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Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi

Yıl 2020, Cilt: 46 Sayı: 3, 321 - 327, 01.12.2020
https://doi.org/10.32708/uutfd.796517

Öz

Temporomandibular eklem (TME), memeli çenesinin hareketleri için gerekli olan sinoviyal bir eklemdir, vücutta en sık kullanılan eklemlerden biridir. Yaşlı hastalarda primer olarak görülebilen, bazı patolojik durumlarda (travma ve internal derangement) sekonder olarak gelişen osteoartrit TME’de en sık görülen artrit tipidir. TME patolojilerinin, kıkırdak dokunun histolojik yapısı gereği iyileşme potansiyeli düşük olduğu için, klinik tedavileri sınırlıdır. Bu çalışmada farklı hücrelere dönüşme potansiyeli olan yağ doku kaynaklı kök hücrelerin, birçok büyüme faktörü barındıran Trombositten Zengin Fibrin (PRF) ile uyarılarak, hasar oluşturulan TME kıkırdağı üzerindeki kombine etkilerinin araştırılması amaçlandı. Çalışmada tavşanların her iki TME eklem disk laterallerinde 3 mm’lik defektler oluşturuldu. Sağ tarafa ense-sırt bölgesinden alınan yağ grefti ve tavşanın kendi kanından hazırlanan PRF yerleştirildi, sol tarafı sham grubu olarak planlanıp başka işlem yapılmadan kapatıldı. 8 hafta sonunda eklem kondil ve diskleri çıkarıldı, Hematoksilen&Eozin (H&E) ve Safranin O-Fast Green metodları ile boyanarak incelendi. Her iki grup için eklem yüzey yapısı, kondrosit yoğunluğu, hücre kümelenmesi ve Safranin O-Fast Green boyanma kaybı histopatolojik olarak derecelendirildi. Değerlendirilen parametreler arasında anlamlı fark saptanmadığı için, deneysel TME defekt modelinin onarımında yağ grefti ve PRF kombinasyonu etkili bulunmadı.

Teşekkür

Histolojik kesitlerin preparasyonuna katkıları için Biyolog Ayşe AKBAŞ'a teşekkürler.

Kaynakça

  • Cui D, Li H, Xu X, et al. Mesenchymal stem cells for cartilage regeneration of TMJ Osteoarthritis. Stem Cells Int 2017;Article ID 5979741, 11.
  • Embree MC, Iwaoka GM, Kong D, et al. Soft tissue ossification and condylar cartilage degeneration following TMJ disc perforation in a rabbit pilot study. Osteoarthritis Cartilage 2015;23:629-39.
  • Huang H, Shank G ,Ma L, Tallents RH, Kyrkanides S. Nerve growth factor induced after temporomandibularjoint inflammation decelerates chondrocyte differentiation. Oral Dis 2013;19:604-10.
  • Imai H, Sakamoto I, Yoda T, Yamashita Y. A model for internal derangement and osteoarthritis of the temporomandibular joint with experimental traction of the mandibular ramus in rabbit. Oral Dis 2001;7:185-91.
  • Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell based therapies. Tissue Eng 2001;7:211-28.
  • Gimble JM, Katz AJ, Bunnel BA. Adipose derived stem cells for regenerative medicine. Circ Res 2007;100:1249-60.
  • Choukroun J, Adda F, Schoeffler C, Vervelle A. The opportunity in perio-implantology: the PRF [in French]. Implantodontie 2001; 42:55-62.
  • Maruyama M, Satake H, Suzuki T, et al. Comparison of the effects of osteochondral autograft transplantation with platelet rich-plasma or platelet-rich fibrin on osteochondral defects in a rabbit model. Am J Sports Med 2017; 45:3280-8.
  • Kazemi D, Fakhrjou A, Dizaji VM, Alishahi MK. Effect of autologous platelet rich fibrin on the healing of experimental articular cartilage defects of the knee in an animal model. Biomed Res Int 2014;2014:486436.
  • Suzuki T, Bessho K, Fujimura K, et al. Regeneration of defects in the articular cartilage in rabbit temporomandibular joints by bone morphogenetic protein-2. Br J Oral Maxillofac Surg 2002;40:201-6.
  • Takafuji H, Suzuki T, Okubo Y, Fujimura K, Bessho K. Regeneration of articular cartilage defects in the temporomandibular joint of rabbits by fibroblast growth factor-2: a pilot study. Int J Oral and Maxillofacial Surg 2007;36:934-7.
  • Mazzetti MPV, Oliveira IS, Miranda-Ferreira R, et al. Qualitative and quantitative analysis of rabbit’s fat mesenchymal stem cells. Acta Cir Bras 2010;25(1):24-7.
  • Laverty S, Girard CA, Williams JM, Hunziker EB, Pritzker KPH. The OARSI histopathology initiative-recommendations for histological assessments of osteoarthritis in the rabbit. Osteoarthritis Cartilage 2010;18:53-65.
  • Murphy MK, MacBarb RF, Wong ME, Athanasiou KA. Temporomandibular joint disorders: A review of etiology, clinical management, and tissue engineering strategies. Int J Oral Maxilofac Implants 2013;28(6):393-414.
  • Kalay A, Kahveci R, Kahveci Z, Halk KZ. Temporomandibular Eklem Disk Bozukluklarında Yağ Greftinin Etkisi. Uludağ Üniversitesi Tıp Fakültesi Dergisi 2018;44(3):159-66.
  • Woo CH, Kim HK, Jung GY, et al. Small extracellular vesicles from human adipose-derived stem cells attenuate cartilage degeneration. J Extracell Vesicles 2020;9(1): 1735249.
  • Kütük N, Baş B, Soylu E, et al. Effect of platelet-rich plasma on fibrocartilage, cartilage, and bone repair in temporomandibular joint. J Oral Maxillofac Surg 2014;72:277-84.
  • Schär MO, Diaz-Romero J, Kohl S, Zumstein MA, Nesic D. Platelet-rich concentrates differentially release growth factors and induce cell migration in vitro. Clin Orthop Relat Res 2015;473(5):1635-43.
  • Lietman SA, Miyamoto S, Brown PR, Inoue N, Reddi AH. The temporal sequence of spontaneous repair of osteochondral defects in the knees of rabbits is dependent on the geometry of the defect. J Bone Joint Surg Br 2002;84(4):600-6.
  • Xie X, Wang Y, Zhao C, et al. Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. Biomaterials 2012;33:7008-18.

Combined Effect of Fat Graft and Platelet–Rich Fibrin on Tissue Recovery in Temporomandibular Joint Defect

Yıl 2020, Cilt: 46 Sayı: 3, 321 - 327, 01.12.2020
https://doi.org/10.32708/uutfd.796517

Öz

Temporomandibular joint (TMJ), is a synovial joint necessary for the movements of mammal chin and one of the most frequently utilized joints of the body. Osteoarthritis that is formed primarily on elder patients or secondarily on some pathological cases (trauma and internal derangement) is the most common type of arthritis of TMJ. Recovery potential of TMJ pathologies is low due to the histological structure of the cartilage tissue and therefore, clinical treatment of these pathologies is limited. In this study, the effects of fat tissue-derived stem cells that have the ability to differantiate into different cells and is stimulated with Platelet-Rich Fibrin (PRF) that contains many growth factors on artificially damaged TMJ is investigated. In this study, 3 mm defects were created on the lateral sides of both TMJ discs of the rabbits. Fat grafts obtained from the neck-dorsal region and PRF prepared from the rabbits’ own blood were placed on the right side and the left side of each rabbit is planned as the sham group and closed without further treatment. After eight weeks, joint condyles and discs were removed, stained with Haematoxylen&Eosin (H&E) and Safranin O/Fast Green and histologically examined. Joint surface structures, chondrocyte densities, cell aggregations and losses of Safranin O/Fast Green staining of both of the groups were scored histopathologically. There was no significant difference between investigated parameters and thus, fat graft and PRF combination wasn’t concluded to be effective on experimental TMJ defect model recovery.

Kaynakça

  • Cui D, Li H, Xu X, et al. Mesenchymal stem cells for cartilage regeneration of TMJ Osteoarthritis. Stem Cells Int 2017;Article ID 5979741, 11.
  • Embree MC, Iwaoka GM, Kong D, et al. Soft tissue ossification and condylar cartilage degeneration following TMJ disc perforation in a rabbit pilot study. Osteoarthritis Cartilage 2015;23:629-39.
  • Huang H, Shank G ,Ma L, Tallents RH, Kyrkanides S. Nerve growth factor induced after temporomandibularjoint inflammation decelerates chondrocyte differentiation. Oral Dis 2013;19:604-10.
  • Imai H, Sakamoto I, Yoda T, Yamashita Y. A model for internal derangement and osteoarthritis of the temporomandibular joint with experimental traction of the mandibular ramus in rabbit. Oral Dis 2001;7:185-91.
  • Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell based therapies. Tissue Eng 2001;7:211-28.
  • Gimble JM, Katz AJ, Bunnel BA. Adipose derived stem cells for regenerative medicine. Circ Res 2007;100:1249-60.
  • Choukroun J, Adda F, Schoeffler C, Vervelle A. The opportunity in perio-implantology: the PRF [in French]. Implantodontie 2001; 42:55-62.
  • Maruyama M, Satake H, Suzuki T, et al. Comparison of the effects of osteochondral autograft transplantation with platelet rich-plasma or platelet-rich fibrin on osteochondral defects in a rabbit model. Am J Sports Med 2017; 45:3280-8.
  • Kazemi D, Fakhrjou A, Dizaji VM, Alishahi MK. Effect of autologous platelet rich fibrin on the healing of experimental articular cartilage defects of the knee in an animal model. Biomed Res Int 2014;2014:486436.
  • Suzuki T, Bessho K, Fujimura K, et al. Regeneration of defects in the articular cartilage in rabbit temporomandibular joints by bone morphogenetic protein-2. Br J Oral Maxillofac Surg 2002;40:201-6.
  • Takafuji H, Suzuki T, Okubo Y, Fujimura K, Bessho K. Regeneration of articular cartilage defects in the temporomandibular joint of rabbits by fibroblast growth factor-2: a pilot study. Int J Oral and Maxillofacial Surg 2007;36:934-7.
  • Mazzetti MPV, Oliveira IS, Miranda-Ferreira R, et al. Qualitative and quantitative analysis of rabbit’s fat mesenchymal stem cells. Acta Cir Bras 2010;25(1):24-7.
  • Laverty S, Girard CA, Williams JM, Hunziker EB, Pritzker KPH. The OARSI histopathology initiative-recommendations for histological assessments of osteoarthritis in the rabbit. Osteoarthritis Cartilage 2010;18:53-65.
  • Murphy MK, MacBarb RF, Wong ME, Athanasiou KA. Temporomandibular joint disorders: A review of etiology, clinical management, and tissue engineering strategies. Int J Oral Maxilofac Implants 2013;28(6):393-414.
  • Kalay A, Kahveci R, Kahveci Z, Halk KZ. Temporomandibular Eklem Disk Bozukluklarında Yağ Greftinin Etkisi. Uludağ Üniversitesi Tıp Fakültesi Dergisi 2018;44(3):159-66.
  • Woo CH, Kim HK, Jung GY, et al. Small extracellular vesicles from human adipose-derived stem cells attenuate cartilage degeneration. J Extracell Vesicles 2020;9(1): 1735249.
  • Kütük N, Baş B, Soylu E, et al. Effect of platelet-rich plasma on fibrocartilage, cartilage, and bone repair in temporomandibular joint. J Oral Maxillofac Surg 2014;72:277-84.
  • Schär MO, Diaz-Romero J, Kohl S, Zumstein MA, Nesic D. Platelet-rich concentrates differentially release growth factors and induce cell migration in vitro. Clin Orthop Relat Res 2015;473(5):1635-43.
  • Lietman SA, Miyamoto S, Brown PR, Inoue N, Reddi AH. The temporal sequence of spontaneous repair of osteochondral defects in the knees of rabbits is dependent on the geometry of the defect. J Bone Joint Surg Br 2002;84(4):600-6.
  • Xie X, Wang Y, Zhao C, et al. Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration. Biomaterials 2012;33:7008-18.
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Klinik Tıp Bilimleri (Diğer)
Bölüm Özgün Araştırma Makaleleri
Yazarlar

Mehmet Köse Bu kişi benim 0000-0003-2148-2355

Senem Esin Yavaş 0000-0002-6949-1210

Semiha Ersoy 0000-0002-6419-0304

Ramazan Kahveci 0000-0002-3746-6280

Yayımlanma Tarihi 1 Aralık 2020
Kabul Tarihi 3 Kasım 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 46 Sayı: 3

Kaynak Göster

APA Köse, M., Yavaş, S. E., Ersoy, S., Kahveci, R. (2020). Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 46(3), 321-327. https://doi.org/10.32708/uutfd.796517
AMA Köse M, Yavaş SE, Ersoy S, Kahveci R. Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi. Uludağ Tıp Derg. Aralık 2020;46(3):321-327. doi:10.32708/uutfd.796517
Chicago Köse, Mehmet, Senem Esin Yavaş, Semiha Ersoy, ve Ramazan Kahveci. “Temporomandibular Eklem Bozukluklarında Yağ Grefti Ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi”. Uludağ Üniversitesi Tıp Fakültesi Dergisi 46, sy. 3 (Aralık 2020): 321-27. https://doi.org/10.32708/uutfd.796517.
EndNote Köse M, Yavaş SE, Ersoy S, Kahveci R (01 Aralık 2020) Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi. Uludağ Üniversitesi Tıp Fakültesi Dergisi 46 3 321–327.
IEEE M. Köse, S. E. Yavaş, S. Ersoy, ve R. Kahveci, “Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi”, Uludağ Tıp Derg, c. 46, sy. 3, ss. 321–327, 2020, doi: 10.32708/uutfd.796517.
ISNAD Köse, Mehmet vd. “Temporomandibular Eklem Bozukluklarında Yağ Grefti Ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi”. Uludağ Üniversitesi Tıp Fakültesi Dergisi 46/3 (Aralık 2020), 321-327. https://doi.org/10.32708/uutfd.796517.
JAMA Köse M, Yavaş SE, Ersoy S, Kahveci R. Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi. Uludağ Tıp Derg. 2020;46:321–327.
MLA Köse, Mehmet vd. “Temporomandibular Eklem Bozukluklarında Yağ Grefti Ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi”. Uludağ Üniversitesi Tıp Fakültesi Dergisi, c. 46, sy. 3, 2020, ss. 321-7, doi:10.32708/uutfd.796517.
Vancouver Köse M, Yavaş SE, Ersoy S, Kahveci R. Temporomandibular Eklem Bozukluklarında Yağ Grefti ve Trombositten Zengin Fibrinin Doku Onarımı Üzerine Kombine Etkisi. Uludağ Tıp Derg. 2020;46(3):321-7.

ISSN: 1300-414X, e-ISSN: 2645-9027

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