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Şam Keçilerinde Farklı Yaşlarda Serum Biyokimyasal Profilinin ve Oksidan-Antioksidan Aktivitelerinin Belirlenmesi

Yıl 2024, Cilt: 19 Sayı: 1, 1 - 8, 26.04.2024
https://doi.org/10.17094/vetsci.1471422

Öz

Bu çalışmada, farklı yaşlardaki Şam keçilerinin biyokimyasal profillerindeki değişikliklerin ve oksidan-antioksidan aktivitelerin araştırılması amaçlandı. Araştırmaya 45 adet gebe olmayan farklı yaşlardaki Şam keçisi dahil edildi. Keçiler yaşlarına göre üç gruba ayrıldı: keçi yavruları ( - ≤ 6-aylık), genç keçiler (2-3-yaş) ve yaşlı keçiler (5-8-yaş). Serum örneklerinde biyokimyasal parametreler ve oksidan-antioksidan aktiviteler ticari kitler ve kolorimetrik yöntemler kullanılarak belirlendi. En düşük serum üre, kan üre azotu, aspartat aminotransferaz, total protein, albumin, globulin ve magnezyum seviyeleri keçi yavrularında gözlemlendi (P < .05). Glukoz, inorganik fosfor, non-esterifiye yağ asitleri, albumin/globulin ve alanin aminotransferaz konsantrasyonları keçilerde yaşla birlikte azaldı (P < .05). Serum total oksidan kapasitesi, total antioksidan kapasitesi ve oksidatif stres indeksi düzeyleri keçi yavrularında yaşlı keçilere göre yüksekti (P < .05). En yüksek serum glutatyon ve glutatyon peroksidaz aktiviteleri genç keçilerde gözlemlendi (P < .05). Keçi yavrularına kıyasla yaşlı keçilerde serum β-karoten ve bilirubin konsantrasyonlarında artış tespit edildi (P < .05). Buna karşın serum katalaz aktivitesi, arilesteraz, seruloplazmin ve ürik asit değerleri gruplar arasında farklılık göstermedi. Elde edilen sonuçlar, bu ırkın yaşla ilişkili sağlık ve beslenme durumunun izlenmesine ve referans değerlerin belirlenmesine katkı sağlayacaktır.

Kaynakça

  • 1. Gül S, Biçer O. Fattening performance and carcass characteristics of yearling Damascus male goat. J Agric Food Environ Anim Sci. 2021;2(2):116-133
  • 2. Antunović Z, Novaković K, Klir Z, et al. Blood metabolic profile and acid-base status of Istrian goats-a critically endangered Croatian goat-in relation to age. Vet Arhiv. 2020;90(1):27-38.
  • 3. Kandlur A, Satyamoorthy K, Gangadharan G. Oxidative stress in cognitive and epigenetic aging: a retrospective glance. Front Mol Neurosci. 2020;18(13):41.
  • 4. Demirci-Çekiç S, Özkan G, Avan AN, Uzunboy S, Çapanoğlu E, Apak R. Biomarkers of oxidative stress and antioxidant defense. J Pharm Biomed Anal. 2022;209(5):114477.
  • 5. Cartee GD. Influence of age on skeletal muscle glucose transport and glycogen metabolism. Med Sci Sports Exerc. 1994;26(5):577-585.
  • 6. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activatedoxygen toxicity in the blood. Am J Obstet Gynecol. 1979;135(3):372-376.
  • 7. Ali SS, Ahsan H, Zia MK, Siddiqui T, Khan FH. Understanding oxidants and antioxidants: Classical team with new players. J Food Biochem. 2020;44(3): 13145.
  • 8. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-1111.
  • 9. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37(4):277-285.
  • 10. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882-888.
  • 11. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-169.
  • 12. Aebi H. Catalase in vitro methods in Enzymology. New York, ABD Academic press; 1984.
  • 13. Suzuki JP, Katoh NA. A simple and cheap method for measuring serum vitamin A in cattle using only spectrophotometer. Jpn J Vet Sci. 1990;52(6):1281-1283.
  • 14. Sunderman FW, Nomoto S. Measurement of human serum ceruloplasmin by its p-phenylene-diamine oxidase activity. Clin Chem. 1970;16(11):903-910.
  • 15. Bozukluhan K, Merhan O, Gökçe Hİ, et al. Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected Sheeppox virus. Kafkas Univ Vet Fak Derg. 2018;24(3):437-444.
  • 16. Salar S, Baştan İ, Baştan A, Pekcan M, Sel T. Investigation of changes in metabolic parameters and paraoxonase-1 during the transition period in Turkish Saanen goats. Kafkas Univ Vet Fak Derg. 2018;24(1):117-122.
  • 17. Lashari MH, Ahmad I, Masood S, et al. Hematological and biochemical parameters of pregnant and lactating goats in Rangeland of Cholistan Desert, Bahawalpur, Pakistan. J Hellenic Vet Med Soc. 2021;72(1):2781-2786.
  • 18. Karaşahin T, Aksoy NH, Haydardedeoglu AE, et al. Serum cholesterol levels in Hair goats of Aksaray Region. Indian J Anim Res. 2019;53(1):63-66.
  • 19. Carlos MML, Leite JHGM, Chaves DF, et al. Blood parameters in the Morada Nova sheep: influence of age, sex and body condition score. J Anim Plant Sci. 2015;25(4):950-955.
  • 20. Kiran S, Bhutta AM, Khan BA, Durrani S, Ali M, Iqbal F. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan. Asian Pac J Trop Biomed. 2012;2(4):304-306.
  • 21. Mondal M, Prakash BS. Changes in plasma non‐esterified fatty acids, glucose and α‐amino nitrogen and their relationship with body weight and plasma growth hormone in growing buffaloes (Bubalus bubalis). J Anim Physiol Anim Nutr (Berl). 2004;88(6):223-228.
  • 22. Al‐Rukibat R, Ismail Z, Al‐Zghoul MB, Hananeh W. Establishment of reference intervals of selected blood biochemical parameters in Shami goats. Vet Clin Pathol. 2020;49(4):665-668.
  • 23. Piccione G, Casella S, Lutri L, Vazzana I, Ferrantelli F, Caola G. Reference values for some haematological, haematochemical, and electrophoretic parameters in the Girgentana goat. Turk J Vet Anim Sci. 2010;34(2):197-204.
  • 24. Njidda AA, Hassan IT, Olatunji EA. Haematological and biochemical parameters of goat of semi arid environment fed on natural grazing rangeland of northern Nigeria. J Agr Vet Sci. 2013;3(2):1-8.
  • 25. Gurgoze SY, Icen H. The influence of age on clinical biochemical parameters in pure-bred Arabian mares. Equine Vet J. 2010;30(10):569-574.
  • 26. Yokus B, Cakir DU, Kanay Z, Gulten T, Uysal E. Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. J Vet Med A Physiol Pathol Clin Med. 2006;53(6):271-276.
  • 27. Devrim AK, Elmaz Ö, Mamak N, Sudağıdan M. Alterations in some clinical biochemistry values of Honamlı and Native Hair goats during pubertal development. Vet Arhiv. 2015;85(6):647-656.
  • 28. Abdolvahabi S, Zaeemi M, Mohri M, Naserian AA. Age related changes in serum biochemical profile of Saanen goat kids during the first three months of life. Revue Méd Vét. 2016;167(3):106-112.
  • 29. Celi P. The role of oxidative stress in small ruminants' health and production. R Bras Zootec. 2010;39(Suppl):348-363.
  • 30. Yatoo MI, Kanwar MS, Wani SA, Dinesh K, Umesh D. Alteration of metabolic biomarkers and oxidative stress indices in pashmina (Changthangi) goats under climate change. Indian J Anim Sci. 2016;86(4):405-413.
  • 31. Miller JK, Brzezinska-Slebodzinska E, Madsen FC. Oxidative stress, antioxidants, and animal function. J Dairy Sci. 1993;76(9):2812-2823.
  • 32. Gaál T, Speake B, Mezes M, Noble R, Surai P et al. Antioxidant parameters and ageing in some animal species. Comp Haematol Int. 1996;6(4):208-213.
  • 33. Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med. 2013;65:244-253.
  • 34. Simsek O, Ekici H, Cinar M, Atmaca N, Arıkan S, Guner B. Heavy metal accumulation and oxidative-antioxidative status in angora goats depending on age. Fresenius Environ Bull. 2015;24(8):2659-2663.
  • 35. Tekeli H, Kıral F, Bildik A, Yılmaz M, Kaçamaklı Z. Effect of aging on enzymatic and non-enzymatic antioxidant status in Saanen goats. Vet Med Zoot. 2015;71(93):67-71.
  • 36. Stahl W, Sies H. β-Carotene and other carotenoids in protection from sunlight. Am J Clin Nutr. 2012;96(5):1179-1184.
  • 37. Vítek L. Bilirubin as a signaling molecule. Med Res Rev. 2020;40(4):1335-1351.
  • 38. Mohri M, Sharifi K, Eidi S. Hematology and serum biochemistry of Holstein dairy calves: age-related changes and comparison with blood composition in adults. Res Vet Sci. 2007;83(1):30-39.
  • 39. Gavare V, Kiran K, Kiran D, Sachin S. Ceruloplasmin its role and significance: a review. IJBR. 2010;1(4):153-162.
  • 40. Kartal YK, Kurt S, Ekşi F, Salar S, Kısmalı G et al. Alteration of paraoxonase, ceruloplasmin and immunoglobulin G levels in hair goats at different ages. J Adv VetBio Sci Tech. 2022;7(1):8-13.
  • 41. Sautin YY, Johnson RJ. Uric acid: the oxidant-antioxidant paradox. Nucleos Nucleot Nucl. 2008;27(6-7):608-619.
  • 42. Toker A, Kadı M, Yıldırım AK, Aksoy H, Akçay F. Serum lipid profile paraoxonase and arylesterase activities in psoriasis. Cell Biochem Funct. 2009;27(3):176-180.
  • 43. Taha N, El-Noeman S, Korshom M, Mandour A, El-Feky M. Serum glutathione peroxidase and paraoxonase-1 enzyme activities in camel (Camelus dromedarius): effect of age and sex differences. Camel Pract Res. 2010;17(1):1-8.

Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages

Yıl 2024, Cilt: 19 Sayı: 1, 1 - 8, 26.04.2024
https://doi.org/10.17094/vetsci.1471422

Öz

This study aimed to research changes in biochemical profile and oxidant-antioxidant activities of Damascus goats of different ages. The 45 non-gravid Damascus goats of different ages were included in the research. They were divided into three groups of 15 goats according to age: goat kids ( - ≤ 6-months age), young goats (2-3-year age) and old goats (5-8-year age). Biochemical parameters and oxidant-antioxidant activities were determined in serum samples using commercial kits and colorimetric methods. The lowest serum urea, blood urea nitrogen, aspartate aminotransferase, total protein, albumin, globulin and magnesium levels were observed in goat kids (P < .05). Concentrations of glucose, inorganic phosphorus, non-esterified fatty acids, albumin/globulin and alanine aminotransferase decreased with age in the goats (P < .05). Serum total oxidant capacity, total antioxidants capacity and oxidative stress index levels were increased in goat kids compared to old goats (P < .05). Furthermore the highest serum glutathione and glutathione preoxidase activities were observed in young goats (P < .05). An increase was determined in concentrations of serum β-carotene and bilirubin in old goats compared to goat kids (P < .05). In contrast, serum catalase activity, arylesterase, ceruloplasmin and uric acid values did not differ between the groups. The results obtained on the biochemical profiles and oxidant-antioxidant activities of Damascus goats will contribute to monitoring this breed's age-related health and nutritional status and establishing reference values.

Kaynakça

  • 1. Gül S, Biçer O. Fattening performance and carcass characteristics of yearling Damascus male goat. J Agric Food Environ Anim Sci. 2021;2(2):116-133
  • 2. Antunović Z, Novaković K, Klir Z, et al. Blood metabolic profile and acid-base status of Istrian goats-a critically endangered Croatian goat-in relation to age. Vet Arhiv. 2020;90(1):27-38.
  • 3. Kandlur A, Satyamoorthy K, Gangadharan G. Oxidative stress in cognitive and epigenetic aging: a retrospective glance. Front Mol Neurosci. 2020;18(13):41.
  • 4. Demirci-Çekiç S, Özkan G, Avan AN, Uzunboy S, Çapanoğlu E, Apak R. Biomarkers of oxidative stress and antioxidant defense. J Pharm Biomed Anal. 2022;209(5):114477.
  • 5. Cartee GD. Influence of age on skeletal muscle glucose transport and glycogen metabolism. Med Sci Sports Exerc. 1994;26(5):577-585.
  • 6. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activatedoxygen toxicity in the blood. Am J Obstet Gynecol. 1979;135(3):372-376.
  • 7. Ali SS, Ahsan H, Zia MK, Siddiqui T, Khan FH. Understanding oxidants and antioxidants: Classical team with new players. J Food Biochem. 2020;44(3): 13145.
  • 8. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-1111.
  • 9. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37(4):277-285.
  • 10. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882-888.
  • 11. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-169.
  • 12. Aebi H. Catalase in vitro methods in Enzymology. New York, ABD Academic press; 1984.
  • 13. Suzuki JP, Katoh NA. A simple and cheap method for measuring serum vitamin A in cattle using only spectrophotometer. Jpn J Vet Sci. 1990;52(6):1281-1283.
  • 14. Sunderman FW, Nomoto S. Measurement of human serum ceruloplasmin by its p-phenylene-diamine oxidase activity. Clin Chem. 1970;16(11):903-910.
  • 15. Bozukluhan K, Merhan O, Gökçe Hİ, et al. Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected Sheeppox virus. Kafkas Univ Vet Fak Derg. 2018;24(3):437-444.
  • 16. Salar S, Baştan İ, Baştan A, Pekcan M, Sel T. Investigation of changes in metabolic parameters and paraoxonase-1 during the transition period in Turkish Saanen goats. Kafkas Univ Vet Fak Derg. 2018;24(1):117-122.
  • 17. Lashari MH, Ahmad I, Masood S, et al. Hematological and biochemical parameters of pregnant and lactating goats in Rangeland of Cholistan Desert, Bahawalpur, Pakistan. J Hellenic Vet Med Soc. 2021;72(1):2781-2786.
  • 18. Karaşahin T, Aksoy NH, Haydardedeoglu AE, et al. Serum cholesterol levels in Hair goats of Aksaray Region. Indian J Anim Res. 2019;53(1):63-66.
  • 19. Carlos MML, Leite JHGM, Chaves DF, et al. Blood parameters in the Morada Nova sheep: influence of age, sex and body condition score. J Anim Plant Sci. 2015;25(4):950-955.
  • 20. Kiran S, Bhutta AM, Khan BA, Durrani S, Ali M, Iqbal F. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan. Asian Pac J Trop Biomed. 2012;2(4):304-306.
  • 21. Mondal M, Prakash BS. Changes in plasma non‐esterified fatty acids, glucose and α‐amino nitrogen and their relationship with body weight and plasma growth hormone in growing buffaloes (Bubalus bubalis). J Anim Physiol Anim Nutr (Berl). 2004;88(6):223-228.
  • 22. Al‐Rukibat R, Ismail Z, Al‐Zghoul MB, Hananeh W. Establishment of reference intervals of selected blood biochemical parameters in Shami goats. Vet Clin Pathol. 2020;49(4):665-668.
  • 23. Piccione G, Casella S, Lutri L, Vazzana I, Ferrantelli F, Caola G. Reference values for some haematological, haematochemical, and electrophoretic parameters in the Girgentana goat. Turk J Vet Anim Sci. 2010;34(2):197-204.
  • 24. Njidda AA, Hassan IT, Olatunji EA. Haematological and biochemical parameters of goat of semi arid environment fed on natural grazing rangeland of northern Nigeria. J Agr Vet Sci. 2013;3(2):1-8.
  • 25. Gurgoze SY, Icen H. The influence of age on clinical biochemical parameters in pure-bred Arabian mares. Equine Vet J. 2010;30(10):569-574.
  • 26. Yokus B, Cakir DU, Kanay Z, Gulten T, Uysal E. Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. J Vet Med A Physiol Pathol Clin Med. 2006;53(6):271-276.
  • 27. Devrim AK, Elmaz Ö, Mamak N, Sudağıdan M. Alterations in some clinical biochemistry values of Honamlı and Native Hair goats during pubertal development. Vet Arhiv. 2015;85(6):647-656.
  • 28. Abdolvahabi S, Zaeemi M, Mohri M, Naserian AA. Age related changes in serum biochemical profile of Saanen goat kids during the first three months of life. Revue Méd Vét. 2016;167(3):106-112.
  • 29. Celi P. The role of oxidative stress in small ruminants' health and production. R Bras Zootec. 2010;39(Suppl):348-363.
  • 30. Yatoo MI, Kanwar MS, Wani SA, Dinesh K, Umesh D. Alteration of metabolic biomarkers and oxidative stress indices in pashmina (Changthangi) goats under climate change. Indian J Anim Sci. 2016;86(4):405-413.
  • 31. Miller JK, Brzezinska-Slebodzinska E, Madsen FC. Oxidative stress, antioxidants, and animal function. J Dairy Sci. 1993;76(9):2812-2823.
  • 32. Gaál T, Speake B, Mezes M, Noble R, Surai P et al. Antioxidant parameters and ageing in some animal species. Comp Haematol Int. 1996;6(4):208-213.
  • 33. Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med. 2013;65:244-253.
  • 34. Simsek O, Ekici H, Cinar M, Atmaca N, Arıkan S, Guner B. Heavy metal accumulation and oxidative-antioxidative status in angora goats depending on age. Fresenius Environ Bull. 2015;24(8):2659-2663.
  • 35. Tekeli H, Kıral F, Bildik A, Yılmaz M, Kaçamaklı Z. Effect of aging on enzymatic and non-enzymatic antioxidant status in Saanen goats. Vet Med Zoot. 2015;71(93):67-71.
  • 36. Stahl W, Sies H. β-Carotene and other carotenoids in protection from sunlight. Am J Clin Nutr. 2012;96(5):1179-1184.
  • 37. Vítek L. Bilirubin as a signaling molecule. Med Res Rev. 2020;40(4):1335-1351.
  • 38. Mohri M, Sharifi K, Eidi S. Hematology and serum biochemistry of Holstein dairy calves: age-related changes and comparison with blood composition in adults. Res Vet Sci. 2007;83(1):30-39.
  • 39. Gavare V, Kiran K, Kiran D, Sachin S. Ceruloplasmin its role and significance: a review. IJBR. 2010;1(4):153-162.
  • 40. Kartal YK, Kurt S, Ekşi F, Salar S, Kısmalı G et al. Alteration of paraoxonase, ceruloplasmin and immunoglobulin G levels in hair goats at different ages. J Adv VetBio Sci Tech. 2022;7(1):8-13.
  • 41. Sautin YY, Johnson RJ. Uric acid: the oxidant-antioxidant paradox. Nucleos Nucleot Nucl. 2008;27(6-7):608-619.
  • 42. Toker A, Kadı M, Yıldırım AK, Aksoy H, Akçay F. Serum lipid profile paraoxonase and arylesterase activities in psoriasis. Cell Biochem Funct. 2009;27(3):176-180.
  • 43. Taha N, El-Noeman S, Korshom M, Mandour A, El-Feky M. Serum glutathione peroxidase and paraoxonase-1 enzyme activities in camel (Camelus dromedarius): effect of age and sex differences. Camel Pract Res. 2010;17(1):1-8.
Toplam 43 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Biyokimya, Veteriner Farmakoloji
Bölüm Araştırma Makaleleri
Yazarlar

Hakan Tekeli

Süleyman Şensoy

Gamze Sevri Ekren Aşıcı

Yayımlanma Tarihi 26 Nisan 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 19 Sayı: 1

Kaynak Göster

APA Tekeli, H., Şensoy, S., & Ekren Aşıcı, G. S. (2024). Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages. Veterinary Sciences and Practices, 19(1), 1-8. https://doi.org/10.17094/vetsci.1471422
AMA Tekeli H, Şensoy S, Ekren Aşıcı GS. Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages. Veterinary Sciences and Practices. Nisan 2024;19(1):1-8. doi:10.17094/vetsci.1471422
Chicago Tekeli, Hakan, Süleyman Şensoy, ve Gamze Sevri Ekren Aşıcı. “Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages”. Veterinary Sciences and Practices 19, sy. 1 (Nisan 2024): 1-8. https://doi.org/10.17094/vetsci.1471422.
EndNote Tekeli H, Şensoy S, Ekren Aşıcı GS (01 Nisan 2024) Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages. Veterinary Sciences and Practices 19 1 1–8.
IEEE H. Tekeli, S. Şensoy, ve G. S. Ekren Aşıcı, “Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages”, Veterinary Sciences and Practices, c. 19, sy. 1, ss. 1–8, 2024, doi: 10.17094/vetsci.1471422.
ISNAD Tekeli, Hakan vd. “Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages”. Veterinary Sciences and Practices 19/1 (Nisan 2024), 1-8. https://doi.org/10.17094/vetsci.1471422.
JAMA Tekeli H, Şensoy S, Ekren Aşıcı GS. Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages. Veterinary Sciences and Practices. 2024;19:1–8.
MLA Tekeli, Hakan vd. “Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages”. Veterinary Sciences and Practices, c. 19, sy. 1, 2024, ss. 1-8, doi:10.17094/vetsci.1471422.
Vancouver Tekeli H, Şensoy S, Ekren Aşıcı GS. Determination of Serum Biochemical Profile and Oxidant-Antioxidant Activities in Damascus Goats at Different Ages. Veterinary Sciences and Practices. 2024;19(1):1-8.

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