631), BUN (r=-0.747;-0.747) ve kreatinin (r=-0.732;-0.721) arasında istatistiksel olarak anlamlı bir negatif korelasyon bulundu. GFR ile tiyol düzeyleri arasında anlamlı pozitif korelasyon vardı (r=0.835;0.824). Hasta gruplarında TrxR düzeyleri kontrollere göre anlamlı derecede yüksekti (p=0.001). Hasta gruplarının CRP düzeyleri kontrollere göre anlamlı derecede yüksekti (p=0.001).

Sonuç: Dinamik tiyol-disülfid düzeylerinin kolorimetrik yöntem kullanılarak ölçülmesinin, rutin klinik biyokimya laboratuvarlarında kolaylıkla uygulanabilmesi ve KBH hastalarında hastalık şiddeti ile ilişkili olması nedeniyle bir belirteç olarak hastalığın tanı ve takibine katkı sağlayabileceğini gösterdik. Ayrıca plazma tiyol değerleri ile ilgili çalışmalarda diyaliz sürecine bağlı albümin düzeltmesinin dikkate alınması gerektiğini ve düzeltme işleminden sonra nihai sonuçların verilmesi gerektiğini gösterdik.

Proje Numarası

18.D.002

Kaynakça

  • Böger RH. The emerging role of asymmetric dimethylarginine as a novel cardiovascular risk factor. Cardiovasc Res 2003;59 (4): 824-33. https://doi.org/10.1016/s0008-6363(03)00500-5.
  • Sugimoto M, Yamaoka Y. Review of Helicobacter pylori infection and chronic renal failure. Therapeutic Ther Apher Dial. 2011;15(1):1-9. https://doi.org/10.1111/j.1744-9987.2010.00851.x.
  • Romagnani P, Remuzzi G, Glassock R, Levin A, Jager KJ, Tonelli M, et al. Chronic kidney disease. Nat Rev Dis Primers. 2017;3(1): 1-24. https://doi.org/10.1038/nrdp.2017.88.
  • Özcan O, Erdal H, Çakırca G, Yönden Z. Oksidatif stress ve hücre içi lipit, protein ve DNA yapıları üzerine etkileri. J Clin Exp Invest. 2015;6(3):331-6. https://doi.org/10.5799/ahinjs.01.2015.03.0545.
  • Liakopoulos V, Roumeliotis S, Gorny X, Dounousi E, Mertens PR. Oxidative stress in hemodialysis patients: a review of the literature. Oxid Med Cell Longev. 2017;2017: 3081856.https://doi.org/10.1155/2017/3081856.
  • Modaresi A, Nafar M, Sahraei Z. Oxidative stress in chronic kidney disease. Iran J Kidney Dis. 2015; 9(3):165 -79.
  • Zhang J, Li X, Han X, Liu R, Fang J. Targeting the thioredoxin system for cancer theraphy. Trends Pharmacol Sci. 2017;38(9): 794-808.https://doi.org/10.1016/j.tips.2017.06.001.
  • Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr. 2000;72(2):653-69. https://doi.org/10.1093/ajcn/72.2.653S.
  • Ates I, Ozkayar N, Inan B, Yılmaz FM, Topcuoğlu C, et al. Dynamic thiol/disulphide homeostasis in patients with newly diagnosed primary hypertension. J Am Soc Hypertens. 2016;10(2):159-66. https://doi.org/10.1016/j.jash.2015.12.008.
  • Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-32. https://doi.org/10.1016/j.clinbiochem.2014.09.026.
  • Williams CH, Arscott LD, Müller S, Lennon BW, Ludwig ML, Wang PF, et al. Thioredoxin reductase: two modes of catalysis have evolved. Eur J Biochem. 2000;267(20):6110-7. https://doi.org/10.1046/j.1432-1327.2000.01702.x.
  • Zagrodzki P. Thioredoxin reductase--a new target for molecular medical investigations. Postepy Hig Med Dosw. 2002;56(2):155-67.
  • Holmgren A. Thioredoxin and glutaredoxin systems. J Biol Chem. 1989;264 (24):139636. https://doi.org/10.1016/S0021-9258(18)71625-6.
  • Gromer S, Gross JH. Methylseleninate is a substrate rather than an inhibitor of mammalian thioredoxin reductase Implications for the antitumor effects of selenium. J Biol Chem. 2002;277(12):9701-6. https://doi.org/10.1074/jbc.M109234200.
  • Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-11. https://doi.org/ 10.1016/j.clinbiochem.2005.08.008.
  • Bar-Or D, Curtis G, Rao N, Bampos N, Lau E. Characterization of the Co(2+) and Ni(2+) binding amino-acid residues of the N-terminus of human albumin. An insight into the mechanism of a new assay for myocardial ischemia. Eur J Biochem. 2001;268(1):42-7. https://doi.org/10.1046/j.1432-1327.2001.01846.x.
  • Coşkun C, Emre HÖ, Gümüş A, Uzun S, Karadağ S, Behlül A, et al. Diyabetik ve Diyabetik Olmayan Kronik Böbrek Yetmezliğinde Dinamik Tiyol Disülfit Homeostazı ve İleri Protein Oksidasyon Ürünleri (AOPPs). Deneysel Tıp Araştırma Enstitüsü Dergisi.6(12):1-9.
  • Ateş İ, Özkayar N, Yılmaz FM, Bayrakçı N, Neşelioğlu S et al. Oxidative stress level in patients with chronic kidney disease. Ortadogu Medical Journal/Ortadogu Tıp Dergisi. 2018;10(1). https://doi.org/10.21601/ortadogutipdergisi.308443.
  • Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox rocesses. An Introduction to a Special Issue of Free Radical Biology and Medicine - “Reactive Oxygen Species and Musculoskeletal Aging”. McArdle A, Jackson MJ. Free Radic Biol Med. 2013; 65:244-53. https://doi.org/10.1016/j.freeradbiomed.2013.05.050.
  • Carbonell LF, Nadal JA, Llanos MC, Hernandez I, Nava E, Diaz J. Depletion of liver glutathione potentiates the oxidative stress and decreases nitric oxide synthesis in a rat endotoxin shock model. Crit Care Med. 2000; 28(6):2002-6. https://doi.org/10.1097/00003246-200006000-00054.
  • Ayar G, Sahin S, Yazici MU, Neselioglu S, Erel O, Bayrakcı US. Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury. BioMed Research International. 2018;2018. https://doi.org/10.1155/2018/1898671.
  • Fanti P, Giustarini D, Rossi R, Cunningham SE, Folli F, Khazim K, et al. Dietary intake of proteins and calories is inversely associated with the oxidation state of plasma thiols in end-stage renal disease patients. J Ren Nutr. 2015;25(6):494-503. https://doi.org/10.1053/j.jrn.2015.06.003.
  • Simons AL, Parsons AD, Foster KA, Orcutt KP, Fath MA, Spitz DR. Inhibition of glutathione and thioredoxin metabolism enhances sensitivity to perifosine in head and neck cancer cells. J Oncol. 2009;2009. https://doi.org/10.1155/2009/519563.
  • Otal Y, Dermican S, Sener A, Alisik M, Tanriverdi F, Güllü Ercan Haydar F. Acute renal failure and thiol-disulfide homeostasis. J Nephrol Ther. 2018;8: 312. https://doi.org/10.4172/2161-0959.1000312.
  • Turedi S, Cinar O, Yavuz I, Mentese A, Gunduz A, Karahan SC, et al. Differences in ischemia-modified albumin levels between end stage renal disease patients and the normal population. J Nephrol. 2010;23(3):335-40.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Original Articles
Yazarlar

Huseyin Erdal 0000-0003-0786-5077

Oğuzhan Özcan 0000-0001-7486-503X

Faruk Hilmi Turgut 0000-0003-1910-7433

Salim Neşelioğlu 0000-0002-0974-5717

Özcan Erel 0000-0002-2996-3236

Proje Numarası 18.D.002
Yayımlanma Tarihi 15 Aralık 2022
Gönderilme Tarihi 3 Haziran 2021
Kabul Tarihi 13 Haziran 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 13 Sayı: 47

Kaynak Göster

Vancouver Erdal H, Özcan O, Turgut FH, Neşelioğlu S, Erel Ö. Evaluation of dynamic thiol-disulfide balance and ischemia modified albumin levels in patients with chronic kidney disease. mkutfd. 2022;13(47):237-42.