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IN VITRO SPERMATOLOGICAL PARAMETERS IN DRONES

Yıl 2023, Cilt: 23 Sayı: 2, 268 - 279, 19.11.2023
https://doi.org/10.31467/uluaricilik.1279779

Öz

Honey bees are an indispensable element of the ecosystem, as they provide an important part of plant pollination beyond food production. The queen bee, which forms the core of the bee colony, is at the center of bee production in the beekeeping sector. Undoubtedly, the production of high-quality queen bees primarily depends on suitable drones and therefore, quality sperm. In addition, artificial insemination of queen bees is a successful production method, as in other species, due to its superior characteristics compared to natural mating. In many apiaries and research centers, artificial insemination is used routinely for queen production. One of the advantages of this method is that the sperm can be preserved for the short or long term. In this respect, it is very effective in protecting genetic resources, preventing bee diseases and facilitating bee transportation. Today, spermatological studies and parameters used in drones are very few compared to other species. With the increasing importance given to the subject in recent years, many spermatological parameters have started to be used in honey bees. However, the morphological and physiological differences of drone semen require the development and standardization of these parameters with further studies. In this review, the spermatological parameters used in drone semen, together with the working principles and materials used, were examined in general terms and presented to the readers.

Kaynakça

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Bal Arılarında İn Vitro Spermatolojik Parametreler

Yıl 2023, Cilt: 23 Sayı: 2, 268 - 279, 19.11.2023
https://doi.org/10.31467/uluaricilik.1279779

Öz

Bal arıları, gıda üretiminin ötesinde bitki tozlaşmasının önemli bir bölümünü sağladıkları için ekosistemin vazgeçilmez bir unsurudur. Arı kolonisinin çekirdeğini oluşturan ana arı, arıcılık sektöründe arı üretiminin merkezinde yer almaktadır. Kuşkusuz kaliteli ana arı üretimi öncelikle uygun erkek arılara ve dolayısıyla kaliteli spermaya bağlıdır. Ayrıca suni tohumlama doğal çiftleşmeye göre üstün özellikleri nedeniyle diğer türlerde olduğu gibi başarılı bir üretim yöntemidir. Birçok arıcılık işletmesinde ve araştırma merkezinde ana arı üretimi için suni tohumlama rutin olarak kullanılmaktadır. Bu yöntemin avantajlarından biri de spermanın kısa ya da uzun süreli saklanabilmesidir. Bu yönüyle genetik kaynakların korunmasında, arı hastalıklarının önlenmesinde ve arı taşımacılığının kolaylaştırılmasında oldukça etkilidir. Günümüzde erkek arılarda kullanılan spermatolojik çalışmalar ve uygulanan parametreler diğer türlere göre çok azdır. Son yıllarda konuya verilen önemin artmasıyla bal arılarında birçok spermatolojik parametre kullanılmaya başlanmıştır. Ancak erkek arı spermasının morfolojik ve fizyolojik farklılıkları, bu parametrelerin ileri çalışmalarla geliştirilmesini ve standardize edilmesini gerektirmektedir. Bu derlemede erkek arı spermasında kullanılan spermatolojik parametreler, çalışma prensipleri ve kullanılan materyaller genel hatlarıyla incelenmiş ve okuyucuların beğenisine sunulmuştur.

Kaynakça

  • Abdelkader F, Kairo G, Bonnet M, Barbouche N, Belzunces LP et al. Effects of Clothianidin on Antioxidant Enzyme Activities and Malondialdehyde Level in Honey Bee Drone Semen. J. Apic. Res. 2019;58 (5):740-745. http://dx.doi.org/10.1080/00218839.2019.1655182
  • Abdelkader F, Kairo G, Tchamitchian S, Cousin M, Senechal J et al. Semen Quality of Honey Bee Drones Maintained from Emergence to Sexual Maturity Under Laboratory, Semi-Field and Field Conditions. Apidologie, 2014;45(2):215-223. http://dx.doi.org/10.1007/s13592-013-0240-7.
  • Alcay S, Cakmak S, Cakmak I, Aktar A, Yilmaz M et al. L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa. Kafkas Univ. Vet. Fak. Derg. 2021;27(4):489-493. http://10.9775/kvfd.2021.25756
  • Alçay S, Çakmak S, Cakmak I, Aktar A, Yılmaz M et al. Honey Bee Drone (Apis mellifera) Sperm Cryopreservation with Rainbow Trout Seminal Plasma Supplemented Extenders. J. Hell. Vet. Medical Soc. 2022;1(73):3745-3750. http://dx.doi.org/10.12681/jhvms.25651
  • Alcay S, Cakmak S, Cakmak I, Mulkpinar E, Gokce E et al. Cryobiology Successful Cryopreservation of Honey Bee Drone Spermatozoa with Royal Jelly Supplemented Extenders. Cryobiology, 2019;87(October 2018):28-31. https://doi.org/10.1016/j.cryobiol.2019.03.005.
  • Alçay S, Çakmak S, Çakmak İ, Mülkpinar E, Toker MB et al. Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Univ. Vet. Fak. Derg. 2019;25(4):553-557. htttp://10.9775/kvfd.2018.2131.
  • Andere CI, Monteavaro C, Palacio MA, Catena M, Rodríguez EM et al. Apis mellifera Semen: Bacterial Contamination and Susceptibility to Antibiotics. Apidologie 2011;42(5):551-559. http://dx.doi.org/10.1007/s13592-011-0051-7
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  • Lodesani M, Balduzzi D, Galli A. Functional Characterisation of Semen in Honeybee Queen (A m ligustica) Spermatheca and Efficiency of The Diluted Semen Technique in Instrumental Insemination. Ital. J. Anim. Sci. 2004;3(4):385-392. https://doi.org/10.4081/ijas.2004.385
  • Mackensen O. Viability and Sex Determination in The Honey Bee (Apis mellifera L.). Genetics 1951;36(5):500. http://dx.doi.org/10.1093/genetics/36.5.500
  • Morais LS, Araujo Neto ER, Silva AM, Marinho DEL, Bezerra LGP et al. Sperm Characteristics of Africanized Honey Bee (Apis mellifera L.) Drones During Dry and Wet Seasons in The Caatinga Biome. J. Apic. Res. 2022;1-8. http://dx.doi.org/10.1080/00218839.2022.2113328
  • Moreira DR, de Souza THS, Galhardo D, Puentes SMD, Figueira CL et al. Imidacloprid Induces Histopathological Damage in The Midgut, Ovary, and Spermathecal Stored Spermatozoa of Queens After Chronic Colony Exposure. Environ. Toxicol. Chem. 2022;41 (7):1637-1648. http://dx.doi.org/10.1002/etc.5332
  • Moritz RFA. Der Einfluss der Inzucht auf die Fitness der Drohnen von Apis mellifera carnica. Apidologie, 1981;12(1):41-55. http://dx.doi.org/10.1051/apido:19810104
  • Moritz RFA. The Effect of Different Diluents on Insemination Success in The Honeybee Using Mixed Semen. J. Apic. Res. 1984;23(3):164-167. http://dx.doi.org/10.1080/00218839.1984.11100626
  • Muller K, Pomorski T, Muller P, Herrmann A. Stability of Transbilayer Phospholipid Asymmetry in Viable Ram Sperm Cells After Cryotreatment. J. Cell. Sci.1999;112(1):11-20. http://dx.doi.org/10.1242/jcs.112.1.11
  • Murray JF, van der Horst G, Allsopp M, Kotzé RCM. A New Fluorescent Method to Determine Honey Bee Sperm Motility Parameters with Computer-Aided Sperm Analysis. J. Apic. Res. 2022;62(4):944-952. http://dx.doi.org/10.1080/00218839.2022.2090729
  • Papa G, Maier R, Durazzo A, Lucarini M, Karabagias IK et al. The Honey Bee Apis mellifera: An Insect at The Interface Between Human and Ecosystem Health. Biology (Basel), 2022;11 (2):233. http://dx.doi.org/10.3390/biology11020233
  • Peña FJ, Johannisson A, Wallgren M, Rodrı́guez-Martı́nez H. Assessment of Fresh and Frozen–Thawed Boar Semen Using An Annexin-V Assay: A New Method of Evaluating Sperm Membrane Integrity. Theriogenology, 2003;60(4):677-689. https://doi.org/10.1016/S0093-691X(03)00081-5
  • Peng Y, Locke SJ, Nasr ME, Liu TP, Montague MA. Differential Staining for Live and Dead Sperm of Honey Bees. Physiol. Entomol. 1990;15 (2):211-217. http://dx.doi.org/10.1111/j.1365-3032.1990.tb00509.x
  • Power K, D’Anza E, Martano M, Albarella S, Ciotola F et al. Morphological and Morphometric Analysis of The Italian Honeybee (Apis mellifera ligustica) Spermatozoa: A Preliminary Study in Campania Region. Vet. Med. Sci. 2019;6(2):0-61. http://dx.doi.org/10.7243/2054-3425-6-2
  • Rajamohan A, Danka RG, Hopkins BK, Rinehart JP. A Non-Activating Diluent to Prolong in Vitro Viability of Apis mellifera Spermatozoa: Effects on Cryopreservation and on Egg Fertilization. Cryobiology 2020;92(September 2019):124–129. http://dx.doi.org/10.1016/j.cryobiol.2019.11.045
  • Rhodes JW, Harden S, Spooner-Hart R, Anderson DL, Wheen G. Effects of Age, Season and Genetics on Semen and Sperm Production in Apis mellifera Drones. Apidologie, 2011;42 (1):29-38. http://dx.doi.org/10.1051/apido/2010026
  • Rinderer TE, Collins AM, Pesante D, Daniel R, Lancaster V et al. Comparison of Africanized and European Drones: Weights, Mucus Gland and Seminal Vesicle Weights, and Counts of Spermatozoa. Apidologie, 1985;16(4):407-412. http://dx.doi.org/10.1051/apido:19850405
  • Rinderer TE, De Guzman LI, Lancaster VA, Delatte GT, Stelzer JA. Varroa in The Mating Yard: I. The Effects of Varroa Jacobsoni and Apistan on Drone Honey Bees. Am. Bee J. 1999;139:134-139. 134–139
  • Rousseau A, Fournier V, Giovenazzo P. Apis mellifera (Hymenoptera: Apidae) Drone Sperm Quality in Relation to Age, Genetic Line, and Time of Breeding. Can. Entomol. 2015;147(6):702-711. http://dx.doi.org/10.4039/tce.2015.12
  • Rousseau A, Giovenazzo P. Optimizing Drone Fertility with Spring Nutritional Supplements to Honey Bee (Hymenoptera: Apidae) Colonies. J. Econ. Entomol. 2016;109(3):1009-1014. http://dx.doi.org/10.1093/jee/tow056
  • Rowland SC, Jacobson JD, Patton WC, King A, Chan PJ. Dual Fluorescence Analysis of DNA Apoptosis in Sperm. Am. J. Obstet. Gynecol. 2003;188(5):1156-1157. http://dx.doi.org/10.1067/mob.2003.295
  • Schlüns H, Schlüns EA, Van Praagh J, Moritz RFA. Sperm Numbers in Drone Honeybees (Apis mellifera) Depend on Body Size. Apidologie, 2003;34(6):577-584. http://dx.doi.org/10.1051/apido:2003051
  • Sharma GP, Gupta BL, Kumbkarni CG. Cytology of Spermatogenesis in The Honey Bee, Apis indica (F.). J. R. Microsc. Soc. 1961;79(4):337-351. http://dx.doi.org/10.1111/j.1365-2818.1961.tb05227.x
  • Snodgrass RE. Anatomy of The Honey Bee. Cornell University Press. 1956, p.130-134
  • Stürup M, Baer-Imhoof B, Nash DR, Boomsma JJ, Baer B. When Every Sperm Counts: Factors Affecting Male Fertility in The Honeybee Apis mellifera. Behav. Ecol. 2013;24(5):1192-1198. http://dx.doi.org/10.1093/beheco/art049
  • Taylor MA, Guzmán-Novoa E, Morfin N, Buhr MM. Improving Viability of Cryopreserved Honey Bee (Apis mellifera L.) Sperm with Selected Diluents, Cryoprotectants and Semen Dilution Ratios. Theriogenology, 2009;72(2):149-159. https://doi.org/10.1016/j.theriogenology.2009.02.012
  • Tofilski A. A Scientific Note on Amoeboid Movement of Honey Bee Semen. Apidologie, 2014;45:637–640. http://dx.doi.org/10.1007/s13592-014-0269-2
  • Uysal O, Bucak MN. The Role of Different Trehalose Concentrations and Cooling Rates in Freezing of Ram Semen. Ankara Univ. Vet. Fak. Derg. 2009;56(2):99-103. https://doi.org/10.1501/Vetfak_0000002176
  • Wegener J, Bienefeld K. Toxicity of Cryoprotectants to Honey Bee Semen and Queens. Theriogenology, 2012;77(3):600-607. http://dx.doi.org/10.1016/j.theriogenology.2011.08.036
  • Wegener J, May T, Bienefeld K, Kamp N. New Methods and Media for The Centrifugation of Honey Bee (Hymenoptera: Apidae) Drone Semen. J. Econ. Entomol. 2014;107(1):47-53. http://dx.doi.org/10.1603/EC13159
  • Wegener J, May T, Kamp G, Bienefeld K. A Successful New Approach to Honeybee Semen Cryopreservation. Cryobiology 2014;69(2):236-242. http://dx.doi.org/10.1016/j.cryobiol.2014.07.011
  • Wegener J, May T, Knollmann U, Kamp G, Müller K, Bienefeld K. In Vivo Validation of In Vitro Quality Tests for Cryopreserved Honey Bee Semen. Cryobiology, 2012;65(2):126-131. http://dx.doi.org/10.1016/j.cryobiol.2012.04.010
  • Woyke J. Naturalne I Sztuczne Unasienianie Matek Pszczelich. Pszczelnicze Zeszyty Naukowe, 1960;4(3/4):183-275
  • Woyke J. Natural and Artificial Insemination of Queen Honeybees. Bee World, 1962;43(1):21-25. http://dx.doi.org/10.1080/0005772X.1962.11096922
  • Woyke J. Correlations Between The Age at Which Honeybee Brood was Grafted, Characteristics of The Resultant Queens, and Results of Insemination. J. Apic. Res. 1971;10(1):45-55. http://dx.doi.org/10.1080/00218839.1971.11099669
  • Woyke J. Ultrastructure of Single and Multiple Diploid Honeybee Spermatozoa. J. Apic. Res. 1984;23(3):123-135. http://dx.doi.org/10.1080/00218839.1984.11100621
  • Yániz JL, Silvestre MA, Santolaria P. Sperm Quality Assessment in Honey Bee Drones. Biology (Basel), 2020;9(7):1-16. http://dx.doi.org/10.3390/biology9070174
  • Yániz J, Palacín I, Santolaria P. Effect of Chamber Characteristics, Incubation, and Diluent on Motility of Honey Bee (Apis mellifera) Drone Sperm. Apidologie, 2019;50(4):472-481. http://dx.doi.org/10.1007/s13592-019-00659-y
  • Yu W-H, Li Z-H, Yang W-M, Li G-Z, Zhou X-M et al. Comparison Between Two Methods for Staining DNA of Apoptotic Spermatozoa. Zhonghua nan ke xue = National Journal of Andrology, 2005;11 (2):101-103
  • Yue C, Schröder M, Bienefeld K, Genersch E. Detection of Viral Sequences in Semen of Honeybees (Apis mellifera): Evidence for Vertical Transmission of Viruses Through Drones. J. Invertebr. Pathol. 2006;92 (2):105-108. http://dx.doi.org/10.1016/j.jip.2006.03.001
Toplam 78 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Entomoloji, Ziraat Mühendisliği (Diğer), Veteriner Cerrahi
Bölüm Derlemeler
Yazarlar

Abdulkadir Kaya 0000-0001-7903-4358

Ongun Uysal 0000-0001-5025-6512

Erken Görünüm Tarihi 15 Kasım 2023
Yayımlanma Tarihi 19 Kasım 2023
Kabul Tarihi 18 Mayıs 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 23 Sayı: 2

Kaynak Göster

Vancouver Kaya A, Uysal O. IN VITRO SPERMATOLOGICAL PARAMETERS IN DRONES. U.Arı D.-U.Bee J. 2023;23(2):268-79.

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