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Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves

Yıl 2016, Cilt: 35 Sayı: 1-2, 7 - 10, 12.12.2016
https://doi.org/10.30782/uluvfd.393909

Öz

During routine diagnostic investigations on submitted serum and swab samples from three (n:3) cal-ves in our laboratory for detecting BVDV antibody titers and antigen we noticed that successful virus propaga-tion can occur from heat inactivated (in a water bath at 56° C for 30 min) 2 serum samples. For further confirma-tion of virus propagation in heat inactivated serum samples, 11 sera which were previously detected positive for BVDV were also investigated. As a result, from a total of 14 heat inactivated-field originated sera samples virus propagation was confirmed in 4 (28.5%) samples. This result suggests that heat inactivation of the serum is not eliminating the risk of pestivirus contamination, thus interfere the results of virus isolation and serological diag-nostic methods including serum neutralization. Moreover relative resistance of BVDV to heat inactivation may lead to failure in complete inactivation of the virus in fetal calf sera which is an important contaminant for biologicals like cell cultures and attenuated live vaccines.

Kaynakça

  • Alpay, G., Yeşilbağ, K. 2015. Serological relati-onships among subgroups in bovine viral diarr-hea virus genotype 1 (BVDV-1). Vet Microbiol., 175, 1-6.
  • Bolin, S.R., Ridpath, J.F. 1998. Prevalence of bovine viral diarrhea virus genotypes and anti-body against those viral genotypes in fetal bovine serum. J Vet Diagn Invest., 10, 135-139.
  • Cedillo Rosales, S. 2004. Charakterisierung ru-minanter Pestiviren mittels Polymerasekettenre-aktion und monoklonaler Antikörper. Univer-sitätsbibliothek Giessen, Germany, PhD thesis.
  • Giammarioli, M., La Rocca, S,A,, Steinbach, F., Casciari, C., De Mia, G.M. 2011. Genetic and an-tigenic typing of border disease virus (BDV) iso-lates from Italy reveals the existence of a novel BDV group. Vet Microbiol., 147, 231-236.
  • Palomares, R.A., Marley, S.M., Givens, M.D., Gallardo, R.A., Brock, K.V. 2013. Bovine viral diarrhea virus fetal persistent infection after im-munization with a contaminated modified-live vi-rus vaccine. Theriogenology, 79, 1184-1195.
  • Ridpath, J.F. 2003. BVDV genotypes and bioty-pes: practical implications for diagnosis and cont-rol. Biologicals, 31, 127-131.
  • Rossi, C.R., Bridgman, C.R., Kiesel, G.K. 1980. Viral contamination of bovine fetal lung cultures and bovine fetal serum. Am J Vet Res., 41, 1680-1681.
  • Ruibal Brunet, I.J., Noa Romero, E., Rivero Mas, A.T., Martín García, R.Z. 1999. Inactivation of BVDV (experimental model for hepatitis C) using low pH and heat treatment in intravenous human immunoglobulins. Sangre (Barc), 44, 352-356.
  • Sauerbrei, A., Wutzler, P. 2009. Testing thermal resistance of viruses. Arch Virol., 154, 115-119.
  • Vilcek, S., Herring, A., Herring, J., Nettleton, P., Lowings, J., Paton, D. 1994. Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol., 136, 309-323.
  • Zabal, O., Kobrak, A.L., Lager, I.A., Schudel, A.A., Weber, E.L. 2000. Contamination of bovi-ne fetal serum with bovine viral diarrhea virus. Rev Argent Microbiol., 32, 27-32.

Persiste Enfekte Buzağıların İnaktive Edilmiş Serumlarından Bovine Viral Diyare Virusu İzolasyonu

Yıl 2016, Cilt: 35 Sayı: 1-2, 7 - 10, 12.12.2016
https://doi.org/10.30782/uluvfd.393909

Öz

Rutin laboratuvar çalışmaları doğrultusunda BVDV antikor ve antijen taraması istenen üç adet (n:3) buza-ğıya ait serum ve svab örneklerinin incelenmesi sırasında serum örnekleri su banyosunda 56° C’de 30 dakika süresince ısı inaktivasyonu tabi tutulmuş ancak 2 adet serum örneğinde virus çoğalmasının gerçekleştiği görül-müştür. Serumların ısı ile inaktive edilmelerine rağmen hücre kültüründe virus üremesinin gerçekleşmesini araş-tırmak amacıyla daha önceden BVDV pozitif olduğu bilinen 11 adet serum örneği de incelemeye alınmıştır. Sonuç olarak ısı inaktivasyonuna tabi tutulmuş saha menşeli 14 adet serum örneğinin 4 adedinde (%28,5) virus çoğalması tespit edilmiştir. Elde edilen bu veri serum inaktivasyon prosedürünün pestivirus kontaminasyonunu elimine etmede yetersiz kaldığını göstermiştir. Bu durum virus izolasyonu ve serolojik teşhis yöntemlerinin sonuçlarını olumsuz etkileyebilir. Ayrıca BVD virusunun ısı inaktivasyonuna karşı direnci, fötal dana serumlarında bulunabilen virusun tamamen inaktive olmasını engelleyebilmektedir. Buna bağlı olarak BVDV, hücre kültürü ve atenüe canlı aşı gibi biyolojik maddeler için ciddi risk haline gelmektedir.

Kaynakça

  • Alpay, G., Yeşilbağ, K. 2015. Serological relati-onships among subgroups in bovine viral diarr-hea virus genotype 1 (BVDV-1). Vet Microbiol., 175, 1-6.
  • Bolin, S.R., Ridpath, J.F. 1998. Prevalence of bovine viral diarrhea virus genotypes and anti-body against those viral genotypes in fetal bovine serum. J Vet Diagn Invest., 10, 135-139.
  • Cedillo Rosales, S. 2004. Charakterisierung ru-minanter Pestiviren mittels Polymerasekettenre-aktion und monoklonaler Antikörper. Univer-sitätsbibliothek Giessen, Germany, PhD thesis.
  • Giammarioli, M., La Rocca, S,A,, Steinbach, F., Casciari, C., De Mia, G.M. 2011. Genetic and an-tigenic typing of border disease virus (BDV) iso-lates from Italy reveals the existence of a novel BDV group. Vet Microbiol., 147, 231-236.
  • Palomares, R.A., Marley, S.M., Givens, M.D., Gallardo, R.A., Brock, K.V. 2013. Bovine viral diarrhea virus fetal persistent infection after im-munization with a contaminated modified-live vi-rus vaccine. Theriogenology, 79, 1184-1195.
  • Ridpath, J.F. 2003. BVDV genotypes and bioty-pes: practical implications for diagnosis and cont-rol. Biologicals, 31, 127-131.
  • Rossi, C.R., Bridgman, C.R., Kiesel, G.K. 1980. Viral contamination of bovine fetal lung cultures and bovine fetal serum. Am J Vet Res., 41, 1680-1681.
  • Ruibal Brunet, I.J., Noa Romero, E., Rivero Mas, A.T., Martín García, R.Z. 1999. Inactivation of BVDV (experimental model for hepatitis C) using low pH and heat treatment in intravenous human immunoglobulins. Sangre (Barc), 44, 352-356.
  • Sauerbrei, A., Wutzler, P. 2009. Testing thermal resistance of viruses. Arch Virol., 154, 115-119.
  • Vilcek, S., Herring, A., Herring, J., Nettleton, P., Lowings, J., Paton, D. 1994. Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol., 136, 309-323.
  • Zabal, O., Kobrak, A.L., Lager, I.A., Schudel, A.A., Weber, E.L. 2000. Contamination of bovi-ne fetal serum with bovine viral diarrhea virus. Rev Argent Microbiol., 32, 27-32.
Toplam 11 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Pelin Tuncer-göktuna Bu kişi benim

Kadir Yeşılbağ

Yayımlanma Tarihi 12 Aralık 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 35 Sayı: 1-2

Kaynak Göster

APA Tuncer-göktuna, P., & Yeşılbağ, K. (2016). Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 35(1-2), 7-10. https://doi.org/10.30782/uluvfd.393909
AMA Tuncer-göktuna P, Yeşılbağ K. Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. Aralık 2016;35(1-2):7-10. doi:10.30782/uluvfd.393909
Chicago Tuncer-göktuna, Pelin, ve Kadir Yeşılbağ. “Bovine Viral Diarrhea Virus Propagates in Cell Culture After Inoculation of Inactivated Sera from Persistently Infected Calves”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 35, sy. 1-2 (Aralık 2016): 7-10. https://doi.org/10.30782/uluvfd.393909.
EndNote Tuncer-göktuna P, Yeşılbağ K (01 Aralık 2016) Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 35 1-2 7–10.
IEEE P. Tuncer-göktuna ve K. Yeşılbağ, “Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves”, Uludağ Üniversitesi Veteriner Fakültesi Dergisi, c. 35, sy. 1-2, ss. 7–10, 2016, doi: 10.30782/uluvfd.393909.
ISNAD Tuncer-göktuna, Pelin - Yeşılbağ, Kadir. “Bovine Viral Diarrhea Virus Propagates in Cell Culture After Inoculation of Inactivated Sera from Persistently Infected Calves”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 35/1-2 (Aralık 2016), 7-10. https://doi.org/10.30782/uluvfd.393909.
JAMA Tuncer-göktuna P, Yeşılbağ K. Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2016;35:7–10.
MLA Tuncer-göktuna, Pelin ve Kadir Yeşılbağ. “Bovine Viral Diarrhea Virus Propagates in Cell Culture After Inoculation of Inactivated Sera from Persistently Infected Calves”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, c. 35, sy. 1-2, 2016, ss. 7-10, doi:10.30782/uluvfd.393909.
Vancouver Tuncer-göktuna P, Yeşılbağ K. Bovine Viral Diarrhea Virus Propagates in Cell Culture after Inoculation of Inactivated Sera from Persistently Infected Calves. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2016;35(1-2):7-10.