Investigation of the Protective Effects of Capparis Spinosa Extract in Indomethacin Induced Ulcer Model in Rats
Öz
ABSTRACT
Objective: Capparis spinosa is a genus of the Capparaceae family. In the literature, it has been found that the main components of Capparis buds, quercetin and kaempferol were found to be effective in many diseases. In this study, the protective effects of Capparis spinosa on damaged rat stomach tissue induced by indomethacin and some antioxidant parameters were investigated.
Material and Methods: A total of 36 female Sprague Dawley rats weighing 200-220 grams were used in the study and six groups were formed. Groups were: healthy group; positive control group (famotidine 20 mg/kg + indomethacin 25 mg/kg); negative control group (distilled water + indomethacin); low-dose study group (125 mg/kg Capparis spinosa + indomethacin); medium dose group (250 mg/kg Capparis spinosa + indomethacin); and high dose group (500 mg/kg Capparis spinosa + indomethacin). Six hours after indomethacin was given to the groups by gastric lavage, all rats were killed under general anesthesia. The stomachs of all rats were removed, the ulcerated areas on the stomach surface were evaluated macroscopically, and the ulcer areas were measured on mm2 paper. In addition, blood and stomach tissues of all rats were biochemically examined, and malondialdehyde, superoxide dismutase and glutathione parameters were measured. The antiulcer activity of CS was compared with all groups.
Results: When the ulcer area and histopathological evaluation were examined, it was determined that the group applied 250 mg/kg Capparis spinosa had an appearance close to the healthy group. It was also found that plant extracts at all concentrations decreased the level of MDA in rat gastric tissue and increased SOD activity and GSH levels statistically.
Conclusion: It was obtained that Capparis spinosa has antiulcer activity.
Anahtar Kelimeler
Destekleyen Kurum
This study was supported by Atatürk University Scientific Research Projects (BAP) coordination unit (THD-2018-6630) and Council of Higher Education (YOK, MEV-2017-162).
Proje Numarası
THD-2018-6630, YOK, MEV-2017-162
Kaynakça
- 1. Süleyman H, Demirezer LO, Büyükokuroglu ME, et al. Antiulcerogenic effect of Hippophae rhamnoides L. Phytother Res. 2001;15(7):625-7.
- 2. Soll AH. Peptic ulcer and its complications. In Feldman M, Scharschmidt B, Slesenger MH. Eds. SlesengerFordtran’s Gastrointestinal and Hepatic Disease: Pathophisiology, Diagnosis, Management. 6th ed. Philadelphia:Saunders, 1998; 620-78.
- 3. FriedmanLS, Peterson WL. Peptic ulcer and related disorders. In Fauci AS, Braunwald E, Isselbacher KJ, Wilson JD, Martin JB, Kasper DL, Hauser SL, Longo DL eds. Harrison’s Principles of Internal Medicine. 14th Ed. New York, Mc Graw-Hill, 1998; 1596-1616.
- 4. Soll AH, Isenberg J. Peptic ulcer disease: epidemiology, pathophisiology, clinical manifestations and diagnosis..In Drazen JM, Gill GN, Griggs RC, Kokko JP, Mandel GL, Powell DW, Schafer AI. eds. Goldman Bennett Cecil Texbook of Medicine. 21st Ed. Philadelphia, Saunders, 2000 671-5.
- 5. Hooderwerf WA, Pasricha PJ. Pharmacotherapy of gastric acidity, peptic ulcers, and gastroesophageal reflux disease. In Brunton L, editor. Goodman and Gilman’s the pharmacological basis of therapeutics, New-York: Mc Graw-Hill, 2006. p. 967-981.
- 6. Feldman F, Friedman LS, Sleisenger MH. Sleisenger and Fordtran’s Gastrointestinal and Liver Disease. Philadelphia: WB Saunders Co, p. 615.
- 7. Salim AS. Scavenging free radicals to prevent stress induced gastric mucosal injury. Lancet 1989; 1390.
- 8. Cadirci E, Suleyman H, Aksoy H, et al. Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats. Chem Biol Interact 2007; 170: 40-48.
- 9. Inocencio C, Rivera D, Alcaraz F,Tomas-Barberan FA. Flavonoid content of commercial capers (Capparis spinosa, C. sicula and C. orientalis) produced in Mediterranean countries. European Journal of Food Research and Technology. 2000; 212: 70-74.
- 10. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
- 11. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;25(1):192-205.
- 12. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.
- 13. Bayir Y, Karagoz Y, Karakus E, et al. Nigella sativa reduces tissue damage in rat ovaries subjected to torsion and detorsion: oxidative stress, proinflammatory response and histopathological evaluation. Gynecol Obstet Invest. 2012;74(1):41-9.
- 14. Zhang H, Ma ZF. Phytochemical and Pharmacological Properties of Capparis spinosa as a Medicinal Plant. Nutrients. 2018;10(2):116.
- 15. Nabavi SF, Maggi F, Daglia M, Habtemariam S, Rastrelli L, Nabavi SM. Pharmacological Effects of Capparis spinosa L. Phytother Res. 2016;30(11):1733-1744.
- 16. Assadi S, Shafiee SM, Erfani M, Akmali M. Antioxidative and antidiabetic effects of Capparis spinosa fruit extract on high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats. Biomed Pharmacother. 202;138:111391. 17. Al-Anazi KM, Al-Mareed AA, Farah MA, Ali MA, Hailan WAQ, Al-Hemaid FM. Protective Effect of Capparis spinosa Extract against Potassium Bromate Induced Oxidative Stress and Genotoxicity in Mice. Evid Based Complement Alternat Med. 2021;8875238.
- 18. Akbari R, Yaghooti H, Jalali MT, Khorsandi LS, Mohammadtaghvaei N. Capparis spinosa improves the high fat diet-induced non-alcoholic steatohepatitis in rats: the possible role of FGF21. BMC Res Notes. 2020;13(1):356.
- 19. Rakhshandeh H, Rashidi R, Vahedi MM, Khorrami MB, Abbassian H, Forouzanfar F. Hypnotic Activity of Capparis spinosa Hydro-alcoholic Extract in Mice. Recent Pat Food Nutr Agric. 2021;12(1):58-62.
- 20. Mahmoudvand H, Khalaf AK, Beyranvand M. In Vitro and Ex Vivo Evaluation of Capparis Spinosa Extract to Inactivate Protoscoleces During Hydatid cyst Surgery. Curr Drug Discov Technol. 2021;18(5):e18082020185049.
Öz
Proje Numarası
THD-2018-6630, YOK, MEV-2017-162
Kaynakça
- 1. Süleyman H, Demirezer LO, Büyükokuroglu ME, et al. Antiulcerogenic effect of Hippophae rhamnoides L. Phytother Res. 2001;15(7):625-7.
- 2. Soll AH. Peptic ulcer and its complications. In Feldman M, Scharschmidt B, Slesenger MH. Eds. SlesengerFordtran’s Gastrointestinal and Hepatic Disease: Pathophisiology, Diagnosis, Management. 6th ed. Philadelphia:Saunders, 1998; 620-78.
- 3. FriedmanLS, Peterson WL. Peptic ulcer and related disorders. In Fauci AS, Braunwald E, Isselbacher KJ, Wilson JD, Martin JB, Kasper DL, Hauser SL, Longo DL eds. Harrison’s Principles of Internal Medicine. 14th Ed. New York, Mc Graw-Hill, 1998; 1596-1616.
- 4. Soll AH, Isenberg J. Peptic ulcer disease: epidemiology, pathophisiology, clinical manifestations and diagnosis..In Drazen JM, Gill GN, Griggs RC, Kokko JP, Mandel GL, Powell DW, Schafer AI. eds. Goldman Bennett Cecil Texbook of Medicine. 21st Ed. Philadelphia, Saunders, 2000 671-5.
- 5. Hooderwerf WA, Pasricha PJ. Pharmacotherapy of gastric acidity, peptic ulcers, and gastroesophageal reflux disease. In Brunton L, editor. Goodman and Gilman’s the pharmacological basis of therapeutics, New-York: Mc Graw-Hill, 2006. p. 967-981.
- 6. Feldman F, Friedman LS, Sleisenger MH. Sleisenger and Fordtran’s Gastrointestinal and Liver Disease. Philadelphia: WB Saunders Co, p. 615.
- 7. Salim AS. Scavenging free radicals to prevent stress induced gastric mucosal injury. Lancet 1989; 1390.
- 8. Cadirci E, Suleyman H, Aksoy H, et al. Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats. Chem Biol Interact 2007; 170: 40-48.
- 9. Inocencio C, Rivera D, Alcaraz F,Tomas-Barberan FA. Flavonoid content of commercial capers (Capparis spinosa, C. sicula and C. orientalis) produced in Mediterranean countries. European Journal of Food Research and Technology. 2000; 212: 70-74.
- 10. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
- 11. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;25(1):192-205.
- 12. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.
- 13. Bayir Y, Karagoz Y, Karakus E, et al. Nigella sativa reduces tissue damage in rat ovaries subjected to torsion and detorsion: oxidative stress, proinflammatory response and histopathological evaluation. Gynecol Obstet Invest. 2012;74(1):41-9.
- 14. Zhang H, Ma ZF. Phytochemical and Pharmacological Properties of Capparis spinosa as a Medicinal Plant. Nutrients. 2018;10(2):116.
- 15. Nabavi SF, Maggi F, Daglia M, Habtemariam S, Rastrelli L, Nabavi SM. Pharmacological Effects of Capparis spinosa L. Phytother Res. 2016;30(11):1733-1744.
- 16. Assadi S, Shafiee SM, Erfani M, Akmali M. Antioxidative and antidiabetic effects of Capparis spinosa fruit extract on high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats. Biomed Pharmacother. 202;138:111391. 17. Al-Anazi KM, Al-Mareed AA, Farah MA, Ali MA, Hailan WAQ, Al-Hemaid FM. Protective Effect of Capparis spinosa Extract against Potassium Bromate Induced Oxidative Stress and Genotoxicity in Mice. Evid Based Complement Alternat Med. 2021;8875238.
- 18. Akbari R, Yaghooti H, Jalali MT, Khorsandi LS, Mohammadtaghvaei N. Capparis spinosa improves the high fat diet-induced non-alcoholic steatohepatitis in rats: the possible role of FGF21. BMC Res Notes. 2020;13(1):356.
- 19. Rakhshandeh H, Rashidi R, Vahedi MM, Khorrami MB, Abbassian H, Forouzanfar F. Hypnotic Activity of Capparis spinosa Hydro-alcoholic Extract in Mice. Recent Pat Food Nutr Agric. 2021;12(1):58-62.
- 20. Mahmoudvand H, Khalaf AK, Beyranvand M. In Vitro and Ex Vivo Evaluation of Capparis Spinosa Extract to Inactivate Protoscoleces During Hydatid cyst Surgery. Curr Drug Discov Technol. 2021;18(5):e18082020185049.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Gastroenteroloji ve Hepatoloji |
| Bölüm | Research Articles |
| Yazarlar | |
| Proje Numarası | THD-2018-6630, YOK, MEV-2017-162 |
| Yayımlanma Tarihi | 30 Ocak 2024 |
| Gönderilme Tarihi | 12 Eylül 2022 |
| Yayımlandığı Sayı | Yıl 2024 Cilt: 5 Sayı: 1 |
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