Research Article
Effects of Short-Term Transportation and Lairage Time on Meat Quality, CAST, CAPN1, CAPN3, HSPA1A Gene Expression Levels and Myofibrillary Structure of M. longissimus dorsi of Kıvırcık Breed Sheep
Abstract
The objective of this study was to examine the effect of short term transportation and lairage time on meat quality, myofibrillary structure and calpastatin (CAST), µ-calpain (CAPN1), calpain 3 (CAPN3) and heat shock protein 70 (HSPA1A) expression levels of longissimus dorsi of Kıvırcık breed sheep (n=40). Meat quality was evaluated by muscle glycogen amounts, pH, temperature, cooking loss, water holding capacity, shear force, instrumental color, sarcomere length and organoleptic properties. Cellular changes in the muscle during the ageing process was scanned by electron microscopy and CAST, CAPN1, CAPN3, HSPA1A expression levels were measured to reveal the association on meat tenderness. As a result, positive effect of long lairage time groups was observed in the pH, glycogen, SF values and organoleptic evaluation. CAST, CAPN1, CAPN3 expression showed no difference, however, HSPA1A showed significant difference in aging process. In conclusion, genotypic differences, their effect on gene expression and protein level on meat quality should be further investigated.
Keywords
CAST CAPN1 CAPN3 HSPA1A Lairage Time Meat Quality Myofibrillary Structure Sheep Transportation
Ethical Statement
This study was approved by the İstanbul University Animal Experiments Local Ethics Committee (2016/15).
Supporting Institution
This work was supported by TÜBİTAK (116O768) and the Research Fund of İstanbul-Cerrahpaşa University (25293).
Project Number
116O654 and 25293
References
- Altınçekiç ŞÖ, Koyuncu, M (2010): Nakil koşullarının hayvan refahı üzerine etkileri. Hay Üret, 51, 8.
- AMSA (1991): American Meat Association Guidelines for Meat Colour Evaluation. Nat. Live Stock and Meat Board Chicago, USA.
- Bagatoli A, Gasparino E, Soares MA, et al (2013): Expression of calpastatin and myostatin genes associated with lamb meat quality. Genet Mol Res, 12, 4, 6168-6175.
- Bendall JR (1973): The biochemistry of rigor mortis and cold contracture. In Proceedings of the 19th European Meeting of Meat Research Workers. Paris, France.
- Calvo JH, Iguacel LP, Kirinus JK, et al (2014): A new single nucleotide polymorphism in the calpastatin (CAST) gene associated with beef tenderness. Meat Sci, 96, 775-782.
- Catts VS, Catts SV, Fernandez HR, et al (2005): Microarray study of post-mortem mRNA degradation in mouse brain tissue. Brain Res Mol Brain Res, 138, 164-177.
- Cerdeño AI, Vieira C, Serrano E, et al (2006): Carcass and meat quality in Brown fattened young bulls: effect of rearing method and slaughter weight. J Anim Feed Sci, 15, 7.
- Chulayo AY, Bradley G, Muchenje V (2016): Effects of transport distance, lairage time and stunning efficiency on cortisol, glucose, HSPA1A and how they relate with meat quality in cattle. Meat Sci, 117, 89-96.
- Cross HR, West RL, Dutson TR (1981): Comparison of methods for measuring sarcomere length in beef semitendinosus muscle. Meat Sci, 5, 261-266.
- Dangi SS, Gupta M, Nagar V, et al (2014). Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats. Int J Biometeorol, 58, 2085-2093.
- Del Campo M, Brito G, Soares de Lima J, et al (2010): Temperament and lairage time effects on carcass and meat quality traits in steers. Meat Sci, 86, 908-914.
- Diaz MT, Vieira C, Perez C, et al (2014): Effect of lairage time ( 0h, 3 h, 6 h or 12 h) on glycogen content and meat quality parameters in suckling lambs. Meat Sci, 96, 653-660.
- Ekiz B, Ekiz EE, Kocak O, et al (2012): Effect of pre-slaughter management regarding transportation and time in lairage on certain stress parameters, carcass and meat quality characteristics in Kivircik lambs. Meat Sci, 90, 967-976.
- Ferguson DM, Warner RD (2008): Have we underestimated the impact of pre-slaughter stress on meat quality in ruminants? Meat Sci, 80, 12-19.
- Ferreira GB, Andrade CL, Costa F, et al (2006): Effects of transport time and rest period on the quality of electrically stimulated male cattle carcasses. Meat Sci, 74, 459-466.
- Ferreira PG, Munoz-Aguirre M, Reverter F, et al (2018): The effects of death and post-mortem cold ischemia on human tissue transcriptomes. Nat Commun, 9, 490.
- Fitzpatrick R, Casey OM, Morris D, et al (2002): Postmortem stability of RNA isolated from bovine reproductive tissues. Biochim Biophys Acta, 1574, 10-14.
- Gallo C, Lizondo G, Knowles TG (2003): Effects of journey and lairage time on steers transported to slaughter in Chile. Vet Rec, 152, 361-364.
- Gandolfi G, Pomponio L, Ertbjerg P, et al (2011): Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality. Meat Sci, 88, 694-700.
- Hannula T, Puolanne E (2004): The effect of cooling rate on beef tenderness: The significance of pH at 7 degrees C. Meat Sci, 67, 403-408.
- HNSRKY (2014): Hayvan Nakilleri Sırasında Refah ve Korunması Yönetmeliği. Available at https://www.resmigazete.gov.tr/eskiler/2014/02/20140205-3.htm Resmi Gazete. (Accessed August 17, 2022).
- Holman BWB, Collins D, Kilgannon AK, et al (2020): Using shear force, sarcomere length, particle size, collagen content, and protein solubility metrics to predict consumer acceptance of aged beef tenderness. J Texture Stud, 51, 559-566.
- ISO (1985): Sensory Analysis – Methodology – General Guidance. International Organization for Standardization, Paris.
- ISO (1988): Sensory Analysis – General Guidance for Design of Test Rooms. International Organization for Standardization, Paris.
- ISO (1993): Sensory Analysis – General Guidance for the Selection, Training and Monitoring of Assessors. Part 1: Selected Assessors. International Organization for Standardization, Genève.
- Ivanov NT (2020): Sheep welfare during transport and slaughter in Bulgaria – Impact of welfare on slaughter carcass and meat quality: a review. Turk J Vet Anim Sci, 44, 174-181.
- İnal T (1995): Kesim Hayvanı ve Et Muayenesi. Saray Kitabevleri, İstanbul.
- Kadim IT, Mahgoub O, Al-Marzooqi W, et al (2009): Effects of transportation during the hot season and low voltage electrical stimulation on histochemical and meat quality characteristics of sheep longissimus muscle. Livest Sci, 126, 154-161.
- Kara NK, Koyuncu M (2011): Sığırlarda taşıma sırasında hayvan refahına etki eden faktörler. Kafkas Univ Vet Fak Derg, 17, 5.
- Kerth CR, Braden KW, Cox R, et al (2007): Carcass, sensory, fat color, and consumer acceptance characteristics of Angus-cross steers finished on ryegrass (Lolium multiflorum) forage or on a high-concentrate diet. Meat Sci, 75, 324-331.
- Koohmaraie M (1996): Biochemical factors regulating the toughening and tenderization processes of meat. Meat Sci, 43S1, 193-201.
- Li CB, Chen YJ, Xu XL, et al (2010): Effects of low-voltage electrical stimulation and rapid chilling on meat quality characteristics of Chinese Yellow crossbred bulls. Meat Sci, 72, 9-17.
- Maria GA (2008). Long Distance Transport and Welfare of Farm Animals. 77-112 In: Meat Quality. CABI, UK.
- Maria GA, Villarroel M, Sanudo C, et al (2003): Effect of transport time and ageing on aspects of beef quality. Meat Sci, 65, 1335-1340.
- Mounier L, Dubroeucq H, Andanson S, et al (2006): Variations in meat pH of beef bulls in relation to conditions of transfer to slaughter and previous history of the animals. J Anim Sci, 84, 1567-1576.
- Najafi MH, Mohammadi Y, Najafi A, et al (2020): Lairage time effect on carcass traits, meat quality parameters and sensory properties of Mehraban fat-tailed lambs subjected to short distance transportation. Small Rumin Res, 188, 106122.
- Pfaffl MW (2001): A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res, 29, e45.
- Pophiwa P, Webb EC, Frylinck L (2020): A review of factors affecting goat meat quality and mitigating strategies. Small Rumin Res, 183, 106035.
- Schaefer AL, Jones SDM, Tong AKW, et al (1988): The effects of fasting and transportation on beef cattle. I. Acid-base-electrolyte balance and infrared heat loss of beef cattle. Livest Prod Sci, 20, 9.
- Teke B, Akdag F, Ekiz B, et al (2014): Effects of different lairage times after long distance transportation on carcass and meat quality characteristics of Hungarian Simmental bulls. Meat Sci, 96, 224-229.
- Terlouw EM, Arnould C, Auperin B, et al (2008): Pre-slaughter conditions, animal stress and welfare: current status and possible future research. Anim, 2, 1501-1517.
- Tomovic VM, Petrovic LS, Dzinic NR (2008): Effects of rapid chilling of carcasses and time of deboning on weight loss and technological quality of pork semimembranosus muscle. Meat Sci, 80, 1188-1193.
- Van de Water G, Verjans F, Geers R (2003): The effect of short distance transport under commercial conditions on the physiology of slaughter calves; pH and colour profiles of veal. Livest Prod Sci, 82, 8.
- Vapnek J, Chapman MS (2010): Legislative and regulatory options for animal welfare, FAO Legis Study, 104.
- Vergara H, Gallego L (2000): Effect of electrical stunning on meat quality of lamb. Meat Sci, 56, 345-349.
- Vergara H, Linares MB, Berruga MI, et al (2005): Meat quality in suckling lambs: effect of preslaughter handling. Meat Sci, 69, 5.
- Vural H, Öztan A (1996): Et ve Ürünleri Kalite Kontrol Laboratuarı Uygulama Kılavuzu. H Ü Mühendislik Fakültesi Yayınları, Ankara.
- Yalcintan H, Akin PD, Ozturk N, et al (2018): Effect of lairage time after 2 h transport on stress parameters and meat quality characteristics in Kivircik ewe lambs. Small Rumin Res, 166, 41-46.
- Yalcintan H, Öztürk N, Akin PD, et al (2020): Slaughter and carcass characteristics of Kivircik lambs in different rearing seasons. Ankara Univ Vet Fak Derg, 68, 91-95.
Year 2023,
, 401 - 412, 29.09.2023
Abstract
Project Number
116O654 and 25293
References
- Altınçekiç ŞÖ, Koyuncu, M (2010): Nakil koşullarının hayvan refahı üzerine etkileri. Hay Üret, 51, 8.
- AMSA (1991): American Meat Association Guidelines for Meat Colour Evaluation. Nat. Live Stock and Meat Board Chicago, USA.
- Bagatoli A, Gasparino E, Soares MA, et al (2013): Expression of calpastatin and myostatin genes associated with lamb meat quality. Genet Mol Res, 12, 4, 6168-6175.
- Bendall JR (1973): The biochemistry of rigor mortis and cold contracture. In Proceedings of the 19th European Meeting of Meat Research Workers. Paris, France.
- Calvo JH, Iguacel LP, Kirinus JK, et al (2014): A new single nucleotide polymorphism in the calpastatin (CAST) gene associated with beef tenderness. Meat Sci, 96, 775-782.
- Catts VS, Catts SV, Fernandez HR, et al (2005): Microarray study of post-mortem mRNA degradation in mouse brain tissue. Brain Res Mol Brain Res, 138, 164-177.
- Cerdeño AI, Vieira C, Serrano E, et al (2006): Carcass and meat quality in Brown fattened young bulls: effect of rearing method and slaughter weight. J Anim Feed Sci, 15, 7.
- Chulayo AY, Bradley G, Muchenje V (2016): Effects of transport distance, lairage time and stunning efficiency on cortisol, glucose, HSPA1A and how they relate with meat quality in cattle. Meat Sci, 117, 89-96.
- Cross HR, West RL, Dutson TR (1981): Comparison of methods for measuring sarcomere length in beef semitendinosus muscle. Meat Sci, 5, 261-266.
- Dangi SS, Gupta M, Nagar V, et al (2014). Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats. Int J Biometeorol, 58, 2085-2093.
- Del Campo M, Brito G, Soares de Lima J, et al (2010): Temperament and lairage time effects on carcass and meat quality traits in steers. Meat Sci, 86, 908-914.
- Diaz MT, Vieira C, Perez C, et al (2014): Effect of lairage time ( 0h, 3 h, 6 h or 12 h) on glycogen content and meat quality parameters in suckling lambs. Meat Sci, 96, 653-660.
- Ekiz B, Ekiz EE, Kocak O, et al (2012): Effect of pre-slaughter management regarding transportation and time in lairage on certain stress parameters, carcass and meat quality characteristics in Kivircik lambs. Meat Sci, 90, 967-976.
- Ferguson DM, Warner RD (2008): Have we underestimated the impact of pre-slaughter stress on meat quality in ruminants? Meat Sci, 80, 12-19.
- Ferreira GB, Andrade CL, Costa F, et al (2006): Effects of transport time and rest period on the quality of electrically stimulated male cattle carcasses. Meat Sci, 74, 459-466.
- Ferreira PG, Munoz-Aguirre M, Reverter F, et al (2018): The effects of death and post-mortem cold ischemia on human tissue transcriptomes. Nat Commun, 9, 490.
- Fitzpatrick R, Casey OM, Morris D, et al (2002): Postmortem stability of RNA isolated from bovine reproductive tissues. Biochim Biophys Acta, 1574, 10-14.
- Gallo C, Lizondo G, Knowles TG (2003): Effects of journey and lairage time on steers transported to slaughter in Chile. Vet Rec, 152, 361-364.
- Gandolfi G, Pomponio L, Ertbjerg P, et al (2011): Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality. Meat Sci, 88, 694-700.
- Hannula T, Puolanne E (2004): The effect of cooling rate on beef tenderness: The significance of pH at 7 degrees C. Meat Sci, 67, 403-408.
- HNSRKY (2014): Hayvan Nakilleri Sırasında Refah ve Korunması Yönetmeliği. Available at https://www.resmigazete.gov.tr/eskiler/2014/02/20140205-3.htm Resmi Gazete. (Accessed August 17, 2022).
- Holman BWB, Collins D, Kilgannon AK, et al (2020): Using shear force, sarcomere length, particle size, collagen content, and protein solubility metrics to predict consumer acceptance of aged beef tenderness. J Texture Stud, 51, 559-566.
- ISO (1985): Sensory Analysis – Methodology – General Guidance. International Organization for Standardization, Paris.
- ISO (1988): Sensory Analysis – General Guidance for Design of Test Rooms. International Organization for Standardization, Paris.
- ISO (1993): Sensory Analysis – General Guidance for the Selection, Training and Monitoring of Assessors. Part 1: Selected Assessors. International Organization for Standardization, Genève.
- Ivanov NT (2020): Sheep welfare during transport and slaughter in Bulgaria – Impact of welfare on slaughter carcass and meat quality: a review. Turk J Vet Anim Sci, 44, 174-181.
- İnal T (1995): Kesim Hayvanı ve Et Muayenesi. Saray Kitabevleri, İstanbul.
- Kadim IT, Mahgoub O, Al-Marzooqi W, et al (2009): Effects of transportation during the hot season and low voltage electrical stimulation on histochemical and meat quality characteristics of sheep longissimus muscle. Livest Sci, 126, 154-161.
- Kara NK, Koyuncu M (2011): Sığırlarda taşıma sırasında hayvan refahına etki eden faktörler. Kafkas Univ Vet Fak Derg, 17, 5.
- Kerth CR, Braden KW, Cox R, et al (2007): Carcass, sensory, fat color, and consumer acceptance characteristics of Angus-cross steers finished on ryegrass (Lolium multiflorum) forage or on a high-concentrate diet. Meat Sci, 75, 324-331.
- Koohmaraie M (1996): Biochemical factors regulating the toughening and tenderization processes of meat. Meat Sci, 43S1, 193-201.
- Li CB, Chen YJ, Xu XL, et al (2010): Effects of low-voltage electrical stimulation and rapid chilling on meat quality characteristics of Chinese Yellow crossbred bulls. Meat Sci, 72, 9-17.
- Maria GA (2008). Long Distance Transport and Welfare of Farm Animals. 77-112 In: Meat Quality. CABI, UK.
- Maria GA, Villarroel M, Sanudo C, et al (2003): Effect of transport time and ageing on aspects of beef quality. Meat Sci, 65, 1335-1340.
- Mounier L, Dubroeucq H, Andanson S, et al (2006): Variations in meat pH of beef bulls in relation to conditions of transfer to slaughter and previous history of the animals. J Anim Sci, 84, 1567-1576.
- Najafi MH, Mohammadi Y, Najafi A, et al (2020): Lairage time effect on carcass traits, meat quality parameters and sensory properties of Mehraban fat-tailed lambs subjected to short distance transportation. Small Rumin Res, 188, 106122.
- Pfaffl MW (2001): A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res, 29, e45.
- Pophiwa P, Webb EC, Frylinck L (2020): A review of factors affecting goat meat quality and mitigating strategies. Small Rumin Res, 183, 106035.
- Schaefer AL, Jones SDM, Tong AKW, et al (1988): The effects of fasting and transportation on beef cattle. I. Acid-base-electrolyte balance and infrared heat loss of beef cattle. Livest Prod Sci, 20, 9.
- Teke B, Akdag F, Ekiz B, et al (2014): Effects of different lairage times after long distance transportation on carcass and meat quality characteristics of Hungarian Simmental bulls. Meat Sci, 96, 224-229.
- Terlouw EM, Arnould C, Auperin B, et al (2008): Pre-slaughter conditions, animal stress and welfare: current status and possible future research. Anim, 2, 1501-1517.
- Tomovic VM, Petrovic LS, Dzinic NR (2008): Effects of rapid chilling of carcasses and time of deboning on weight loss and technological quality of pork semimembranosus muscle. Meat Sci, 80, 1188-1193.
- Van de Water G, Verjans F, Geers R (2003): The effect of short distance transport under commercial conditions on the physiology of slaughter calves; pH and colour profiles of veal. Livest Prod Sci, 82, 8.
- Vapnek J, Chapman MS (2010): Legislative and regulatory options for animal welfare, FAO Legis Study, 104.
- Vergara H, Gallego L (2000): Effect of electrical stunning on meat quality of lamb. Meat Sci, 56, 345-349.
- Vergara H, Linares MB, Berruga MI, et al (2005): Meat quality in suckling lambs: effect of preslaughter handling. Meat Sci, 69, 5.
- Vural H, Öztan A (1996): Et ve Ürünleri Kalite Kontrol Laboratuarı Uygulama Kılavuzu. H Ü Mühendislik Fakültesi Yayınları, Ankara.
- Yalcintan H, Akin PD, Ozturk N, et al (2018): Effect of lairage time after 2 h transport on stress parameters and meat quality characteristics in Kivircik ewe lambs. Small Rumin Res, 166, 41-46.
- Yalcintan H, Öztürk N, Akin PD, et al (2020): Slaughter and carcass characteristics of Kivircik lambs in different rearing seasons. Ankara Univ Vet Fak Derg, 68, 91-95.
There are 49 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Biochemistry, Veterinary Food Hygiene and Technology, Veterinary Histology and Embryology, Animal Science, Genetics and Biostatistics, Veterinary Sciences (Other) |
| Journal Section | Research Article |
| Authors | |
| Project Number | 116O654 and 25293 |
| Publication Date | September 29, 2023 |
| Published in Issue | Year 2023 |