Öz
Bu çalışmada 17 β östradiol, Dietilsitilbesterol ve progesteron gibi steroid hormonların F98 glioblostomo hücre hatlarında hücre canlılığı üzerine etkilerinin araştırılması amaçlanmıştır. F98 glioblastoma hücrelerine farlı dozlarda Progesteron (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) ve 17β estradiol (0.01, 0.1, 1, 10 μM) 24, 48 ve 72 saat süre ile uygulanmış ve hücre canlılığının belirlenmesi amacıyla MTT hücre canlılık testi uygulanmıştır. Progesteron glioblostoma hücrelerinin büyümesini doz ve zaman bağımlı olarak inhibe etmiştir. 17 β estradiol düşük dozlarda antiproliferatif etki göstermiştir. DES uygulamaları hücre canlılığı üzerinde iki yönlü etki göstermiştir. Elde edilen sonular Progesteron, 17β estradiol ve DES’in glioblostoma hücrelerinde hücre çoğalmasını inhibe ettiğini göstermektedir. Ancak bununla ilgili yolakların belirlenmesi için daha fazla çalışma yapılmasına ihtiyaç vardır.
Anahtar Kelimeler
Kaynakça
- REFFERENCESAli Shah SI (2015). Emerging potential of parenteral estrogen as androgen deprivation therapy for prostate cancer. South Asian J Cancer, 4(2), 95–97. doi:10.4103/2278-330X.155699
- Altiok N, Ersoz M, Koyuturk M (2011). Estradiol induces JNK-dependent apoptosis in glioblastoma cells. Oncol Lett, 2(6), 1261-1285.
- Atif F, Patel NR, Yousuf S, Stein DG (2015). The Synergistic Effect of Combination Progesterone and Temozolomide on Human Glioblastoma Cells. PLoS ONE 10(6): e0131441. https://doi.org/10.1371/journal.pone.0131441
- Atif F, Yousuf S, Stein DG (2015). Anti-tumor effects of progesterone in human glioblastoma multiforme: Role of PI3K/Akt/mTOR signaling. J Steroid Biochem Mol Biol, 146, 62-73.
- Cabrera-Muñoz E, Hernández-Hernández OT, Camacho-Arroyo I (2011). Role of progesterone in human astrocytoma growth. Curr Top Med Chem, 11(13), 1663–7.
- Ding H, Wu X, Roncari L, Lau N, Shannon P, Nagy A, Guha A (2000). Expression and regulation of neuropilin-1 in human astrocytomas. Int J Cancer, 88, 584–592.
- Dueñas Jiménez JM, Candanedo Arellano A, Santerre A, Orozco Suárez S, Sandoval Sánchez H, Feria Romero I, López-Elizalde R, Alonso Venegas M, Netel B, de la Torre Valdovinos B, Dueñas Jiménez SH (2014). Aromatase and estrogen receptor alpha mRNA expression as prognostic biomarkers in patients with astrocytomas. J Neurooncol, 119(2), 275–84.
- Ho VK, Reijneveld JC, Enting RH, Bienfait HP, Robe P, Baumert BG, et al. (2014) Changing incidence and improved survival of gliomas. Eur J Cancer, 50(13), 2309–18.
- IARC (2012). International Agency for Research on Cancer. Pharmaceuticals. Diethylstilbestrol. A review of human carcinogens. IARC Monogr Eval Carcinog Risks Hum, 100A:175–218
- Kabat GC, Etgen AM, Rohan TE (2010). Do Steroid Hormones Play a Role in the Etiology of Glioma? Cancer Epidemiol Biomarkers Prev, 19(10), 2421–7.
- Korach KS, Metzler M, McLachlan JA (1978). Estrogenic activity in vivo and in vitro of some diethylstilbestrol metabolites and analogs. Proc Natl Acad Sciences, 75, 468–71.
- Marceau K, Ruttle PL, Shirtcliff EA, Essex MJ, Susman EJ (2015). Developmental and contextual considerations for adrenal and gonadal hormone functioning during adolescence: Implications for adolescent mental health. Dev. Psychobiol, 57, 742–768.
- Miller WL, Bose HS (2011). Early steps in steroidogenesis: Intracellular cholesterol trafficking. J. Lipid Res, 52, 2111–2135.
- Patel S, Dibiase S, Meisenberg B, Flannery T, Patel A, Dhople A, et al (2012). Phase I clinical trial assessing temozolomide and tamoxifen with concomitant radiotherapy for treatment of high-grade glioma. Int J Radiat Oncol Biol Phys, 82(2), 739–42.
- Piña-Medina AG, Hansberg-Pastor V, González-Arenas A, Cerbón M, Camacho-Arroyo I (2016). Progesterone promotes cell migration, invasion and cofilin activation in human astrocytoma cells. Steroids, 105, 19-25.
- Rossetti MF, Cambiasso MJ, Holschbach MA, Cabrera R (2016). Oestrogens and Progestagens: Synthesis and Action in the Brain. J Neuroendocrinol, 28(7), 1-11.
- Schumacher M, Weill-Engerer S, Liere P, Robert F, Franklin RJ, Garcia-Segura LM, Lambert JJ, Mayo W, Melcangi RC, Parducz A, Suter U, Carelli C, Baulieu EE, Akwa Y (2003). Steroid hormones and neurosteroids in normal and pathological aging of the nervous system. Prog Neurobiol, 71, 3–29.
- Tang P, Roldan G, Brasher PM, Fulton D, Roa W, Murtha A, et al (2006). A phase II study of carboplatin and chronic high-dose tamoxifen in patients with recurrent malignant glioma. J Neurooncol, 78(3), 311–6.
- Tavares CB, Gomes-Braga FC, Costa-Silva DR, Esco´rcio-Dourado CS, Borges US, Conde-Junior AM, Barros-Oliveira MC, Sousa EB, Barros LR, Martins LM, Facina G, Silva BB (2016). Expression of estrogen and progesterone cs in astrocytomas: a literature review. Clinics, 71(8), 481-486.
- Virgil E. J. C. Schijns, Chrystel Pretto, Anna M. Strik, Rianne Gloudemans-Rijkers, Laurent Devillers, Denis Pierre, Jinah Chung, Manisha Dandekar, Jose A. Carrillo, Xiao-Tang Kong, Beverly D. Fu, Frank P. K. Hsu, Florence M. Hofman, Thomas C. Chen, Raphael Zidovetzki, Daniela A. Bota, Apostolos Stathopoulos (2018). Therapeutic Immunization against Glioblastoma. Int. J. Mol. Sci. 19, 2540
Öz
The aim of the present study is to investigate the effects of three different steroid hormones; 17 β estradiol, Diethylstilbestrol and progesterone on cell viability in F98 glioblastoma cells. F98 glioblastoma cells were treated with different concentrations of Progesterone (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) and 17β estradiol (0.01, 0.1, 1, 10 μM)) for 24, 48 and 72 hours and MTT assay was applied to determine thecell viability. Progesterone inhibits glioblastoma cell growth in a dose and time dependent manner. Antiproliferative effect of 17 β estradiol was observed at low doses. Biphasic distribution was observed in decreasing cell viability in DES applications. These results suggest that Progesterone, 17β estradiol and DES can inhibit the proliferation of glioblastoma cells. However, further study is necessary to identify the pathways involved.Bu çalışmada 17 β östradiol, Dietilsitilbesterol ve
progesterone gibi steroid hormonların F98 glioblostomo hücre hatlarında hücre
canlılığı üzerine etkilerinin araştırılması amaçlanmıştır. F98 glioblastoma hücrelerine farlı
dozlarda Progesteron (10, 20, 50, 100
μM), DES (2.5, 5, 10, 20, 50, 100 μM) ve 17β estradiol (0.01, 0.1, 1, 10 μM)
24, 48 ve 72 saat süre ile uygulanmış ve hücre canlılığının belirlenmesi
amacıyla MTT hücre canlılık testi uygulanmıştır. Progesteron glioblostoma
hücrelerinin büyümesini doz ve zaman bağımlı olarak inhibe etmiştir. 17 β estradiol düşük dozlarda
antiproliferatif etki göstermiştir. DES uygulamaları hücre canlılığı üzerinde
iki yönlü etki göstermiştir. Elde edilen sonular Progesterone, 17β estradiol ve DES’in glioblostoma hücrelerinde hücre
çoğalmasını inhibe ettiğini göstermektedir. Ancak bununla ilgili yolakların
belirlenmesi için daha fazla çalışma yapılmasına ihtiyaç vardır.
Anahtar Kelimeler
Kaynakça
- REFFERENCESAli Shah SI (2015). Emerging potential of parenteral estrogen as androgen deprivation therapy for prostate cancer. South Asian J Cancer, 4(2), 95–97. doi:10.4103/2278-330X.155699
- Altiok N, Ersoz M, Koyuturk M (2011). Estradiol induces JNK-dependent apoptosis in glioblastoma cells. Oncol Lett, 2(6), 1261-1285.
- Atif F, Patel NR, Yousuf S, Stein DG (2015). The Synergistic Effect of Combination Progesterone and Temozolomide on Human Glioblastoma Cells. PLoS ONE 10(6): e0131441. https://doi.org/10.1371/journal.pone.0131441
- Atif F, Yousuf S, Stein DG (2015). Anti-tumor effects of progesterone in human glioblastoma multiforme: Role of PI3K/Akt/mTOR signaling. J Steroid Biochem Mol Biol, 146, 62-73.
- Cabrera-Muñoz E, Hernández-Hernández OT, Camacho-Arroyo I (2011). Role of progesterone in human astrocytoma growth. Curr Top Med Chem, 11(13), 1663–7.
- Ding H, Wu X, Roncari L, Lau N, Shannon P, Nagy A, Guha A (2000). Expression and regulation of neuropilin-1 in human astrocytomas. Int J Cancer, 88, 584–592.
- Dueñas Jiménez JM, Candanedo Arellano A, Santerre A, Orozco Suárez S, Sandoval Sánchez H, Feria Romero I, López-Elizalde R, Alonso Venegas M, Netel B, de la Torre Valdovinos B, Dueñas Jiménez SH (2014). Aromatase and estrogen receptor alpha mRNA expression as prognostic biomarkers in patients with astrocytomas. J Neurooncol, 119(2), 275–84.
- Ho VK, Reijneveld JC, Enting RH, Bienfait HP, Robe P, Baumert BG, et al. (2014) Changing incidence and improved survival of gliomas. Eur J Cancer, 50(13), 2309–18.
- IARC (2012). International Agency for Research on Cancer. Pharmaceuticals. Diethylstilbestrol. A review of human carcinogens. IARC Monogr Eval Carcinog Risks Hum, 100A:175–218
- Kabat GC, Etgen AM, Rohan TE (2010). Do Steroid Hormones Play a Role in the Etiology of Glioma? Cancer Epidemiol Biomarkers Prev, 19(10), 2421–7.
- Korach KS, Metzler M, McLachlan JA (1978). Estrogenic activity in vivo and in vitro of some diethylstilbestrol metabolites and analogs. Proc Natl Acad Sciences, 75, 468–71.
- Marceau K, Ruttle PL, Shirtcliff EA, Essex MJ, Susman EJ (2015). Developmental and contextual considerations for adrenal and gonadal hormone functioning during adolescence: Implications for adolescent mental health. Dev. Psychobiol, 57, 742–768.
- Miller WL, Bose HS (2011). Early steps in steroidogenesis: Intracellular cholesterol trafficking. J. Lipid Res, 52, 2111–2135.
- Patel S, Dibiase S, Meisenberg B, Flannery T, Patel A, Dhople A, et al (2012). Phase I clinical trial assessing temozolomide and tamoxifen with concomitant radiotherapy for treatment of high-grade glioma. Int J Radiat Oncol Biol Phys, 82(2), 739–42.
- Piña-Medina AG, Hansberg-Pastor V, González-Arenas A, Cerbón M, Camacho-Arroyo I (2016). Progesterone promotes cell migration, invasion and cofilin activation in human astrocytoma cells. Steroids, 105, 19-25.
- Rossetti MF, Cambiasso MJ, Holschbach MA, Cabrera R (2016). Oestrogens and Progestagens: Synthesis and Action in the Brain. J Neuroendocrinol, 28(7), 1-11.
- Schumacher M, Weill-Engerer S, Liere P, Robert F, Franklin RJ, Garcia-Segura LM, Lambert JJ, Mayo W, Melcangi RC, Parducz A, Suter U, Carelli C, Baulieu EE, Akwa Y (2003). Steroid hormones and neurosteroids in normal and pathological aging of the nervous system. Prog Neurobiol, 71, 3–29.
- Tang P, Roldan G, Brasher PM, Fulton D, Roa W, Murtha A, et al (2006). A phase II study of carboplatin and chronic high-dose tamoxifen in patients with recurrent malignant glioma. J Neurooncol, 78(3), 311–6.
- Tavares CB, Gomes-Braga FC, Costa-Silva DR, Esco´rcio-Dourado CS, Borges US, Conde-Junior AM, Barros-Oliveira MC, Sousa EB, Barros LR, Martins LM, Facina G, Silva BB (2016). Expression of estrogen and progesterone cs in astrocytomas: a literature review. Clinics, 71(8), 481-486.
- Virgil E. J. C. Schijns, Chrystel Pretto, Anna M. Strik, Rianne Gloudemans-Rijkers, Laurent Devillers, Denis Pierre, Jinah Chung, Manisha Dandekar, Jose A. Carrillo, Xiao-Tang Kong, Beverly D. Fu, Frank P. K. Hsu, Florence M. Hofman, Thomas C. Chen, Raphael Zidovetzki, Daniela A. Bota, Apostolos Stathopoulos (2018). Therapeutic Immunization against Glioblastoma. Int. J. Mol. Sci. 19, 2540
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 12 Mart 2020 |
| Gönderilme Tarihi | 16 Eylül 2019 |
| Kabul Tarihi | 5 Mart 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 31 Sayı: 1 |
Kaynak Göster
Kabul edilen makaleler Creative Commons Atıf-Ticari Olmayan Lisansla Paylaş 4.0 uluslararası lisansı ile lisanslanmıştır.