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Research Article
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Year 2025, Volume: 72 Issue: 3, 277 - 286, 01.07.2025

Sevil Atalay Vural , Rıfkı Hazıroğlu , Osman Kutsal , Gözde Yücel Tenekeci , Arda Selin Tunç , Yanad Abou Monsef , Ozan Ahlat , Kürşat Filikci , Özgür Özöner , Oya Burçin Demirtaş

https://doi.org/10.33988/auvfd.1526014

Abstract

Project Number

21A0239003

References

  • 1. Baghezza S, Mamache B, Bennoune O, et al (2021): Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique. Comp Clin Path, 30, 301–310.
  • 2. Benavides J, González L, Dagleish M, et al (2015): Diagnostic pathology in microbial diseases of sheep or goats. Vet Microbiol, 181, 15-26.
  • 3. Caswell J L, Williams KJ (2015): Respiratory system. In: Jubb, Kennedy, and Palmer’s pathology of domestic animals, Volume 2, 6th edn., Maxie MG (ed), Elsevier, St. Louis, Missouri. pp 465–591.
  • 4. Chakraborty S, Kumar A, Tiwari R, et al (2014): Advances in diagnosis of respiratory diseases of small ruminants. Vet Med Int, 2014, 508304.
  • 5. Chan S, Filézac de L’Etang A, Rangell L, et al (2018): A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples. Plos One, 13, e0207619.
  • 6. Çeribaşı AO, Ozkaraca M, Çeribaşı S, et al (2014): Histopathologic, immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Rev Med Vet, 165, 201-212.
  • 7. Durham PJ, Stevenson BJ, Farquharson BC (1979): Rotavirus and coronavirus associated diarrhoea in domestic animals. N Z Vet J, 27, 30–32.
  • 8. Heller MC, Chigerwe M (2018): Diagnosis and treatment of infectious enteritis in neonatal and juvenile ruminants. Veterinary Clinics: Food Animal Practice, 34, 101-117.
  • 9. Hindson JC, Winter A (2008): Manual of sheep diseases, 2nd edition. 90-102. John Wiley & Sons.
  • 10. Holmøy IH, Waage S, Granquist EG, et al (2017): Early neonatal lamb mortality: postmortem findings. Animal, 11, 295-305.
  • 11. Jarikre TA, Emikpe BO (2017): First report of immunohistochemical detection of Peste des petit ruminants, parainfluenza 3 and respiratory syncytial viral antigens in lungs of Nigerian goats. J Immunoassay Immunochem, 38, 555–568.
  • 12. Martella V, Decaro N, Buonavoglia C (2015): Enteric viral infections in lambs or kids. Vet Microbiol, 181, 154-160.
  • 13. Munday JS, Bentall H, Aberdein D, et al (2020): Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand. N Z Vet J, 68, 242–246.
  • 14. Ozmen O, Yukari BA, Haligur M, et al (2006): Observations and immunohistochemical detection of Coronavirus, Cryptosporidium parvum and Giardia intestinalis in neonatal diarrhoea in lambs and kids. Schweiz Arch Tierh, 148, 357–364.
  • 15. Ozmen O, Haligur M, Aydogan A, et al (2018): Immunohistochemical detection of viral etiopathogenesis in lambs and goat kids with neonatal diarrhea. Acta Sci Vet, 46, 8.
  • 16. Sheehan M, Cassidy JP, Brady J, et al (2007): An aetiopathological study of chronic bronchopneumonia in lambs in Ireland. Veterinary journal (London, England, 1997), 173, 630–637.
  • 17. Terzi OS, Kara E, Şenel Y, et al (2022): Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea. Ank Univ Vet Fak Derg, 70, 81-86.
  • 18. Uzal FA, Plattner BL, Hostetter JM (2015): Alimentary system. 1–257. In: Maxie MG (Ed), Jubb, Kennedy, and Palmer’s pathology of domestic animals, Volume 2, 6th ed. Elsevier, St. Louis, Missouri.
  • 19. Yener Z, Sağlam YS, Timurkaan N, et al (2005): Immunohistochemical detection of parainfluenza type 3 virus antigens in paraffin sections of pneumonic caprine lungs. J Vet Med A Physiol Pathol Clin Med, 52, 268–271.

Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality

Year 2025, Volume: 72 Issue: 3, 277 - 286, 01.07.2025

Sevil Atalay Vural , Rıfkı Hazıroğlu , Osman Kutsal , Gözde Yücel Tenekeci , Arda Selin Tunç , Yanad Abou Monsef , Ozan Ahlat , Kürşat Filikci , Özgür Özöner , Oya Burçin Demirtaş

https://doi.org/10.33988/auvfd.1526014

Abstract

It was aimed to identify diseases using immunocytochemical, immunohistochemical, and in-situ hybridization methods, to determine sensitivity and specificity among these techniques, and to highlight their advantages and disadvantages for certain respiratory (Mycoplasma pneumonia, Pasteurella spp., Respiratory syncytial virus, Parainfluenza virus 3) and enteric (Coronavirus, Rotavirus, Escherichia coli, Clostridium spp.) agents. The obtained results were compared, and although the immunocytochemical method was found to be the fastest, immunohistochemistry was proved to be the most reliable method. Our other aim was to establish pathological diagnostic panels for neonatal infections. All antibodies tested were found to be positive except for Pasteurella multocida. The immunohistochemical findings of the study indicate that nearly all cases that result in death involved mixed infections.

Keywords

Immunocytochemistry Immunohistochemistry infection in situ hybridisation neonatal small ruminant

Ethical Statement

This study does not present any ethical concerns.

Project Number

21A0239003

References

  • 1. Baghezza S, Mamache B, Bennoune O, et al (2021): Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique. Comp Clin Path, 30, 301–310.
  • 2. Benavides J, González L, Dagleish M, et al (2015): Diagnostic pathology in microbial diseases of sheep or goats. Vet Microbiol, 181, 15-26.
  • 3. Caswell J L, Williams KJ (2015): Respiratory system. In: Jubb, Kennedy, and Palmer’s pathology of domestic animals, Volume 2, 6th edn., Maxie MG (ed), Elsevier, St. Louis, Missouri. pp 465–591.
  • 4. Chakraborty S, Kumar A, Tiwari R, et al (2014): Advances in diagnosis of respiratory diseases of small ruminants. Vet Med Int, 2014, 508304.
  • 5. Chan S, Filézac de L’Etang A, Rangell L, et al (2018): A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples. Plos One, 13, e0207619.
  • 6. Çeribaşı AO, Ozkaraca M, Çeribaşı S, et al (2014): Histopathologic, immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Rev Med Vet, 165, 201-212.
  • 7. Durham PJ, Stevenson BJ, Farquharson BC (1979): Rotavirus and coronavirus associated diarrhoea in domestic animals. N Z Vet J, 27, 30–32.
  • 8. Heller MC, Chigerwe M (2018): Diagnosis and treatment of infectious enteritis in neonatal and juvenile ruminants. Veterinary Clinics: Food Animal Practice, 34, 101-117.
  • 9. Hindson JC, Winter A (2008): Manual of sheep diseases, 2nd edition. 90-102. John Wiley & Sons.
  • 10. Holmøy IH, Waage S, Granquist EG, et al (2017): Early neonatal lamb mortality: postmortem findings. Animal, 11, 295-305.
  • 11. Jarikre TA, Emikpe BO (2017): First report of immunohistochemical detection of Peste des petit ruminants, parainfluenza 3 and respiratory syncytial viral antigens in lungs of Nigerian goats. J Immunoassay Immunochem, 38, 555–568.
  • 12. Martella V, Decaro N, Buonavoglia C (2015): Enteric viral infections in lambs or kids. Vet Microbiol, 181, 154-160.
  • 13. Munday JS, Bentall H, Aberdein D, et al (2020): Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand. N Z Vet J, 68, 242–246.
  • 14. Ozmen O, Yukari BA, Haligur M, et al (2006): Observations and immunohistochemical detection of Coronavirus, Cryptosporidium parvum and Giardia intestinalis in neonatal diarrhoea in lambs and kids. Schweiz Arch Tierh, 148, 357–364.
  • 15. Ozmen O, Haligur M, Aydogan A, et al (2018): Immunohistochemical detection of viral etiopathogenesis in lambs and goat kids with neonatal diarrhea. Acta Sci Vet, 46, 8.
  • 16. Sheehan M, Cassidy JP, Brady J, et al (2007): An aetiopathological study of chronic bronchopneumonia in lambs in Ireland. Veterinary journal (London, England, 1997), 173, 630–637.
  • 17. Terzi OS, Kara E, Şenel Y, et al (2022): Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea. Ank Univ Vet Fak Derg, 70, 81-86.
  • 18. Uzal FA, Plattner BL, Hostetter JM (2015): Alimentary system. 1–257. In: Maxie MG (Ed), Jubb, Kennedy, and Palmer’s pathology of domestic animals, Volume 2, 6th ed. Elsevier, St. Louis, Missouri.
  • 19. Yener Z, Sağlam YS, Timurkaan N, et al (2005): Immunohistochemical detection of parainfluenza type 3 virus antigens in paraffin sections of pneumonic caprine lungs. J Vet Med A Physiol Pathol Clin Med, 52, 268–271.
There are 19 citations in total.

Details

Primary Language English
Subjects Veterinary Pathology
Journal Section Research Article
Authors

Sevil Atalay Vural 0000-0003-2111-3381

Rıfkı Hazıroğlu 0000-0002-1134-3581

Osman Kutsal 0000-0003-3599-6867

Gözde Yücel Tenekeci 0000-0002-2586-8346

Arda Selin Tunç 0000-0002-4813-7626

Yanad Abou Monsef 0000-0002-4929-9395

Ozan Ahlat 0000-0002-2580-8140

Kürşat Filikci 0000-0001-9710-9480

Özgür Özöner 0000-0001-7354-0655

Oya Burçin Demirtaş 0000-0003-0850-2374

Project Number 21A0239003
Early Pub Date March 3, 2025
Publication Date July 1, 2025
Submission Date August 1, 2024
Acceptance Date February 17, 2025
Published in Issue Year 2025 Volume: 72 Issue: 3

Cite

APA Atalay Vural, S., Hazıroğlu, R., Kutsal, O., … Yücel Tenekeci, G. (2025). Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 72(3), 277-286. https://doi.org/10.33988/auvfd.1526014
AMA Atalay Vural S, Hazıroğlu R, Kutsal O, et al. Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality. Ankara Univ Vet Fak Derg. July 2025;72(3):277-286. doi:10.33988/auvfd.1526014
Chicago Atalay Vural, Sevil, Rıfkı Hazıroğlu, Osman Kutsal, Gözde Yücel Tenekeci, Arda Selin Tunç, Yanad Abou Monsef, Ozan Ahlat, Kürşat Filikci, Özgür Özöner, and Oya Burçin Demirtaş. “Comparative Study of Immunocytological, Immunohistochemical and In-Situ Hybridization Methods in Small Ruminant Neonatal Mortality”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 72, no. 3 (July 2025): 277-86. https://doi.org/10.33988/auvfd.1526014.
EndNote Atalay Vural S, Hazıroğlu R, Kutsal O, Yücel Tenekeci G, Tunç AS, Abou Monsef Y, Ahlat O, Filikci K, Özöner Ö, Demirtaş OB (July 1, 2025) Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality. Ankara Üniversitesi Veteriner Fakültesi Dergisi 72 3 277–286.
IEEE S. Atalay Vural et al., “Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality”, Ankara Univ Vet Fak Derg, vol. 72, no. 3, pp. 277–286, 2025, doi: 10.33988/auvfd.1526014.
ISNAD Atalay Vural, Sevil et al. “Comparative Study of Immunocytological, Immunohistochemical and In-Situ Hybridization Methods in Small Ruminant Neonatal Mortality”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 72/3 (July2025), 277-286. https://doi.org/10.33988/auvfd.1526014.
JAMA Atalay Vural S, Hazıroğlu R, Kutsal O, Yücel Tenekeci G, Tunç AS, Abou Monsef Y, Ahlat O, Filikci K, Özöner Ö, Demirtaş OB. Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality. Ankara Univ Vet Fak Derg. 2025;72:277–286.
MLA Atalay Vural, Sevil et al. “Comparative Study of Immunocytological, Immunohistochemical and In-Situ Hybridization Methods in Small Ruminant Neonatal Mortality”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 72, no. 3, 2025, pp. 277-86, doi:10.33988/auvfd.1526014.
Vancouver Atalay Vural S, Hazıroğlu R, Kutsal O, Yücel Tenekeci G, Tunç AS, Abou Monsef Y, et al. Comparative study of immunocytological, immunohistochemical and in-situ hybridization methods in small ruminant neonatal mortality. Ankara Univ Vet Fak Derg. 2025;72(3):277-86.
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