Immunohistochemical expression of anti-oxidants in bovine oviduct epithelial cells of estral and luteal phases
Abstract
The present study aimed to evaluate immunohistochemical distributions of anti-oxidative enzymes Cu ZnSuperoxide dismutase (SOD-1), catalase, and Glutation peroxidase-1 (GPX1) in the bovine oviduct epithelial cells of estral and luteal phases. The results indicated both ciliated and secretoric cells of the oviduct mucosa exibited varying degrees of immureactivity for all. The SOD-1 and GPX1 immunostainings were more conspicuous in luteal phase while catalase immunostaining was more apparent in estral phase, especially in the isthmus region of the oviduct. In contrast to catalase, GPX1 immunoractivity was absent or limited in the isthmus. All regions of the oviduct mucosa had similar SOD-1 immunoreactivity. SOD-1 and GPX1 immunoreactivities were more apparent in samples of the luteal phase while catalase immnureactivity was higher in those of the estral phase. Presence of anti-oxidative enzymes catalase, SOD, and GPX1 immunostainings in the bovine oviduct suggests that the bovine oviduct epithelial cells are most likely engaged into synthesis of such enzymes and possibly the source of anti-oxidative enzymes in oviduct fluid. The oviduct regions, each of which executes different reproductive functions, varied by means of catalase and GPX1 expressions, suggeting that anti-oxidants may possibly contribute to different physiological proceses in the reproductive cycle. Furthermore, anti-oxidant expressions also varied between luteal and estral phases, suggesting that oviduct epithelial cells are possibly influenced by hormonal changes in regard to anti-oxidant expression. Presence of SOD-1 immunoreactivity in some but not all basal cells of the oviduct epithelial lining should be further investigated for possible heterogeneities among basal cells and for origin of secretory and ciliated cells
References
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- 2. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S (2012): The effects of oxidative stress on female reproduction: a review Reprod Biol Endocrinol, 10,49-70.
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- 4. Aughey E, Frye FL (2001): Female reproductive system. 188 In: Comparative Veterinary Histology. Manson Publishing London pp.
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- 6. Celi P (2010): The role of oxidative stress in small ruminants’ health and production. R Bras Zootec, 39, 348- 363.
- 7. Chandra A, Surti N, Kesavan S, Agarwal A (2009): Significance of oxidative stress in human reproduction. Arch Med Sci 1A, 28-32.
- 8. Erdost H (2010): Dişi Genital Sistem. 232 In: Özer A (Ed), Veteriner Özel Histoloji Nobel Yayın Dağıtım, Ankara.
- 9. Guerin P, Mouatassim SEl, Menezo Y (2001): Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update, 7, 175-189.
- 10. Gupta S, Ghulmiyyah J, Sharma R, Halabi J, Agarwal A (2014): Power of proteomics in linking oxidative stress and female infertility. BioMed Research International http://dx.doi.org/10.1155/2014/916212.
- 11. Holt WV, Fazeli A (2010): The oviduct as a complex mediator of mammalian sperm function and selection. Mol Reprod Dev, 77, 934-943.
- 12. Hsu S M, Raine L, Fanger H (1981): Use of AvidinBiotin-Peroxidase Complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem, 29, 577-580.
- 13. Koppers AJ, De Iuliis GN, Finnie JM, McLaughlin EA, Aitken RJ (2008): Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab, 93, 3199- 207.
- 14. Lapointe J, Kimmins S, Leslie AM, Bilodeau JF (2005): Estrogen Selectively Up-Regulates the Phospholipid Hydroperoxide Glutathione Peroxidase in the Oviducts. Endocrinology, 146, 2583-2592.
- 15. Lapointe J, Bilodeau JF (2003): Antioxidant Defenses Are Modulated in the Cow Oviduct During the Estrous Cycle. Biol Reprod, 68, 1157-1164.
- 16. Lapointe S, Sullivan R, Sirard MA (1998): Binding of a bovine oviductal fluid catalase to mammalian spermatozoa. Biol Reprod, 58, 747-753.
- 17. Levebvre R, Chenoweth PJ, Drost M, LeClear CT, MacCubbin M, Dutton JT, Suarez SS (1995): Characterization of the Oviductal Sperm Reservoir in Cattle. Biol Reprod, 53, 1066-1074.
- 18. Maritim AC, Sanders RA, Watkins III JB (2003): Diabetes, oxidative stress, and antioxidants: a review. J Biochem Molecular Toxicology, 17, 24-38.
- 19. Mates JM (2000): Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology, 153, 83-104.
- 20. McNutt-Scott TL, Harris C (1998): Modulation of intracellular glutathione and cysteine metabolism in bovine oviduct epithelial cells cultured in vitro. Biol Reprod, 59, 314-20.
- 21. O’Flaherty CM, Beorlegui NB, Beconi MT (1999): Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction. Theriogenology, 52, 289-301.
- 22. Paik DY, Janzen DM, Schafenacker AM, Velasco VS, Shung MS, Cheng D, Huang J, Witte ON, Memarzadeh S (2012): Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: A site for injury and serous cancer initiation. Stem Cells, 30, 2487-2497.
- 23. Peters WM (1986): Nature of “basal” and “reserve”cells in oviductal and cervical epithelium in man. J Clin Pathol, 39, 306-312.
- 24. Priedkalns J, Leiser R (2006): Female Reproductive System. 262-265 In: Jo Ann Eurell (ed), Dellman’s Textbook of Veterinary Histology. Blackwell Publishing, Iowa.
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- 26. Rodriguez-Martinez H (2007): Role of the oviduct in sperm capacitation. Theriogenology, 68S, 138-146.
- 27. Rosenberg G, Dirksen G, Gründer HD, Grunert E, Krause D, Stöber M (1979). Femal genital system. In: Clinical Examination of Cattle. Ed: G. Rosenberg.Verlag Paul Parey, Berlin and Hamburg, p.: 329.
- 28. Rosenfeld CH., Schatten H (2007): Overview of Female Reproductive Organs. 103-104 In: Schatten H, Constantinescu GM. (ed), Comparative Reproductive Biology. Blackwell Publishing, Iowa.
- 29. Ross MH, Pawlina W (2011): Female Reproductive System. In: Histology A Text and Atlas. Lippincott Williams &Wikins, pp.845-848.
- 30. Roy M, Gauvreau D, Bilodeau JF (2008): Expression of superoxide dismutases in the bovine oviduct during the estrous cycle. Theriogenology, 70, 836-842.
- 31. Samuelson DA (2007): Female Reproductive System. 457- 459 In: Textbook of Veterinary Histology. Saunders Elsevier, Florida.
- 32. Shu S, Ju G, Fan L (1998): The glucose oxidase-dabnickel in peroxidase histochemistry of the nervous system. Neuroscience Lett, 85, 169-171.
- 33. Shull S, Heintz NH, Periasamy M, Manohar M, Janssen YMW, Marsh JP, Mossmann BT (1991): Differential regulation of antioxidant enzymes in response to oxidants. J Biol Chem, 266, 24398-24403.
- 34. Spooner R, Yilmaz O (2011): The Role of ReactiveOxygen-Species in Microbial Persistence and Inflammation. Int J Mol Sci, 12, 334-352.
- 35. Suarez SS, Brockman K, Lefebvre R (1997): Distribution of Mucus and Sperm in Bovine Oviducts after Artificial Insemination: The Physical Environment of the Oviductal Sperm Reservoir. Biol Reprod, 56, 447-453.
- 36. Uttara B, Singh AV, Zamboni P, Mahajan RT (2009): Oxidative Stress and Neurodegenerative Diseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options. Current Neuropharmacology, 7, 65-74.
- 37. Vandaele L, Thys M, Bijttebier J, Langendonckt AV, Donnay I, Maes D, Meyer E, Soom AV (2010): Shortterm exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development. Society for Reproduction and Fertility, DOI: 10.1530/REP-09- 0430.
Östral ve luteal dönemde sığır ovidukt epitelinde antioksidanların immunohistokimyasal ifadesi
Abstract
Sunulan çalışmada östral ve luteal dönemdeki sığır ovidukt epitelinde, anti-oksidatif enzimler Cu Zn-Süperoksit dismutaz (SOD-1), katalaz, ve Glutaston peroksidaz-1 (GPX1) immünohistokimyasal dağılımının incelenmesi amaçlanmıştır. Immunoperoksidaz test sonuçları ovidukt mukozasında silyalı ve sekretorik hücrelerin katalaz, SOD-1, and GPX1 için değişen derecelerde immunoreaktivite göstermiştir. SOD-1 ve GPX1 immünoreaktiviteleri luteal dönemde daha belirgin iken katalaz östral dönemde özellikle istmusta daha belirgin reaksiyon göstermiştir. Oviduktun tüm bölgeleri benzer SOD-1 immunoreaktivitisi göstermiştir. SOD-1 ve GPX1 luteal fazın örneklerinde, katalaz ise östral dönemin örneklerinde daha belirgin immunoreaktivite göstermiştir. Sığır oviduktunda katalaz, SOD-1, and GPX1 anti-oksidatif enzimlerinin immünoreaktivitesinin yer alması ovidukt epitel hücrelerinin bu enzimleri sentezlediğini ve ovidukt sıvısındaki anti-oksidant enzimlerin kaynağı olabileceğini düşündürmektedir. Farklı reprodüktif fonksiyonları yerine getiren ovidukt bölümleri katalaz ve GPX1 immunoreaktivitesi açısından farklılık göstermektedir. Bu durum anti-oksidanların seksüel siklüsta farklı fizyolojik süreçlere katılma olasılıklarını düşündürmektedir. Ayrıca luteal ve östral dönem arasında anti-oksidanların göstermiş olduğu farklı immunreaksiyonun ovidukt epitel hücrelerinin üreme hormonlarından anti-oksidant ekspresyonu açısından etkilendiğini düşündürmektedir. SOD-1 immünoreaktivitesinin ovidukt epitelindeki bazal hücrelerin bazılarında görülüp bazılarında görülmemesi, bu hücrelerdeki gerek heterojenite gerekse silyalı ve sekretorik hücrelerin kökenleri açısından incelenmesi gerekmektedir
Keywords
References
- 1. Abughrien BM, Dore MAP, McGeady TA, Fitzpatrick E (2000): Intraepithelial Leucocytes in the bovine uterine tube. Cells Tissues Organs, 166, 20-30.
- 2. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S (2012): The effects of oxidative stress on female reproduction: a review Reprod Biol Endocrinol, 10,49-70.
- 3. Agarwal A, Gupta S, Sharma RK (2005): Role of oxidative stress in female reproduction. Reprod Biol Endocrinol, 3, 28.
- 4. Aughey E, Frye FL (2001): Female reproductive system. 188 In: Comparative Veterinary Histology. Manson Publishing London pp.
- 5. Bhatt P, Kadam K, Saxena A, Natraj U (2004): Fertilization, embryonic development and oviductal environment: role of estrogen induced oviductal glycoprotein. Indian J Exp Biol, 42, 1043-1055.
- 6. Celi P (2010): The role of oxidative stress in small ruminants’ health and production. R Bras Zootec, 39, 348- 363.
- 7. Chandra A, Surti N, Kesavan S, Agarwal A (2009): Significance of oxidative stress in human reproduction. Arch Med Sci 1A, 28-32.
- 8. Erdost H (2010): Dişi Genital Sistem. 232 In: Özer A (Ed), Veteriner Özel Histoloji Nobel Yayın Dağıtım, Ankara.
- 9. Guerin P, Mouatassim SEl, Menezo Y (2001): Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update, 7, 175-189.
- 10. Gupta S, Ghulmiyyah J, Sharma R, Halabi J, Agarwal A (2014): Power of proteomics in linking oxidative stress and female infertility. BioMed Research International http://dx.doi.org/10.1155/2014/916212.
- 11. Holt WV, Fazeli A (2010): The oviduct as a complex mediator of mammalian sperm function and selection. Mol Reprod Dev, 77, 934-943.
- 12. Hsu S M, Raine L, Fanger H (1981): Use of AvidinBiotin-Peroxidase Complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem, 29, 577-580.
- 13. Koppers AJ, De Iuliis GN, Finnie JM, McLaughlin EA, Aitken RJ (2008): Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab, 93, 3199- 207.
- 14. Lapointe J, Kimmins S, Leslie AM, Bilodeau JF (2005): Estrogen Selectively Up-Regulates the Phospholipid Hydroperoxide Glutathione Peroxidase in the Oviducts. Endocrinology, 146, 2583-2592.
- 15. Lapointe J, Bilodeau JF (2003): Antioxidant Defenses Are Modulated in the Cow Oviduct During the Estrous Cycle. Biol Reprod, 68, 1157-1164.
- 16. Lapointe S, Sullivan R, Sirard MA (1998): Binding of a bovine oviductal fluid catalase to mammalian spermatozoa. Biol Reprod, 58, 747-753.
- 17. Levebvre R, Chenoweth PJ, Drost M, LeClear CT, MacCubbin M, Dutton JT, Suarez SS (1995): Characterization of the Oviductal Sperm Reservoir in Cattle. Biol Reprod, 53, 1066-1074.
- 18. Maritim AC, Sanders RA, Watkins III JB (2003): Diabetes, oxidative stress, and antioxidants: a review. J Biochem Molecular Toxicology, 17, 24-38.
- 19. Mates JM (2000): Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology, 153, 83-104.
- 20. McNutt-Scott TL, Harris C (1998): Modulation of intracellular glutathione and cysteine metabolism in bovine oviduct epithelial cells cultured in vitro. Biol Reprod, 59, 314-20.
- 21. O’Flaherty CM, Beorlegui NB, Beconi MT (1999): Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction. Theriogenology, 52, 289-301.
- 22. Paik DY, Janzen DM, Schafenacker AM, Velasco VS, Shung MS, Cheng D, Huang J, Witte ON, Memarzadeh S (2012): Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: A site for injury and serous cancer initiation. Stem Cells, 30, 2487-2497.
- 23. Peters WM (1986): Nature of “basal” and “reserve”cells in oviductal and cervical epithelium in man. J Clin Pathol, 39, 306-312.
- 24. Priedkalns J, Leiser R (2006): Female Reproductive System. 262-265 In: Jo Ann Eurell (ed), Dellman’s Textbook of Veterinary Histology. Blackwell Publishing, Iowa.
- 25. Riley JC, Behrman HR (1991): Oxygen radicals and reactive oxygen species in reproduction., 198, 781-91.
- 26. Rodriguez-Martinez H (2007): Role of the oviduct in sperm capacitation. Theriogenology, 68S, 138-146.
- 27. Rosenberg G, Dirksen G, Gründer HD, Grunert E, Krause D, Stöber M (1979). Femal genital system. In: Clinical Examination of Cattle. Ed: G. Rosenberg.Verlag Paul Parey, Berlin and Hamburg, p.: 329.
- 28. Rosenfeld CH., Schatten H (2007): Overview of Female Reproductive Organs. 103-104 In: Schatten H, Constantinescu GM. (ed), Comparative Reproductive Biology. Blackwell Publishing, Iowa.
- 29. Ross MH, Pawlina W (2011): Female Reproductive System. In: Histology A Text and Atlas. Lippincott Williams &Wikins, pp.845-848.
- 30. Roy M, Gauvreau D, Bilodeau JF (2008): Expression of superoxide dismutases in the bovine oviduct during the estrous cycle. Theriogenology, 70, 836-842.
- 31. Samuelson DA (2007): Female Reproductive System. 457- 459 In: Textbook of Veterinary Histology. Saunders Elsevier, Florida.
- 32. Shu S, Ju G, Fan L (1998): The glucose oxidase-dabnickel in peroxidase histochemistry of the nervous system. Neuroscience Lett, 85, 169-171.
- 33. Shull S, Heintz NH, Periasamy M, Manohar M, Janssen YMW, Marsh JP, Mossmann BT (1991): Differential regulation of antioxidant enzymes in response to oxidants. J Biol Chem, 266, 24398-24403.
- 34. Spooner R, Yilmaz O (2011): The Role of ReactiveOxygen-Species in Microbial Persistence and Inflammation. Int J Mol Sci, 12, 334-352.
- 35. Suarez SS, Brockman K, Lefebvre R (1997): Distribution of Mucus and Sperm in Bovine Oviducts after Artificial Insemination: The Physical Environment of the Oviductal Sperm Reservoir. Biol Reprod, 56, 447-453.
- 36. Uttara B, Singh AV, Zamboni P, Mahajan RT (2009): Oxidative Stress and Neurodegenerative Diseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options. Current Neuropharmacology, 7, 65-74.
- 37. Vandaele L, Thys M, Bijttebier J, Langendonckt AV, Donnay I, Maes D, Meyer E, Soom AV (2010): Shortterm exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development. Society for Reproduction and Fertility, DOI: 10.1530/REP-09- 0430.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA95HN29DT |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2016 |
| Published in Issue | Year 2016 Volume: 63 Issue: 2 |