Araştırma Makalesi
Tavşanlarda Mistletoe Lektin-I’in Farklı Dozlarının Tümör Nekroz Faktör-α, Nitrik Oksit, Total Antioksidan ve Oksidan Kapasite Düzeylerine Etkisi
Öz
Mistletoe
lektin-I (ML-I)’in, farklı dozlarının tümör nekroz faktör-α (TNF-α), nitrik
oksit (NO), total antioksidan ve oksidan kapasite (TAK, TOK) düzeylerine
etkisinin araştırıldığı çalışmada, her birinde 7 adet tavşan bulunan, birisi
kontrol olmak üzere 4 grup oluşturuldu. Gruplara sırasıyla 0.5, 1 ve 2 ng/kg
ML-I intraperitonal (i.p.) olarak enjekte edildi. Enjeksiyonu takiben 1., 6.,
12. ve 24. saatlerde kan örnekleri alınarak plazmaları elde edildi. 0.5 ng/kg
ML-I verilen grupta TNF-α düzeyinin bütün saatlerde kontrol ve diğer lektin
gruplarına göre yüksek olmakla birlikte 1. saatte en yüksek değerine ulaştığı
(p<0.01) saptandı. 0.5 ve 1 ng/kg lektin dozlarının 1. saatte NO düzeyini
azalttığı (p<0.05), 1 ve 2 ng/kg lektin dozlarının 12. saatte NO düzeyini
arttırdığı (p<0.05), gruplar arasında istatistiksel olarak bir farkın
oluşmadığı gözlendi. 0.5 ng/kg ML-I verilen gruptan alınan numunelerde, 1 saat
sonra elde edilen TAK değerlerinin 6, 12 ve 24. saatlere göre istatistiksel
olarak yüksek (p<0.05) olduğu, TOK düzeylerinde istatistiksel olarak bir
değişim olmadığı gözlemlendi. 0.5 ng/kg dozundaki ML-I’in TNF-α ve NO
düzeylerinde değişiklik meydana getirmesi,
lektinin akut durumlarda antioksidan özellik gösterebileceği kanaatini
oluşturdu.
Anahtar Kelimeler
Mistletoe Lektin-I TNF-α Nitrik Oksit Total Antioksidan ve Oksidan Kapasite
Kaynakça
- Bantel H, Engels IH. Voelter W, Schulze-Osthoff K, Wesselborg S (1999). Mistletoe lectin activates caspase-8/FLICE independently of death receptor signaling and enhances anticancer drug-induced apoptosis. Cancer Res, 59, 2083-2090. Barbasz A, Kreczmer B, Rudolphi-Skorska E, Sieprawska A, Woznica D (2012). Content of antioxidant in extracts of mistletoe (Viscum album L.), yew (Taxus baccata L.), pine (Pinus sylvestris L.) and fir (Abies alba Mill.). Herba Polonica, 58, 27-36. Beuth J, Stoffel B, Ko HL, Jeljaszewicz J, Pulverer G (1995). Immunomodulating ability of galactoside-specific lectin standardized and depleted mistletoe extract. Arzneimittel-Forschung: Drug Res, 45, 1240-1242. Biberstein SE, Spiro JD, Lindquist R, Kreutzer DL (1995). Enhanced tumor cell expression of tumor necrosis factor receptors in head and neck squamous cell carcinoma. AM J Surg, 170, 416-422. Boneberg E-M, Hartung T(2001). Mistletoe lectin-I increases tumor necrosis factor-α release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production. J Pharm Exp Therap, 298 (3), 996-1000. Büssing A, Suzart K, Schweizer K (1997). Differences in the apoptosis-inducing properties of Viscum album L. extracts. Anticancer Drugs Suppl, 1, 9-14. Büssing A, Suzart K, Pfüller U, Schietzel M (1999). Induction of Fas ligand (CD95L) by the toxic mistletoe lectins in human lymphocytes. Anticancer Res, 19, 1785-1790. Cebovic T, Popovic M (2006). Effects of different extracts of mistletoe leaves ( Viscum album L.) on CCl4-induced hepatotoxicity in rats. Tox Lett (Abs.), 164-174. Danjolli D, Akşit H (2016). Effects of N acetylcysteine on expression and localization of some lectins in the liver of experimental hepatic intoxication. Van Vet J, 11-16. Finkel T (1998). Oxygen radicals and signaling. Curr Opin Cell Biol, 10, 248-253. Franz H, Ziska P, Kindt A (1981). Isolation and properties of three lectins from mistletoe (Viscum album L.). Biochem J, 195, 481-484. Hajto T, Hostanska K, Frei K, Rordorf C, Gabius H-J (1990). Increased secretion of tumor necrosis factor α. interleukin I and interleukin 6 by human mononuclear cells exposed to β-galactoside-specific lectin from clinically applied mistletoe extract. Cancer Res, 50, 3322-3326. Hajto T, Hostanska K, Fischer J, Saller R (1997). Immunomodulatory effects of Viscum album agglutinin-I on natural immunity. Anticancer Drugs, 8 (Suppl 1), 43-46. Hajto T, Hostanska K, Berki T, Palinkas L, Boldizsar F, Nemeth P (2005). Oncopharmacological perspectives of a plant lectin (Viscum album Agglutinin-I): overview of recent results from in vitro experiments and in vivo animal models, and their possible relevance for clinical applications. eCAM, 2, 59-67. Ignarro LJ (2010). Nitric oxide, Biology and Pathobiology. 2nd Ed. Academic Press Publications, USA. Kang TB, Yoo YC, Lee KH, et al. (2008). Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. J Biochem Mol Biol, 15, 197-204. Kim BK, Choi MJ, Park KY, Cho EJ (2010). Protective effects of Korean mistletoe lectin on radical-induced oxidative stres. Biol Pharm Bull, 33(7), 1152-1158. Kim W-H, Park WB, Gao B, Jung MH (2004). Critical role of reactive oxygen species and mitocondrial membrane potential in Korean mistletoe lectin-induced apoptosis in human hepatocarcinoma cells. Mol Pharmacol, 66, 1383-1396. Kurokawa H, Takeda S, Yamashita Y, et al. (2002). Estimation of Serum tumour necrosis factor-α and correlation to tumour markers in patients with oral squamous cell carcinoma. Asian J Oral Maxillofac Surg, 14, 148-154. Lee JY, Kim JY, Lee YG, et al. (2007). In vitro immunoregulatory effects of Korean mistletoe lectin on functional activation of monocytic and macrophage-like cells. Biol Pharm Bull, 30, 2043-2051. Leu Y-L, Hwang T-L, Chung YM, Hong PY (2006). The Inhibition of superoxide anion generation in human neutrophils by Viscum coloratum. Chem Pharmacol Bull, 54(7), 1063-1066. Lyu SY, Choi SH, Park WB (2002). Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerase via mitochondrial controlled pathway independent of p53. Arch Pharm Res, 25, 93-101. Mannel DN, Becker H, Gundt A, Kist A, Franz H (1991). Induction of tumor necrosis factor expression by a lectin from Viscum album. Cancer Immunol Immunotherapy, 33, 177-182. Miranda KM, Espey MG, Wink DA (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide Biol Chem, 5, 62-71. Nwanjo HU (2007). Free radical scavenging potential of the aqueous extract of Viscum album (Mistletoe) leaves in diabetic Wistar rats hepatocytes. Internet J Nutr Wellness, 3(2), 1-8 Odeh M (1990). The role of tumour necrosis factor-α in acquired immunodeficiency syndrome. J Int Med, 228, 549-556. Oluwaseun AA, Ganiyu O (2008). Antioxidant properties of methanolic extracts of mistletoes (Viscum album) from cocoa and cashew trees in Nigeria. African J Biotecnol, 7, 3138-3142. Orhan DD, Aslan M, Şendoğdu N, Ergun F, Yeşilada E (2005). Evaluation of hypoglycemic effect and antioxidant activity of three Viscum album supspecies (European mistletoe) in streptozotocin-diabetic rats. J Ethnopharm 98, 95-102. Papuc C, Crivineanu M, Goran G, Nicorescu V, Durdun N (2010). Free radicals scavenging and antioxidant activity of European mistletoe (Viscum album) and European birthwort (Aristolochia clematitis). Rev Chim, 61, 619-622. Park JH, Hyun, CK, Shin HK (1999). Cytotoxic effects of the components in heat-treated mistletoe. Cancer Lett, 139, 207-213. Ribereau-Gayon G, Dumont S, Muller C, Jung ML, Poindron P, Anton R (1996). Mistletoe lectins I, II and III induce the production of cytokines by cultured human monocytes. Cancer Lett, 109, 33-38. Roman GP, Neagu E, Radu GL (2009). Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process. Acta Sci Pol Technol Aliment, 8, 47-58. Sandes EO, Faletti AG, Riveros MD, et al. (2005). Expression of inducible nitric oxide synthase in tumoral and non-tumoral epithelia from bladder cancer patients. Nitric Oxide, 12, 39-45. Shi Z-M, Feng P, Jiang DQ, Wang XJ (2006). Mistletoe alkali inhibits peroxidation in rat liver and kidney. World J Gastrenterol, 12(25), 4052-4055. Stirpe F, Legg RF, Onyon LJ, Ziska P, Franz H (1980). Inhibition of protein synthesis by a toxic lectin from Viscum album L. (mistletoe). Biochem J, 190, 843-845. Sung, N-Y, Byun, E-B., Song, D-S., et al. (2013). Effect of gamma irradiation on mistletoe (Viscum album) lectin-mediated toxicity and immunomodulatory activity. FEBS Open Bio, 3,106-111. Timoshenko AV, Gabius H-J (1993). Efficient induction of superoxide release from human neutrophils by the galactoside galactoside specific lectin from Viscum album. Biol. Chem Hoppe-Seyler, 374,237-43. Timoshenko AV, Cherenkevich SN, Gabius H-J (1995). Viscum album agglutinin-induced aggregation of blood cells and lectin effect on neutrophil. Biomed Pharmcol, 49, 153-158. Timoshenko AV, Lan Y, Gabius H-J, Lala PK (2001). Immunotherapy of C3H/Hej mammary adenocarcinoma with interleukin-2, mistletoe lectin or their combination: effects on tumor growth, capillary leakage and nitric oxide (NO) production. Eur J Cancer, 37, 1910-1920. Ziska P, Eifler R, Franz H (1979). Chemical modification studies on the D-galactopyranosyl binding lectin from the mistletoe Viscum album L. Acta Biol Med Ger, 38(9), 1361-1363. in: Ergun F, Deliorman D, Şener B(1995). Viscum album L. Lektinleri. FABAD J Pharmacol Sci, 20, 117-123. Zorlu S, Gün H, Demirbağ E, Çınar K (2013). Sığır jejenum mukozasındaki bazı glikokonjugatların lektin histokimyasal karakterizasyonu. Van Vet J, 73-75.
Effect of Different Doses of Mistletoe Lectin-I on the Levels of Tumor Necrosis Factor-α, Nitric Oxide, Total Antioxidant and Oxidant Capacity in Rabbits
Öz
This
paper aimed to investigate the effect of different doses of mistletoe lectin-I
(ML-I) on the levels of tumor necrosis factor-α (TNF-α), nitric oxide (NO),
total antioxidant and oxidant capacity (TAC, TOC). The rabbits were divided
into 4 groups (Control, Group-I, II and III) each group contained 7
rabbits. 0.5, 1 and 2 ng/kg of ML-I were
administered to treatment groups via intraperitoneal route, respectively.
Plasmas of blood samples obtained in the 1st, 6st, 12th
and 24th hours following injection were seperated. TNF-α level in
the group treated with ML-I (0.5 ng/kg) was higher than that of control and
other lectin receiving groups at all hours, the highest value of TNF-α was
reached at 1st hour (p<0.01). 0.5 and 1 ng/kg doses of lectin
decreased NO level at 1st hour compared to control group
(p<0.05), 1 and 2 ng/kg doses of lectin increased NO levels (p<0.05) at
12th hour, no statistically significant difference was found between
the groups. In addition, TAC levels in samples
obtained from the group given 0.5 ng/kg ML-I after 1 hour was statistically
higher (p<0.05) than the samples taken at 6th, 12th
and 24th hours. No statistically significant change was found in the
level of TOC among lectin receiving groups. In conclusion, ML-I at 0.5 ng/kg
dose caused alterations in the levels of TNF-α and NO and showed an acute
antioxidant effect.
Anahtar Kelimeler
Mistletoe Lectin-I TNF-α Nitric Oxide Total Antioxidant and Oxidant Capacity
Kaynakça
- Bantel H, Engels IH. Voelter W, Schulze-Osthoff K, Wesselborg S (1999). Mistletoe lectin activates caspase-8/FLICE independently of death receptor signaling and enhances anticancer drug-induced apoptosis. Cancer Res, 59, 2083-2090. Barbasz A, Kreczmer B, Rudolphi-Skorska E, Sieprawska A, Woznica D (2012). Content of antioxidant in extracts of mistletoe (Viscum album L.), yew (Taxus baccata L.), pine (Pinus sylvestris L.) and fir (Abies alba Mill.). Herba Polonica, 58, 27-36. Beuth J, Stoffel B, Ko HL, Jeljaszewicz J, Pulverer G (1995). Immunomodulating ability of galactoside-specific lectin standardized and depleted mistletoe extract. Arzneimittel-Forschung: Drug Res, 45, 1240-1242. Biberstein SE, Spiro JD, Lindquist R, Kreutzer DL (1995). Enhanced tumor cell expression of tumor necrosis factor receptors in head and neck squamous cell carcinoma. AM J Surg, 170, 416-422. Boneberg E-M, Hartung T(2001). Mistletoe lectin-I increases tumor necrosis factor-α release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production. J Pharm Exp Therap, 298 (3), 996-1000. Büssing A, Suzart K, Schweizer K (1997). Differences in the apoptosis-inducing properties of Viscum album L. extracts. Anticancer Drugs Suppl, 1, 9-14. Büssing A, Suzart K, Pfüller U, Schietzel M (1999). Induction of Fas ligand (CD95L) by the toxic mistletoe lectins in human lymphocytes. Anticancer Res, 19, 1785-1790. Cebovic T, Popovic M (2006). Effects of different extracts of mistletoe leaves ( Viscum album L.) on CCl4-induced hepatotoxicity in rats. Tox Lett (Abs.), 164-174. Danjolli D, Akşit H (2016). Effects of N acetylcysteine on expression and localization of some lectins in the liver of experimental hepatic intoxication. Van Vet J, 11-16. Finkel T (1998). Oxygen radicals and signaling. Curr Opin Cell Biol, 10, 248-253. Franz H, Ziska P, Kindt A (1981). Isolation and properties of three lectins from mistletoe (Viscum album L.). Biochem J, 195, 481-484. Hajto T, Hostanska K, Frei K, Rordorf C, Gabius H-J (1990). Increased secretion of tumor necrosis factor α. interleukin I and interleukin 6 by human mononuclear cells exposed to β-galactoside-specific lectin from clinically applied mistletoe extract. Cancer Res, 50, 3322-3326. Hajto T, Hostanska K, Fischer J, Saller R (1997). Immunomodulatory effects of Viscum album agglutinin-I on natural immunity. Anticancer Drugs, 8 (Suppl 1), 43-46. Hajto T, Hostanska K, Berki T, Palinkas L, Boldizsar F, Nemeth P (2005). Oncopharmacological perspectives of a plant lectin (Viscum album Agglutinin-I): overview of recent results from in vitro experiments and in vivo animal models, and their possible relevance for clinical applications. eCAM, 2, 59-67. Ignarro LJ (2010). Nitric oxide, Biology and Pathobiology. 2nd Ed. Academic Press Publications, USA. Kang TB, Yoo YC, Lee KH, et al. (2008). Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. J Biochem Mol Biol, 15, 197-204. Kim BK, Choi MJ, Park KY, Cho EJ (2010). Protective effects of Korean mistletoe lectin on radical-induced oxidative stres. Biol Pharm Bull, 33(7), 1152-1158. Kim W-H, Park WB, Gao B, Jung MH (2004). Critical role of reactive oxygen species and mitocondrial membrane potential in Korean mistletoe lectin-induced apoptosis in human hepatocarcinoma cells. Mol Pharmacol, 66, 1383-1396. Kurokawa H, Takeda S, Yamashita Y, et al. (2002). Estimation of Serum tumour necrosis factor-α and correlation to tumour markers in patients with oral squamous cell carcinoma. Asian J Oral Maxillofac Surg, 14, 148-154. Lee JY, Kim JY, Lee YG, et al. (2007). In vitro immunoregulatory effects of Korean mistletoe lectin on functional activation of monocytic and macrophage-like cells. Biol Pharm Bull, 30, 2043-2051. Leu Y-L, Hwang T-L, Chung YM, Hong PY (2006). The Inhibition of superoxide anion generation in human neutrophils by Viscum coloratum. Chem Pharmacol Bull, 54(7), 1063-1066. Lyu SY, Choi SH, Park WB (2002). Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerase via mitochondrial controlled pathway independent of p53. Arch Pharm Res, 25, 93-101. Mannel DN, Becker H, Gundt A, Kist A, Franz H (1991). Induction of tumor necrosis factor expression by a lectin from Viscum album. Cancer Immunol Immunotherapy, 33, 177-182. Miranda KM, Espey MG, Wink DA (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide Biol Chem, 5, 62-71. Nwanjo HU (2007). Free radical scavenging potential of the aqueous extract of Viscum album (Mistletoe) leaves in diabetic Wistar rats hepatocytes. Internet J Nutr Wellness, 3(2), 1-8 Odeh M (1990). The role of tumour necrosis factor-α in acquired immunodeficiency syndrome. J Int Med, 228, 549-556. Oluwaseun AA, Ganiyu O (2008). Antioxidant properties of methanolic extracts of mistletoes (Viscum album) from cocoa and cashew trees in Nigeria. African J Biotecnol, 7, 3138-3142. Orhan DD, Aslan M, Şendoğdu N, Ergun F, Yeşilada E (2005). Evaluation of hypoglycemic effect and antioxidant activity of three Viscum album supspecies (European mistletoe) in streptozotocin-diabetic rats. J Ethnopharm 98, 95-102. Papuc C, Crivineanu M, Goran G, Nicorescu V, Durdun N (2010). Free radicals scavenging and antioxidant activity of European mistletoe (Viscum album) and European birthwort (Aristolochia clematitis). Rev Chim, 61, 619-622. Park JH, Hyun, CK, Shin HK (1999). Cytotoxic effects of the components in heat-treated mistletoe. Cancer Lett, 139, 207-213. Ribereau-Gayon G, Dumont S, Muller C, Jung ML, Poindron P, Anton R (1996). Mistletoe lectins I, II and III induce the production of cytokines by cultured human monocytes. Cancer Lett, 109, 33-38. Roman GP, Neagu E, Radu GL (2009). Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process. Acta Sci Pol Technol Aliment, 8, 47-58. Sandes EO, Faletti AG, Riveros MD, et al. (2005). Expression of inducible nitric oxide synthase in tumoral and non-tumoral epithelia from bladder cancer patients. Nitric Oxide, 12, 39-45. Shi Z-M, Feng P, Jiang DQ, Wang XJ (2006). Mistletoe alkali inhibits peroxidation in rat liver and kidney. World J Gastrenterol, 12(25), 4052-4055. Stirpe F, Legg RF, Onyon LJ, Ziska P, Franz H (1980). Inhibition of protein synthesis by a toxic lectin from Viscum album L. (mistletoe). Biochem J, 190, 843-845. Sung, N-Y, Byun, E-B., Song, D-S., et al. (2013). Effect of gamma irradiation on mistletoe (Viscum album) lectin-mediated toxicity and immunomodulatory activity. FEBS Open Bio, 3,106-111. Timoshenko AV, Gabius H-J (1993). Efficient induction of superoxide release from human neutrophils by the galactoside galactoside specific lectin from Viscum album. Biol. Chem Hoppe-Seyler, 374,237-43. Timoshenko AV, Cherenkevich SN, Gabius H-J (1995). Viscum album agglutinin-induced aggregation of blood cells and lectin effect on neutrophil. Biomed Pharmcol, 49, 153-158. Timoshenko AV, Lan Y, Gabius H-J, Lala PK (2001). Immunotherapy of C3H/Hej mammary adenocarcinoma with interleukin-2, mistletoe lectin or their combination: effects on tumor growth, capillary leakage and nitric oxide (NO) production. Eur J Cancer, 37, 1910-1920. Ziska P, Eifler R, Franz H (1979). Chemical modification studies on the D-galactopyranosyl binding lectin from the mistletoe Viscum album L. Acta Biol Med Ger, 38(9), 1361-1363. in: Ergun F, Deliorman D, Şener B(1995). Viscum album L. Lektinleri. FABAD J Pharmacol Sci, 20, 117-123. Zorlu S, Gün H, Demirbağ E, Çınar K (2013). Sığır jejenum mukozasındaki bazı glikokonjugatların lektin histokimyasal karakterizasyonu. Van Vet J, 73-75.
Toplam 1 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 20 Nisan 2017 |
| Gönderilme Tarihi | 12 Ocak 2017 |
| Kabul Tarihi | 4 Nisan 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 28 Sayı: 1 |
Kaynak Göster
Kabul edilen makaleler Creative Commons Atıf-Ticari Olmayan Lisansla Paylaş 4.0 uluslararası lisansı ile lisanslanmıştır.