Safkan Bir Atın Lokal Polyester Reçine ile Kesitli Böbrek Plastinasyonunun Anatomik Değerlendirmesi ve Hazırlama Prosedürü
Abstract
Bu çalışma, lokal polyester reçine ile böbreğin ince kesit plastinasyonu için bir protokol geliştirmeyi ve örneğin anatomik detaylarını incelemeyi amaçlamıştır. Örnek %10 formalin fiksasyon solüsyonu ile sabitlendi ve ardından 3 mm'lik dilimlere ayrıldı. Polyester plastinasyon tekniğinin dört aşaması kullanıldı. İlk olarak örnekler dehidrasyon için -25ºC'de %99,5 aseton banyosunda tutulmuştur. Bu işlemden sonra kesitler polyester reçineye yerleştirildi ve vakum altında oda sıcaklığında (20ºC) impregnasyon işlemine başlandı. Zorlu impregnasyon işleminden sonra kürleme odaları oluşturuldu ve kürleme işlemine ultraviyole ışık altında devam edildi. Uygulanan her aşamanın verileri dikkatlice kaydedildi ve böbrek bölümlerinin polyester plastinasyonu için protokol başarıyla oluşturuldu. Örnekler, ince bir kesit görünümü sayesinde farklı bir bakış açısıyla gözlemlenmiştir. Örneklerin anatomik morfolojisi ve böbreğin kesit bölümlerine ait yapılar, böbrek paraşimi ve dolaşım bileşenleri gibi, iyi korunmuştur. Elde edilen son ürünler, böbreklerin kesitsel anatomik çalışmaları için iyi birer eğitim materyalleri olarak kullanılabileceği düşünülmektedir.
Project Number
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References
- Baptista CA, DeJong K, Latorre R, et al (2019): P40 polyester sheet plastination technique for brain and body slices: The vertical and horizontal flat chamber methods. Anat Hist Embryol, 48, 572-576.
- Chisholm F, Varsou O (2018): Resin-embedded anatomical cross-sections as a teaching adjunct for medical curricula: is this technique an alternative to potting and plastination? J Anat, 233, 98-105.
- Entius CAS, Rijn VRR, Zwamborn AW, et al (2004): Influence of Formaldehyde/Phenol fixation on MRI of the stifle joint and correlation with plastinated slices. J Int Soc Plastination, 19, 26-32.
- Gao H, Liu J, Yu S, et al (2006): A new polyester technique for sheet plastination. J Int Soc Plastination, 21, 7-10.
- Guerrero M, Vargas C, Alarcón E, et al (2019): Desarrollo de un protocolo de plastinación de cortes con resina poliéster aplicado a secciones de cerebro humano. Int J Morphol, 37, 1557-1563.
- Henry RW, Latorre R (2007): Polyester plastination of biological tissue: P40 Technique for Brain Slices. J Int Soc Plastination, 22, 59-68.
- Konig HE, Maierl J, Liebich HG (2020): Veterinary anatomy of domestic mammals, textbook and colour atlas, 7th edition. Thieme.
- Kürtül I, Hammer N, Rabi S, et al (2012): Oblique sectional planes of block plastinates eased by Sac Plastination. Ann Anat, 194, 404–406.
- Latorre R, Arencibia A, Gil F, et al (2003): P-40 and S10 plastinated slices: An aid to interpreting MR images of the equine tarsus. J Int Soc Plastination, 18, 14-22.
- Latorre R, Henry RW (2007): Polyester plastination of biological tissue: P40 technique for body slices. J Int Soc Plastination, 22, 69-77.
- Nickel R, Schummer A, Seiferle E, et al (1979): The viscera of the domestic mammals, Springer-Verlag.
- Nomina Anatomica Veterinaria (2017): International Committee on Veterinary Gross Anatomical Nomenclature and authorized by the general assembly of the World Association of Veterinary Anatomist, 6th ed. Editorial Committee Hanover (Germany).
- Ogaili R, Baker SSM, Sui HJ (2018): Using of polyester P45 plastinated sheet specimens in teaching anatomy, pathology and radiology courses. Int J Chemtech Res, 11, 393-398.
- Ottone NE, Guerrero M, Alarcón E, et al (2020): Statistical analysis of shrinkage levels of human brain slices preserved by sheet plastination technique with polyester resin. Int J Morphol, 38, 13-16.
- Pashaei S (2010): A brief review on the history, methods, and applications of plastination. Int J Morphol, 28, 1075-1079.
- Pendovski L, Petkov V, Popovska-Percinic F, et al (2008): Silicone plastination procedure for producing thin, semitransparent tissue slices: A Study using the Pig. J Int Soc Plastination, 23, 10-16.
Anatomical evaluation and preparation procedure of a cross-sectioned kidney plastination of a thoroughbred horse with local polyester resin
Abstract
This study aimed to develop a protocol for the thin section plastination of the kidney with local polyester resin and examine the anatomical details of this specimen. The sample was fixed with 10% formalin fixation solution and then sectioned into 3 mm slices. The four stages of the polyester plastination technique were used. Firstly, the samples were kept in 99.5% acetone baths at -25ºC for the dehydration. After this process, the sections were placed in polyester resin and the impregnation process was started at room temperature (20ºC) under vacuum. Following the forced impregnation, the curing chambers were constructed and the curing process was continued under ultraviolet light. The data of each applied stage were recorded carefully and the protocol for polyester plastination of kidney sections was successfully established. The specimens were observed from a different point of view with a thin cross-sectional appearance. The anatomical morphology and the structures of the sections of the kidney such as renal parenchyma and circulatory components were preserved well. The final products could be used as educational samples for cross-sectional anatomical training of kidneys.
Ethical Statement
The present study was approved by the Ankara University Animal Experiments Local Ethics Committee (Decision No. 2021-21-189, Ankara, Türkiye).
Project Number
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Thanks
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References
- Baptista CA, DeJong K, Latorre R, et al (2019): P40 polyester sheet plastination technique for brain and body slices: The vertical and horizontal flat chamber methods. Anat Hist Embryol, 48, 572-576.
- Chisholm F, Varsou O (2018): Resin-embedded anatomical cross-sections as a teaching adjunct for medical curricula: is this technique an alternative to potting and plastination? J Anat, 233, 98-105.
- Entius CAS, Rijn VRR, Zwamborn AW, et al (2004): Influence of Formaldehyde/Phenol fixation on MRI of the stifle joint and correlation with plastinated slices. J Int Soc Plastination, 19, 26-32.
- Gao H, Liu J, Yu S, et al (2006): A new polyester technique for sheet plastination. J Int Soc Plastination, 21, 7-10.
- Guerrero M, Vargas C, Alarcón E, et al (2019): Desarrollo de un protocolo de plastinación de cortes con resina poliéster aplicado a secciones de cerebro humano. Int J Morphol, 37, 1557-1563.
- Henry RW, Latorre R (2007): Polyester plastination of biological tissue: P40 Technique for Brain Slices. J Int Soc Plastination, 22, 59-68.
- Konig HE, Maierl J, Liebich HG (2020): Veterinary anatomy of domestic mammals, textbook and colour atlas, 7th edition. Thieme.
- Kürtül I, Hammer N, Rabi S, et al (2012): Oblique sectional planes of block plastinates eased by Sac Plastination. Ann Anat, 194, 404–406.
- Latorre R, Arencibia A, Gil F, et al (2003): P-40 and S10 plastinated slices: An aid to interpreting MR images of the equine tarsus. J Int Soc Plastination, 18, 14-22.
- Latorre R, Henry RW (2007): Polyester plastination of biological tissue: P40 technique for body slices. J Int Soc Plastination, 22, 69-77.
- Nickel R, Schummer A, Seiferle E, et al (1979): The viscera of the domestic mammals, Springer-Verlag.
- Nomina Anatomica Veterinaria (2017): International Committee on Veterinary Gross Anatomical Nomenclature and authorized by the general assembly of the World Association of Veterinary Anatomist, 6th ed. Editorial Committee Hanover (Germany).
- Ogaili R, Baker SSM, Sui HJ (2018): Using of polyester P45 plastinated sheet specimens in teaching anatomy, pathology and radiology courses. Int J Chemtech Res, 11, 393-398.
- Ottone NE, Guerrero M, Alarcón E, et al (2020): Statistical analysis of shrinkage levels of human brain slices preserved by sheet plastination technique with polyester resin. Int J Morphol, 38, 13-16.
- Pashaei S (2010): A brief review on the history, methods, and applications of plastination. Int J Morphol, 28, 1075-1079.
- Pendovski L, Petkov V, Popovska-Percinic F, et al (2008): Silicone plastination procedure for producing thin, semitransparent tissue slices: A Study using the Pig. J Int Soc Plastination, 23, 10-16.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Anatomy and Physiology |
| Journal Section | Research Article |
| Authors | |
| Project Number | - |
| Publication Date | September 29, 2023 |
| Published in Issue | Year 2023 Volume: 70 Issue: 4 |
