Existence of Plasmidic AmpC Beta-Lactamase-Producing Escherichia coli Isolates in Healthy Laying Hens
Öz
In the
present study, determination of prevalence
of plasmidic AmpC (pAmpC) beta-lactamase-producing Escherichia coli in intestinal flora of laying hens in Burdur
province of Turkey and characterization of pAmpC beta-lactamase-producing E. coli isolates were aimed. Two
hundreds twenty five fecal samples from all laying hen farms (n=4) in Burdur
province were collected and cultured in Brilliance E. coli/coliform selective agar supplemented with cefotaxime or
ceftazidime. Presumptive AmpC beta-lactamase-producing E. coli isolates were determined by a phenotypic test and the
isolates were screened by PCR for blapAmpC
genes. Susceptibilities of the E. coli
isolates to beta-lactams and other classes of antibiotics were investigated by
agar disc diffusion test and finally phylogenetic analysis of the E. coli isolates was performed by
multiplex PCR. pAmpC beta-lactamase-producing E. coli was isolated from 15 (6.7%) laying hen fecal samples. blaCITM family gene was found
in all E. coli isolates. The pAmpC
beta-lactamase-producing isolates showed co-resistance to several classes of
antibiotics (aminoglycosides, quinolones, sulfamethoxazole-trimethoprim and
tetracycline). According to phylogenetic analysis, the E. coli isolates belonged to A1, B1 and D1
groups. Consequently, by the present study the first isolation of pAmpC
beta-lactamase-producing E. coli
isolates with multidrug-resistance phenotype on lying hen production from
Turkey was reported.
Anahtar Kelimeler
Chicken Escherichia coli Multiple antibacterial drug resistance AmpC beta-lactamases
Kaynakça
- Basaran Kahraman B, Diren Sigirci B, Celik B, et al. (2016). Detection of extended-spectrum beta-lactamase and AmpC beta-lactamase producing Escherichia coli isolates from chickens. Kafkas Univ Vet Fak Derg, 22(4), 591-596. Campos TA, Lago JC, Nakazato G, et al. (2008). Occurrence of virulence-related sequences and phylogenetic analysis of commensal and pathogenic avian Escherichia coli strains (APEC). Pesq Vet Bras, 28 (10), 533-540. Clermont O, Bonacorsi S, Bingen E (2000). Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol, 66, 4555-4558. CLSI (Clinical and Laboratory Standards Institute) (2010). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, Approved standard. 3rd Ed. CLSI document M31-A3, Wayne, Pennsylvania.
- CLSI (Clinical and Laboratory Standards Institute) (2013). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Second informational supplement, CLSI document VET01-S2, Wayne, Pennsylvania. CLSI (Clinical and Laboratory Standards Institute) (2014). Performance standards for antimicrobial susceptibility testing. Twenty-fourth informational supplement, CLSI document M100-S24, Wayne, Pennsylvania. Escobar-Páramo P, Le Menac'h A, Le Gall T, et al. (2006). Identification of forces shaping the commensal Escherichia coli genetic structure by comparing animal and human isolates. Environ Microbiol, 8, 1975-1984. Frere JM (1995). Beta-lactamases and bacterial resistance to antibiotics. Mol Microbiol, 16, 385-395. Higgins J, Hohn C, Hornor S, Frana M, Denver M, Joerger R (2007). Genotyping of Escherichia coli from environmental and animal samples. J Microbiol Methods, 70, 227-235. Hille K, Fischer J, Falgenhauer L, et al. (2014). On the occurence of extended-spectrum- and AmpC-beta-lactamase-producing Escherichia coli in livestock: results of selected European studies. Berl Munch Tierarztl Wochenschr, 127 (9-10), 403-411. Jacoby GA (2009). AmpC β-Lactamases. Clin Microbiol Rev, 22, 161-82. Kameyama M, Chuma T, Yabata J, Tominaga K, Iwata H, Okamoto K (2013). Prevalence and epidemiological relationship of CMY-2 AmpC β-lactamase and CTX-M extended-spectrum β-lactamase-producing Escherichia coli isolates from broiler farms in Japan. J Vet Med Sci, 75(8), 1009-1915. Laube H, Friese A, von Salviati C, et al. (2013). Longitudinal monitoring of extended-spectrum-beta-lactamase/AmpC-producing Escherichia coli at German broiler chicken fattening farms. Appl Environ Microbiol, 79 (16), 4815-4820. Maamar E, Hammami S, Alonso CA, et al. (2016). High prevalence of extended-spectrum and plasmidic AmpC beta-lactamase-producing Escherichia coli from poultry in Tunisia. Int J Food Microbiol, 231, 69-75. Pérez-Pérez FJ, Hanson ND (2002). Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol, 40, 2153-2162. Thomson KS (2001). Controversies about extended-spectrum and AmpC beta-lactamases. Emerg Infect Dis, 7 (2), 333-336. Unal N, Dilik Z, Askar S, Bedir O (2014). Prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli isolates in fecal samples of broilers. 11th National Congress of Veterinary Microbiology, October 21-24, Antalya (Turkey), 2014. Voets GM, Fluit AC, Scharringa J, et al. (2013). Identical plasmid AmpC beta-lactamase genes and plasmid types in E. coli isolates from patients and poultry meat in the Netherlands. Int J Food Microbiol, 167, 359-362. Wang G, Clark CG, Rodgers FG (2002). Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 shiga toxin family by multiplex PCR. J Clin Microbiol, 40, 3613-3619. Wasyl D, Hasman H, Cavaco LM, Aarestrup FM (2012). Prevalence and characterization of cephalosporin resistance in nonpathogenic Escherichia coli from food-producing animals slaughtered in Poland. Microb Drug Resist, 18, 79-82. Winn W, Allen S, Janda W, et al. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. Lippincott Williams and Wilkins, Philadelphia.
Öz
u
çalışmada, Burdur ilinde yetiştirilen yumurtacı tavukların bağırsak
mikroflorasında plazmid kökenli AmpC (pAmpC) beta laktamaz üreten Escherichia coli izolatlarının
prevalansını belirlemek ve belirlenen pAmpC beta laktamaz üreten E. coli izolatlarının
karakterizasyonunun yapılması amaçlandı.
Burdur ilindeki tüm yumurtacı tavuk çiftliklerinden (n=4) 225 dışkı örneği
toplandı ve içerisine sefotaksim veya seftazidim ilave edilerek hazırlanmış E. coli/koliform selektif agarda dışkı
örneklerinin kültürleri yapıldı. AmpC beta laktamaz üreten E. coli izolatlarının ön
tanısı fenotipik bir test ile yapıldı ve izolatlar blapAmpC beta laktamaz genleri için PZR ile tarandı. E. coli izolatlarının beta laktam ve
diğer sınıflardan antibiyotiklere olan duyarlılıkları agar disk difüzyon testi
ile araştırıldı ve son olarak E. coli
izolatlarının filogenetik analizi multipleks PZR ile gerçekleştirildi. pAmpC
beta laktamaz üreten E. coli 15 adet
(%6.7) dışkı örneğinden izole edildi. CIT familyasına ait blapAmpC geni (blaCIT)
tüm E. coli izolatlarında bulundu.
pAmpC beta laktamaz üreten izolatlar çeşitli sınıflardan antibiyotiklere
(aminoglikozidler, kinolonlar, sulfametoksazol-trimethoprim ve tetrasiklinler)
karşı dirençli bulundu. Filogenetik analiz sonuçlarına göre E. coli izolatları A1, B1 ve
D1 gruplarına ait oldukları belirlendi. Sonuç olarak, Türkiye’de
yumurtacı tavuk üretiminde pAmpC beta laktamaz üreten ve çoklu antibiyotik
direnci gösteren E. coli
izolatlarının ilk kez bu çalışmayla ortaya konulduğu görüldü.
Anahtar Kelimeler
Tavuk Escherichia coli Çoklu antibakteryel ilaç dirençliliği AmpC beta laktamazlar
Kaynakça
- Basaran Kahraman B, Diren Sigirci B, Celik B, et al. (2016). Detection of extended-spectrum beta-lactamase and AmpC beta-lactamase producing Escherichia coli isolates from chickens. Kafkas Univ Vet Fak Derg, 22(4), 591-596. Campos TA, Lago JC, Nakazato G, et al. (2008). Occurrence of virulence-related sequences and phylogenetic analysis of commensal and pathogenic avian Escherichia coli strains (APEC). Pesq Vet Bras, 28 (10), 533-540. Clermont O, Bonacorsi S, Bingen E (2000). Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol, 66, 4555-4558. CLSI (Clinical and Laboratory Standards Institute) (2010). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, Approved standard. 3rd Ed. CLSI document M31-A3, Wayne, Pennsylvania.
- CLSI (Clinical and Laboratory Standards Institute) (2013). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Second informational supplement, CLSI document VET01-S2, Wayne, Pennsylvania. CLSI (Clinical and Laboratory Standards Institute) (2014). Performance standards for antimicrobial susceptibility testing. Twenty-fourth informational supplement, CLSI document M100-S24, Wayne, Pennsylvania. Escobar-Páramo P, Le Menac'h A, Le Gall T, et al. (2006). Identification of forces shaping the commensal Escherichia coli genetic structure by comparing animal and human isolates. Environ Microbiol, 8, 1975-1984. Frere JM (1995). Beta-lactamases and bacterial resistance to antibiotics. Mol Microbiol, 16, 385-395. Higgins J, Hohn C, Hornor S, Frana M, Denver M, Joerger R (2007). Genotyping of Escherichia coli from environmental and animal samples. J Microbiol Methods, 70, 227-235. Hille K, Fischer J, Falgenhauer L, et al. (2014). On the occurence of extended-spectrum- and AmpC-beta-lactamase-producing Escherichia coli in livestock: results of selected European studies. Berl Munch Tierarztl Wochenschr, 127 (9-10), 403-411. Jacoby GA (2009). AmpC β-Lactamases. Clin Microbiol Rev, 22, 161-82. Kameyama M, Chuma T, Yabata J, Tominaga K, Iwata H, Okamoto K (2013). Prevalence and epidemiological relationship of CMY-2 AmpC β-lactamase and CTX-M extended-spectrum β-lactamase-producing Escherichia coli isolates from broiler farms in Japan. J Vet Med Sci, 75(8), 1009-1915. Laube H, Friese A, von Salviati C, et al. (2013). Longitudinal monitoring of extended-spectrum-beta-lactamase/AmpC-producing Escherichia coli at German broiler chicken fattening farms. Appl Environ Microbiol, 79 (16), 4815-4820. Maamar E, Hammami S, Alonso CA, et al. (2016). High prevalence of extended-spectrum and plasmidic AmpC beta-lactamase-producing Escherichia coli from poultry in Tunisia. Int J Food Microbiol, 231, 69-75. Pérez-Pérez FJ, Hanson ND (2002). Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol, 40, 2153-2162. Thomson KS (2001). Controversies about extended-spectrum and AmpC beta-lactamases. Emerg Infect Dis, 7 (2), 333-336. Unal N, Dilik Z, Askar S, Bedir O (2014). Prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli isolates in fecal samples of broilers. 11th National Congress of Veterinary Microbiology, October 21-24, Antalya (Turkey), 2014. Voets GM, Fluit AC, Scharringa J, et al. (2013). Identical plasmid AmpC beta-lactamase genes and plasmid types in E. coli isolates from patients and poultry meat in the Netherlands. Int J Food Microbiol, 167, 359-362. Wang G, Clark CG, Rodgers FG (2002). Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 shiga toxin family by multiplex PCR. J Clin Microbiol, 40, 3613-3619. Wasyl D, Hasman H, Cavaco LM, Aarestrup FM (2012). Prevalence and characterization of cephalosporin resistance in nonpathogenic Escherichia coli from food-producing animals slaughtered in Poland. Microb Drug Resist, 18, 79-82. Winn W, Allen S, Janda W, et al. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. Lippincott Williams and Wilkins, Philadelphia.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 20 Ağustos 2017 |
| Gönderilme Tarihi | 19 Ekim 2016 |
| Kabul Tarihi | 26 Ocak 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 28 Sayı: 2 |
Kaynak Göster
Kabul edilen makaleler Creative Commons Atıf-Ticari Olmayan Lisansla Paylaş 4.0 uluslararası lisansı ile lisanslanmıştır.