Araştırma Makalesi
BibTex RIS Kaynak Göster

Investigation of Virulence Genes in Escherichia coli O157:H7 Strains Isolated from Dairy Cattle

Yıl 2016, Cilt: 27 Sayı: 3, 161 - 164, 20.12.2016

Öz

Escherichia coli O157:H7 is one of the most important zoonotic agents worldwide. Humans can be infected by consumption of food and water contaminated with agent. Cattle areespecially considered to be the main reservoir of E. coli O157:H7.The aims of this study were to investigate the presence of E. coli O157:H7 in dairy cattle in Elazığ, and to determine virulence genes in E. coli O157:H7 isolates. A total of 300 rectal swab samples were collected from dairy cattle.E. coli O157:H7 was isolated from 11 (3.6 %) of rectal swab samples.The presence of shiga toxin (stx1 and stx2), intimin (eae) and haemolysin (hlyA) geneswas detected by polymerase chain reaction (PCR). Of the 11E. coli O157:H7 strains, 9 had stx2, hlyAand eae genes. Two strains were positive for stx1, stx2, hlyA and eae genes. The results of this study show that dairy cattle are asymptomatic carriers of E. coli O157:H7 that is pathogenic for humans.

Kaynakça

  • Akkaya L, Alişarlı M, Kara R, Telli R (2007). Afyonkarahisar’da tüketime sunulan çig süt ve peynirlerde E. coli O157:H7 varlıgının belirlenmesi. YYÜ Vet Fak Derg, 18, 1-5. Amezquita-Lopez BA, Quinones B, Cooley MB, Leon-Felix J, Campo NC, Mandrel RE, Jimenez M, Chaidez C (2012). Genotypic analyses of shiga toxin-producing Escherichia coli O157 and non-O157 recovered from feces of domestic animals on rural farms in Mexico. PLoS One, 7, 1-12. AslantasÖ, Erdogan S, Cantekin Z, GulactıI, Evrendilek GA (2006). Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from Turkish cattle. Int J Food Microbiol, 106, 338-342. Aspan A, Eriksson E (2010). Verotoxigenic Escherichia coli O157:H7 from Swedish cattle; isolates from prevalence studies versus strains linked to human infections–a retrospective study. BMC Vet Res, 6, 1-10. Aydın F, İça T, Yontar A (2010). Kayseri yöresinde süt sığırlarında Escherichia coli O157:H7’nin konvansiyonel ve moleküler yöntemlerle araştırılması. Erciyes Üniv Sağ Bil Derg, 19, 159-166. Bidet P, Mariani-Kurkdjian P, Grimont F, Brahimi N, Courroux C, Grimont P, Bingen E (2005). Characterization of Escherichia coli O157:H7 isolates causing haemolytic uraemic syndrome in France. J Med Microbiol, 54, 71-75. Chui L, Li V, Fach P, Delannoy S, Malejczyk K, Patterson-Fortin L, Poon A, King R, Simmonds K, Scott AN, Lee MC (2015). Molecular profiling of Escherichia coli O157:H7 and non-O157 strains isolated from humans and cattle in Alberta, Canada .J Clin Microbiol, 53, 986-990. Çabalar M, Boynukara B, Gülhan T, Ekin IH (2001). Van yöresinde sağlıklı görülen süt sığırcılığı işletmelerinde Rotavirus, E. coli K99 and O157:H7’nin varlığı üzerine araştırmalar. Turk J Vet Anim Sci, 25, 191- 196. D’Astek BA, del Castillo LL, Miliwebsky E, Carbonari C, Palladino PM, Deza N, Chinen I, Manfredi E, Leotta GA, Masana MO, Rivas M (2012). Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina. Foodborne Pathog Dis, 9, 457-464. Erdoğan, H., Erdoğan, A., Levent, B., Kayalı, R., Arslan, H., 2008. Enterohemorrhagic Escherichia coli O157:H7: case report. Turk J Pediatr, 50, 488-491. Erdoğan H, Levent B, Erdoğan A, Güleşen R, Arslan H (2011). Investigation of verotoxigenic Escherichia coli O157:H7 incidence in gastroenteritis patients. Bull Microbiol, 45, 519-525 Ferreira MRA, Filho EGF, Pinto JFN, Dias M, Moreira CN (2014). Isolation, prevalence, and risk factors for infection by shiga toxinproducing Escherichia coli (STEC) in dairy cattle. Trop Anim Health Prod, 46, 635-639. Fratamico PM, Debroy C, Liu Y (2009). The DNA sequence of the Escherichia coli O22 O-antigen gene cluster and detection of pathogenic strains belonging to E. coli serogroups O22 and O91 by multiplex PCR assays targeting virulence genes and genes in the respective O-antigen gene clusters. Food Anal Method, 2, 169-179. Gannon VPJ, Rashed M, King RK, ThomasEG (1993). Detection and characterization of the eae gene of shiga-like toxin producing Escherichia coli using polymerase chain reaction. J Clin Microbiol, 31, 1268-1274. HiroiM, Takahashi N, Harada T, Kawamori F, IadaN, Kanda T, Sugiyama K, Ohashi N, Hara-Kudo Y, Masuda, T (2012). Serotype, shiga toxin (stx) type, and antimicrobial resistance of stx-producing Escherichia coli isolated from humans in Shizuoka Prefecture, Japan (2003-2007). Jpn J Infect Dis, 65, 198-202. Hussein HS, Bollinger LM (2005). Prevalence of shiga toxin-producing Escherichia coli in beef cattle. J Food Protect, 68, 2224-2241. Hussein HS, Sakuma T (2005). Prevalence of shiga toxin-producing Escherichiacoli in dairy cattle and their products. J Dairy Sci, 88, 450- 465,2005. Inat, G, Siriken B (2010). Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey. J Vet Sci, 11, 321-326. Islam MA, Mondol AS, de Boer E, Beumer RR, Zwietering MH, Talukder KA, Heuvelink AE (2008). Prevalence and genetic characterization of shiga toxin-producing Escherichia coli isolates from slaughtered animals in Bangladesh. Appl Environ Microbiol, 74, 5414-5421. Kawano K, Ono H, Iwashita O, Kurogi M, Haga T, Maeda K, Goto Y (2012). Stx genotype and molecular epidemiological analyses of Shiga toxin-producing Escherichia coli O157:H7/H-in human and cattle isolates. Eur J Clin Microbiol Infect Dis, 31, 119-127. Keen JE, Wittum TE, Dunn JR, Bono JL, Durso LM (2006). Shiga-toxigenic Escherichia coli O157 in agricultural fair livestock, United States. Emerg Infect Dis, 12, 780-786. KuyucuogluY, Seker E, Sareyyupoglu B, Gurler Z (2011). Virulence genes of shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle. Ankara Univ Vet Fak Derg, 58, 255-260. Money P, Kelly AF, Gould SWJ, Denholm-Price J, Threfall EJ, Fielder MD (2010). Cattle, weather and water: mapping Escherichia coli O157:H7 infections in humans in England and Scotland. Environ Microbiol, 12, 2633-2644. Mora A, Blanco M, Blanco JE, Alanso MP, Dhabi G, Thomson-Carter F, Usera MA, Bartolome R, Prats G, Blanco J (2004). Phage types and genotypes of shiga toxin-producing Escherichia coli O157:H7 isolates from humans and animals in Spain: identification and characterization of two predominating phage types (PT2 and PT8). J Clin Microbiol, 42, 4007-4015. Nagano I, Kunishima M, Itoh Y (1998). Detection of verotoxin producing Escherichia coli O157:H7 by multiplex polymerase chain reaction. Microbiol Immunol, 42, 371-376. Oporto B, Esteban I, Aduriz G, Juste RA, Hurtado A (2008). Escherichia coli O157:H7 and non-O157 shiga toxin-producing E. coli in healthy cattle, sheep, and swine herds in Northern Spain. Zoonose Public Health, 55, 73-81. Öngor H, Kalin R, Çetinkaya B (2007). Investigations of Escherichia coli O157 and some virulence genes in samples of meat and faeces clinically healthy cattle in Turkey. Vet Rec, 161, 392-394. Paton AW, Paton JC (1998). Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eae, enterohemorrhagic E. coli hlyA, rfb O111 and rfb O157.J Clin Microbiol, 36, 598-602. Possé B, De Zutter L, Heyndrickx M, Herman, L (2007). Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli. Res Microbiol, 158, 591-599. Sanchez S, Martinez R, Rey J, Garcia A, Blanco J, Blanco M, Blanco JE, Mora A, Herrera-Leon S, Echeita A, Alonso JM (2010). Phenogenotypic characterisation of Escherichia coli O157:H7 isolates from domestic and wild ruminants. Vet Microbiol, 142, 445-449. Sarımehmetoğlu B, Aksoy M, Ayaz ND, Ayaz Y, Kuplulu O, Kaplan YZ (2009). Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR. Food Control, 20, 357-361. Sasaki Y, Tsujiyama Y, Kusukawa M, Murakami M, Katayama S, Yamada Y (2011). Prevalence and characterization of shiga toxinproducing Escherichia coli O157 and O26 in beef farms. Vet Microbiol,150, 140-145. Scott L, McGee P, Minihan D, Sheridan JJ, Earley B, Leonard N (2006). The cahracterisation of Escherichia coli O157:H7 isolates from cattle faeces and feedlot environment using PFGE. Vet Microbiol, 114, 331-336. Shin SW, Byun JW, Jung M, Shin MK, Yoo HS (2014). Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms. J Microbiol, 52, 285-293. Yılmaz A, Gün H, Yılmaz H (2002). Frequency of Escherichia coli O157:H7 in Turkish cattle. J Food Protect, 65, 1637-1640. Zhou Z, Nishikawa Y, Zhu P, Hong S, Hase A, Cheasty T, Smith HR, Zheng M, Haruki K (2002). Isolation and characterization of shiga toxinproducing Escherichia coli O157:H7 from beef, pork and cattle fecal samples in Changchun, China. J Vet Med Sci, 64, 1041-1044.

Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması

Yıl 2016, Cilt: 27 Sayı: 3, 161 - 164, 20.12.2016

Öz

Escherichia coli O157:H7 tüm dünyada önemli zoonotik etkenlerden biridir. Bu etken ile kontamine olmuş hayvansal gıdalar ve sular insanlar için enfeksiyon kaynağını oluşturmaktadır. Hayvan türleri içerisinde özellikle sığırlar E. coli O157:H7’nin esas taşıyıcısıdır. Bu çalışma, Elazığ’da sütçü sığırlardan E. coli O157:H7’nin izolasyonu ve izolatlarda önemli virulens genlerin varlığının araştırılması amacıyla yapılmıştır. Çalışmada, toplam 300 sütçü sığırdan rektal sıvap örneği alınmıştır. Bu örneklerin 11(% 3.6)’inden E. coli O157:H7 izole edilmiştir. İzole edilen suşlarda Şiga toksin (stx1 ve stx2), intimin (eae) ve enterohemolizin (hlyA) genlerinin varlığı Polimeraz Zincir Reaksiyonu (PZR) ile araştırılmıştır. Suşların 9’unda stx2, hlyA ve eae genleri, 2’sinde isestx1, stx2, hlyA ve eae genleri saptanmıştır. Bu çalışmanın sonuçları sütçü sığırların, insanlar için patojenik karakter taşıyan E. coli O157:H7’nin taşıyıcısı olduğunu göstermektedir.

Kaynakça

  • Akkaya L, Alişarlı M, Kara R, Telli R (2007). Afyonkarahisar’da tüketime sunulan çig süt ve peynirlerde E. coli O157:H7 varlıgının belirlenmesi. YYÜ Vet Fak Derg, 18, 1-5. Amezquita-Lopez BA, Quinones B, Cooley MB, Leon-Felix J, Campo NC, Mandrel RE, Jimenez M, Chaidez C (2012). Genotypic analyses of shiga toxin-producing Escherichia coli O157 and non-O157 recovered from feces of domestic animals on rural farms in Mexico. PLoS One, 7, 1-12. AslantasÖ, Erdogan S, Cantekin Z, GulactıI, Evrendilek GA (2006). Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from Turkish cattle. Int J Food Microbiol, 106, 338-342. Aspan A, Eriksson E (2010). Verotoxigenic Escherichia coli O157:H7 from Swedish cattle; isolates from prevalence studies versus strains linked to human infections–a retrospective study. BMC Vet Res, 6, 1-10. Aydın F, İça T, Yontar A (2010). Kayseri yöresinde süt sığırlarında Escherichia coli O157:H7’nin konvansiyonel ve moleküler yöntemlerle araştırılması. Erciyes Üniv Sağ Bil Derg, 19, 159-166. Bidet P, Mariani-Kurkdjian P, Grimont F, Brahimi N, Courroux C, Grimont P, Bingen E (2005). Characterization of Escherichia coli O157:H7 isolates causing haemolytic uraemic syndrome in France. J Med Microbiol, 54, 71-75. Chui L, Li V, Fach P, Delannoy S, Malejczyk K, Patterson-Fortin L, Poon A, King R, Simmonds K, Scott AN, Lee MC (2015). Molecular profiling of Escherichia coli O157:H7 and non-O157 strains isolated from humans and cattle in Alberta, Canada .J Clin Microbiol, 53, 986-990. Çabalar M, Boynukara B, Gülhan T, Ekin IH (2001). Van yöresinde sağlıklı görülen süt sığırcılığı işletmelerinde Rotavirus, E. coli K99 and O157:H7’nin varlığı üzerine araştırmalar. Turk J Vet Anim Sci, 25, 191- 196. D’Astek BA, del Castillo LL, Miliwebsky E, Carbonari C, Palladino PM, Deza N, Chinen I, Manfredi E, Leotta GA, Masana MO, Rivas M (2012). Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina. Foodborne Pathog Dis, 9, 457-464. Erdoğan, H., Erdoğan, A., Levent, B., Kayalı, R., Arslan, H., 2008. Enterohemorrhagic Escherichia coli O157:H7: case report. Turk J Pediatr, 50, 488-491. Erdoğan H, Levent B, Erdoğan A, Güleşen R, Arslan H (2011). Investigation of verotoxigenic Escherichia coli O157:H7 incidence in gastroenteritis patients. Bull Microbiol, 45, 519-525 Ferreira MRA, Filho EGF, Pinto JFN, Dias M, Moreira CN (2014). Isolation, prevalence, and risk factors for infection by shiga toxinproducing Escherichia coli (STEC) in dairy cattle. Trop Anim Health Prod, 46, 635-639. Fratamico PM, Debroy C, Liu Y (2009). The DNA sequence of the Escherichia coli O22 O-antigen gene cluster and detection of pathogenic strains belonging to E. coli serogroups O22 and O91 by multiplex PCR assays targeting virulence genes and genes in the respective O-antigen gene clusters. Food Anal Method, 2, 169-179. Gannon VPJ, Rashed M, King RK, ThomasEG (1993). Detection and characterization of the eae gene of shiga-like toxin producing Escherichia coli using polymerase chain reaction. J Clin Microbiol, 31, 1268-1274. HiroiM, Takahashi N, Harada T, Kawamori F, IadaN, Kanda T, Sugiyama K, Ohashi N, Hara-Kudo Y, Masuda, T (2012). Serotype, shiga toxin (stx) type, and antimicrobial resistance of stx-producing Escherichia coli isolated from humans in Shizuoka Prefecture, Japan (2003-2007). Jpn J Infect Dis, 65, 198-202. Hussein HS, Bollinger LM (2005). Prevalence of shiga toxin-producing Escherichia coli in beef cattle. J Food Protect, 68, 2224-2241. Hussein HS, Sakuma T (2005). Prevalence of shiga toxin-producing Escherichiacoli in dairy cattle and their products. J Dairy Sci, 88, 450- 465,2005. Inat, G, Siriken B (2010). Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey. J Vet Sci, 11, 321-326. Islam MA, Mondol AS, de Boer E, Beumer RR, Zwietering MH, Talukder KA, Heuvelink AE (2008). Prevalence and genetic characterization of shiga toxin-producing Escherichia coli isolates from slaughtered animals in Bangladesh. Appl Environ Microbiol, 74, 5414-5421. Kawano K, Ono H, Iwashita O, Kurogi M, Haga T, Maeda K, Goto Y (2012). Stx genotype and molecular epidemiological analyses of Shiga toxin-producing Escherichia coli O157:H7/H-in human and cattle isolates. Eur J Clin Microbiol Infect Dis, 31, 119-127. Keen JE, Wittum TE, Dunn JR, Bono JL, Durso LM (2006). Shiga-toxigenic Escherichia coli O157 in agricultural fair livestock, United States. Emerg Infect Dis, 12, 780-786. KuyucuogluY, Seker E, Sareyyupoglu B, Gurler Z (2011). Virulence genes of shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle. Ankara Univ Vet Fak Derg, 58, 255-260. Money P, Kelly AF, Gould SWJ, Denholm-Price J, Threfall EJ, Fielder MD (2010). Cattle, weather and water: mapping Escherichia coli O157:H7 infections in humans in England and Scotland. Environ Microbiol, 12, 2633-2644. Mora A, Blanco M, Blanco JE, Alanso MP, Dhabi G, Thomson-Carter F, Usera MA, Bartolome R, Prats G, Blanco J (2004). Phage types and genotypes of shiga toxin-producing Escherichia coli O157:H7 isolates from humans and animals in Spain: identification and characterization of two predominating phage types (PT2 and PT8). J Clin Microbiol, 42, 4007-4015. Nagano I, Kunishima M, Itoh Y (1998). Detection of verotoxin producing Escherichia coli O157:H7 by multiplex polymerase chain reaction. Microbiol Immunol, 42, 371-376. Oporto B, Esteban I, Aduriz G, Juste RA, Hurtado A (2008). Escherichia coli O157:H7 and non-O157 shiga toxin-producing E. coli in healthy cattle, sheep, and swine herds in Northern Spain. Zoonose Public Health, 55, 73-81. Öngor H, Kalin R, Çetinkaya B (2007). Investigations of Escherichia coli O157 and some virulence genes in samples of meat and faeces clinically healthy cattle in Turkey. Vet Rec, 161, 392-394. Paton AW, Paton JC (1998). Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eae, enterohemorrhagic E. coli hlyA, rfb O111 and rfb O157.J Clin Microbiol, 36, 598-602. Possé B, De Zutter L, Heyndrickx M, Herman, L (2007). Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli. Res Microbiol, 158, 591-599. Sanchez S, Martinez R, Rey J, Garcia A, Blanco J, Blanco M, Blanco JE, Mora A, Herrera-Leon S, Echeita A, Alonso JM (2010). Phenogenotypic characterisation of Escherichia coli O157:H7 isolates from domestic and wild ruminants. Vet Microbiol, 142, 445-449. Sarımehmetoğlu B, Aksoy M, Ayaz ND, Ayaz Y, Kuplulu O, Kaplan YZ (2009). Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR. Food Control, 20, 357-361. Sasaki Y, Tsujiyama Y, Kusukawa M, Murakami M, Katayama S, Yamada Y (2011). Prevalence and characterization of shiga toxinproducing Escherichia coli O157 and O26 in beef farms. Vet Microbiol,150, 140-145. Scott L, McGee P, Minihan D, Sheridan JJ, Earley B, Leonard N (2006). The cahracterisation of Escherichia coli O157:H7 isolates from cattle faeces and feedlot environment using PFGE. Vet Microbiol, 114, 331-336. Shin SW, Byun JW, Jung M, Shin MK, Yoo HS (2014). Antimicrobial resistance, virulence genes and PFGE-profiling of Escherichia coli isolates from South Korean cattle farms. J Microbiol, 52, 285-293. Yılmaz A, Gün H, Yılmaz H (2002). Frequency of Escherichia coli O157:H7 in Turkish cattle. J Food Protect, 65, 1637-1640. Zhou Z, Nishikawa Y, Zhu P, Hong S, Hase A, Cheasty T, Smith HR, Zheng M, Haruki K (2002). Isolation and characterization of shiga toxinproducing Escherichia coli O157:H7 from beef, pork and cattle fecal samples in Changchun, China. J Vet Med Sci, 64, 1041-1044.
Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Hakan Kalender

Yayımlanma Tarihi 20 Aralık 2016
Gönderilme Tarihi 19 Ağustos 2016
Kabul Tarihi 10 Kasım 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 27 Sayı: 3

Kaynak Göster

APA Kalender, H. (2016). Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması. Van Veterinary Journal, 27(3), 161-164.
AMA Kalender H. Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması. Van Vet J. Aralık 2016;27(3):161-164.
Chicago Kalender, Hakan. “Sütçü Sığırlardan İzole Edilen Escherichia Coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması”. Van Veterinary Journal 27, sy. 3 (Aralık 2016): 161-64.
EndNote Kalender H (01 Aralık 2016) Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması. Van Veterinary Journal 27 3 161–164.
IEEE H. Kalender, “Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması”, Van Vet J, c. 27, sy. 3, ss. 161–164, 2016.
ISNAD Kalender, Hakan. “Sütçü Sığırlardan İzole Edilen Escherichia Coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması”. Van Veterinary Journal 27/3 (Aralık 2016), 161-164.
JAMA Kalender H. Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması. Van Vet J. 2016;27:161–164.
MLA Kalender, Hakan. “Sütçü Sığırlardan İzole Edilen Escherichia Coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması”. Van Veterinary Journal, c. 27, sy. 3, 2016, ss. 161-4.
Vancouver Kalender H. Sütçü Sığırlardan İzole Edilen Escherichia coli O157:H7 Suşlarında Virulans Genlerinin Varlığının Araştırılması. Van Vet J. 2016;27(3):161-4.

88x31.png

Kabul edilen makaleler Creative Commons Atıf-Ticari Olmayan Lisansla Paylaş 4.0 uluslararası lisansı ile lisanslanmıştır.